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The map label for this gene is mltF [H]

Identifier: 157371890

GI number: 157371890

Start: 4047781

End: 4049262

Strand: Direct

Name: mltF [H]

Synonym: Spro_3655

Alternate gene names: 157371890

Gene position: 4047781-4049262 (Clockwise)

Preceding gene: 157371874

Following gene: 157371892

Centisome position: 74.29

GC content: 56.07

Gene sequence:

>1482_bases
GTGCCACCACTGGATAACTATTTGAAACGCCTTAAAATAAACTACATTCTAATCGGTGTTGTTACCTTGCTTCTGGCGCT
CGCCCTGTGGCCCAATATTACCTGGCGCGGCGGTCAGGGCGGGCAACTCGAAGAGATCAAATCGCGCGGAGAGTTGCGTA
TCAGTACGCTAAACTCGCCGTTGACCTATTTCACCACCAAGCAGGGGCCGAGTGGTCTCGACTACGAGCTGGCAAAACGC
TTCGCCAACTACCTGGGCGTGAAACTGGTGGTGATCCCGCACAAGAACATTAATGACCTGTTTGACGATTTGGACGATGA
CGATGCCGACCTGTTGGCCGCCGGCCTGATCTACAATCAGGATCGCCTCAGCCGTGCGCGCACCGGCCCGGCTTATTATT
CCGTTTCCCAACAGTTGGTTTATCGCCTGGGTACTGCCCGACCGAAAACCTTTGCTGATATTAAAGGCAAACTGGCGGTG
GCCTCCGGCTCGGCACACGTCAGCACCCTGAAACAGCTCAAGCAAAGTAAATTCCCGGATCTCAGTTGGGAAGCCTCCAG
CGACCTCACCTCCAAAGAGCTGCTCGAGCAGGTGGCCGACGGCAAGCTGGATTACACCCTCGGCGATTCCGTTACCATCG
CGCTGTTGCAACGCATCCACCCGCAATTGGCAGTGGCGTTCGACGTCACCGATGAAGAACCGGTCACCTGGTATCTGAAA
CGTGGCACCGATGACAGCCTGTATGCGGCAATGCTGGACTTCTACAGCCAGATGGTTGATGACGGCACGCTGGCGCGGCT
GGAAGAGAAGTACCTCGGCCACGTAGGCAGTTTCGATTACGTCGATACCAAAACCTTCCTGTCCGCCATCGATTCGGTGC
TGCCGACTTTCCGTTCGCTATTTGAAAAGTACGCCAGCGAGATCGACTGGAAACTGCTGGCGGCCATTGCCTATCAGGAA
TCGCACTGGAACCCGCAGGCAACCTCACCGACCGGCGTGCGTGGCCTGATGATGCTGACACGAGCCACCGCCGACGGTCT
GGGGGTTAACGATCGTCTCGATCCCGAGGAAAGTATTCAGGGCGGTGCCCTCTACCTGCAAAGGCTAATGGCCAAGGTGC
CCGACAGCGTGCCGGAAGATGAACGTATCTGGTTCTCACTGGCAGCCTATAACATGGGCTGGGGCCATATGCTCGATGCC
CGCAAACTGACCAAAATGCAAAAAGGCAATCCGGACAGTTGGGTCGATGTCAAACAGCGTCTGCCGATGCTCAGCCAGAA
ACGCTACTACCCGCAATTAACCTACGGCTATGCGCGTGGGCGCGAAGCCTATAACTACGTGGAAAACATTCGCCGCTATC
AGGTCAGCCTGGTGGGTTATTTGCAGGAAAAAGAGAGAAAGGCGGCACAGGAAGCGGCCGAACAGGCTGACCAGGGGAAA
GGTTATCCGGCCGTTACGCCGGAACTGGCACTGAATTTCTGA

Upstream 100 bases:

>100_bases
GTGTCAATAAAGTTGCGCGCTGGCGTTTTGTTGCGCAAAATGCCCCGGCACTGTTGATTTAACCATAAGAAAAGACTGGT
CAGCCGTCGGCAGTGCATGT

Downstream 100 bases:

>100_bases
TTATTCCGGCTTGTTATCGACATCGGTACGCTGCGCTTGTTTCAACGCCTTTTGCTGCTCACGACGCATGCGGAAAAAGT
TGCTCAGCGTAGCGGCGCAC

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MPPLDNYLKRLKINYILIGVVTLLLALALWPNITWRGGQGGQLEEIKSRGELRISTLNSPLTYFTTKQGPSGLDYELAKR
FANYLGVKLVVIPHKNINDLFDDLDDDDADLLAAGLIYNQDRLSRARTGPAYYSVSQQLVYRLGTARPKTFADIKGKLAV
ASGSAHVSTLKQLKQSKFPDLSWEASSDLTSKELLEQVADGKLDYTLGDSVTIALLQRIHPQLAVAFDVTDEEPVTWYLK
RGTDDSLYAAMLDFYSQMVDDGTLARLEEKYLGHVGSFDYVDTKTFLSAIDSVLPTFRSLFEKYASEIDWKLLAAIAYQE
SHWNPQATSPTGVRGLMMLTRATADGLGVNDRLDPEESIQGGALYLQRLMAKVPDSVPEDERIWFSLAAYNMGWGHMLDA
RKLTKMQKGNPDSWVDVKQRLPMLSQKRYYPQLTYGYARGREAYNYVENIRRYQVSLVGYLQEKERKAAQEAAEQADQGK
GYPAVTPELALNF

Sequences:

>Translated_493_residues
MPPLDNYLKRLKINYILIGVVTLLLALALWPNITWRGGQGGQLEEIKSRGELRISTLNSPLTYFTTKQGPSGLDYELAKR
FANYLGVKLVVIPHKNINDLFDDLDDDDADLLAAGLIYNQDRLSRARTGPAYYSVSQQLVYRLGTARPKTFADIKGKLAV
ASGSAHVSTLKQLKQSKFPDLSWEASSDLTSKELLEQVADGKLDYTLGDSVTIALLQRIHPQLAVAFDVTDEEPVTWYLK
RGTDDSLYAAMLDFYSQMVDDGTLARLEEKYLGHVGSFDYVDTKTFLSAIDSVLPTFRSLFEKYASEIDWKLLAAIAYQE
SHWNPQATSPTGVRGLMMLTRATADGLGVNDRLDPEESIQGGALYLQRLMAKVPDSVPEDERIWFSLAAYNMGWGHMLDA
RKLTKMQKGNPDSWVDVKQRLPMLSQKRYYPQLTYGYARGREAYNYVENIRRYQVSLVGYLQEKERKAAQEAAEQADQGK
GYPAVTPELALNF
>Mature_492_residues
PPLDNYLKRLKINYILIGVVTLLLALALWPNITWRGGQGGQLEEIKSRGELRISTLNSPLTYFTTKQGPSGLDYELAKRF
ANYLGVKLVVIPHKNINDLFDDLDDDDADLLAAGLIYNQDRLSRARTGPAYYSVSQQLVYRLGTARPKTFADIKGKLAVA
SGSAHVSTLKQLKQSKFPDLSWEASSDLTSKELLEQVADGKLDYTLGDSVTIALLQRIHPQLAVAFDVTDEEPVTWYLKR
GTDDSLYAAMLDFYSQMVDDGTLARLEEKYLGHVGSFDYVDTKTFLSAIDSVLPTFRSLFEKYASEIDWKLLAAIAYQES
HWNPQATSPTGVRGLMMLTRATADGLGVNDRLDPEESIQGGALYLQRLMAKVPDSVPEDERIWFSLAAYNMGWGHMLDAR
KLTKMQKGNPDSWVDVKQRLPMLSQKRYYPQLTYGYARGREAYNYVENIRRYQVSLVGYLQEKERKAAQEAAEQADQGKG
YPAVTPELALNF

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=481, Percent_Identity=62.5779625779626, Blast_Score=620, Evalue=1e-179,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 55448; Mature: 55316

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPLDNYLKRLKINYILIGVVTLLLALALWPNITWRGGQGGQLEEIKSRGELRISTLNSP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHCCCCEEEEECCCC
LTYFTTKQGPSGLDYELAKRFANYLGVKLVVIPHKNINDLFDDLDDDDADLLAAGLIYNQ
EEEEEECCCCCCCCHHHHHHHHHHHCEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHCCH
DRLSRARTGPAYYSVSQQLVYRLGTARPKTFADIKGKLAVASGSAHVSTLKQLKQSKFPD
HHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEECCCHHHHHHHHHHHCCCCC
LSWEASSDLTSKELLEQVADGKLDYTLGDSVTIALLQRIHPQLAVAFDVTDEEPVTWYLK
CCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEEE
RGTDDSLYAAMLDFYSQMVDDGTLARLEEKYLGHVGSFDYVDTKTFLSAIDSVLPTFRSL
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FEKYASEIDWKLLAAIAYQESHWNPQATSPTGVRGLMMLTRATADGLGVNDRLDPEESIQ
HHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHCC
GGALYLQRLMAKVPDSVPEDERIWFSLAAYNMGWGHMLDARKLTKMQKGNPDSWVDVKQR
HHHHHHHHHHHHCCCCCCCCCCEEEEEEHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
LPMLSQKRYYPQLTYGYARGREAYNYVENIRRYQVSLVGYLQEKERKAAQEAAEQADQGK
CCHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GYPAVTPELALNF
CCCCCCHHHHCCC
>Mature Secondary Structure 
PPLDNYLKRLKINYILIGVVTLLLALALWPNITWRGGQGGQLEEIKSRGELRISTLNSP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHCCCCEEEEECCCC
LTYFTTKQGPSGLDYELAKRFANYLGVKLVVIPHKNINDLFDDLDDDDADLLAAGLIYNQ
EEEEEECCCCCCCCHHHHHHHHHHHCEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHCCH
DRLSRARTGPAYYSVSQQLVYRLGTARPKTFADIKGKLAVASGSAHVSTLKQLKQSKFPD
HHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEECCCHHHHHHHHHHHCCCCC
LSWEASSDLTSKELLEQVADGKLDYTLGDSVTIALLQRIHPQLAVAFDVTDEEPVTWYLK
CCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEEE
RGTDDSLYAAMLDFYSQMVDDGTLARLEEKYLGHVGSFDYVDTKTFLSAIDSVLPTFRSL
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FEKYASEIDWKLLAAIAYQESHWNPQATSPTGVRGLMMLTRATADGLGVNDRLDPEESIQ
HHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHCC
GGALYLQRLMAKVPDSVPEDERIWFSLAAYNMGWGHMLDARKLTKMQKGNPDSWVDVKQR
HHHHHHHHHHHHCCCCCCCCCCEEEEEEHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
LPMLSQKRYYPQLTYGYARGREAYNYVENIRRYQVSLVGYLQEKERKAAQEAAEQADQGK
CCHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GYPAVTPELALNF
CCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA