Click here to switch to the map view.

The map label for this gene is pcnB [H]

Identifier: 157372221

GI number: 157372221

Start: 4422287

End: 4423804

Strand: Direct

Name: pcnB [H]

Synonym: Spro_3987

Alternate gene names: 157372221

Gene position: 4422287-4423804 (Clockwise)

Preceding gene: 157372220

Following gene: 157372222

Centisome position: 81.16

GC content: 59.55

Gene sequence:

>1518_bases
GTGCTAATCCGCGATGACAAGCAACCGCGCGACGATGCGCCGGTCAGCGATAAAAACGTAGTCCGCGAAGAAAGCGTGGT
GGCGGAACAGCCGGCCGCGCCGCAGCGACGTTCCGTCGAACGCGGCAGAGAAAGCCCTGCCCGCCGGCCGGCACCGCGTA
AACGCCCCCCTTTTCCTGCCGCAGAGGGAACCGGCTCAATGACCGTGATCCCACGCGACCAGCATTCCATCTCACGTAAA
GACATCAGTGAGAATGCGCTGAAGGTGCTGTATCGCCTGAACAAATCCGGCTTCGAAGCCTATCTGGTCGGTGGCGGCGT
ACGCGACCTGCTGCTGGGCAAAAAACCGAAAGATTTCGACATCACCACCAACGCGACGCCGGAGCAAGTGCGCAAGCTGT
TCCGCAACTGCCGTCTGGTTGGCCGCCGCTTCCGCCTGGCCCACGTGATGTTCGGGCCGGAAATCATTGAGGTTGCCACC
TTCCGTGGTCACCACGAGCAGCAAACGCCGGAGTCTGACAAAAATTCTTCCCAGCAGGCCCAAAACGGCATGCTGCTGCG
CGACAACATCTTTGGTTCGATCGAAGAAGATGCCCAGCGCCGCGACTTCACCATCAACAGCCTGTATTACGGCGTAGCGG
ACTTCACCCTGCGTGATTACGTCGATGGCCTGCGCGATCTTAAACAGGGCGTGATCCGCCTGATTGGCGATCCGGAAACC
CGTTATCGTGAAGACCCGGTGCGTATGCTGCGCGCGGTGCGCTTTGCCGCCAAGCTGGACATGACCATCAGCGATGAAAC
CGCCGAGCCAATCCCGCGTCTGGCGTCCTTGCTGCATGAGATCCCGCCGGCGCGTCTGTTTGAGGAATCACTCAAGCTGC
TGCAGGCAGGCTACGGTTACCCGACCTATCTGAAACTCTGTGAATACCAGCTGTTCCAGCCGCTGTTCCCGCAGATTGCA
CGTCACTTCACGCCGGGTCAGGACACGCCAATGGAGCGTATTCTGGCGCAGGTGCTGAAGAACACCGACTACCGTCTGCA
AAACAACATGCGCGTCAATCCGGCGTTCCTGTTTGCTGCTATGCTTTGGTATCCGCTGCTGGAACATGCGCAGAAACTGG
CGCAGGAAAGTGGGCTGGCTTACTACGACGCCTTCGCGCTGGCGATGAATGATGTGTTGGACGAGCAGTGTCGTTCGCTG
GCCATTCCGAAACGCATCACCACGCTGGTTCGCGATATCTGGCAGTTGCAGCTGCGTCTGTCCCGCCGTCAGGGCAAGCG
TGCGCACAAGCTGATGGAACATCCGAAATTCCGCGCCGCTTACGATTTGCTGGCGCTGCGTGCCGAAGTAGAAGAAAACC
AGGAGATGCTGCGTCTGACCGAATGGTGGGGCGAGTTCCAGGAAGCCACACCGGCACGACAGAAAACCATGTTAAGTACT
TTGGGTGACGATCCGGCACCGCGCCGTGCGCGTCAGCGCCGCCCTCGCCGTCGGACGCCGCGTAAAGAAGGAGAATAA

Upstream 100 bases:

>100_bases
TGGTGAGCTATGATTAGCCGCTATTTTTTGTTAGCGCCATTTTTATCAGACACTATCGAGGTGTACCATTTTTACCCGAG
TAGCCAATTTCTGCCGTAAG

Downstream 100 bases:

>100_bases
TGATCCGCGTTTATATCGCGCTGGGCAGCAACCTGGCGCACCCGCTACAGCAGGTGAAAGCCGCACTGGAAGCGCTGGAG
CATCTCCCGCGCACCCGGTT

Product: poly(A) polymerase I

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MLIRDDKQPRDDAPVSDKNVVREESVVAEQPAAPQRRSVERGRESPARRPAPRKRPPFPAAEGTGSMTVIPRDQHSISRK
DISENALKVLYRLNKSGFEAYLVGGGVRDLLLGKKPKDFDITTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVAT
FRGHHEQQTPESDKNSSQQAQNGMLLRDNIFGSIEEDAQRRDFTINSLYYGVADFTLRDYVDGLRDLKQGVIRLIGDPET
RYREDPVRMLRAVRFAAKLDMTISDETAEPIPRLASLLHEIPPARLFEESLKLLQAGYGYPTYLKLCEYQLFQPLFPQIA
RHFTPGQDTPMERILAQVLKNTDYRLQNNMRVNPAFLFAAMLWYPLLEHAQKLAQESGLAYYDAFALAMNDVLDEQCRSL
AIPKRITTLVRDIWQLQLRLSRRQGKRAHKLMEHPKFRAAYDLLALRAEVEENQEMLRLTEWWGEFQEATPARQKTMLST
LGDDPAPRRARQRRPRRRTPRKEGE

