| Definition | Serratia proteamaculans 568 chromosome, complete genome. |
|---|---|
| Accession | NC_009832 |
| Length | 5,448,853 |
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The map label for this gene is nadE [H]
Identifier: 157371885
GI number: 157371885
Start: 4037435
End: 4039057
Strand: Reverse
Name: nadE [H]
Synonym: Spro_3650
Alternate gene names: 157371885
Gene position: 4039057-4037435 (Counterclockwise)
Preceding gene: 157371886
Following gene: 157371884
Centisome position: 74.13
GC content: 57.67
Gene sequence:
>1623_bases ATGAGCAGATCACTTTCCATCGCACTGGCCCAGCTGAATCTGCTGGTCGGTGACATTGAAGGCAACACCGAACGCATGTT GCAAACCGTGCAAGAGCAGCAGAAGGCGGGAGCCGATCTGGTCATGTTCACCGAGTTGGCGCTCTCCGGCTATCCGCCGG AAGACCTGCTGTACCGCAACGATTTTTACCAGCGCTGTGACGCGCAGCTGCAGCGTCTGCAGCAGGCATCTGCCGAGGTC GCCATTCTGGTTGGCCATCCGTGGCGTGAAGGCGACAAGCTGTATAATGCGCTGTCGCTGTTTTCTGAAGGGCAGTTGCT GGCGCGTTACTTCAAGCAGCAGTTGCCGAACTACGGCGTTTTCGATGAGAAACGCTACTTCCAGGCCGGTAGTGAAACCT GCGTGGTTGAGCTGAAAGGCTATCAATTGGGCCTGCTGATTTGCGAAGATCTGTGGTTCCCAGGGCCGGTCGATGCCGCC AAGGCGGCCGGTGCCGAAATGATTTTGTCGATCAACGCCTCGCCGTACAACCGCGAAAAACCGTATATCCGCAAAACGTT AATGGCCGGCCATTGCCAACGTACCCATCTGCCGCTGGTGTATCTGAACCAGATTGGCGGGCAGGATGAGCTGATTTTCG ACGGCTGTTCGAAGGTGTTCGATGCGGCCGGTAACATGACCCATCGCCTGACGGCGTTTGCCGAGCAGGTGACCCTGCTT GAGCTCAATGAGCTGGAGGTGGTGCCAATGACCGCTCCGGCGGCCGAGCTGCCACAGTTGGCGCAGGTGTACGAAGCCCT GGTGTTGGCGGTGCGCGATTACGTCACTAAAAACGGCTTTAAGGGCGCGGTACTGGGGTTGTCTGGCGGTATCGATTCGG CGCTGACGCTGGCGATTGCCGTCGATGCCTTGGGCAAAGATAAAGTGCAGGCGCTGATGATGCCGTTCCGCTACACCGCG GATATCAGTATCGCTGACGCCAAAGAAGAAGCTGAAATCCTCGGCATTGAATTCGACATCGTCTCTATTGAACCTATGTT CGACGCCTTTATGGGCCAGCTGACGCCAATGTTTGCCGGTACCGAACGCGACACCACCGAAGAGAACCTGCAGGCACGCT GCCGCGGCGTGGTGTTGATGGCGCTGTCCAACAAGCGCCGCAGCATCGTGTTGACCACCGGCAACAAAAGCGAGATGGCC GTAGGTTACGCCACGCTGTATGGTGATATGGCAGGTGGCTTCGACGTGCTGAAAGACGTACCGAAAACGCTGGTGTTCAA GCTTTCCGAATACCGTAACACCGTCTCATACGTGATCCCGCAGCGGGTTATCGATCGTCCGCCGTCCGCAGAGCTGGCGC CGGATCAGCTCGATCAGGACAGCCTGCCGCCGTACGACATTCTGGATGCAATTCTGGAAGGTTACGTCGAGCGCGATAAG TCGGTTGCCGATCTGGTGGCCGAAGGCTTCGACGAGGCGATCGTGCGCAAGGTTATCCGTCTGGTGGATATCAACGAATA CAAACGGCGTCAGGCCGCCGTCGGGCCACGCATCACCGCCCGCAATTTCGGCAAAGACCGCCGCTACCCAATCACATCCG GCTTTGGCCGCAAAAATTGGTAA
Upstream 100 bases:
>100_bases TTCTCAACAAATGCGGCGGAATGATTTACACTCCGTTCTCTTGTTTGTGGCGTGGCCCAACACAGAGCACCATGGAACGT ATTAAGCGGGATCCTAAACA
Downstream 100 bases:
>100_bases AGGAAACAAGATGAAAAAGATCGACGCTATTATCAAGCCGTTCAAACTGGATGATGTCCGTGAAGCGCTGGCTGAGGTTG GCATTACCGGGATGACCGTC
Product: NAD synthetase
Products: NA
Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]
