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The map label for this gene is mutH

Identifier: 157372060

GI number: 157372060

Start: 4233367

End: 4234053

Strand: Direct

Name: mutH

Synonym: Spro_3825

Alternate gene names: 157372060

Gene position: 4233367-4234053 (Clockwise)

Preceding gene: 157372047

Following gene: 157372061

Centisome position: 77.69

GC content: 58.95

Gene sequence:

>687_bases
ATGGCGTTTTTATCTCCTTTGCCCCCTCCTCCAGAAAACGAACAACAGCTGTTTGAAAGGGCGCAGCAGCTTGCCGGCTG
TTCCCTGGGCGAACTGGCCGAAGGTGCGCGGTTGGCTATCCCCAGGGATCTTAAGCGTGATAAAGGCTGGGTTGGCATGC
TGCTGGAGCAATATCTTGGCGCCATGGCAGGCAGCAAGCCGGAGCAGGATTTCCCCGAACTGGGCATCGAGCTGAAAACC
ATCCCGATAGACGCCGCCGGCAAACCGCTGGAAACCACCTTCGTCTGCGTAGCCCCCTTAACCGGCAACAGCGGCGTCAC
CTGGGCCAGCAGCCATGTACGTCATAAGCTGGCGCGGGTGCTGTGGATCCCGGTAGAAGGTGAGCGACAAATCCCGCTGG
CGCAGCGACGTATCGGTTCACCGCTGTTGTGGAGCCCAAACGCCGAAGAAGAAGAGATGCTGCGCCGCGACTGGGAGGAA
CTGATGGATTTGATCGTGCTCGGCCACGTAGAGCGGATCACCGCCCGCCACGGTGAGGTGCTGCAACTGCGTCCCAAGGC
GGCCAACAGTAAGGCCCTGACCGAAGCTATCGGCGAACAGGGGCAGCCGATCCTCACATTGCCACGCGGTTTTTACCTGA
AAAAAAGCTTCACCGGCGCATTATTGGCAAGACATTTCTTTGTCTGA

Upstream 100 bases:

>100_bases
AAATCATGCTACTATTATTCTTTTCCCCAGTCCTTTCCGGTCAAAGTTCTCTGTGGACAAATACAGTACTTTTTTCAACC
AAATCTAACAGGCTTGTATT

Downstream 100 bases:

>100_bases
TCCCTTTTGTTTAGGTCGCGTTTAGCTAACAGTGTCGTTGTATCAATAAACGCGTATAATTCAGCCTTTGTTTTTTATTA
AGGTGTGGCTCGACAATGTT

Product: DNA mismatch repair protein

Products: NA

Alternate protein names: Methyl-directed mismatch repair protein

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MAFLSPLPPPPENEQQLFERAQQLAGCSLGELAEGARLAIPRDLKRDKGWVGMLLEQYLGAMAGSKPEQDFPELGIELKT
IPIDAAGKPLETTFVCVAPLTGNSGVTWASSHVRHKLARVLWIPVEGERQIPLAQRRIGSPLLWSPNAEEEEMLRRDWEE
LMDLIVLGHVERITARHGEVLQLRPKAANSKALTEAIGEQGQPILTLPRGFYLKKSFTGALLARHFFV

Sequences:

>Translated_228_residues
MAFLSPLPPPPENEQQLFERAQQLAGCSLGELAEGARLAIPRDLKRDKGWVGMLLEQYLGAMAGSKPEQDFPELGIELKT
IPIDAAGKPLETTFVCVAPLTGNSGVTWASSHVRHKLARVLWIPVEGERQIPLAQRRIGSPLLWSPNAEEEEMLRRDWEE
LMDLIVLGHVERITARHGEVLQLRPKAANSKALTEAIGEQGQPILTLPRGFYLKKSFTGALLARHFFV
>Mature_227_residues
AFLSPLPPPPENEQQLFERAQQLAGCSLGELAEGARLAIPRDLKRDKGWVGMLLEQYLGAMAGSKPEQDFPELGIELKTI
PIDAAGKPLETTFVCVAPLTGNSGVTWASSHVRHKLARVLWIPVEGERQIPLAQRRIGSPLLWSPNAEEEEMLRRDWEEL
MDLIVLGHVERITARHGEVLQLRPKAANSKALTEAIGEQGQPILTLPRGFYLKKSFTGALLARHFFV

Specific function: Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair

COG id: COG3066

COG function: function code L; DNA mismatch repair protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mutH family

Homologues:

Organism=Escherichia coli, GI1789196, Length=223, Percent_Identity=76.2331838565022, Blast_Score=352, Evalue=1e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MUTH_SERP5 (A8GII1)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480049.1
- ProteinModelPortal:   A8GII1
- SMR:   A8GII1
- STRING:   A8GII1
- GeneID:   5606722
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3825
- eggNOG:   COG3066
- HOGENOM:   HBG296591
- OMA:   QDWEELM
- ProtClustDB:   PRK05070
- BioCyc:   SPRO399741:SPRO_3825-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00759
- InterPro:   IPR004230
- InterPro:   IPR011337
- InterPro:   IPR011335
- Gene3D:   G3DSA:3.40.600.10
- SMART:   SM00927
- TIGRFAMs:   TIGR02248

Pfam domain/function: PF02976 MutH; SSF52980 Restrict_endonuc_II-like_core

EC number: NA

Molecular weight: Translated: 25312; Mature: 25180

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFLSPLPPPPENEQQLFERAQQLAGCSLGELAEGARLAIPRDLKRDKGWVGMLLEQYLG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCEECCCCHHHHCCCHHHHHHHHHHH
AMAGSKPEQDFPELGIELKTIPIDAAGKPLETTFVCVAPLTGNSGVTWASSHVRHKLARV
HHCCCCCCCCHHHHCCEEEEEECCCCCCCCCEEEEEEEEEECCCCCEEHHHHHHHHHHHE
LWIPVEGERQIPLAQRRIGSPLLWSPNAEEEEMLRRDWEELMDLIVLGHVERITARHGEV
EEEECCCCCCCCHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LQLRPKAANSKALTEAIGEQGQPILTLPRGFYLKKSFTGALLARHFFV
EEECCCCCCHHHHHHHHHCCCCEEEECCCCEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AFLSPLPPPPENEQQLFERAQQLAGCSLGELAEGARLAIPRDLKRDKGWVGMLLEQYLG
CCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCEECCCCHHHHCCCHHHHHHHHHHH
AMAGSKPEQDFPELGIELKTIPIDAAGKPLETTFVCVAPLTGNSGVTWASSHVRHKLARV
HHCCCCCCCCHHHHCCEEEEEECCCCCCCCCEEEEEEEEEECCCCCEEHHHHHHHHHHHE
LWIPVEGERQIPLAQRRIGSPLLWSPNAEEEEMLRRDWEELMDLIVLGHVERITARHGEV
EEEECCCCCCCCHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LQLRPKAANSKALTEAIGEQGQPILTLPRGFYLKKSFTGALLARHFFV
EEECCCCCCHHHHHHHHHCCCCEEEECCCCEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA