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The map label for this gene is citB [H]

Identifier: 157371967

GI number: 157371967

Start: 4120974

End: 4121663

Strand: Direct

Name: citB [H]

Synonym: Spro_3732

Alternate gene names: 157371967

Gene position: 4120974-4121663 (Clockwise)

Preceding gene: 157371966

Following gene: 157371968

Centisome position: 75.63

GC content: 55.07

Gene sequence:

>690_bases
ATGATGCAACCAGAAGCACTGGACGTTTTGATCGTTGAAGATGAGCCGCAGTTGGCCACGCTGCACGCCGAGTTTATCGA
GCAAAACTTTGCTCTGCGGGTGGTGGCTTATGCCGCTACGCTGACCGAGGCCAGAGCCAAGGTCAACGCCCACCAGCCGC
GGTTGATCCTGCTGGATAACTTTTTGCCGGACGGCCAGGGTATCGAGCTGATGGAAGAAGCTGCGGTGAAAAACCCCGGC
TGTTCGGTGATATTCATTACCGCCGCCAGCGATATGAACACCTGCAGCCAGGCGATCCGCAACGGTGCCTTCGACTACAT
CATCAAACCGGTATCCTATAAGCGGCTGCGTAACTCGCTGGAGCGTTTTATGCAGTTTGTTCAAACCCAGCGCACCTTTA
AGGTGATCGACCAAAGCAACGTCGACGCACTGTATAACCTGCAATCCAAACAGTTCTCCAGCGAACCCAGTGCCAAGGGC
ATTGAAACCAATACGCTGGAGTTGGTGCAGGCGCTGTTTATCGAACAGCCGGAGGCGGCGCACGCGGTGGAAGACGTGGT
CAAACAGGTGGGTATCAGCAAAACCACCGCCCGGCGTTATCTGGAATATTGCGTCGCTACCCAGTTCGTGCGCGTAGAGA
TGATGTACGGCAATATCGGTCATCCACGCCGTCTGTATCGCAAGGCTTAA

Upstream 100 bases:

>100_bases
GCAGGCGCATGGCAGTATAGAAGTGTCGGACAACGAGCCGCACGGGGCCATTTTTTCTTTATTTATCCCTAAAACCTTAT
AACCCGTTTCGAGCACCCGA

Downstream 100 bases:

>100_bases
TCTGACATCTGGCTGTCATATTAAGCGGATAATATGACTGCGACGATATCCCCTCGATATCGGCACCTGTCGTTGAAATT
AATGAAATACTGATGACTTT

Product: response regulator receiver/unknown domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MMQPEALDVLIVEDEPQLATLHAEFIEQNFALRVVAYAATLTEARAKVNAHQPRLILLDNFLPDGQGIELMEEAAVKNPG
CSVIFITAASDMNTCSQAIRNGAFDYIIKPVSYKRLRNSLERFMQFVQTQRTFKVIDQSNVDALYNLQSKQFSSEPSAKG
IETNTLELVQALFIEQPEAAHAVEDVVKQVGISKTTARRYLEYCVATQFVRVEMMYGNIGHPRRLYRKA

Sequences:

>Translated_229_residues
MMQPEALDVLIVEDEPQLATLHAEFIEQNFALRVVAYAATLTEARAKVNAHQPRLILLDNFLPDGQGIELMEEAAVKNPG
CSVIFITAASDMNTCSQAIRNGAFDYIIKPVSYKRLRNSLERFMQFVQTQRTFKVIDQSNVDALYNLQSKQFSSEPSAKG
IETNTLELVQALFIEQPEAAHAVEDVVKQVGISKTTARRYLEYCVATQFVRVEMMYGNIGHPRRLYRKA
>Mature_229_residues
MMQPEALDVLIVEDEPQLATLHAEFIEQNFALRVVAYAATLTEARAKVNAHQPRLILLDNFLPDGQGIELMEEAAVKNPG
CSVIFITAASDMNTCSQAIRNGAFDYIIKPVSYKRLRNSLERFMQFVQTQRTFKVIDQSNVDALYNLQSKQFSSEPSAKG
IETNTLELVQALFIEQPEAAHAVEDVVKQVGISKTTARRYLEYCVATQFVRVEMMYGNIGHPRRLYRKA

Specific function: Member of the two-component regulatory system CitA/CitB essential for expression of citrate-specific fermentation genes. Phosphorylated CitB binds to two sites in the citS-citC intergenic region where it probably activates transcription of both genes [H]

COG id: COG4565

COG function: function code KT; Response regulator of citrate/malate metabolism

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786838, Length=223, Percent_Identity=40.3587443946188, Blast_Score=179, Evalue=1e-46,
Organism=Escherichia coli, GI1790566, Length=226, Percent_Identity=26.1061946902655, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 25862; Mature: 25862

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQPEALDVLIVEDEPQLATLHAEFIEQNFALRVVAYAATLTEARAKVNAHQPRLILLDN
CCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
FLPDGQGIELMEEAAVKNPGCSVIFITAASDMNTCSQAIRNGAFDYIIKPVSYKRLRNSL
CCCCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHCCCCHHHHHHHHH
ERFMQFVQTQRTFKVIDQSNVDALYNLQSKQFSSEPSAKGIETNTLELVQALFIEQPEAA
HHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHH
HAVEDVVKQVGISKTTARRYLEYCVATQFVRVEMMYGNIGHPRRLYRKA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHCC
>Mature Secondary Structure
MMQPEALDVLIVEDEPQLATLHAEFIEQNFALRVVAYAATLTEARAKVNAHQPRLILLDN
CCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
FLPDGQGIELMEEAAVKNPGCSVIFITAASDMNTCSQAIRNGAFDYIIKPVSYKRLRNSL
CCCCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHCCCCHHHHHHHHH
ERFMQFVQTQRTFKVIDQSNVDALYNLQSKQFSSEPSAKGIETNTLELVQALFIEQPEAA
HHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHH
HAVEDVVKQVGISKTTARRYLEYCVATQFVRVEMMYGNIGHPRRLYRKA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8748036; 9223636; 10447894 [H]