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The map label for this gene is paaI [H]

Identifier: 157371320

GI number: 157371320

Start: 3389712

End: 3390152

Strand: Direct

Name: paaI [H]

Synonym: Spro_3080

Alternate gene names: 157371320

Gene position: 3389712-3390152 (Clockwise)

Preceding gene: 157371319

Following gene: 157371321

Centisome position: 62.21

GC content: 63.04

Gene sequence:

>441_bases
ATGAACGCCAATACGCCACAGGCACTGGCGCAACGCTGCGCCGAACAGATGTTTCAACAGGATACCTGTGCCCAGGCGAT
GGGGATGCGGGTGGATGCGGTTGATGCCGGTTTCGCCCGGGTCAGCATGACCGTCGGTCCGCAGATGCTCAACGGCCACC
AGACCTGCCACGGCGGCCAACTTTTCAGCCTGGCGGACACCGCCTTCGCCTATGCCTGCAACAGTCAGGGGCTGGCGGCA
GTGGCCTCTGGCTGCAATATCGAGTTTATCCGCCCGGCTCTGGCCGGCGATCGCCTGACTGCCAGCGCCGAGGTACGCCA
TCAGGGTAAAACCACCGGGCTGTATGACGTCGAAATCGTCAACCAACTGGGCAAGACCGTCGCCTGGTTCCGCGGGCGGG
CGCATCGCCTCGGCCATGCCATTTTAGGGGAACAACCATGA

Upstream 100 bases:

>100_bases
TCACCACGCTGGAAAACCTGCAACTCTACTACGGTGAGCCACGTTATCGCCCAATGCCGCTGCTGCGTCATTACGCATCC
CTCTCTTCAGGAGCCCAACC

Downstream 100 bases:

>100_bases
GTCAGGCGTTTATCTGCGATGGCGTTCGCACGCCGATTGGCCGCTACGGCGGCGCATTGGCCAACGTGCGTGCCGATGAT
TTGGCCGCTCTGCCGCTGCG

Product: phenylacetic acid degradation protein PaaD

Products: NA

Alternate protein names: Phenylacetic acid degradation protein PaaI [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MNANTPQALAQRCAEQMFQQDTCAQAMGMRVDAVDAGFARVSMTVGPQMLNGHQTCHGGQLFSLADTAFAYACNSQGLAA
VASGCNIEFIRPALAGDRLTASAEVRHQGKTTGLYDVEIVNQLGKTVAWFRGRAHRLGHAILGEQP

Sequences:

>Translated_146_residues
MNANTPQALAQRCAEQMFQQDTCAQAMGMRVDAVDAGFARVSMTVGPQMLNGHQTCHGGQLFSLADTAFAYACNSQGLAA
VASGCNIEFIRPALAGDRLTASAEVRHQGKTTGLYDVEIVNQLGKTVAWFRGRAHRLGHAILGEQP
>Mature_146_residues
MNANTPQALAQRCAEQMFQQDTCAQAMGMRVDAVDAGFARVSMTVGPQMLNGHQTCHGGQLFSLADTAFAYACNSQGLAA
VASGCNIEFIRPALAGDRLTASAEVRHQGKTTGLYDVEIVNQLGKTVAWFRGRAHRLGHAILGEQP

Specific function: Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA [H]

COG id: COG2050

COG function: function code Q; Uncharacterized protein, possibly involved in aromatic compounds catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase PaaI family [H]

Homologues:

Organism=Escherichia coli, GI1787662, Length=131, Percent_Identity=68.7022900763359, Blast_Score=195, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011973
- InterPro:   IPR003736
- InterPro:   IPR006683 [H]

Pfam domain/function: PF03061 4HBT [H]

EC number: NA

Molecular weight: Translated: 15565; Mature: 15565

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNANTPQALAQRCAEQMFQQDTCAQAMGMRVDAVDAGFARVSMTVGPQMLNGHQTCHGGQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHCCCCCCCCCE
LFSLADTAFAYACNSQGLAAVASGCNIEFIRPALAGDRLTASAEVRHQGKTTGLYDVEIV
EHHHHHHHHHHHCCCCCHHHHHCCCCEEEECHHHCCCCCCCHHHHHHCCCCCCCHHHHHH
NQLGKTVAWFRGRAHRLGHAILGEQP
HHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNANTPQALAQRCAEQMFQQDTCAQAMGMRVDAVDAGFARVSMTVGPQMLNGHQTCHGGQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHCCCCCCCCCE
LFSLADTAFAYACNSQGLAAVASGCNIEFIRPALAGDRLTASAEVRHQGKTTGLYDVEIV
EHHHHHHHHHHHCCCCCHHHHHCCCCEEEECHHHCCCCCCCHHHHHHCCCCCCCHHHHHH
NQLGKTVAWFRGRAHRLGHAILGEQP
HHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]