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The map label for this gene is hmp [H]

Identifier: 157371874

GI number: 157371874

Start: 4024172

End: 4025362

Strand: Direct

Name: hmp [H]

Synonym: Spro_3639

Alternate gene names: 157371874

Gene position: 4024172-4025362 (Clockwise)

Preceding gene: 157371868

Following gene: 157371890

Centisome position: 73.85

GC content: 57.6

Gene sequence:

>1191_bases
ATGCTGGATAGCCAAACCATCGCCACCGTAAAATCTACCATTCCCCTGCTGGCCGCTACCGGGCCAAAACTGACCGCCCA
CTTCTACGATCGCATGTTCGAGCATAATCCCGAGTTAAAGGACGTGTTCAATATGAACAACCAGCGCAATGGCGATCAGC
GTCAGGCGCTGTTCGACGCGATCTGCGCCTATGCCGCGAATATCGAAAACCTGGCGGCGCTGCTGCCGGCGGTGGAGCGC
ATTGCCCAGAAGCATACCAGCCTGAATATTCGCCCGGATCAATATCAGATTGTCGGTGGTCATCTGCTGGCAACTCTGGA
CGAAATGTTCAGCCCGGGGCAGGAAGTGCTGGACGCCTGGGGTAAAGCCTACGGGGTGTTGGCGGATGTATTTATCCAGC
GCGAAGGCCAGATTTATCAGGCCAGCGAAACTGCCGAGGGTGGCTGGCGCGATCGACGAGCCTTCCGCATCGTCAAAAAA
CAGCCACAGAGCGAGGTGATTTGCAGCTTCGTGTTGGCGCCGGTTGACGGTGGCAAGGTGATCGACTTCAAACCGGGCCA
ATACCTGGCGGTCTACATCAAGCACGACAGCATGGAAAACCAGGAGATTCGCCAATACTCACTGACCACCTCACCGAATG
GCGAGTACTATCGCATTGCGGTAAAACGAGAGGATCAGGGCAAGGTATCGAACTTCCTGCACCAGCAGGCCAAAGAAGGC
GACGTCATTTATATCGCTCCGCCGCACGGGGATTTCTTCCTGAAAGCGCAGCCGGAAACCCCGGTGGCACTGATCTCCGC
CGGTGTCGGCCAAACACCAATGCTCGGCATGCTCCATACGCTGCACGATAACCAGCACCCGGCGGACGTGCACTGGCTGC
ACGCGGCGGAAAACGGCAGCGTTCACGCCTTTGCCGATGAAGTAGCGGATATTGCCGGCCGCATGCCGAATCTGAGCCGT
CACGTGTGGTACAACCAACCGGGTGCCAATGATGTGGAAGGCCGCGACTACCAGAGCCGTGGCCTGATGAATCTCAGTGC
CCTGCGTGAAACGCTCAGCACGCCGCAGATGCATTACTACTTCTGCGGGCCGTTGCCGTTTATGCAGTCTGTGGCCAGCC
AACTTCTGGACATGGGCGTAGAGGCCGAACGCATCCACTACGAATGCTTCGGGCCGCACAAGGTCATGTAA

Upstream 100 bases:

>100_bases
ACAGCCACGGCAGAAACCGTTAGGATGCGTTCAAACACAAAGATGCATTTAAAATGCATTATTAGAATATTCCAATAATG
TCCGACCAAGGAGCGCCACC

Downstream 100 bases:

>100_bases
TTGAAATGGGGCGCGGCCTGCTGCCGTGCCCTCAGACGGCTGATAATGCCCGTTATTAGGGAGAGCGTGCCGAAGGGGTC
GTAGCGGCTTGCCCGCCGGA

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MLDSQTIATVKSTIPLLAATGPKLTAHFYDRMFEHNPELKDVFNMNNQRNGDQRQALFDAICAYAANIENLAALLPAVER
IAQKHTSLNIRPDQYQIVGGHLLATLDEMFSPGQEVLDAWGKAYGVLADVFIQREGQIYQASETAEGGWRDRRAFRIVKK
QPQSEVICSFVLAPVDGGKVIDFKPGQYLAVYIKHDSMENQEIRQYSLTTSPNGEYYRIAVKREDQGKVSNFLHQQAKEG
DVIYIAPPHGDFFLKAQPETPVALISAGVGQTPMLGMLHTLHDNQHPADVHWLHAAENGSVHAFADEVADIAGRMPNLSR
HVWYNQPGANDVEGRDYQSRGLMNLSALRETLSTPQMHYYFCGPLPFMQSVASQLLDMGVEAERIHYECFGPHKVM

Sequences:

