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The map label for this gene is mnmC

Identifier: 157371606

GI number: 157371606

Start: 3729378

End: 3731399

Strand: Direct

Name: mnmC

Synonym: Spro_3369

Alternate gene names: 157371606

Gene position: 3729378-3731399 (Clockwise)

Preceding gene: 157371598

Following gene: 157371613

Centisome position: 68.44

GC content: 60.88

Gene sequence:

>2022_bases
GTGAGCCACGCCCCGATCCAAACCGCAGCGTTATCCTGGAATGAACAGGGTACACCTGTTTCGAAACAGTTTGATGACGT
CTACTTTTCTAATCAGGATGGGCTGGAAGAAACCCGCTACGTGTTTCTCAAGGGCAACCGGCTGCCGTCACGTTTTGCCG
AACACCCTCGCCCGCTGTTTATCGTGGCGGAAACCGGCTTCGGCACCGGACTTAATTTTTTAACCCTGTGGCAGGCCTTT
GCCGATTTTCATGCCACAACGCCGGATGCCACGCTGCAGCGGCTGCACTTTATCAGCTTTGAAAAATTCCCGCTGCTGCA
GGCCGATCTGGCGGCGGCACATGCCCGTTGGCCAGAGCTGGCTCCCTATGCCGACGAGTTACGCGCCCAATGGCCGTTGC
CGCTGCCGGGCTGCCACCGCCTGTTGCTGGCGGAAGGCCGCATTACGCTGGATCTGTGGTTCGGTGACGTGAACGAATTG
CTACCACACTTCGACGCCAGCATGCACCGCCAAATCGATGCCTGGTTCCTCGACGGCTTCGCCCCGTCGAAAAATCCGGA
TATGTGGACCGAGCAGCTGTTCGCCGCCATGGCGCGCTTTGCCCGGCCGGGGGGCAGCCTGGCCACCTTCACCGCAGCAG
GTTTTGTTCGGCGCGGGCTGCAGCAGGCCGGATTTGAAGTCATAAAAAGCAAAGGTTTCGGCCAGAAACGTGAAATGCTG
ATTGGTGAGCTGCCTGCCGATGCACCAGCGGTGGCCCACCCCACGCCCTGGTTACTGCGCCCGGCAGCCGACAATACCGA
CGATATTGCTATCATTGGCGGCGGCGTTGCCAGCGCCTTGACTGCCCTCGCCTTGCTGCGCCGTGGTGCGAAGGTCACGC
TGTACTGCGCCGATCCTCAGGCGGCCGAAGGGGCATCGGGCAACCGTCAGGGAGCGCTTTATCCGTTGCTGAACGGCCGC
GGCGATGCCCTGGAAAACTTCTTCAGCGCCGCTTTCCTTTTTGCCCGCCGCCAGTACGATGCGCTGCTAAAACAGGGCGT
GACGTTTGACCACCAGTGGTGTGGCGTCAGCCAATTGGCCTATGATGAAAAAAGTGCCGGGAAGATTGCCAACATGCTGG
CGGTTGAATGGCCACAACAACTGGCCGTGGCGGCCGATCGCACCACGCTCAGCGCCCTGTGCGGTATCGACAGTGGTTTT
GGCGGCATTAACTACCCGCAGGGTGGCTGGCTGTGCCCGGCCGAGCTGACGCGCAATGCGATAGCCCTGGCGCAACATCA
GGGGTTAAGCTGTCACTATGAGTCTGACGTCAAAGCCCTGACCGCCACCGACAGCGGCTGGCAGCTCAGTTTCAACCAGG
AGGAAACCCGACAGCATGCGACGGTGATCCTGGCCAACGGCCATCGGCTTGGCGAACTGGCGCCCAGCGAAGCGCTGCCG
GTTTATGCGGTGCGCGGTCAAGTCTCCCATATTCCCACCACCCCAGCGCTGAGCGAATTGAAACAGGTGCTGTGTTACGA
CGGCTACCTGACGCCGGTCAATGCCAACAACCAACAGCACTGCATCGGTGCCAGCTACCAGCGCGGCGAAACCGCCACCG
AATACCGCGAGGAAGAACAGCAGGACAACCGTGCTCGCCTGCTGCGCTGCCTGCCGGAACAGAGCTGGCCAGCACAGGTT
GATGTCAGCGGTCAGCATGCCCGCTGCGGCGTGCGCAGCGCCACCCGCGATCATCTGCCGATGATCGGCGCAGTACCGGA
CTATCAGGCTACGCTGACGCAGTATCAGCATCTGCAGCGCCAGCATCAACGCGGCGAGGCTATCGCCAATGCACCGAGCT
ATCCCAATCTGTTTGTGATTGGCGCGTTGGGTTCGCGTGGGCTGTGCTCAGCACCGCTGGCGGCGGAAATTTTGGCGGCG
CAGCTGTTCGGCGAACCCTTGCCGGGCGACGCAGACATGCTGGCAGCATTGAATCCTAATCGGATGTGGGTAAGGAAACT
ACTGAAGGGACGTGCGGTTTAA

Upstream 100 bases:

>100_bases
AAGTCCGATCAGCGTTTTTCAGCTTTATTCTTATCCAGTTTGCGCCATAGCGCCAGCGCCGTTAGAATCCCGTCACCCCT
TGAGTTACGAGCAACCAACC

Downstream 100 bases:

>100_bases
TGACGGTGTTACGAGGCGCTGTTTTCAGCGCCTCGTAAAACGGATCAGGCGGTCGGCAGTGCGGTTTGGTACAGATTTTC
CCACATGCCATACACCAACA

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MSHAPIQTAALSWNEQGTPVSKQFDDVYFSNQDGLEETRYVFLKGNRLPSRFAEHPRPLFIVAETGFGTGLNFLTLWQAF
ADFHATTPDATLQRLHFISFEKFPLLQADLAAAHARWPELAPYADELRAQWPLPLPGCHRLLLAEGRITLDLWFGDVNEL
LPHFDASMHRQIDAWFLDGFAPSKNPDMWTEQLFAAMARFARPGGSLATFTAAGFVRRGLQQAGFEVIKSKGFGQKREML
IGELPADAPAVAHPTPWLLRPAADNTDDIAIIGGGVASALTALALLRRGAKVTLYCADPQAAEGASGNRQGALYPLLNGR
GDALENFFSAAFLFARRQYDALLKQGVTFDHQWCGVSQLAYDEKSAGKIANMLAVEWPQQLAVAADRTTLSALCGIDSGF
GGINYPQGGWLCPAELTRNAIALAQHQGLSCHYESDVKALTATDSGWQLSFNQEETRQHATVILANGHRLGELAPSEALP
VYAVRGQVSHIPTTPALSELKQVLCYDGYLTPVNANNQQHCIGASYQRGETATEYREEEQQDNRARLLRCLPEQSWPAQV
DVSGQHARCGVRSATRDHLPMIGAVPDYQATLTQYQHLQRQHQRGEAIANAPSYPNLFVIGALGSRGLCSAPLAAEILAA
QLFGEPLPGDADMLAALNPNRMWVRKLLKGRAV

Sequences:

>Translated_673_residues
MSHAPIQTAALSWNEQGTPVSKQFDDVYFSNQDGLEETRYVFLKGNRLPSRFAEHPRPLFIVAETGFGTGLNFLTLWQAF
ADFHATTPDATLQRLHFISFEKFPLLQADLAAAHARWPELAPYADELRAQWPLPLPGCHRLLLAEGRITLDLWFGDVNEL
LPHFDASMHRQIDAWFLDGFAPSKNPDMWTEQLFAAMARFARPGGSLATFTAAGFVRRGLQQAGFEVIKSKGFGQKREML
IGELPADAPAVAHPTPWLLRPAADNTDDIAIIGGGVASALTALALLRRGAKVTLYCADPQAAEGASGNRQGALYPLLNGR
GDALENFFSAAFLFARRQYDALLKQGVTFDHQWCGVSQLAYDEKSAGKIANMLAVEWPQQLAVAADRTTLSALCGIDSGF
GGINYPQGGWLCPAELTRNAIALAQHQGLSCHYESDVKALTATDSGWQLSFNQEETRQHATVILANGHRLGELAPSEALP
VYAVRGQVSHIPTTPALSELKQVLCYDGYLTPVNANNQQHCIGASYQRGETATEYREEEQQDNRARLLRCLPEQSWPAQV
DVSGQHARCGVRSATRDHLPMIGAVPDYQATLTQYQHLQRQHQRGEAIANAPSYPNLFVIGALGSRGLCSAPLAAEILAA
QLFGEPLPGDADMLAALNPNRMWVRKLLKGRAV
>Mature_672_residues
SHAPIQTAALSWNEQGTPVSKQFDDVYFSNQDGLEETRYVFLKGNRLPSRFAEHPRPLFIVAETGFGTGLNFLTLWQAFA
DFHATTPDATLQRLHFISFEKFPLLQADLAAAHARWPELAPYADELRAQWPLPLPGCHRLLLAEGRITLDLWFGDVNELL
PHFDASMHRQIDAWFLDGFAPSKNPDMWTEQLFAAMARFARPGGSLATFTAAGFVRRGLQQAGFEVIKSKGFGQKREMLI
GELPADAPAVAHPTPWLLRPAADNTDDIAIIGGGVASALTALALLRRGAKVTLYCADPQAAEGASGNRQGALYPLLNGRG
DALENFFSAAFLFARRQYDALLKQGVTFDHQWCGVSQLAYDEKSAGKIANMLAVEWPQQLAVAADRTTLSALCGIDSGFG
GINYPQGGWLCPAELTRNAIALAQHQGLSCHYESDVKALTATDSGWQLSFNQEETRQHATVILANGHRLGELAPSEALPV
YAVRGQVSHIPTTPALSELKQVLCYDGYLTPVNANNQQHCIGASYQRGETATEYREEEQQDNRARLLRCLPEQSWPAQVD
VSGQHARCGVRSATRDHLPMIGAVPDYQATLTQYQHLQRQHQRGEAIANAPSYPNLFVIGALGSRGLCSAPLAAEILAAQ
LFGEPLPGDADMLAALNPNRMWVRKLLKGRAV

