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The map label for this gene is ascG [H]

Identifier: 157372069

GI number: 157372069

Start: 4244638

End: 4245648

Strand: Direct

Name: ascG [H]

Synonym: Spro_3834

Alternate gene names: 157372069

Gene position: 4244638-4245648 (Clockwise)

Preceding gene: 157372067

Following gene: 157372070

Centisome position: 77.9

GC content: 59.35

Gene sequence:

>1011_bases
ATGATCACCATGTTGGATGTTTCCATTCGCGCGGGGGTATCTAAAGCCACGGTTTCACGCGTATTGAATGGCACCGGCCA
GGTGAAAAAGAGCACCCGCGACGCGGTATTCAAGGCCATGGACGACCTGGGCTACCGCCCCAATTTCCTGGCCCAATCGC
TGGCAAATAAAAGCTCCAACAGTATCGGGCTGGTGGTTTCCAACTTCGATGGTCCCTACTTCGGCCGCCTGCTGCGAAAA
GCCGCCAAGCTGATCGAGGCCAGCGGCAAGCATCTGATTGTCACCGATGGCCACGACACGCCGGAAGACGAGCTGCAGGC
GGTGCAACTGCTGGCCGATCGCCGCTGCGACGCCATCATCCTGTATACCCGTTTTATGTCCGAGCCCACGCTGATGAACC
TGTTGGACAGCCTGCCGGTGCCGATCATGGTGATCAACCGCGATCTGCCACAGGCCCGCGCACGCTGTGTGTCCTTCGAA
CAGCAGAAAGCCGCCTTCCATGCGGTGGATTATTTGATTCGGCAAGGACATCGCGAGATAGCCTGCATTACCGGGCCGAT
TGCCACGCCTACCGCCCTGGCTCGGTTAGCCGGTTATCGGCAGGCGCTTGAGCATCACACCATTGCCTTTGACGACGCCC
GCGTAGCCTATGGTGACAGCAGCGTACAGAGCGGCCATCAGGCTGTGCGTAGTTTACTGGCCGGGGGTAGCGGCTTTAGC
GCCCTGTTTGCCTGTAACGACGACATGGCGGTCGGCGCAATGAAAGCGTTGCACGATGCGGGTAAACGCCTGCCGCAGGA
TGTCTCACTGTTCGGCTTTGACGACGAACCCAGCGCCGCCTATTTACAACCGGCGCTGTCTACCGTTTATCTGCCGATCG
ACGTGATGATCGAAGCCGCCATCAGCCAGGCTTTCCGCCTGATTAAGGGGGAGGACATACAGCCACTTACGCCTTTCACC
GGTGAACTTCGGCTGCGGGAGTCCGTGGTGCCAGGGCCTTACGCCGACTGA

Upstream 100 bases:

>100_bases
AACGCAATCACTGCGATAAAAAGCCGCGCAGCCAAGGGCTAACCCTATGCTAGACTGAGCATTATTTGCCACAGCGGATG
TTGAAAGGGGTAAGCCATTG

Downstream 100 bases:

>100_bases
TTCCATCTGGCGCCGCCGCCGATTGCGCGCCGCGCCGCATTTCCCTTCCCTGCCACCAGCCCGCATATTGTCATGACAAT
AACCTTTACACTATATAGCA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Cryptic asc operon repressor [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MITMLDVSIRAGVSKATVSRVLNGTGQVKKSTRDAVFKAMDDLGYRPNFLAQSLANKSSNSIGLVVSNFDGPYFGRLLRK
AAKLIEASGKHLIVTDGHDTPEDELQAVQLLADRRCDAIILYTRFMSEPTLMNLLDSLPVPIMVINRDLPQARARCVSFE
QQKAAFHAVDYLIRQGHREIACITGPIATPTALARLAGYRQALEHHTIAFDDARVAYGDSSVQSGHQAVRSLLAGGSGFS
ALFACNDDMAVGAMKALHDAGKRLPQDVSLFGFDDEPSAAYLQPALSTVYLPIDVMIEAAISQAFRLIKGEDIQPLTPFT
GELRLRESVVPGPYAD

Sequences:

>Translated_336_residues
MITMLDVSIRAGVSKATVSRVLNGTGQVKKSTRDAVFKAMDDLGYRPNFLAQSLANKSSNSIGLVVSNFDGPYFGRLLRK
AAKLIEASGKHLIVTDGHDTPEDELQAVQLLADRRCDAIILYTRFMSEPTLMNLLDSLPVPIMVINRDLPQARARCVSFE
QQKAAFHAVDYLIRQGHREIACITGPIATPTALARLAGYRQALEHHTIAFDDARVAYGDSSVQSGHQAVRSLLAGGSGFS
ALFACNDDMAVGAMKALHDAGKRLPQDVSLFGFDDEPSAAYLQPALSTVYLPIDVMIEAAISQAFRLIKGEDIQPLTPFT
GELRLRESVVPGPYAD
>Mature_336_residues
MITMLDVSIRAGVSKATVSRVLNGTGQVKKSTRDAVFKAMDDLGYRPNFLAQSLANKSSNSIGLVVSNFDGPYFGRLLRK
AAKLIEASGKHLIVTDGHDTPEDELQAVQLLADRRCDAIILYTRFMSEPTLMNLLDSLPVPIMVINRDLPQARARCVSFE
QQKAAFHAVDYLIRQGHREIACITGPIATPTALARLAGYRQALEHHTIAFDDARVAYGDSSVQSGHQAVRSLLAGGSGFS
ALFACNDDMAVGAMKALHDAGKRLPQDVSLFGFDDEPSAAYLQPALSTVYLPIDVMIEAAISQAFRLIKGEDIQPLTPFT
GELRLRESVVPGPYAD

Specific function: Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=332, Percent_Identity=46.3855421686747, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1789202, Length=296, Percent_Identity=36.1486486486487, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1788474, Length=315, Percent_Identity=35.5555555555556, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1787948, Length=343, Percent_Identity=33.2361516034985, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1790369, Length=290, Percent_Identity=31.0344827586207, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1790194, Length=326, Percent_Identity=30.0613496932515, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787580, Length=316, Percent_Identity=27.5316455696203, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1786540, Length=321, Percent_Identity=29.9065420560748, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI48994940, Length=319, Percent_Identity=27.2727272727273, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787906, Length=283, Percent_Identity=28.6219081272085, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790715, Length=294, Percent_Identity=26.1904761904762, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789456, Length=336, Percent_Identity=25, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790192, Length=278, Percent_Identity=25.5395683453237, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786268, Length=295, Percent_Identity=25.4237288135593, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790689, Length=293, Percent_Identity=23.2081911262799, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36461; Mature: 36461

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITMLDVSIRAGVSKATVSRVLNGTGQVKKSTRDAVFKAMDDLGYRPNFLAQSLANKSSN
CEEEEEEHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
SIGLVVSNFDGPYFGRLLRKAAKLIEASGKHLIVTDGHDTPEDELQAVQLLADRRCDAII
CEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEE
LYTRFMSEPTLMNLLDSLPVPIMVINRDLPQARARCVSFEQQKAAFHAVDYLIRQGHREI
EEHHHHCCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ACITGPIATPTALARLAGYRQALEHHTIAFDDARVAYGDSSVQSGHQAVRSLLAGGSGFS
EEEECCCCCHHHHHHHHHHHHHHHHCCEEECCCEEEECCHHHHHHHHHHHHHHCCCCCCE
ALFACNDDMAVGAMKALHDAGKRLPQDVSLFGFDDEPSAAYLQPALSTVYLPIDVMIEAA
EEEEECCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHEEEHHHHHHHHH
ISQAFRLIKGEDIQPLTPFTGELRLRESVVPGPYAD
HHHHHHHHCCCCCCCCCCCCCCEEEHHCCCCCCCCC
>Mature Secondary Structure
MITMLDVSIRAGVSKATVSRVLNGTGQVKKSTRDAVFKAMDDLGYRPNFLAQSLANKSSN
CEEEEEEHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
SIGLVVSNFDGPYFGRLLRKAAKLIEASGKHLIVTDGHDTPEDELQAVQLLADRRCDAII
CEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEE
LYTRFMSEPTLMNLLDSLPVPIMVINRDLPQARARCVSFEQQKAAFHAVDYLIRQGHREI
EEHHHHCCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ACITGPIATPTALARLAGYRQALEHHTIAFDDARVAYGDSSVQSGHQAVRSLLAGGSGFS
EEEECCCCCHHHHHHHHHHHHHHHHCCEEECCCEEEECCHHHHHHHHHHHHHHCCCCCCE
ALFACNDDMAVGAMKALHDAGKRLPQDVSLFGFDDEPSAAYLQPALSTVYLPIDVMIEAA
EEEEECCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHEEEHHHHHHHHH
ISQAFRLIKGEDIQPLTPFTGELRLRESVVPGPYAD
HHHHHHHHCCCCCCCCCCCCCCEEEHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]