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The map label for this gene is gluQ

Identifier: 157372220

GI number: 157372220

Start: 4421281

End: 4422192

Strand: Direct

Name: gluQ

Synonym: Spro_3986

Alternate gene names: 157372220

Gene position: 4421281-4422192 (Clockwise)

Preceding gene: 157372219

Following gene: 157372221

Centisome position: 81.14

GC content: 59.32

Gene sequence:

>912_bases
ATGCAAGAAAGACAATATGTGGGGCGCTTTGCCCCATCGCCTTCCGGAAGCCTGCATTTTGGTTCGCTGATTGCCGCTCT
GGGAAGCTACCTCCAGGCTCGTGCCCAACGCGGGCAGTGGCTGGTTCGCATTGAAGATATCGATCCCCCGCGCGAAGTCG
CCGGTGCGGCCGATCGTATCTTATCCGCTCTGGAACACTATGGTCTCCTCTGGGACGGCCAGGTCATCTATCAATCCCAA
CGTCATGAAGCCTACCGCGCTACTTTGGATCTACTGCATCAGCAGGGGCTCAGCTATTGCTGCAACTGCACCCGCAGCCG
TATCCAGCAGCTAGGCGGTCTGTATGACGGCCACTGTCGTCATTTGAATCTTGGCCCGCAGGGTGCCGCCATTCGCTTAC
GCCAGTCCACCCCGGTTTACGCCTTTCATGACCGCCTGCAGGGCGAGTTGCAGGCCGACCCGGCGCTGGCACGGGAAGAC
TTTATCATCCGCCGACGCGATGGGCTGTTCGCCTATAATCTGGCGGTGGTGGTGGACGACCATTTTCAGGGCGTTACCGA
AATTGTGCGTGGTGCCGACCTGATTGAACCGACGGTGCGCCAGATAGCGCTGTACCACCAGTTGCAGGCGCCGGTACCCA
CCTATGTACATCTGCCGCTGGCGCTGGGCGCTAACGGCAGCAAACTGTCGAAGCAAAACCATGCGCCCGCTCTCCCCGAC
GGCGATCCACGGCCGACCCTGGTGGCCGCGCTGAAATTTCTGCGTCAACCGCTGCCGGAAAGCTGGCAAGATCTTGACCT
GTCGTTATTATTGAGCTGGGCAGTCGCTCACTGGAAGCTGGAGGATGTGCCACGCCGGGAGGCTATTTCCCTGGACGAAA
ACACATCGGCATTCTCAAAAGAGCCATGGTGA

Upstream 100 bases:

>100_bases
AAATCCGCGAAAAGCAGATGGCAGGCTAATACAGTTGTTACCGCTGCGGCGTGCGTTCTGCGTGCGCCGCACAGAGAAAA
GGGAAAGTTTCCCCTCTGAT

Downstream 100 bases:

>100_bases
GCTATGATTAGCCGCTATTTTTTGTTAGCGCCATTTTTATCAGACACTATCGAGGTGTACCATTTTTACCCGAGTAGCCA
ATTTCTGCCGTAAGGTGCTA

Product: glutamate--tRNA ligase

Products: NA

Alternate protein names: Glu-Q-RSs

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MQERQYVGRFAPSPSGSLHFGSLIAALGSYLQARAQRGQWLVRIEDIDPPREVAGAADRILSALEHYGLLWDGQVIYQSQ
RHEAYRATLDLLHQQGLSYCCNCTRSRIQQLGGLYDGHCRHLNLGPQGAAIRLRQSTPVYAFHDRLQGELQADPALARED
FIIRRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIALYHQLQAPVPTYVHLPLALGANGSKLSKQNHAPALPD
GDPRPTLVAALKFLRQPLPESWQDLDLSLLLSWAVAHWKLEDVPRREAISLDENTSAFSKEPW

Sequences:

>Translated_303_residues
MQERQYVGRFAPSPSGSLHFGSLIAALGSYLQARAQRGQWLVRIEDIDPPREVAGAADRILSALEHYGLLWDGQVIYQSQ
RHEAYRATLDLLHQQGLSYCCNCTRSRIQQLGGLYDGHCRHLNLGPQGAAIRLRQSTPVYAFHDRLQGELQADPALARED
FIIRRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIALYHQLQAPVPTYVHLPLALGANGSKLSKQNHAPALPD
GDPRPTLVAALKFLRQPLPESWQDLDLSLLLSWAVAHWKLEDVPRREAISLDENTSAFSKEPW
>Mature_303_residues
MQERQYVGRFAPSPSGSLHFGSLIAALGSYLQARAQRGQWLVRIEDIDPPREVAGAADRILSALEHYGLLWDGQVIYQSQ
RHEAYRATLDLLHQQGLSYCCNCTRSRIQQLGGLYDGHCRHLNLGPQGAAIRLRQSTPVYAFHDRLQGELQADPALARED
FIIRRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIALYHQLQAPVPTYVHLPLALGANGSKLSKQNHAPALPD
GDPRPTLVAALKFLRQPLPESWQDLDLSLLLSWAVAHWKLEDVPRREAISLDENTSAFSKEPW

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily

Homologues:

Organism=Homo sapiens, GI134288884, Length=249, Percent_Identity=35.3413654618474, Blast_Score=132, Evalue=6e-31,
Organism=Escherichia coli, GI226510925, Length=290, Percent_Identity=68.6206896551724, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI1788743, Length=244, Percent_Identity=36.0655737704918, Blast_Score=149, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71993394, Length=250, Percent_Identity=31.6, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6324540, Length=269, Percent_Identity=33.457249070632, Blast_Score=141, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24665321, Length=249, Percent_Identity=34.5381526104418, Blast_Score=128, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLUQ_SERP5 (A8GIZ1)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480209.1
- ProteinModelPortal:   A8GIZ1
- SMR:   A8GIZ1
- STRING:   A8GIZ1
- GeneID:   5602803
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3986
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   IIKRSDG
- BioCyc:   SPRO399741:SPRO_3986-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01428
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR03838

Pfam domain/function: PF00749 tRNA-synt_1c

EC number: NA

Molecular weight: Translated: 33973; Mature: 33973

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 45-45 BINDING 172-172 BINDING 190-190 BINDING 231-231

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQERQYVGRFAPSPSGSLHFGSLIAALGSYLQARAQRGQWLVRIEDIDPPREVAGAADRI
CCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHH
LSALEHYGLLWDGQVIYQSQRHEAYRATLDLLHQQGLSYCCNCTRSRIQQLGGLYDGHCR
HHHHHHCCCEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
HLNLGPQGAAIRLRQSTPVYAFHDRLQGELQADPALAREDFIIRRRDGLFAYNLAVVVDD
EECCCCCCCEEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHEEEECCCEEEEEEEEEECC
HFQGVTEIVRGADLIEPTVRQIALYHQLQAPVPTYVHLPLALGANGSKLSKQNHAPALPD
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
GDPRPTLVAALKFLRQPLPESWQDLDLSLLLSWAVAHWKLEDVPRREAISLDENTSAFSK
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCC
EPW
CCC
>Mature Secondary Structure
MQERQYVGRFAPSPSGSLHFGSLIAALGSYLQARAQRGQWLVRIEDIDPPREVAGAADRI
CCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHH
LSALEHYGLLWDGQVIYQSQRHEAYRATLDLLHQQGLSYCCNCTRSRIQQLGGLYDGHCR
HHHHHHCCCEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
HLNLGPQGAAIRLRQSTPVYAFHDRLQGELQADPALAREDFIIRRRDGLFAYNLAVVVDD
EECCCCCCCEEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHEEEECCCEEEEEEEEEECC
HFQGVTEIVRGADLIEPTVRQIALYHQLQAPVPTYVHLPLALGANGSKLSKQNHAPALPD
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
GDPRPTLVAALKFLRQPLPESWQDLDLSLLLSWAVAHWKLEDVPRREAISLDENTSAFSK
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCC
EPW
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA