Click here to switch to the map view.

The map label for this gene is fumA [H]

Identifier: 157372256

GI number: 157372256

Start: 4464387

End: 4466033

Strand: Reverse

Name: fumA [H]

Synonym: Spro_4022

Alternate gene names: 157372256

Gene position: 4466033-4464387 (Counterclockwise)

Preceding gene: 157372263

Following gene: 157372255

Centisome position: 81.96

GC content: 56.16

Gene sequence:

>1647_bases
ATGTCGAATAAACCGTTCCATTATCAAGATCCGTTCCCACTGAAGAAAGACGATACTGAATACTATCTGTTGAGCCGTGA
CCACGTTTCCGTGAGTGAGTTTGAAGGCCAGGAAGTGCTGAAAGTAGCCCCCGAAGCGTTAACCCTCCTTGCTCAACATG
CATTCCATGACGCCTCATTCATGCTGCGCCCGGCGCATCAGCAGCAGGTGGCCGATATCCTTAAGGATCCGGACGCCAGC
GAAAACGACAAGTACGTTGCGCTGCAATTTTTACGTAACTCAGAAATCGCCGCCAAGGGTATTTTACCGACCTGTCAGGA
TACCGGTACCGCCATCATCGTCGGCAAAAAAGGCCAGCGCGTCTGGACCGGCGGCGGTGACGAAGCGGCATTGTCGCGTG
GGGTGTACAACACCTTTATCGAAGACAACCTGCGCTACTCGCAAAACGCCGCGCTGGATATGTACAACGAGGTCAACACC
GGCAGCAACCTGCCGGCGCAGATCGACCTCTACAGCGTTGACGGTGAAGAATACAAGTTCCTGTGCATCGCCAAGGGCGG
TGGTTCAGCCAATAAAACCTATCTTTACCAGGAAACCAAGGCGCTGCTGTCCCCGGGCAAGCTGAAGGATTATCTGGTCG
AGAAAATGCGTACGTTGGGTACCGCGGCCTGCCCGCCTTACCACGTGGCATTCGTTATCGGTGGTACCTCGGCCGAAGCG
ACGTTGAAAACCGTCAAGCTGGCCTCGACCAAGTACTATGACGGCCTGCCGACCGAAGGCAATGAGCACGGTCAGGCATT
CCGCGACGTAGCGCTGGAAGAAGAGTTACTCAAAGAAGCGCAGAATTTGGGGTTGGGCGCGCAGTTTGGCGGCAAATACT
TTGCGCACGACATTCGCGTGGTGCGTCTGCCGCGCCATGGTGCTTCCTGTCCGGTAGGCATGGGCGTTTCCTGCTCCGCT
GACCGTAACATCAAGGGCAAGATTAACCGTGACGGCATCTGGCTGGAGAAGCTGGAGCACAATCCGGGCAAGTTTATTCC
GCAAGAACTGCGTCAGGCGGGGGAAGGCGAAGCCGTCAAGATTGACCTCAACCGCCCAATGGCCGATATCCTCAAGCAGC
TGTCGCAATTCCCGGTGTCTACCCGTTTGTCGCTGAGTGGCACCATCATCGTGGGGCGCGACATTGCCCACGCCAAGCTG
AAAGAGCGGCTGGACAACGGCGAAGGTCTGCCGCAGTACGTTAAGGATCATCCGATTTACTACGCCGGCCCGGCCAAAAC
GCCGGACGGTTACGCTTCAGGTTCTCTGGGGCCAACCACCGCCGGGCGTATGGACTCTTACGTTGACCTGCTGCAGTCCC
ACGGCGGCAGTATGATCATGCTGGCGAAAGGCAACCGCAGCCAGCAAGTGACCGACGCCTGCCACAAACACGGCGGTTTT
TACCTCGGCAGTATTGGTGGCCCGGCGGCGGTGCTGGCGCAACAAAGCATTAAAAGCCTGACCTGCGTCGAATACCCTGA
ACTGGGGATGGAAGCTATTTGGAAAATCGAAGTGGAAGATTTCCCGGCGTTCATTCTGGTGGATGATAAAGGCAATGACT
TCTTCCAGAAGATCCAGGCCGGTCAGTGTTCACGCTGTGTGAAATAA

