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The map label for this gene is yfhA [H]

Identifier: 157371886

GI number: 157371886

Start: 4039187

End: 4040524

Strand: Reverse

Name: yfhA [H]

Synonym: Spro_3651

Alternate gene names: 157371886

Gene position: 4040524-4039187 (Counterclockwise)

Preceding gene: 157371887

Following gene: 157371885

Centisome position: 74.15

GC content: 58.45

Gene sequence:

>1338_bases
ATGACCGCACGCAAACCGGCCAATTTGTTATTGGTTGACGACGATCCCAGCCTGCTTAAGCTGCTGGGGATGCGCCTGAC
CAGCGAAGGATTTCATGTCACCACCGCCGAAAGCGGCCAGGAAGCGCTGCGCCTGTTAGGGCGTGAGAAGATTGATCTGG
TGATCAGCGATCTGCGGATGGATGAAATGGACGGCATGGCACTGTTTGCCGAAATCCAAAAGCATCAGCCCGGCATGCCG
GTGATCATTCTTACCGCCCATGGTTCAATCCCGGACGCGGTGGCGGCTACCCAGCAAGGGGTGTTCAGCTTCCTGACCAA
ACCGGTGGATCGTGATGCGCTGTATAAAGCCATCGACGATGCCCTATCGCTGTCACTGCCGGCCGGTGATGACAGTTGGC
GTGAAGACATCGTCACCCGCAGCCCGATTATGCTGCGCCTGCTGGAGCAGGCAAAAATGGTGGCGCAGTCGGACGTCAGC
GTATTGATTAACGGCCACAGCGGCACCGGTAAAGAGGTGCTGGCACAGGCGATCCACGGTGCCAGTCCTCGCGGTAAAAA
GGCGTTTATCGCCATTAACTGCGGGGCGCTGCCGGAGCAACTGCTGGAGTCCGAGCTGTTTGGCCATGCCAAAGGGGCCT
TTACCGGCGCGGTCAGCAGCCGCGAAGGGCTGTTCCAGGCGGCAGCCGGCGGCACGCTGTTCCTTGACGAGATCGGCGAT
ATGCCGCTTTCGCTGCAGGTTAAGCTGTTGCGCGTATTGCAGGAGCGCAAGGTGCGCCCGCTGGGCAGCAACCGCGATCT
GGACATTGACGTTCGCATCATTTCCGCCACTCACCGCGATCTGCAAAAGGCGATGGCGAAAAACGAATTCCGCGAGGATT
TGTACTACCGGCTGAACGTGGTGAACCTGAAGATCCCGGCGTTGAATGAGCGTGCCGAGGATATCCCGCTGCTGGCCAAT
CATCTGCTGCGCGAATCGGCCAAGCGCCATAAACCCTTCGTTCGCAGCTTCTCAACGGACGCTATGAAACGCCTGATGAC
CGCCACCTGGCCGGGCAACGTGCGCCAACTGGTGAACGTCATCGAGCAGTGTGTGGCCCTGACTTCTGCGCCGGTGATCA
GCGAAGCGCTGGTTGAGCAGGCGTTGGAAGGGGAAAATACCGCCTTGCCGACCTTTGTTGAGGCCCGCAATCAGTTTGAG
TTGCACTATCTGCGCAAGCTGTTGCAGATCACCAAGGGTAACGTGACCCAGGCCGCGCGCATGGCAGGCCGCAACCGTAC
CGAATTTTATAAGTTGCTGTCGCGTCATGAATTGGACGCCAACGATTTTAAAGAATGA

Upstream 100 bases:

>100_bases
CAGCAGAATCGGATGCTGAACCTGAGAAGGGTACCGCTTTGCCGGTGGAGCCGGAAGATACCTACGCGCCGCCCCCCGCT
AACAAGGAGTCTCACGCGCA

Downstream 100 bases:

>100_bases
TGTTTTCCGCCGCAGCCTGCGCACGCAGGTTCTCAACAAATGCGGCGGAATGATTTACACTCCGTTCTCTTGTTTGTGGC
GTGGCCCAACACAGAGCACC

Product: two component sigma54 specific Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MTARKPANLLLVDDDPSLLKLLGMRLTSEGFHVTTAESGQEALRLLGREKIDLVISDLRMDEMDGMALFAEIQKHQPGMP
VIILTAHGSIPDAVAATQQGVFSFLTKPVDRDALYKAIDDALSLSLPAGDDSWREDIVTRSPIMLRLLEQAKMVAQSDVS
VLINGHSGTGKEVLAQAIHGASPRGKKAFIAINCGALPEQLLESELFGHAKGAFTGAVSSREGLFQAAAGGTLFLDEIGD
MPLSLQVKLLRVLQERKVRPLGSNRDLDIDVRIISATHRDLQKAMAKNEFREDLYYRLNVVNLKIPALNERAEDIPLLAN
HLLRESAKRHKPFVRSFSTDAMKRLMTATWPGNVRQLVNVIEQCVALTSAPVISEALVEQALEGENTALPTFVEARNQFE
LHYLRKLLQITKGNVTQAARMAGRNRTEFYKLLSRHELDANDFKE

Sequences:

>Translated_445_residues
MTARKPANLLLVDDDPSLLKLLGMRLTSEGFHVTTAESGQEALRLLGREKIDLVISDLRMDEMDGMALFAEIQKHQPGMP
VIILTAHGSIPDAVAATQQGVFSFLTKPVDRDALYKAIDDALSLSLPAGDDSWREDIVTRSPIMLRLLEQAKMVAQSDVS
VLINGHSGTGKEVLAQAIHGASPRGKKAFIAINCGALPEQLLESELFGHAKGAFTGAVSSREGLFQAAAGGTLFLDEIGD
MPLSLQVKLLRVLQERKVRPLGSNRDLDIDVRIISATHRDLQKAMAKNEFREDLYYRLNVVNLKIPALNERAEDIPLLAN
HLLRESAKRHKPFVRSFSTDAMKRLMTATWPGNVRQLVNVIEQCVALTSAPVISEALVEQALEGENTALPTFVEARNQFE
LHYLRKLLQITKGNVTQAARMAGRNRTEFYKLLSRHELDANDFKE
>Mature_444_residues
TARKPANLLLVDDDPSLLKLLGMRLTSEGFHVTTAESGQEALRLLGREKIDLVISDLRMDEMDGMALFAEIQKHQPGMPV
IILTAHGSIPDAVAATQQGVFSFLTKPVDRDALYKAIDDALSLSLPAGDDSWREDIVTRSPIMLRLLEQAKMVAQSDVSV
LINGHSGTGKEVLAQAIHGASPRGKKAFIAINCGALPEQLLESELFGHAKGAFTGAVSSREGLFQAAAGGTLFLDEIGDM
PLSLQVKLLRVLQERKVRPLGSNRDLDIDVRIISATHRDLQKAMAKNEFREDLYYRLNVVNLKIPALNERAEDIPLLANH
LLRESAKRHKPFVRSFSTDAMKRLMTATWPGNVRQLVNVIEQCVALTSAPVISEALVEQALEGENTALPTFVEARNQFEL
HYLRKLLQITKGNVTQAARMAGRNRTEFYKLLSRHELDANDFKE

Specific function: Probable member of a two-component regulatory system yfhA/yfhK [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=443, Percent_Identity=87.3589164785553, Blast_Score=778, Evalue=0.0,
Organism=Escherichia coli, GI1790437, Length=440, Percent_Identity=44.7727272727273, Blast_Score=353, Evalue=1e-98,
Organism=Escherichia coli, GI1788550, Length=454, Percent_Identity=41.8502202643172, Blast_Score=335, Evalue=4e-93,
Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=39.5299145299145, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI87082117, Length=233, Percent_Identity=54.9356223175966, Blast_Score=264, Evalue=7e-72,
Organism=Escherichia coli, GI1789087, Length=329, Percent_Identity=44.9848024316109, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1789233, Length=253, Percent_Identity=48.2213438735178, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI87082152, Length=316, Percent_Identity=39.5569620253165, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI1786524, Length=319, Percent_Identity=40.4388714733542, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI87081872, Length=322, Percent_Identity=36.3354037267081, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1787583, Length=238, Percent_Identity=41.5966386554622, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1789828, Length=275, Percent_Identity=36.7272727272727, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI87081858, Length=310, Percent_Identity=31.6129032258064, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI145693157, Length=110, Percent_Identity=35.4545454545455, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789809, Length=103, Percent_Identity=32.0388349514563, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49197; Mature: 49066

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARKPANLLLVDDDPSLLKLLGMRLTSEGFHVTTAESGQEALRLLGREKIDLVISDLRM
CCCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHH
DEMDGMALFAEIQKHQPGMPVIILTAHGSIPDAVAATQQGVFSFLTKPVDRDALYKAIDD
HHCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALSLSLPAGDDSWREDIVTRSPIMLRLLEQAKMVAQSDVSVLINGHSGTGKEVLAQAIHG
HHEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHC
ASPRGKKAFIAINCGALPEQLLESELFGHAKGAFTGAVSSREGLFQAAAGGTLFLDEIGD
CCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCHHCCCCCCCCHHHHCCCCEEEEHHHCC
MPLSLQVKLLRVLQERKVRPLGSNRDLDIDVRIISATHRDLQKAMAKNEFREDLYYRLNV
CCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHEEEEE
VNLKIPALNERAEDIPLLANHLLRESAKRHKPFVRSFSTDAMKRLMTATWPGNVRQLVNV
EEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHCCCCCCHHHHHHH
IEQCVALTSAPVISEALVEQALEGENTALPTFVEARNQFELHYLRKLLQITKGNVTQAAR
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHH
MAGRNRTEFYKLLSRHELDANDFKE
HHCCCHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
TARKPANLLLVDDDPSLLKLLGMRLTSEGFHVTTAESGQEALRLLGREKIDLVISDLRM
CCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHH
DEMDGMALFAEIQKHQPGMPVIILTAHGSIPDAVAATQQGVFSFLTKPVDRDALYKAIDD
HHCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALSLSLPAGDDSWREDIVTRSPIMLRLLEQAKMVAQSDVSVLINGHSGTGKEVLAQAIHG
HHEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHC
ASPRGKKAFIAINCGALPEQLLESELFGHAKGAFTGAVSSREGLFQAAAGGTLFLDEIGD
CCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCHHCCCCCCCCHHHHCCCCEEEEHHHCC
MPLSLQVKLLRVLQERKVRPLGSNRDLDIDVRIISATHRDLQKAMAKNEFREDLYYRLNV
CCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHEEEEE
VNLKIPALNERAEDIPLLANHLLRESAKRHKPFVRSFSTDAMKRLMTATWPGNVRQLVNV
EEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHCCCCCCHHHHHHH
IEQCVALTSAPVISEALVEQALEGENTALPTFVEARNQFELHYLRKLLQITKGNVTQAAR
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHH
MAGRNRTEFYKLLSRHELDANDFKE
HHCCCHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]