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The map label for this gene is apbE [H]

Identifier: 157371598

GI number: 157371598

Start: 3719469

End: 3720500

Strand: Direct

Name: apbE [H]

Synonym: Spro_3361

Alternate gene names: 157371598

Gene position: 3719469-3720500 (Clockwise)

Preceding gene: 157371596

Following gene: 157371606

Centisome position: 68.26

GC content: 56.88

Gene sequence:

>1032_bases
ATGGCTAACGTTTTCGCCAAAGGGCTGTTACTGAGCATGACGCTCGGCTTGCTGACCGCCTGTAATGACCAGAATACCCG
CCCGCAAATGGACATCGACGGCAAAACCATGGGCACCTTCTACAGCGTCAAGGTCAGCGGTGATGTGGCAGTCGATAAAC
AACAGTTACAGCAGCAGATCGACGCCGTGCTGGAACGCGCCAACGACGATATCTCCACCTATCGCAATGATTCGGTGCTG
TCGCGCTTTAATCAGAGCAGCAGCACAGAGCCACAGCCGATCCCGCGCGGCATGGCGGACATTATCCTGATGGCCCAACG
CATCGGCCGCGACACTCAGGGCGCAATGGATATTACCGTCGGCCCGCTGGTCAATTTGTGGGGCTTCGGCCCGGATAAGC
GAGTGGTCAAGGTACCGAGCCAACAACAAATAGAAACGGCGCAAAAGAACGTCGGCTTGCGGCACCTCAAACTGATCGGT
GACAACCACGGCGAATGGCTGCAAAAAGACTTGCCTGAAATGTATGTTGATCTCTCAACGCTGGGTGAAGGCTATGGCGT
TGACCAGTTGGTGCAATTGATGGCGCGCAACGGCATCACCAACTATCTGGTCTCGGTTGGCGGTGCGGTTTCCTCCCGCG
GCGTTAACGGCCAGGGCAAAGCCTGGCGGGTGGCGATCCAAAAGCCTACCGACCGCGAAAATGCAGTACAGGCTTTGGTC
GATTTACAAGGTTATGGCATCAGTACCGCCGGTAGTTACCGCAATTATTTCGAACAGGATGGCCAGCGCTATTCGCACGT
GATCGATCCCACCACCGGCCGGCCGATAACCCACCGTCTGGTGTCCGCCACGGTGATCGCCCCGACCGCGCTGGAAGCCG
ACGGTTGGGATACCGGACTGATGGTGCTGGGGACGGAAAAGGCCCTGAAACTGGCGCAGGAAAAAGGACTGGCGGTCTAT
CTGATCAGCAAAACCGACGAAGGGTTCAGCGCCGTGATGACGCCCCAGTTTAAAGCCTTCCTGGTAAAGTAA

Upstream 100 bases:

>100_bases
ACGGCAACGCCAGTAAAATGCTCTTTTACCTTTATCTGCTTTTCATTCGCCGCACTTTGCTTTATTTGTAGCGCCTTTCC
CAGATTTGAAGGATTTCTTT

Downstream 100 bases:

>100_bases
TTTCTGGTTCGGTATTAAGGGGCGCCGTTCAATACCGCGCCCCTTGAACGACTGACAAAGCCCGTTATTAGGGAGAGCGT
GCCGAAGGGGTCGTAACGGC

Product: thiamine biosynthesis lipoprotein ApbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MANVFAKGLLLSMTLGLLTACNDQNTRPQMDIDGKTMGTFYSVKVSGDVAVDKQQLQQQIDAVLERANDDISTYRNDSVL
SRFNQSSSTEPQPIPRGMADIILMAQRIGRDTQGAMDITVGPLVNLWGFGPDKRVVKVPSQQQIETAQKNVGLRHLKLIG
DNHGEWLQKDLPEMYVDLSTLGEGYGVDQLVQLMARNGITNYLVSVGGAVSSRGVNGQGKAWRVAIQKPTDRENAVQALV
DLQGYGISTAGSYRNYFEQDGQRYSHVIDPTTGRPITHRLVSATVIAPTALEADGWDTGLMVLGTEKALKLAQEKGLAVY
LISKTDEGFSAVMTPQFKAFLVK

Sequences:

>Translated_343_residues
MANVFAKGLLLSMTLGLLTACNDQNTRPQMDIDGKTMGTFYSVKVSGDVAVDKQQLQQQIDAVLERANDDISTYRNDSVL
SRFNQSSSTEPQPIPRGMADIILMAQRIGRDTQGAMDITVGPLVNLWGFGPDKRVVKVPSQQQIETAQKNVGLRHLKLIG
DNHGEWLQKDLPEMYVDLSTLGEGYGVDQLVQLMARNGITNYLVSVGGAVSSRGVNGQGKAWRVAIQKPTDRENAVQALV
DLQGYGISTAGSYRNYFEQDGQRYSHVIDPTTGRPITHRLVSATVIAPTALEADGWDTGLMVLGTEKALKLAQEKGLAVY
LISKTDEGFSAVMTPQFKAFLVK
>Mature_342_residues
ANVFAKGLLLSMTLGLLTACNDQNTRPQMDIDGKTMGTFYSVKVSGDVAVDKQQLQQQIDAVLERANDDISTYRNDSVLS
RFNQSSSTEPQPIPRGMADIILMAQRIGRDTQGAMDITVGPLVNLWGFGPDKRVVKVPSQQQIETAQKNVGLRHLKLIGD
NHGEWLQKDLPEMYVDLSTLGEGYGVDQLVQLMARNGITNYLVSVGGAVSSRGVNGQGKAWRVAIQKPTDRENAVQALVD
LQGYGISTAGSYRNYFEQDGQRYSHVIDPTTGRPITHRLVSATVIAPTALEADGWDTGLMVLGTEKALKLAQEKGLAVYL
ISKTDEGFSAVMTPQFKAFLVK

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=345, Percent_Identity=56.231884057971, Blast_Score=399, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 37434; Mature: 37303

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVFAKGLLLSMTLGLLTACNDQNTRPQMDIDGKTMGTFYSVKVSGDVAVDKQQLQQQI
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECEEEEEEECCCCCCCHHHHHHHH
DAVLERANDDISTYRNDSVLSRFNQSSSTEPQPIPRGMADIILMAQRIGRDTQGAMDITV
HHHHHHCCCHHHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEH
GPLVNLWGFGPDKRVVKVPSQQQIETAQKNVGLRHLKLIGDNHGEWLQKDLPEMYVDLST
HHHHHHHCCCCCCCEEECCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
LGEGYGVDQLVQLMARNGITNYLVSVGGAVSSRGVNGQGKAWRVAIQKPTDRENAVQALV
CCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHCCCCCCCCCEEEEEEECCCCHHHHHHHHH
DLQGYGISTAGSYRNYFEQDGQRYSHVIDPTTGRPITHRLVSATVIAPTALEADGWDTGL
HHCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHEECCCEECCCCCCCCE
MVLGTEKALKLAQEKGLAVYLISKTDEGFSAVMTPQFKAFLVK
EEEECHHHHHHHHHCCCEEEEEECCCCCCEEEECCCCEEEECC
>Mature Secondary Structure 
ANVFAKGLLLSMTLGLLTACNDQNTRPQMDIDGKTMGTFYSVKVSGDVAVDKQQLQQQI
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECEEEEEEECCCCCCCHHHHHHHH
DAVLERANDDISTYRNDSVLSRFNQSSSTEPQPIPRGMADIILMAQRIGRDTQGAMDITV
HHHHHHCCCHHHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEH
GPLVNLWGFGPDKRVVKVPSQQQIETAQKNVGLRHLKLIGDNHGEWLQKDLPEMYVDLST
HHHHHHHCCCCCCCEEECCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
LGEGYGVDQLVQLMARNGITNYLVSVGGAVSSRGVNGQGKAWRVAIQKPTDRENAVQALV
CCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHCCCCCCCCCEEEEEEECCCCHHHHHHHHH
DLQGYGISTAGSYRNYFEQDGQRYSHVIDPTTGRPITHRLVSATVIAPTALEADGWDTGL
HHCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHEECCCEECCCCCCCCE
MVLGTEKALKLAQEKGLAVYLISKTDEGFSAVMTPQFKAFLVK
EEEECHHHHHHHHHCCCEEEEEECCCCCCEEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9473043; 11677609; 1904855 [H]