>Translated_396_residues
MLDSQTIATVKSTIPLLAATGPKLTAHFYDRMFEHNPELKDVFNMNNQRNGDQRQALFDAICAYAANIENLAALLPAVER
IAQKHTSLNIRPDQYQIVGGHLLATLDEMFSPGQEVLDAWGKAYGVLADVFIQREGQIYQASETAEGGWRDRRAFRIVKK
QPQSEVICSFVLAPVDGGKVIDFKPGQYLAVYIKHDSMENQEIRQYSLTTSPNGEYYRIAVKREDQGKVSNFLHQQAKEG
DVIYIAPPHGDFFLKAQPETPVALISAGVGQTPMLGMLHTLHDNQHPADVHWLHAAENGSVHAFADEVADIAGRMPNLSR
HVWYNQPGANDVEGRDYQSRGLMNLSALRETLSTPQMHYYFCGPLPFMQSVASQLLDMGVEAERIHYECFGPHKVM
>Mature_396_residues
MLDSQTIATVKSTIPLLAATGPKLTAHFYDRMFEHNPELKDVFNMNNQRNGDQRQALFDAICAYAANIENLAALLPAVER
IAQKHTSLNIRPDQYQIVGGHLLATLDEMFSPGQEVLDAWGKAYGVLADVFIQREGQIYQASETAEGGWRDRRAFRIVKK
QPQSEVICSFVLAPVDGGKVIDFKPGQYLAVYIKHDSMENQEIRQYSLTTSPNGEYYRIAVKREDQGKVSNFLHQQAKEG
DVIYIAPPHGDFFLKAQPETPVALISAGVGQTPMLGMLHTLHDNQHPADVHWLHAAENGSVHAFADEVADIAGRMPNLSR
HVWYNQPGANDVEGRDYQSRGLMNLSALRETLSTPQMHYYFCGPLPFMQSVASQLLDMGVEAERIHYECFGPHKVM

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=396, Percent_Identity=64.6464646464647, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1787098, Length=203, Percent_Identity=26.6009852216749, Blast_Score=76, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6321673, Length=410, Percent_Identity=32.1951219512195, Blast_Score=200, Evalue=3e-52,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 44337; Mature: 44337

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDSQTIATVKSTIPLLAATGPKLTAHFYDRMFEHNPELKDVFNMNNQRNGDQRQALFDA
CCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHH
ICAYAANIENLAALLPAVERIAQKHTSLNIRPDQYQIVGGHLLATLDEMFSPGQEVLDAW
HHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHCCHHHHHHHH
GKAYGVLADVFIQREGQIYQASETAEGGWRDRRAFRIVKKQPQSEVICSFVLAPVDGGKV
HHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHEEECCCCEE
IDFKPGQYLAVYIKHDSMENQEIRQYSLTTSPNGEYYRIAVKREDQGKVSNFLHQQAKEG
EEECCCCEEEEEEECCCCCCCHHHEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHCCC
DVIYIAPPHGDFFLKAQPETPVALISAGVGQTPMLGMLHTLHDNQHPADVHWLHAAENGS
CEEEEECCCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCC
VHAFADEVADIAGRMPNLSRHVWYNQPGANDVEGRDYQSRGLMNLSALRETLSTPQMHYY
EEHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCEEEE
FCGPLPFMQSVASQLLDMGVEAERIHYECFGPHKVM
EECCHHHHHHHHHHHHHCCCCHHEEEEEECCCCCCC
>Mature Secondary Structure
MLDSQTIATVKSTIPLLAATGPKLTAHFYDRMFEHNPELKDVFNMNNQRNGDQRQALFDA
CCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHH
ICAYAANIENLAALLPAVERIAQKHTSLNIRPDQYQIVGGHLLATLDEMFSPGQEVLDAW
HHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHCCHHHHHHHH
GKAYGVLADVFIQREGQIYQASETAEGGWRDRRAFRIVKKQPQSEVICSFVLAPVDGGKV
HHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHEEECCCCEE
IDFKPGQYLAVYIKHDSMENQEIRQYSLTTSPNGEYYRIAVKREDQGKVSNFLHQQAKEG
EEECCCCEEEEEEECCCCCCCHHHEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHCCC
DVIYIAPPHGDFFLKAQPETPVALISAGVGQTPMLGMLHTLHDNQHPADVHWLHAAENGS
CEEEEECCCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCC
VHAFADEVADIAGRMPNLSRHVWYNQPGANDVEGRDYQSRGLMNLSALRETLSTPQMHYY
EEHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCEEEE
FCGPLPFMQSVASQLLDMGVEAERIHYECFGPHKVM
EECCHHHHHHHHHHHHHCCCCHHEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]