Click here to switch to the map view.

The map label for this gene is folK [H]

Identifier: 157372222

GI number: 157372222

Start: 4423804

End: 4424304

Strand: Direct

Name: folK [H]

Synonym: Spro_3988

Alternate gene names: 157372222

Gene position: 4423804-4424304 (Clockwise)

Preceding gene: 157372221

Following gene: 157372225

Centisome position: 81.19

GC content: 59.08

Gene sequence:

>501_bases
ATGATCCGCGTTTATATCGCGCTGGGCAGCAACCTGGCGCACCCGCTACAGCAGGTGAAAGCCGCACTGGAAGCGCTGGA
GCATCTCCCGCGCACCCGGTTGATCGTCTGTTCTTCGTTTTACCGCACCAAGCCGCTGGGGCCGCAGAACCAGCCGGACT
TTCTCAATGCGGTGGTGGCACTGGATACCTTGCTGCCCGCCGAACAGTTACTCGATCACACCCAGGCGATTGAACGCAAT
CAGGGCCGCGTGCGCAAAGATGAGCGCTGGGGCCCACGCACCCTCGATCTGGATATCATGCTGTATGGCGAACAAGTGAT
CCATACCGATCGCCTGACGGTGCCGCACTACGGTCTGAAAGAGCGTGAGTTTATGCTCTATCCGTTGGCAGAAATCGCCC
CCGAGTTAGTCTTCCCGGACGGCGAATCGCTGGTCAGCTGCCTGAAGCGCGTACCGCAAAACGGCATGGAGCTTTGGCAC
CAACCCAGGCAAAAAGTCTGA

Upstream 100 bases:

>100_bases
GACAGAAAACCATGTTAAGTACTTTGGGTGACGATCCGGCACCGCGCCGTGCGCGTCAGCGCCGCCCTCGCCGTCGGACG
CCGCGTAAAGAAGGAGAATA

Downstream 100 bases:

>100_bases
GTCGGCGCAGGATATACAGAACGCCCCTACAGCGCTACCTGAGCAACAAAGCCCCCTTCAGGCCGATTGGCGAAGCTAAC
CCGCAGCCCGTGTAAACCGG

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MIRVYIALGSNLAHPLQQVKAALEALEHLPRTRLIVCSSFYRTKPLGPQNQPDFLNAVVALDTLLPAEQLLDHTQAIERN
QGRVRKDERWGPRTLDLDIMLYGEQVIHTDRLTVPHYGLKEREFMLYPLAEIAPELVFPDGESLVSCLKRVPQNGMELWH
QPRQKV

Sequences:

>Translated_166_residues
MIRVYIALGSNLAHPLQQVKAALEALEHLPRTRLIVCSSFYRTKPLGPQNQPDFLNAVVALDTLLPAEQLLDHTQAIERN
QGRVRKDERWGPRTLDLDIMLYGEQVIHTDRLTVPHYGLKEREFMLYPLAEIAPELVFPDGESLVSCLKRVPQNGMELWH
QPRQKV
>Mature_166_residues
MIRVYIALGSNLAHPLQQVKAALEALEHLPRTRLIVCSSFYRTKPLGPQNQPDFLNAVVALDTLLPAEQLLDHTQAIERN
QGRVRKDERWGPRTLDLDIMLYGEQVIHTDRLTVPHYGLKEREFMLYPLAEIAPELVFPDGESLVSCLKRVPQNGMELWH
QPRQKV

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=159, Percent_Identity=64.1509433962264, Blast_Score=209, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6324073, Length=156, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 19053; Mature: 19053

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVYIALGSNLAHPLQQVKAALEALEHLPRTRLIVCSSFYRTKPLGPQNQPDFLNAVVA
CEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LDTLLPAEQLLDHTQAIERNQGRVRKDERWGPRTLDLDIMLYGEQVIHTDRLTVPHYGLK
HHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCHHHCCCCCCCCCCCCC
EREFMLYPLAEIAPELVFPDGESLVSCLKRVPQNGMELWHQPRQKV
CCCEEEEEHHHHCHHHCCCCCHHHHHHHHHCCCCHHHHHHCHHCCC
>Mature Secondary Structure
MIRVYIALGSNLAHPLQQVKAALEALEHLPRTRLIVCSSFYRTKPLGPQNQPDFLNAVVA
CEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LDTLLPAEQLLDHTQAIERNQGRVRKDERWGPRTLDLDIMLYGEQVIHTDRLTVPHYGLK
HHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCHHHCCCCCCCCCCCCC
EREFMLYPLAEIAPELVFPDGESLVSCLKRVPQNGMELWHQPRQKV
CCCEEEEEHHHHCHHHCCCCCHHHHHHHHHCCCCHHHHHHCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1325970; 8202364; 9278503; 1657875; 2537812; 10378268; 10452528 [H]