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The map label for this gene is citA [H]

Identifier: 157371966

GI number: 157371966

Start: 4119357

End: 4120955

Strand: Direct

Name: citA [H]

Synonym: Spro_3731

Alternate gene names: 157371966

Gene position: 4119357-4120955 (Clockwise)

Preceding gene: 157371963

Following gene: 157371967

Centisome position: 75.6

GC content: 53.22

Gene sequence:

>1599_bases
ATGCGCATAAAACTCTCATTTCAAATCAAGCTATTCCTGTGCCTGGTCGCCTTCTCCTGCCTGCTGCTGAGCTTTATTGG
CGCCTATACCTACTATCAGCTCGATGCCCAGTTGCACCGCGATCTCGGCGCGCGAGCGCAAGTACAGGCGCGAGAGATTG
CCCTGATCCCCTCGCTGGTCAGCGCGGTGGAAAAAAACGACCCGGTAAAAATCGCCGAATTGATGAAAAAAATACGCGCC
AGCAGCGATGCCAGCTATATCGTGATCGGCGATAAACAAACCCGGCATCTTTACCACTCTGAATATACCAACCGTGTCGG
TACCCCAATGGTCGGGGGCGATAATAAAGAGGTTTTGGAAGGGAAAAGTATTATTTCCATCCGCAAAGGTGGCATCGGCA
TTTCATTGCGCAGTAAAGCGCCAATTATGGATGAGCATAATAACGTTATCGGCATCGTTTCGGTCGGCTATCTGAAATCT
CATATCGATAACCTGAATGCCAGAACCCTGACGCAAATCGTCGGATCCATTGTGTTGTTATTAATCGCCCTGTTTGTTTT
TTCCTGGCTGTTGTCGAAGAATTTAAAACGCCAGATGTTCTGGCTGGAACCCAAAGAAATTGCGCTACTGGTGCGTCAGC
AAAAAGCCTTGCTGGAGGCCATCTATGAAGGCGTCATCGCCGTCGACCCGCAACTGCGGATCATCACCATCAACCACGCG
GCGCGTGAACTGCTGGAGCTGCGCCAGCCTGCCGCCGCGCTGATGGGCCGCAATATCGACGAGATTATCCAGTCACAGCC
GGATTTTTTCGGCGCTTCCCAGTTGGGGCACGACACTCACGACGAGGTTTGTCGCTTTAACCACGTACGGGTTATCGCCA
GCCGAGTGCGTATTATGCAGGAGCAAGAGCTGCAGGGCTGGGTGATCAGCTTCCGCGACAAGAACGACATCAATACCCTG
AGTAGTCAGCTCAGTCAGGTCAAACGCTACGCTGACAACCTGCGCATCATGCGCCATGAGCAGCTTAACTGGACAGCCAC
CCTGGCCGGGCTGTTGCATATGCAGCGCTACGACGAGGCGATCCGCTATGTGGAGGCTCAATCGGAAGGCGCGCAAGAAA
TTCTCGACTTTATTTCGCAGCGTTTCAGCTCGGCGGCACTTTGTGGTTTGCTGTTGGGCAAGTACTCCAGTGCGAAGGAA
AAAGGCGTTGAGCTGCGCTTCGATCCCGCCTGCCAGCTACGACAAATTCCGGCGGCGCTGAATGAAACCGAACTGATGTC
GATCATCGGCAATTTGCTGGATAATGCGATAGACGCTACGCTGCATTGCGCCGAGCCACACGAGGCGGTAGAACTCTATA
TTAGCGATATCAGCAATGAGCTGGTGATTGAAGTGGCCGATCGCGGTATCGGCATTGCGCCCGAGGTGCGCGATACGCTG
TTCGAGCAAGGCGTCACCACCAAAGAAGATAAAGGTGACCACGGCATCGGCCTGCATTTGGTCGCCAGCCACGTAGCGCA
GGCGCATGGCAGTATAGAAGTGTCGGACAACGAGCCGCACGGGGCCATTTTTTCTTTATTTATCCCTAAAACCTTATAA

Upstream 100 bases:

>100_bases
ACCCGTGGCGGCGATCACAAGTTGATCGGGCGCAATATTACATGAACCCCGACTCATGATAGGGTAAGCGCTCAGGATTT
CTCAGGGTGCCTCCCAGACC

Downstream 100 bases:

>100_bases
CCCGTTTCGAGCACCCGAATGATGCAACCAGAAGCACTGGACGTTTTGATCGTTGAAGATGAGCCGCAGTTGGCCACGCT
GCACGCCGAGTTTATCGAGC

Product: signal transduction histidine kinase regulating citrate/malate metabolism

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MRIKLSFQIKLFLCLVAFSCLLLSFIGAYTYYQLDAQLHRDLGARAQVQAREIALIPSLVSAVEKNDPVKIAELMKKIRA
SSDASYIVIGDKQTRHLYHSEYTNRVGTPMVGGDNKEVLEGKSIISIRKGGIGISLRSKAPIMDEHNNVIGIVSVGYLKS
HIDNLNARTLTQIVGSIVLLLIALFVFSWLLSKNLKRQMFWLEPKEIALLVRQQKALLEAIYEGVIAVDPQLRIITINHA
ARELLELRQPAAALMGRNIDEIIQSQPDFFGASQLGHDTHDEVCRFNHVRVIASRVRIMQEQELQGWVISFRDKNDINTL
SSQLSQVKRYADNLRIMRHEQLNWTATLAGLLHMQRYDEAIRYVEAQSEGAQEILDFISQRFSSAALCGLLLGKYSSAKE
KGVELRFDPACQLRQIPAALNETELMSIIGNLLDNAIDATLHCAEPHEAVELYISDISNELVIEVADRGIGIAPEVRDTL
FEQGVTTKEDKGDHGIGLHLVASHVAQAHGSIEVSDNEPHGAIFSLFIPKTL

Sequences:

>Translated_532_residues
MRIKLSFQIKLFLCLVAFSCLLLSFIGAYTYYQLDAQLHRDLGARAQVQAREIALIPSLVSAVEKNDPVKIAELMKKIRA
SSDASYIVIGDKQTRHLYHSEYTNRVGTPMVGGDNKEVLEGKSIISIRKGGIGISLRSKAPIMDEHNNVIGIVSVGYLKS
HIDNLNARTLTQIVGSIVLLLIALFVFSWLLSKNLKRQMFWLEPKEIALLVRQQKALLEAIYEGVIAVDPQLRIITINHA
ARELLELRQPAAALMGRNIDEIIQSQPDFFGASQLGHDTHDEVCRFNHVRVIASRVRIMQEQELQGWVISFRDKNDINTL
SSQLSQVKRYADNLRIMRHEQLNWTATLAGLLHMQRYDEAIRYVEAQSEGAQEILDFISQRFSSAALCGLLLGKYSSAKE
KGVELRFDPACQLRQIPAALNETELMSIIGNLLDNAIDATLHCAEPHEAVELYISDISNELVIEVADRGIGIAPEVRDTL
FEQGVTTKEDKGDHGIGLHLVASHVAQAHGSIEVSDNEPHGAIFSLFIPKTL
>Mature_532_residues
MRIKLSFQIKLFLCLVAFSCLLLSFIGAYTYYQLDAQLHRDLGARAQVQAREIALIPSLVSAVEKNDPVKIAELMKKIRA
SSDASYIVIGDKQTRHLYHSEYTNRVGTPMVGGDNKEVLEGKSIISIRKGGIGISLRSKAPIMDEHNNVIGIVSVGYLKS
HIDNLNARTLTQIVGSIVLLLIALFVFSWLLSKNLKRQMFWLEPKEIALLVRQQKALLEAIYEGVIAVDPQLRIITINHA
ARELLELRQPAAALMGRNIDEIIQSQPDFFGASQLGHDTHDEVCRFNHVRVIASRVRIMQEQELQGWVISFRDKNDINTL
SSQLSQVKRYADNLRIMRHEQLNWTATLAGLLHMQRYDEAIRYVEAQSEGAQEILDFISQRFSSAALCGLLLGKYSSAKE
KGVELRFDPACQLRQIPAALNETELMSIIGNLLDNAIDATLHCAEPHEAVELYISDISNELVIEVADRGIGIAPEVRDTL
FEQGVTTKEDKGDHGIGLHLVASHVAQAHGSIEVSDNEPHGAIFSLFIPKTL

Specific function: Member of the two-component regulatory system CitA/CitB. Probably activates CitB by phosphorylation. The periplasmic domain binds H-citrate(2-), which is essential for induction of the citrate-fermentation genes [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=529, Percent_Identity=40.6427221172023, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1790567, Length=530, Percent_Identity=29.4339622641509, Blast_Score=199, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59395; Mature: 59395

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIKLSFQIKLFLCLVAFSCLLLSFIGAYTYYQLDAQLHRDLGARAQVQAREIALIPSLV
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
SAVEKNDPVKIAELMKKIRASSDASYIVIGDKQTRHLYHSEYTNRVGTPMVGGDNKEVLE
HHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHC
GKSIISIRKGGIGISLRSKAPIMDEHNNVIGIVSVGYLKSHIDNLNARTLTQIVGSIVLL
CCCEEEEECCCEEEEECCCCCCCCCCCCEEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHH
LIALFVFSWLLSKNLKRQMFWLEPKEIALLVRQQKALLEAIYEGVIAVDPQLRIITINHA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEEECHH
ARELLELRQPAAALMGRNIDEIIQSQPDFFGASQLGHDTHDEVCRFNHVRVIASRVRIMQ
HHHHHHHHCHHHHHHCCCHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
EQELQGWVISFRDKNDINTLSSQLSQVKRYADNLRIMRHEQLNWTATLAGLLHMQRYDEA
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IRYVEAQSEGAQEILDFISQRFSSAALCGLLLGKYSSAKEKGVELRFDPACQLRQIPAAL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEECCCHHHHHHHHHC
NETELMSIIGNLLDNAIDATLHCAEPHEAVELYISDISNELVIEVADRGIGIAPEVRDTL
CHHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
FEQGVTTKEDKGDHGIGLHLVASHVAQAHGSIEVSDNEPHGAIFSLFIPKTL
HHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MRIKLSFQIKLFLCLVAFSCLLLSFIGAYTYYQLDAQLHRDLGARAQVQAREIALIPSLV
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
SAVEKNDPVKIAELMKKIRASSDASYIVIGDKQTRHLYHSEYTNRVGTPMVGGDNKEVLE
HHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHC
GKSIISIRKGGIGISLRSKAPIMDEHNNVIGIVSVGYLKSHIDNLNARTLTQIVGSIVLL
CCCEEEEECCCEEEEECCCCCCCCCCCCEEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHH
LIALFVFSWLLSKNLKRQMFWLEPKEIALLVRQQKALLEAIYEGVIAVDPQLRIITINHA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEEECHH
ARELLELRQPAAALMGRNIDEIIQSQPDFFGASQLGHDTHDEVCRFNHVRVIASRVRIMQ
HHHHHHHHCHHHHHHCCCHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
EQELQGWVISFRDKNDINTLSSQLSQVKRYADNLRIMRHEQLNWTATLAGLLHMQRYDEA
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IRYVEAQSEGAQEILDFISQRFSSAALCGLLLGKYSSAKEKGVELRFDPACQLRQIPAAL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEECCCHHHHHHHHHC
NETELMSIIGNLLDNAIDATLHCAEPHEAVELYISDISNELVIEVADRGIGIAPEVRDTL
CHHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
FEQGVTTKEDKGDHGIGLHLVASHVAQAHGSIEVSDNEPHGAIFSLFIPKTL
HHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8748036; 10447894 [H]