Sequences:

>Translated_505_residues
MLIRDDKQPRDDAPVSDKNVVREESVVAEQPAAPQRRSVERGRESPARRPAPRKRPPFPAAEGTGSMTVIPRDQHSISRK
DISENALKVLYRLNKSGFEAYLVGGGVRDLLLGKKPKDFDITTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVAT
FRGHHEQQTPESDKNSSQQAQNGMLLRDNIFGSIEEDAQRRDFTINSLYYGVADFTLRDYVDGLRDLKQGVIRLIGDPET
RYREDPVRMLRAVRFAAKLDMTISDETAEPIPRLASLLHEIPPARLFEESLKLLQAGYGYPTYLKLCEYQLFQPLFPQIA
RHFTPGQDTPMERILAQVLKNTDYRLQNNMRVNPAFLFAAMLWYPLLEHAQKLAQESGLAYYDAFALAMNDVLDEQCRSL
AIPKRITTLVRDIWQLQLRLSRRQGKRAHKLMEHPKFRAAYDLLALRAEVEENQEMLRLTEWWGEFQEATPARQKTMLST
LGDDPAPRRARQRRPRRRTPRKEGE
>Mature_505_residues
MLIRDDKQPRDDAPVSDKNVVREESVVAEQPAAPQRRSVERGRESPARRPAPRKRPPFPAAEGTGSMTVIPRDQHSISRK
DISENALKVLYRLNKSGFEAYLVGGGVRDLLLGKKPKDFDITTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVAT
FRGHHEQQTPESDKNSSQQAQNGMLLRDNIFGSIEEDAQRRDFTINSLYYGVADFTLRDYVDGLRDLKQGVIRLIGDPET
RYREDPVRMLRAVRFAAKLDMTISDETAEPIPRLASLLHEIPPARLFEESLKLLQAGYGYPTYLKLCEYQLFQPLFPQIA
RHFTPGQDTPMERILAQVLKNTDYRLQNNMRVNPAFLFAAMLWYPLLEHAQKLAQESGLAYYDAFALAMNDVLDEQCRSL
AIPKRITTLVRDIWQLQLRLSRRQGKRAHKLMEHPKFRAAYDLLALRAEVEENQEMLRLTEWWGEFQEATPARQKTMLST
LGDDPAPRRARQRRPRRRTPRKEGE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=218, Percent_Identity=32.1100917431193, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI87081691, Length=418, Percent_Identity=81.8181818181818, Blast_Score=713, Evalue=0.0,
Organism=Escherichia coli, GI1789436, Length=229, Percent_Identity=30.1310043668122, Blast_Score=69, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321016, Length=190, Percent_Identity=28.4210526315789, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281360112, Length=237, Percent_Identity=29.957805907173, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21357337, Length=237, Percent_Identity=29.957805907173, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 58313; Mature: 58313

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIRDDKQPRDDAPVSDKNVVREESVVAEQPAAPQRRSVERGRESPARRPAPRKRPPFPA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
AEGTGSMTVIPRDQHSISRKDISENALKVLYRLNKSGFEAYLVGGGVRDLLLGKKPKDFD
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCC
ITTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEQQTPESDKNSSQQA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHH
QNGMLLRDNIFGSIEEDAQRRDFTINSLYYGVADFTLRDYVDGLRDLKQGVIRLIGDPET
HCCEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RYREDPVRMLRAVRFAAKLDMTISDETAEPIPRLASLLHEIPPARLFEESLKLLQAGYGY
HHHCCHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
PTYLKLCEYQLFQPLFPQIARHFTPGQDTPMERILAQVLKNTDYRLQNNMRVNPAFLFAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCHHHHHHH
MLWYPLLEHAQKLAQESGLAYYDAFALAMNDVLDEQCRSLAIPKRITTLVRDIWQLQLRL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
SRRQGKRAHKLMEHPKFRAAYDLLALRAEVEENQEMLRLTEWWGEFQEATPARQKTMLST
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LGDDPAPRRARQRRPRRRTPRKEGE
HCCCCCCHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MLIRDDKQPRDDAPVSDKNVVREESVVAEQPAAPQRRSVERGRESPARRPAPRKRPPFPA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
AEGTGSMTVIPRDQHSISRKDISENALKVLYRLNKSGFEAYLVGGGVRDLLLGKKPKDFD
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCC
ITTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEQQTPESDKNSSQQA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHH
QNGMLLRDNIFGSIEEDAQRRDFTINSLYYGVADFTLRDYVDGLRDLKQGVIRLIGDPET
HCCEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RYREDPVRMLRAVRFAAKLDMTISDETAEPIPRLASLLHEIPPARLFEESLKLLQAGYGY
HHHCCHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
PTYLKLCEYQLFQPLFPQIARHFTPGQDTPMERILAQVLKNTDYRLQNNMRVNPAFLFAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCHHHHHHH
MLWYPLLEHAQKLAQESGLAYYDAFALAMNDVLDEQCRSLAIPKRITTLVRDIWQLQLRL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
SRRQGKRAHKLMEHPKFRAAYDLLALRAEVEENQEMLRLTEWWGEFQEATPARQKTMLST
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LGDDPAPRRARQRRPRRRTPRKEGE
HCCCCCCHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]