Click here to switch to the map view.

The map label for this gene is trmJ [H]

Identifier: 157371864

GI number: 157371864

Start: 4013716

End: 4014450

Strand: Reverse

Name: trmJ [H]

Synonym: Spro_3629

Alternate gene names: 157371864

Gene position: 4014450-4013716 (Counterclockwise)

Preceding gene: 157371866

Following gene: 157371863

Centisome position: 73.68

GC content: 56.05

Gene sequence:

>735_bases
ATGCTGCATAACATCCGCATTGTTCTGGTAGAAACCTCTCATACCGGTAACATGGGCTCGACCGCCAGAGCCATGAAAAC
CATGGGATTAACCAACCTTTATTTGGTTAATCCGCTGATAAAACCTGATTCTCAGGCGATCGCCCTGGCCGCTGGTGCCA
GCGACGTGATCGGCAACGCCACTATTGTTGACACTCTGGATGACGCCATTGCCGGCTGCAGTCTGGTCGTGGGCACCAGT
GCGCGTTCGCGCACCTTGCCGTGGCCGATGCTGGAACCGCGCGAATGCGGCATCCGCGCCGTGCACGAAGGTGAACATGC
GCCGGTAGCGCTGGTGTTCGGCCGTGAACGTGTCGGCCTGACCAATGATGAGCTGCAAAAGTGCCATTATCATGTCGCCA
TCCCTGCCAACCCGGATTACAGCTCGTTGAATCTGGCGATGGCGGTGCAAATTCTGGCCTATGAAGTGCGTGTGGCTTAC
CTCGATCGTCAACAGGCAGGCGCGCCACAACTGGAAGAAACGCCGTATCCGTTGGTGGATGACCTGGAGCGTTTCTATCA
GCATCTGGAACAAACGCTGCAGCATAGTGGCTTTATCCGCCCCTCTCATCCGGGGCAGGTCATGAGCCGTTTGCGTCGTT
TATTCACCCGTGCGCGGCCGGAAGCTCAAGAGCTGAATATCCTGCGCGGTATGCTGACGTCGATCGAGAAACAGGACAAA
CATCAAGGTAATTAA

Upstream 100 bases:

>100_bases
TGTTAAAGAACAGGAAACGGCGCGAAGTATAGCAGGTGTTCGGCAAAATGCCTACGGCTGTGTTAAGCTGCTGTGATTTT
CCGCTACGCTCAGAGTTTGT

Downstream 100 bases:

>100_bases
TTTGATGTGAAATGACGGCTTGCGGTAATAGTATTAGTTATCTAACGGTTATAATGTGTATTTTATATGCATATTATCTG
GCTTAGTCCAAAACGTGCTA

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MLHNIRIVLVETSHTGNMGSTARAMKTMGLTNLYLVNPLIKPDSQAIALAAGASDVIGNATIVDTLDDAIAGCSLVVGTS
ARSRTLPWPMLEPRECGIRAVHEGEHAPVALVFGRERVGLTNDELQKCHYHVAIPANPDYSSLNLAMAVQILAYEVRVAY
LDRQQAGAPQLEETPYPLVDDLERFYQHLEQTLQHSGFIRPSHPGQVMSRLRRLFTRARPEAQELNILRGMLTSIEKQDK
HQGN

Sequences:

>Translated_244_residues
MLHNIRIVLVETSHTGNMGSTARAMKTMGLTNLYLVNPLIKPDSQAIALAAGASDVIGNATIVDTLDDAIAGCSLVVGTS
ARSRTLPWPMLEPRECGIRAVHEGEHAPVALVFGRERVGLTNDELQKCHYHVAIPANPDYSSLNLAMAVQILAYEVRVAY
LDRQQAGAPQLEETPYPLVDDLERFYQHLEQTLQHSGFIRPSHPGQVMSRLRRLFTRARPEAQELNILRGMLTSIEKQDK
HQGN
>Mature_244_residues
MLHNIRIVLVETSHTGNMGSTARAMKTMGLTNLYLVNPLIKPDSQAIALAAGASDVIGNATIVDTLDDAIAGCSLVVGTS
ARSRTLPWPMLEPRECGIRAVHEGEHAPVALVFGRERVGLTNDELQKCHYHVAIPANPDYSSLNLAMAVQILAYEVRVAY
LDRQQAGAPQLEETPYPLVDDLERFYQHLEQTLQHSGFIRPSHPGQVMSRLRRLFTRARPEAQELNILRGMLTSIEKQDK
HQGN

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=241, Percent_Identity=81.3278008298755, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI1790865, Length=161, Percent_Identity=36.0248447204969, Blast_Score=87, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26978; Mature: 26978

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHNIRIVLVETSHTGNMGSTARAMKTMGLTNLYLVNPLIKPDSQAIALAAGASDVIGNA
CCCCEEEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCHHHHCCC
TIVDTLDDAIAGCSLVVGTSARSRTLPWPMLEPRECGIRAVHEGEHAPVALVFGRERVGL
EEHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHCCCEEEECCCCCCEEEEECCCCCCC
TNDELQKCHYHVAIPANPDYSSLNLAMAVQILAYEVRVAYLDRQQAGAPQLEETPYPLVD
CHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
DLERFYQHLEQTLQHSGFIRPSHPGQVMSRLRRLFTRARPEAQELNILRGMLTSIEKQDK
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
HQGN
CCCC
>Mature Secondary Structure
MLHNIRIVLVETSHTGNMGSTARAMKTMGLTNLYLVNPLIKPDSQAIALAAGASDVIGNA
CCCCEEEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCHHHHCCC
TIVDTLDDAIAGCSLVVGTSARSRTLPWPMLEPRECGIRAVHEGEHAPVALVFGRERVGL
EEHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHCCCEEEECCCCCCEEEEECCCCCCC
TNDELQKCHYHVAIPANPDYSSLNLAMAVQILAYEVRVAYLDRQQAGAPQLEETPYPLVD
CHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
DLERFYQHLEQTLQHSGFIRPSHPGQVMSRLRRLFTRARPEAQELNILRGMLTSIEKQDK
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
HQGN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA