Click here to switch to the map view.

The map label for this gene is paaJ [H]

Identifier: 157371321

GI number: 157371321

Start: 3390149

End: 3391354

Strand: Direct

Name: paaJ [H]

Synonym: Spro_3081

Alternate gene names: 157371321

Gene position: 3390149-3391354 (Clockwise)

Preceding gene: 157371320

Following gene: 157371322

Centisome position: 62.22

GC content: 65.17

Gene sequence:

>1206_bases
ATGAGTCAGGCGTTTATCTGCGATGGCGTTCGCACGCCGATTGGCCGCTACGGCGGCGCATTGGCCAACGTGCGTGCCGA
TGATTTGGCCGCTCTGCCGCTGCGTGCCCTGTTAGCTCGCCACCCACAGGTGGACTGGTCATTGGTCGATGATGTGATCC
TCGGCTGCGCCAATCAGGCCGGGGAAGACAACCGCAATCTGGCCCGGATGGCAGTATTGCTGGCCGGCCTGCCGGTGAAC
GTTTCCGGCACTACCGTCAATCGCCTGTGCGGTTCGGGGCTGGACGCGCTGGCCATGGCGGCTCGCAGCATCAAGGCCGG
TGAAGCCGGGCTGGTGCTGGCCGGCGGCGCAGAATCAATGACCCGCGCCCCGCTGGTGATGGGCAAAGCCGACAGCGCTT
TCAGCCGTCAGGCGCAACTGTATGACACCACTCTGGGCTGGCGCTTTATCAATCCGCTGATGCAGGCGCAGTTCGGCACC
GACTCGATGCCGGAAACCGCCGAAAACGTGGCGGCGCAGTTCAACATCAGCCGCGCCGATCAGGACGCCTTCGCGCTGCG
CAGCCAGCAACGCGCCGCCCGGGCGCAAGAGTCAGGTTTACTGGCGCAGGAGATAGTGCCGGTCAGCCTCAGCGGTAAAA
AAGGCGCGGTGACGTTGTTCAGCCAGGACGAACACCCGCGCGCAGACACCCGGCTGGAACAATTGCAGGCGCTGAAAACG
CCGTTCCGCCAACCCGGTACCGTGACCGCCGGTAATGCCTCCGGCTTAAACGACGGCGCAGCGGCGCTGATTGTTGCCTC
CGAGGCAATCGCCGTCAGTCAGGGCCTCACCCCGCGGGCGCGTATCGTCGCCACCGCCACCTGCGGCGTCGAACCCGGTT
TGATGGGGATCGGCCCACTGCCGGCCACCCGCAAGGTACTGGAGTTAGCCGGGCTAAGCCTGGCGCAAATGGACGTGATC
GAACTGAATGAGGCCTTTGCCGCCCAGGCGTTGGCGGTACTGCGCCAGCTTGGCCTGCCGGACGACGCGCCGCAGGTGAA
TCCCAACGGTGGCGCTATTGCCCTTGGCCACCCGCTGGGGATGAGCGGTGCACGCCTGGCGCTGGCCGCCTTGTTTGAAC
TGGAACGGCGTTCCGGCCGCTACGCGCTTTGCACCATGTGCATCGGCGTCGGCCAAGGCATCGCCATGATCATTGAGCGA
GTTTGA

Upstream 100 bases:

>100_bases
GGCTGTATGACGTCGAAATCGTCAACCAACTGGGCAAGACCGTCGCCTGGTTCCGCGGGCGGGCGCATCGCCTCGGCCAT
GCCATTTTAGGGGAACAACC

Downstream 100 bases:

>100_bases
CCCCGAGGCTAATAATGATGACTACCAATCCACCGCCACTCGACGCCATTGAGTTCGCCTCGCGTGATGAAATTGAAGCG
CTGCAGCTGGAGCGTCTGAA

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSQAFICDGVRTPIGRYGGALANVRADDLAALPLRALLARHPQVDWSLVDDVILGCANQAGEDNRNLARMAVLLAGLPVN
VSGTTVNRLCGSGLDALAMAARSIKAGEAGLVLAGGAESMTRAPLVMGKADSAFSRQAQLYDTTLGWRFINPLMQAQFGT
DSMPETAENVAAQFNISRADQDAFALRSQQRAARAQESGLLAQEIVPVSLSGKKGAVTLFSQDEHPRADTRLEQLQALKT
PFRQPGTVTAGNASGLNDGAAALIVASEAIAVSQGLTPRARIVATATCGVEPGLMGIGPLPATRKVLELAGLSLAQMDVI
ELNEAFAAQALAVLRQLGLPDDAPQVNPNGGAIALGHPLGMSGARLALAALFELERRSGRYALCTMCIGVGQGIAMIIER
V

Sequences:

>Translated_401_residues
MSQAFICDGVRTPIGRYGGALANVRADDLAALPLRALLARHPQVDWSLVDDVILGCANQAGEDNRNLARMAVLLAGLPVN
VSGTTVNRLCGSGLDALAMAARSIKAGEAGLVLAGGAESMTRAPLVMGKADSAFSRQAQLYDTTLGWRFINPLMQAQFGT
DSMPETAENVAAQFNISRADQDAFALRSQQRAARAQESGLLAQEIVPVSLSGKKGAVTLFSQDEHPRADTRLEQLQALKT
PFRQPGTVTAGNASGLNDGAAALIVASEAIAVSQGLTPRARIVATATCGVEPGLMGIGPLPATRKVLELAGLSLAQMDVI
ELNEAFAAQALAVLRQLGLPDDAPQVNPNGGAIALGHPLGMSGARLALAALFELERRSGRYALCTMCIGVGQGIAMIIER
V
>Mature_400_residues
SQAFICDGVRTPIGRYGGALANVRADDLAALPLRALLARHPQVDWSLVDDVILGCANQAGEDNRNLARMAVLLAGLPVNV
SGTTVNRLCGSGLDALAMAARSIKAGEAGLVLAGGAESMTRAPLVMGKADSAFSRQAQLYDTTLGWRFINPLMQAQFGTD
SMPETAENVAAQFNISRADQDAFALRSQQRAARAQESGLLAQEIVPVSLSGKKGAVTLFSQDEHPRADTRLEQLQALKTP
FRQPGTVTAGNASGLNDGAAALIVASEAIAVSQGLTPRARIVATATCGVEPGLMGIGPLPATRKVLELAGLSLAQMDVIE
LNEAFAAQALAVLRQLGLPDDAPQVNPNGGAIALGHPLGMSGARLALAALFELERRSGRYALCTMCIGVGQGIAMIIERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=397, Percent_Identity=44.3324937027708, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=41.0579345088161, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI4501853, Length=404, Percent_Identity=39.6039603960396, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI4557237, Length=403, Percent_Identity=35.7320099255583, Blast_Score=221, Evalue=7e-58,
Organism=Homo sapiens, GI4504327, Length=426, Percent_Identity=34.5070422535211, Blast_Score=195, Evalue=6e-50,
Organism=Homo sapiens, GI194353979, Length=396, Percent_Identity=30.3030303030303, Blast_Score=135, Evalue=7e-32,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=76.8079800498753, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=49.8759305210918, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=44.4444444444444, Blast_Score=298, Evalue=6e-82,
Organism=Escherichia coli, GI87082165, Length=400, Percent_Identity=45.75, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1788683, Length=424, Percent_Identity=33.0188679245283, Blast_Score=170, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI133906874, Length=402, Percent_Identity=44.7761194029851, Blast_Score=314, Evalue=6e-86,
Organism=Caenorhabditis elegans, GI25147385, Length=405, Percent_Identity=36.0493827160494, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17535921, Length=407, Percent_Identity=33.9066339066339, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17551802, Length=436, Percent_Identity=32.1100917431193, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17535917, Length=401, Percent_Identity=29.6758104738155, Blast_Score=154, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6325229, Length=410, Percent_Identity=36.8292682926829, Blast_Score=220, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6322031, Length=404, Percent_Identity=36.6336633663366, Blast_Score=216, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=43.8902743142145, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI17648125, Length=407, Percent_Identity=39.8034398034398, Blast_Score=254, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24640423, Length=405, Percent_Identity=34.5679012345679, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI17137578, Length=425, Percent_Identity=32.4705882352941, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41723; Mature: 41591

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAFICDGVRTPIGRYGGALANVRADDLAALPLRALLARHPQVDWSLVDDVILGCANQA
CCCCEEECCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
GEDNRNLARMAVLLAGLPVNVSGTTVNRLCGSGLDALAMAARSIKAGEAGLVLAGGAESM
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCHH
TRAPLVMGKADSAFSRQAQLYDTTLGWRFINPLMQAQFGTDSMPETAENVAAQFNISRAD
HCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCC
QDAFALRSQQRAARAQESGLLAQEIVPVSLSGKKGAVTLFSQDEHPRADTRLEQLQALKT
HHHHHHHHHHHHHHHHHCCCHHHHEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHC
PFRQPGTVTAGNASGLNDGAAALIVASEAIAVSQGLTPRARIVATATCGVEPGLMGIGPL
CCCCCCCEECCCCCCCCCCCEEEEEEHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCC
PATRKVLELAGLSLAQMDVIELNEAFAAQALAVLRQLGLPDDAPQVNPNGGAIALGHPLG
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCC
MSGARLALAALFELERRSGRYALCTMCIGVGQGIAMIIERV
CCHHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure 
SQAFICDGVRTPIGRYGGALANVRADDLAALPLRALLARHPQVDWSLVDDVILGCANQA
CCCEEECCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
GEDNRNLARMAVLLAGLPVNVSGTTVNRLCGSGLDALAMAARSIKAGEAGLVLAGGAESM
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCHH
TRAPLVMGKADSAFSRQAQLYDTTLGWRFINPLMQAQFGTDSMPETAENVAAQFNISRAD
HCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCC
QDAFALRSQQRAARAQESGLLAQEIVPVSLSGKKGAVTLFSQDEHPRADTRLEQLQALKT
HHHHHHHHHHHHHHHHHCCCHHHHEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHC
PFRQPGTVTAGNASGLNDGAAALIVASEAIAVSQGLTPRARIVATATCGVEPGLMGIGPL
CCCCCCCEECCCCCCCCCCCEEEEEEHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCC
PATRKVLELAGLSLAQMDVIELNEAFAAQALAVLRQLGLPDDAPQVNPNGGAIALGHPLG
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCC
MSGARLALAALFELERRSGRYALCTMCIGVGQGIAMIIERV
CCHHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 10766858 [H]