Number of amino acids: Translated: 540; Mature: 539
Protein sequence:
>540_residues MSRSLSIALAQLNLLVGDIEGNTERMLQTVQEQQKAGADLVMFTELALSGYPPEDLLYRNDFYQRCDAQLQRLQQASAEV AILVGHPWREGDKLYNALSLFSEGQLLARYFKQQLPNYGVFDEKRYFQAGSETCVVELKGYQLGLLICEDLWFPGPVDAA KAAGAEMILSINASPYNREKPYIRKTLMAGHCQRTHLPLVYLNQIGGQDELIFDGCSKVFDAAGNMTHRLTAFAEQVTLL ELNELEVVPMTAPAAELPQLAQVYEALVLAVRDYVTKNGFKGAVLGLSGGIDSALTLAIAVDALGKDKVQALMMPFRYTA DISIADAKEEAEILGIEFDIVSIEPMFDAFMGQLTPMFAGTERDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMA VGYATLYGDMAGGFDVLKDVPKTLVFKLSEYRNTVSYVIPQRVIDRPPSAELAPDQLDQDSLPPYDILDAILEGYVERDK SVADLVAEGFDEAIVRKVIRLVDINEYKRRQAAVGPRITARNFGKDRRYPITSGFGRKNW
Sequences:
>Translated_540_residues MSRSLSIALAQLNLLVGDIEGNTERMLQTVQEQQKAGADLVMFTELALSGYPPEDLLYRNDFYQRCDAQLQRLQQASAEV AILVGHPWREGDKLYNALSLFSEGQLLARYFKQQLPNYGVFDEKRYFQAGSETCVVELKGYQLGLLICEDLWFPGPVDAA KAAGAEMILSINASPYNREKPYIRKTLMAGHCQRTHLPLVYLNQIGGQDELIFDGCSKVFDAAGNMTHRLTAFAEQVTLL ELNELEVVPMTAPAAELPQLAQVYEALVLAVRDYVTKNGFKGAVLGLSGGIDSALTLAIAVDALGKDKVQALMMPFRYTA DISIADAKEEAEILGIEFDIVSIEPMFDAFMGQLTPMFAGTERDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMA VGYATLYGDMAGGFDVLKDVPKTLVFKLSEYRNTVSYVIPQRVIDRPPSAELAPDQLDQDSLPPYDILDAILEGYVERDK SVADLVAEGFDEAIVRKVIRLVDINEYKRRQAAVGPRITARNFGKDRRYPITSGFGRKNW >Mature_539_residues SRSLSIALAQLNLLVGDIEGNTERMLQTVQEQQKAGADLVMFTELALSGYPPEDLLYRNDFYQRCDAQLQRLQQASAEVA ILVGHPWREGDKLYNALSLFSEGQLLARYFKQQLPNYGVFDEKRYFQAGSETCVVELKGYQLGLLICEDLWFPGPVDAAK AAGAEMILSINASPYNREKPYIRKTLMAGHCQRTHLPLVYLNQIGGQDELIFDGCSKVFDAAGNMTHRLTAFAEQVTLLE LNELEVVPMTAPAAELPQLAQVYEALVLAVRDYVTKNGFKGAVLGLSGGIDSALTLAIAVDALGKDKVQALMMPFRYTAD ISIADAKEEAEILGIEFDIVSIEPMFDAFMGQLTPMFAGTERDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMAV GYATLYGDMAGGFDVLKDVPKTLVFKLSEYRNTVSYVIPQRVIDRPPSAELAPDQLDQDSLPPYDILDAILEGYVERDKS VADLVAEGFDEAIVRKVIRLVDINEYKRRQAAVGPRITARNFGKDRRYPITSGFGRKNW
Specific function: Can use both glutamine or ammonia as a nitrogen source [H]
COG id: COG0171
COG function: function code H; NAD synthase
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 CN hydrolase domain [H]
Homologues:
Organism=Saccharomyces cerevisiae, GI6321865, Length=299, Percent_Identity=26.7558528428094, Blast_Score=68, Evalue=4e-12,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014445 - InterPro: IPR022310 - InterPro: IPR003694 - InterPro: IPR000132 - InterPro: IPR003010 - InterPro: IPR014729 [H]
Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]
EC number: =6.3.5.1 [H]
Molecular weight: Translated: 59946; Mature: 59815
Theoretical pI: Translated: 4.71; Mature: 4.71
Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 2.8 %Met (Translated Protein) 3.9 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 2.6 %Met (Mature Protein) 3.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSRSLSIALAQLNLLVGDIEGNTERMLQTVQEQQKAGADLVMFTELALSGYPPEDLLYRN CCCCHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCHHHHHHH DFYQRCDAQLQRLQQASAEVAILVGHPWREGDKLYNALSLFSEGQLLARYFKQQLPNYGV HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC FDEKRYFQAGSETCVVELKGYQLGLLICEDLWFPGPVDAAKAAGAEMILSINASPYNREK CCCHHHHHCCCCEEEEEECCCEEEEEEEHHCCCCCCCCHHHHCCCEEEEEECCCCCCCCC PYIRKTLMAGHCQRTHLPLVYLNQIGGQDELIFDGCSKVFDAAGNMTHRLTAFAEQVTLL CHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHEEE ELNELEVVPMTAPAAELPQLAQVYEALVLAVRDYVTKNGFKGAVLGLSGGIDSALTLAIA EECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHH VDALGKDKVQALMMPFRYTADISIADAKEEAEILGIEFDIVSIEPMFDAFMGQLTPMFAG HHHCCHHHHHHHHHCCEEECCEEEECCCCCCEEEEEEEEEEEECHHHHHHHHHHHHHHCC TERDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMAVGYATLYGDMAGGFDVLKDV CCCCCCHHHHHHHHCEEEEEEECCCCCEEEEEECCCCCCEEEHHHHHHCCCCCHHHHHHH PKTLVFKLSEYRNTVSYVIPQRVIDRPPSAELAPDQLDQDSLPPYDILDAILEGYVERDK HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCH SVADLVAEGFDEAIVRKVIRLVDINEYKRRQAAVGPRITARNFGKDRRYPITSGFGRKNW HHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEHHCCCCCCCCCCCCCCCCCCC >Mature Secondary Structure SRSLSIALAQLNLLVGDIEGNTERMLQTVQEQQKAGADLVMFTELALSGYPPEDLLYRN CCCHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCHHHHHHH DFYQRCDAQLQRLQQASAEVAILVGHPWREGDKLYNALSLFSEGQLLARYFKQQLPNYGV HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC FDEKRYFQAGSETCVVELKGYQLGLLICEDLWFPGPVDAAKAAGAEMILSINASPYNREK CCCHHHHHCCCCEEEEEECCCEEEEEEEHHCCCCCCCCHHHHCCCEEEEEECCCCCCCCC PYIRKTLMAGHCQRTHLPLVYLNQIGGQDELIFDGCSKVFDAAGNMTHRLTAFAEQVTLL CHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHEEE ELNELEVVPMTAPAAELPQLAQVYEALVLAVRDYVTKNGFKGAVLGLSGGIDSALTLAIA EECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHH VDALGKDKVQALMMPFRYTADISIADAKEEAEILGIEFDIVSIEPMFDAFMGQLTPMFAG HHHCCHHHHHHHHHCCEEECCEEEECCCCCCEEEEEEEEEEEECHHHHHHHHHHHHHHCC TERDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMAVGYATLYGDMAGGFDVLKDV CCCCCCHHHHHHHHCEEEEEEECCCCCEEEEEECCCCCCEEEHHHHHHCCCCCHHHHHHH PKTLVFKLSEYRNTVSYVIPQRVIDRPPSAELAPDQLDQDSLPPYDILDAILEGYVERDK HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCH SVADLVAEGFDEAIVRKVIRLVDINEYKRRQAAVGPRITARNFGKDRRYPITSGFGRKNW HHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEHHCCCCCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10910347 [H]