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The map label for this gene is panB [H]

Identifier: 157372225

GI number: 157372225

Start: 4426452

End: 4427312

Strand: Direct

Name: panB [H]

Synonym: Spro_3991

Alternate gene names: 157372225

Gene position: 4426452-4427312 (Clockwise)

Preceding gene: 157372222

Following gene: 157372226

Centisome position: 81.24

GC content: 57.96

Gene sequence:

>861_bases
ATGACAGCACTTTTTTGCCGCCAGGGCCCTGCGTACACCGCGCGGCTCTATTCAGGAGACGACGTGATGAAACCCACCAC
CGTGACCCATTTGCACCAGTGGAAGCAGGAACAGCGCAAGTTTGCTTCCCTCACCGCCTACGATGCCAGCTTTGCCAAAC
TGTTTGAAGAGCAAGGCGTTAAAGTGCTGCTGGTGGGCGATTCACTGGGCATGACGCTGCAGGGCCACGAATCTACCCTA
CCGGTCACCGTCGCCGATATCGCCTATCATACCCGTGCCGTGCGCCGGGGTGCACCGGCCTGTCTGCTGTTGGCCGACCT
GCCCTTTATGAGCTATGCCACTCCACAGTTGGCCTGCGCCAATGCCGCCGAGCTGATGCAGGCCGGTGCCAATATGGTGA
AACTGGAAGGCGGCAGTTGGCTGTGTGAAACGGTGAAAACACTCACCGAACGCGCGGTGCCGGTCTGCGGCCATCTCGGC
CTGACACCGCAGTCAGTCAACGTATTCGGCGGCTACAAGGTGCAAGGCCGCGATGAACTTGCCGCCAAGCAACTGCTGGA
AGATGCAAAAAATCTGGAGCTGGCCGGTATCCAACTACTGGTGCTGGAGTGCGTACCAACCGAACTGGCACGCAAGATCA
CCGAAGCCCTGACCATTCCGGTGATCGGCATTGGTGCCGGTAACGCCACCGATGGACAGATCCTGGTGATGCACGACGCC
TTCGGCATTACCGGTGGCCACACGCCAAAATTCGCTAAAAACTTCCTGGCACAGAGCGGCGATATCCGCTCGGCGGTGCA
ACAATATATTCAAGACGTAGAGCAAGGCATTTACCCCGCTGAAGAGCACTCTTTCAACTAA

Upstream 100 bases:

>100_bases
CGTCCTTTTTTAGCCATTGCCGCCCATTACACCACAAGGTTCTTAAGAACCGCTTAAGCATTATGATCTGCGCTACCCAC
CCCGTTAGGGTTACAGTAGA

Downstream 100 bases:

>100_bases
AGTGTCCGCGTTAGGAGTTATGGAATGGTAATTATCGAAACCCTGCCGCTGTTGCGTCAGCAGATCCGCCGCTGGCGTCA
GGATGGGAAACGCATCGCGC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MTALFCRQGPAYTARLYSGDDVMKPTTVTHLHQWKQEQRKFASLTAYDASFAKLFEEQGVKVLLVGDSLGMTLQGHESTL
PVTVADIAYHTRAVRRGAPACLLLADLPFMSYATPQLACANAAELMQAGANMVKLEGGSWLCETVKTLTERAVPVCGHLG
LTPQSVNVFGGYKVQGRDELAAKQLLEDAKNLELAGIQLLVLECVPTELARKITEALTIPVIGIGAGNATDGQILVMHDA
FGITGGHTPKFAKNFLAQSGDIRSAVQQYIQDVEQGIYPAEEHSFN

Sequences:

>Translated_286_residues
MTALFCRQGPAYTARLYSGDDVMKPTTVTHLHQWKQEQRKFASLTAYDASFAKLFEEQGVKVLLVGDSLGMTLQGHESTL
PVTVADIAYHTRAVRRGAPACLLLADLPFMSYATPQLACANAAELMQAGANMVKLEGGSWLCETVKTLTERAVPVCGHLG
LTPQSVNVFGGYKVQGRDELAAKQLLEDAKNLELAGIQLLVLECVPTELARKITEALTIPVIGIGAGNATDGQILVMHDA
FGITGGHTPKFAKNFLAQSGDIRSAVQQYIQDVEQGIYPAEEHSFN
>Mature_285_residues
TALFCRQGPAYTARLYSGDDVMKPTTVTHLHQWKQEQRKFASLTAYDASFAKLFEEQGVKVLLVGDSLGMTLQGHESTLP
VTVADIAYHTRAVRRGAPACLLLADLPFMSYATPQLACANAAELMQAGANMVKLEGGSWLCETVKTLTERAVPVCGHLGL
TPQSVNVFGGYKVQGRDELAAKQLLEDAKNLELAGIQLLVLECVPTELARKITEALTIPVIGIGAGNATDGQILVMHDAF
GITGGHTPKFAKNFLAQSGDIRSAVQQYIQDVEQGIYPAEEHSFN

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate [H]

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family [H]

Homologues:

Organism=Escherichia coli, GI1786326, Length=264, Percent_Identity=76.1363636363636, Blast_Score=430, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6319653, Length=290, Percent_Identity=33.7931034482759, Blast_Score=166, Evalue=4e-42,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003700
- InterPro:   IPR015813 [H]

Pfam domain/function: PF02548 Pantoate_transf [H]

EC number: =2.1.2.11 [H]

Molecular weight: Translated: 30862; Mature: 30731

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALFCRQGPAYTARLYSGDDVMKPTTVTHLHQWKQEQRKFASLTAYDASFAKLFEEQGV
CCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KVLLVGDSLGMTLQGHESTLPVTVADIAYHTRAVRRGAPACLLLADLPFMSYATPQLACA
EEEEEECCCCCEEECCCCCCCEEHHHHHHHHHHHHCCCCEEEEEECCCHHHCCCCHHHHC
NAAELMQAGANMVKLEGGSWLCETVKTLTERAVPVCGHLGLTPQSVNVFGGYKVQGRDEL
CHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCHHCCCCCCCCCEEEECCEEECCHHHH
AAKQLLEDAKNLELAGIQLLVLECVPTELARKITEALTIPVIGIGAGNATDGQILVMHDA
HHHHHHHHHHCCCHHHHEEEEEHHCCHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEEEC
FGITGGHTPKFAKNFLAQSGDIRSAVQQYIQDVEQGIYPAEEHSFN
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TALFCRQGPAYTARLYSGDDVMKPTTVTHLHQWKQEQRKFASLTAYDASFAKLFEEQGV
CEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KVLLVGDSLGMTLQGHESTLPVTVADIAYHTRAVRRGAPACLLLADLPFMSYATPQLACA
EEEEEECCCCCEEECCCCCCCEEHHHHHHHHHHHHCCCCEEEEEECCCHHHCCCCHHHHC
NAAELMQAGANMVKLEGGSWLCETVKTLTERAVPVCGHLGLTPQSVNVFGGYKVQGRDEL
CHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCHHCCCCCCCCCEEEECCEEECCHHHH
AAKQLLEDAKNLELAGIQLLVLECVPTELARKITEALTIPVIGIGAGNATDGQILVMHDA
HHHHHHHHHHCCCHHHHEEEEEHHCCHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEEEC
FGITGGHTPKFAKNFLAQSGDIRSAVQQYIQDVEQGIYPAEEHSFN
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA