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The map label for this gene is rob [H]

Identifier: 157372255

GI number: 157372255

Start: 4463351

End: 4464217

Strand: Reverse

Name: rob [H]

Synonym: Spro_4021

Alternate gene names: 157372255

Gene position: 4464217-4463351 (Counterclockwise)

Preceding gene: 157372256

Following gene: 157372252

Centisome position: 81.93

GC content: 51.9

Gene sequence:

>867_bases
ATGGAAACGCAAGAAAATCATATCAGGGAACTACTGGTCTGGATTGAAGACAACCTGACCAACCCGCTATCATTGGATAT
CGTTTCTGCCAAATCAGGCTACTCGAAATGGTATCTGCAGCGAATGTTCAAGACGCAGACCGGTCTGTCGCTGGCCTCCT
ATATTCGCGCCCGCCGTTTGTATCTCGCCGCTTTCGCCTTGCGCTTTACGCAAAAAACCATTCTTGATATTTCCATTGAA
TATCAATTTGATAACCAGCAAACCTTTTCCCGCTGCTTCAAAAAGCACTTTGCCGAATCTCCCAGCGTCTATCGTCATGC
CCGCAAGCAGGACTTCAGTCATCTGGTTCGTTCGCTGTCAGCCAACCATCCTGGTGATATTCAGGTTGACCGCATCAAGG
TGGCCAAAGGGCAGTATGCGATCAAGGGGAAAAGTTACGCTTACCACCTCGATATTGCCGATTTGGATAAGTCGCATTTG
CACATCCGCAGCAAAATCAGAGAGCAGTTCTATCAATCGGTGGCCAGCCGGCCGGCATTGACCTATGCATTGACCCAGTT
TATTCCTGATGGAGAGCAGGTCAGAGTGGAATATGCGCTTGGCGTTGCCGAGGACTGCCCGACCAGTGATGTCGCCGGGC
TTGAGCCATGGCCGGAAATTCACGGCGATTATTGCCGCTTTTGTTACTCCGGCAAGCCGGTGGCCTTGCACGACCATGTG
GTGCAGATTTACACCCAGGCACTACCGGAATTGGGCCTGGCCCGCAGAGTAGGGCCAGACCTGACGATGTTCAACTATTC
GCTGAACGAGCGGGAGGAGTTGCAGCTTGAGCTCCAGCATCTGGTGCCAGTGACTCCAATTGGTTGA

Upstream 100 bases:

>100_bases
CTGTAGCCCGCTGGACGCCGCGTTTTAACCTGTATACCCTACGTGCGCAGCAGTAATACACGAATAAAGGCATATTTGGC
ACAGAAGGCTCGGCAACTAC

Downstream 100 bases:

>100_bases
CCGATCGGACCGCGGTAGTGCGGTTTTTCTGCTGCACGATATCCGCCATTACCGCCAGCGCGATTTCCGCCGGGGTTTTA
CTGCCGAGTGGCAACCCGAT

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
METQENHIRELLVWIEDNLTNPLSLDIVSAKSGYSKWYLQRMFKTQTGLSLASYIRARRLYLAAFALRFTQKTILDISIE
YQFDNQQTFSRCFKKHFAESPSVYRHARKQDFSHLVRSLSANHPGDIQVDRIKVAKGQYAIKGKSYAYHLDIADLDKSHL
HIRSKIREQFYQSVASRPALTYALTQFIPDGEQVRVEYALGVAEDCPTSDVAGLEPWPEIHGDYCRFCYSGKPVALHDHV
VQIYTQALPELGLARRVGPDLTMFNYSLNEREELQLELQHLVPVTPIG

Sequences:

>Translated_288_residues
METQENHIRELLVWIEDNLTNPLSLDIVSAKSGYSKWYLQRMFKTQTGLSLASYIRARRLYLAAFALRFTQKTILDISIE
YQFDNQQTFSRCFKKHFAESPSVYRHARKQDFSHLVRSLSANHPGDIQVDRIKVAKGQYAIKGKSYAYHLDIADLDKSHL
HIRSKIREQFYQSVASRPALTYALTQFIPDGEQVRVEYALGVAEDCPTSDVAGLEPWPEIHGDYCRFCYSGKPVALHDHV
VQIYTQALPELGLARRVGPDLTMFNYSLNEREELQLELQHLVPVTPIG
>Mature_288_residues
METQENHIRELLVWIEDNLTNPLSLDIVSAKSGYSKWYLQRMFKTQTGLSLASYIRARRLYLAAFALRFTQKTILDISIE
YQFDNQQTFSRCFKKHFAESPSVYRHARKQDFSHLVRSLSANHPGDIQVDRIKVAKGQYAIKGKSYAYHLDIADLDKSHL
HIRSKIREQFYQSVASRPALTYALTQFIPDGEQVRVEYALGVAEDCPTSDVAGLEPWPEIHGDYCRFCYSGKPVALHDHV
VQIYTQALPELGLARRVGPDLTMFNYSLNEREELQLELQHLVPVTPIG

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=289, Percent_Identity=33.9100346020761, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1790497, Length=99, Percent_Identity=52.5252525252525, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87081928, Length=113, Percent_Identity=46.9026548672566, Blast_Score=104, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 33137; Mature: 33137

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METQENHIRELLVWIEDNLTNPLSLDIVSAKSGYSKWYLQRMFKTQTGLSLASYIRARRL
CCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
YLAAFALRFTQKTILDISIEYQFDNQQTFSRCFKKHFAESPSVYRHARKQDFSHLVRSLS
HHHHHHHHHHHHEEEEEEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ANHPGDIQVDRIKVAKGQYAIKGKSYAYHLDIADLDKSHLHIRSKIREQFYQSVASRPAL
CCCCCCEEEEEEEEECCCEEEECCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCHH
TYALTQFIPDGEQVRVEYALGVAEDCPTSDVAGLEPWPEIHGDYCRFCYSGKPVALHDHV
HHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHCCHHHHHHCCCCCEEHHHHH
VQIYTQALPELGLARRVGPDLTMFNYSLNEREELQLELQHLVPVTPIG
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
METQENHIRELLVWIEDNLTNPLSLDIVSAKSGYSKWYLQRMFKTQTGLSLASYIRARRL
CCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
YLAAFALRFTQKTILDISIEYQFDNQQTFSRCFKKHFAESPSVYRHARKQDFSHLVRSLS
HHHHHHHHHHHHEEEEEEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ANHPGDIQVDRIKVAKGQYAIKGKSYAYHLDIADLDKSHLHIRSKIREQFYQSVASRPAL
CCCCCCEEEEEEEEECCCEEEECCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCHH
TYALTQFIPDGEQVRVEYALGVAEDCPTSDVAGLEPWPEIHGDYCRFCYSGKPVALHDHV
HHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHCCHHHHHHCCCCCEEHHHHH
VQIYTQALPELGLARRVGPDLTMFNYSLNEREELQLELQHLVPVTPIG
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]