Upstream 100 bases:

>100_bases
TAAAAATACGTAAGGTGTGGGGAGTTTTTTGACGCGGCCTGCCCCGAGCTGGCTGAGTAAGTGTGAATTTAACGCAGCTG
ATGTTGCAAGTGAGAGATTA

Downstream 100 bases:

>100_bases
CTTTCGTCAATACTTGCCGTCCGCTTTCGGGCGGCAAGTTTCAGGCTTGCTCTGGCCCTGTGTAAAGTCCTGTAGCCCGC
TGGACGCCGCGTTTTAACCT

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase subunit beta

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSNKPFHYQDPFPLKKDDTEYYLLSRDHVSVSEFEGQEVLKVAPEALTLLAQHAFHDASFMLRPAHQQQVADILKDPDAS
ENDKYVALQFLRNSEIAAKGILPTCQDTGTAIIVGKKGQRVWTGGGDEAALSRGVYNTFIEDNLRYSQNAALDMYNEVNT
GSNLPAQIDLYSVDGEEYKFLCIAKGGGSANKTYLYQETKALLSPGKLKDYLVEKMRTLGTAACPPYHVAFVIGGTSAEA
TLKTVKLASTKYYDGLPTEGNEHGQAFRDVALEEELLKEAQNLGLGAQFGGKYFAHDIRVVRLPRHGASCPVGMGVSCSA
DRNIKGKINRDGIWLEKLEHNPGKFIPQELRQAGEGEAVKIDLNRPMADILKQLSQFPVSTRLSLSGTIIVGRDIAHAKL
KERLDNGEGLPQYVKDHPIYYAGPAKTPDGYASGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
YLGSIGGPAAVLAQQSIKSLTCVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQKIQAGQCSRCVK

Sequences:

>Translated_548_residues
MSNKPFHYQDPFPLKKDDTEYYLLSRDHVSVSEFEGQEVLKVAPEALTLLAQHAFHDASFMLRPAHQQQVADILKDPDAS
ENDKYVALQFLRNSEIAAKGILPTCQDTGTAIIVGKKGQRVWTGGGDEAALSRGVYNTFIEDNLRYSQNAALDMYNEVNT
GSNLPAQIDLYSVDGEEYKFLCIAKGGGSANKTYLYQETKALLSPGKLKDYLVEKMRTLGTAACPPYHVAFVIGGTSAEA
TLKTVKLASTKYYDGLPTEGNEHGQAFRDVALEEELLKEAQNLGLGAQFGGKYFAHDIRVVRLPRHGASCPVGMGVSCSA
DRNIKGKINRDGIWLEKLEHNPGKFIPQELRQAGEGEAVKIDLNRPMADILKQLSQFPVSTRLSLSGTIIVGRDIAHAKL
KERLDNGEGLPQYVKDHPIYYAGPAKTPDGYASGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
YLGSIGGPAAVLAQQSIKSLTCVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQKIQAGQCSRCVK
>Mature_547_residues
SNKPFHYQDPFPLKKDDTEYYLLSRDHVSVSEFEGQEVLKVAPEALTLLAQHAFHDASFMLRPAHQQQVADILKDPDASE
NDKYVALQFLRNSEIAAKGILPTCQDTGTAIIVGKKGQRVWTGGGDEAALSRGVYNTFIEDNLRYSQNAALDMYNEVNTG
SNLPAQIDLYSVDGEEYKFLCIAKGGGSANKTYLYQETKALLSPGKLKDYLVEKMRTLGTAACPPYHVAFVIGGTSAEAT
LKTVKLASTKYYDGLPTEGNEHGQAFRDVALEEELLKEAQNLGLGAQFGGKYFAHDIRVVRLPRHGASCPVGMGVSCSAD
RNIKGKINRDGIWLEKLEHNPGKFIPQELRQAGEGEAVKIDLNRPMADILKQLSQFPVSTRLSLSGTIIVGRDIAHAKLK
ERLDNGEGLPQYVKDHPIYYAGPAKTPDGYASGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGFY
LGSIGGPAAVLAQQSIKSLTCVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQKIQAGQCSRCVK

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=548, Percent_Identity=89.963503649635, Blast_Score=1047, Evalue=0.0,
Organism=Escherichia coli, GI1790564, Length=548, Percent_Identity=86.6788321167883, Blast_Score=1009, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=166, Percent_Identity=28.3132530120482, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 59946; Mature: 59814

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKPFHYQDPFPLKKDDTEYYLLSRDHVSVSEFEGQEVLKVAPEALTLLAQHAFHDASF
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCHH
MLRPAHQQQVADILKDPDASENDKYVALQFLRNSEIAAKGILPTCQDTGTAIIVGKKGQR
EECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHCCCCCCCCCCCCEEEEECCCCE
VWTGGGDEAALSRGVYNTFIEDNLRYSQNAALDMYNEVNTGSNLPAQIDLYSVDGEEYKF
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEEECCCCEEE
LCIAKGGGSANKTYLYQETKALLSPGKLKDYLVEKMRTLGTAACPPYHVAFVIGGTSAEA
EEEECCCCCCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCH
TLKTVKLASTKYYDGLPTEGNEHGQAFRDVALEEELLKEAQNLGLGAQFGGKYFAHDIRV
HEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEE
VRLPRHGASCPVGMGVSCSADRNIKGKINRDGIWLEKLEHNPGKFIPQELRQAGEGEAVK
EEECCCCCCCCCCCCEECCCCCCCCCEECCCCEEEEEECCCCCCCCHHHHHHCCCCCEEE
IDLNRPMADILKQLSQFPVSTRLSLSGTIIVGRDIAHAKLKERLDNGEGLPQYVKDHPIY
EECCCCHHHHHHHHHHCCCCEEEEECEEEEEECCHHHHHHHHHHCCCCCCHHHHHCCCEE
YAGPAKTPDGYASGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQQSIKSLTCVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQKIQA
EEECCCCHHHHHHHHHHHHCEECCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHC
GQCSRCVK
CCHHHCCC
>Mature Secondary Structure 
SNKPFHYQDPFPLKKDDTEYYLLSRDHVSVSEFEGQEVLKVAPEALTLLAQHAFHDASF
CCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCHH
MLRPAHQQQVADILKDPDASENDKYVALQFLRNSEIAAKGILPTCQDTGTAIIVGKKGQR
EECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHCCCCCCCCCCCCEEEEECCCCE
VWTGGGDEAALSRGVYNTFIEDNLRYSQNAALDMYNEVNTGSNLPAQIDLYSVDGEEYKF
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEEECCCCEEE
LCIAKGGGSANKTYLYQETKALLSPGKLKDYLVEKMRTLGTAACPPYHVAFVIGGTSAEA
EEEECCCCCCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCH
TLKTVKLASTKYYDGLPTEGNEHGQAFRDVALEEELLKEAQNLGLGAQFGGKYFAHDIRV
HEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEE
VRLPRHGASCPVGMGVSCSADRNIKGKINRDGIWLEKLEHNPGKFIPQELRQAGEGEAVK
EEECCCCCCCCCCCCEECCCCCCCCCEECCCCEEEEEECCCCCCCCHHHHHHCCCCCEEE
IDLNRPMADILKQLSQFPVSTRLSLSGTIIVGRDIAHAKLKERLDNGEGLPQYVKDHPIY
EECCCCHHHHHHHHHHCCCCEEEEECEEEEEECCHHHHHHHHHHCCCCCCHHHHHCCCEE
YAGPAKTPDGYASGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQQSIKSLTCVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQKIQA
EEECCCCHHHHHHHHHHHHCEECCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHC
GQCSRCVK
CCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695 [H]