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=673, Percent_Identity=63.150074294205, Blast_Score=856, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_SERP5 (A8GH77)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479595.1
- ProteinModelPortal:   A8GH77
- SMR:   A8GH77
- STRING:   A8GH77
- GeneID:   5602982
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3369
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- ProtClustDB:   PRK01747
- BioCyc:   SPRO399741:SPRO_3369-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 73710; Mature: 73579

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHAPIQTAALSWNEQGTPVSKQFDDVYFSNQDGLEETRYVFLKGNRLPSRFAEHPRPLF
CCCCCCCCEEECCCCCCCCCHHHHHHHEECCCCCCCCEEEEEEECCCCCHHHHCCCCCEE
IVAETGFGTGLNFLTLWQAFADFHATTPDATLQRLHFISFEKFPLLQADLAAAHARWPEL
EEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
APYADELRAQWPLPLPGCHRLLLAEGRITLDLWFGDVNELLPHFDASMHRQIDAWFLDGF
CCCHHHHCCCCCCCCCCHHHHEEECCCEEEEEEECCHHHHHHHCCHHHHHHHHHHEECCC
APSKNPDMWTEQLFAAMARFARPGGSLATFTAAGFVRRGLQQAGFEVIKSKGFGQKREML
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IGELPADAPAVAHPTPWLLRPAADNTDDIAIIGGGVASALTALALLRRGAKVTLYCADPQ
HHCCCCCCCCCCCCCCCEEECCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCC
AAEGASGNRQGALYPLLNGRGDALENFFSAAFLFARRQYDALLKQGVTFDHQWCGVSQLA
CCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
YDEKSAGKIANMLAVEWPQQLAVAADRTTLSALCGIDSGFGGINYPQGGWLCPAELTRNA
CCCHHHHHHHHHHHHHCHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHH
IALAQHQGLSCHYESDVKALTATDSGWQLSFNQEETRQHATVILANGHRLGELAPSEALP
HHHHHCCCCCEEECCCCEEEEECCCCCEEECCHHHHCCEEEEEEECCCCCCCCCCCCCCC
VYAVRGQVSHIPTTPALSELKQVLCYDGYLTPVNANNQQHCIGASYQRGETATEYREEEQ
EEEECCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCEECCCCCCCCCHHHHHHHHH
QDNRARLLRCLPEQSWPAQVDVSGQHARCGVRSATRDHLPMIGAVPDYQATLTQYQHLQR
HHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHH
QHQRGEAIANAPSYPNLFVIGALGSRGLCSAPLAAEILAAQLFGEPLPGDADMLAALNPN
HHHHCCHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCC
RMWVRKLLKGRAV
HHHHHHHHHCCCC
>Mature Secondary Structure 
SHAPIQTAALSWNEQGTPVSKQFDDVYFSNQDGLEETRYVFLKGNRLPSRFAEHPRPLF
CCCCCCCEEECCCCCCCCCHHHHHHHEECCCCCCCCEEEEEEECCCCCHHHHCCCCCEE
IVAETGFGTGLNFLTLWQAFADFHATTPDATLQRLHFISFEKFPLLQADLAAAHARWPEL
EEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
APYADELRAQWPLPLPGCHRLLLAEGRITLDLWFGDVNELLPHFDASMHRQIDAWFLDGF
CCCHHHHCCCCCCCCCCHHHHEEECCCEEEEEEECCHHHHHHHCCHHHHHHHHHHEECCC
APSKNPDMWTEQLFAAMARFARPGGSLATFTAAGFVRRGLQQAGFEVIKSKGFGQKREML
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IGELPADAPAVAHPTPWLLRPAADNTDDIAIIGGGVASALTALALLRRGAKVTLYCADPQ
HHCCCCCCCCCCCCCCCEEECCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCC
AAEGASGNRQGALYPLLNGRGDALENFFSAAFLFARRQYDALLKQGVTFDHQWCGVSQLA
CCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
YDEKSAGKIANMLAVEWPQQLAVAADRTTLSALCGIDSGFGGINYPQGGWLCPAELTRNA
CCCHHHHHHHHHHHHHCHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHH
IALAQHQGLSCHYESDVKALTATDSGWQLSFNQEETRQHATVILANGHRLGELAPSEALP
HHHHHCCCCCEEECCCCEEEEECCCCCEEECCHHHHCCEEEEEEECCCCCCCCCCCCCCC
VYAVRGQVSHIPTTPALSELKQVLCYDGYLTPVNANNQQHCIGASYQRGETATEYREEEQ
EEEECCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCEECCCCCCCCCHHHHHHHHH
QDNRARLLRCLPEQSWPAQVDVSGQHARCGVRSATRDHLPMIGAVPDYQATLTQYQHLQR
HHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHH
QHQRGEAIANAPSYPNLFVIGALGSRGLCSAPLAAEILAAQLFGEPLPGDADMLAALNPN
HHHHCCHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCC
RMWVRKLLKGRAV
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA