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The map label for this gene is puuR [H]

Identifier: 157371454

GI number: 157371454

Start: 3544414

End: 3544971

Strand: Direct

Name: puuR [H]

Synonym: Spro_3217

Alternate gene names: 157371454

Gene position: 3544414-3544971 (Clockwise)

Preceding gene: 157371451

Following gene: 157371455

Centisome position: 65.05

GC content: 57.89

Gene sequence:

>558_bases
ATGAGCGAAGTCAGCTTGGCGCCGGGTAAGCGCCTGTCACAGATCCGTCTGCAATTGGGTTTGTCGCAGCGCCGGGTCGC
CGAACTGTCCGGGTTAACCCACAGCGCAATCAGCACCATCGAACAGGACAAGGTCAGTCCGGCCATCAGTACGCTGCAAA
AGTTGCTGAAGGTCTATGGACTGTCGCTGTCTGCATTCTTTGCCGAGCCAGAGGCCGCCAATGAACCCAAAGTGGTGATC
GAGGCCGAAGACCTGATCGAAATCGGCAGTCAGGGTGTCTCGATGAAGCTGGTACACAACGGCAACCCAACCCGCAATCT
GGCAATGATGCTGGAAACCTACCAGCCCGGAACCACCACCGGAGAGAAAATCAAGCACCAGGGCGAGGAGATCGGTACCC
TGCTGGAAGGCGAGATAATGCTGACCATCAACGGTCAGACTCACTGCCTGACCGCCGGTCAGAGCTATGCCATCAACACC
GGCATTCCCCACAGTTTCAGCAATACCTCGGCGCGCGTCTGTCGCATCGTCAGCGCCCATACCCCGACGACCTTCTGA

Upstream 100 bases:

>100_bases
TCGCAACTACCGATATCATGTTGTGTCGAAGCCACTCGAACAGGTACCTGACACCGATATCCATTTCAGCCTGTCGTCCC
TACCAGAAGGAAGTCCCTCT

Downstream 100 bases:

>100_bases
TAAACCGGACCAGGGGATGGCGCTATCAGCGATTCCCTTTCCGCGGCATCCCGTTCAGTTTTGCCATCGCCTCTGAACGG
GCGCCCTCATCGGCGAAAGA

Product: DNA-binding transcriptional repressor PuuR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MSEVSLAPGKRLSQIRLQLGLSQRRVAELSGLTHSAISTIEQDKVSPAISTLQKLLKVYGLSLSAFFAEPEAANEPKVVI
EAEDLIEIGSQGVSMKLVHNGNPTRNLAMMLETYQPGTTTGEKIKHQGEEIGTLLEGEIMLTINGQTHCLTAGQSYAINT
GIPHSFSNTSARVCRIVSAHTPTTF

Sequences:

>Translated_185_residues
MSEVSLAPGKRLSQIRLQLGLSQRRVAELSGLTHSAISTIEQDKVSPAISTLQKLLKVYGLSLSAFFAEPEAANEPKVVI
EAEDLIEIGSQGVSMKLVHNGNPTRNLAMMLETYQPGTTTGEKIKHQGEEIGTLLEGEIMLTINGQTHCLTAGQSYAINT
GIPHSFSNTSARVCRIVSAHTPTTF
>Mature_184_residues
SEVSLAPGKRLSQIRLQLGLSQRRVAELSGLTHSAISTIEQDKVSPAISTLQKLLKVYGLSLSAFFAEPEAANEPKVVIE
AEDLIEIGSQGVSMKLVHNGNPTRNLAMMLETYQPGTTTGEKIKHQGEEIGTLLEGEIMLTINGQTHCLTAGQSYAINTG
IPHSFSNTSARVCRIVSAHTPTTF

Specific function: Represses puuD [H]

COG id: COG1396

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=185, Percent_Identity=83.2432432432432, Blast_Score=318, Evalue=1e-88,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 19900; Mature: 19769

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVSLAPGKRLSQIRLQLGLSQRRVAELSGLTHSAISTIEQDKVSPAISTLQKLLKVYG
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHC
LSLSAFFAEPEAANEPKVVIEAEDLIEIGSQGVSMKLVHNGNPTRNLAMMLETYQPGTTT
CCCEEEECCCCCCCCCEEEEEHHHHHHHCCCCCEEEEEECCCCCHHEEEEEEECCCCCCC
GEKIKHQGEEIGTLLEGEIMLTINGQTHCLTAGQSYAINTGIPHSFSNTSARVCRIVSAH
HHHHHHHHHHHHHEECCEEEEEECCCEEEEECCCCEEEECCCCCCCCCCHHHHHHEECCC
TPTTF
CCCCC
>Mature Secondary Structure 
SEVSLAPGKRLSQIRLQLGLSQRRVAELSGLTHSAISTIEQDKVSPAISTLQKLLKVYG
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHC
LSLSAFFAEPEAANEPKVVIEAEDLIEIGSQGVSMKLVHNGNPTRNLAMMLETYQPGTTT
CCCEEEECCCCCCCCCEEEEEHHHHHHHCCCCCEEEEEECCCCCHHEEEEEEECCCCCCC
GEKIKHQGEEIGTLLEGEIMLTINGQTHCLTAGQSYAINTGIPHSFSNTSARVCRIVSAH
HHHHHHHHHHHHHEECCEEEEEECCCEEEEECCCCEEEECCCCCCCCCCHHHHHHEECCC
TPTTF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]