BacMap

Definition	Serratia proteamaculans 568 chromosome, complete genome.
Accession	NC_009832
Length	5,448,853

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The map label for this gene is paaF [H]

Identifier: 157371317

GI number: 157371317

Start: 3386624

End: 3387397

Strand: Direct

Name: paaF [H]

Synonym: Spro_3077

Alternate gene names: 157371317

Gene position: 3386624-3387397 (Clockwise)

Preceding gene: 157371316

Following gene: 157371318

Centisome position: 62.15

GC content: 63.31

Gene sequence:

>774_bases
ATGGAAACCCCTTTTGTCCTGCGACAGCAACACCAGCGAGTGGTGACACTGACACTGCATCGCCCAGAGGCGCGCAACGC
CCTGAGCACCCCGTGCCTGGAACAGTTGGTTTATTGGCTGGAACTGGCCGACGCCGATGCCACCGTTGGCGCAGTGGTGA
TTACCGGCGCGCCCCGCTTCTTTGCCGCCGGTGCCGACCTGCGCGAGCTGCAAAAGCAAGACTTGCCCGCCACGTTGACG
GATCGCCGTCCGCTGTTGTGGCAGCGCCTGGCCCAGTTCAGCAAACCGCTGCTGGCGGCGGTCAACGGCTATGCGCTGGG
TGCCGGTTGTGAATTGGCATTGGCCTGCGACATCGTTATCTGCGGCGAAAGCGCCCGTTTTGGCCTGCCGGAAATCACCC
TCGGCCTGATGCCAGGCGCCGGGGGTACCCAGCGTTTGATCCGCTGCGTCGGCAAGTCTCGCGCCAGTCAGATGGTGCTG
ACCGGTGAAGCCATCAACGCCAGTTGCGCACTGCAAAGTGGCCTGGTCAGTGAAGTCTGTGTCGATGCGCTGACGCTGGA
GCGCACGCAACAGGTTGCCGAACGCATCAGCCTGCAGGCGCCGCTGGCATTACGCGCCGCCAAGCAGGCATTGAAACAGG
CTGAAGAAACCGGCCTTAGCCAGGGGCTGGCCATCGAGCGCCAGCAGTTTGTCACCCTGGCCGCCACCGACGATCGCCGT
GAAGGCATCGCCGCCTTCTTCGAAAAACGTACGCCAAACTATCAGGGGCGCTGA

Upstream 100 bases:

>100_bases
CCTGGAGCCGGATCAACTGGCGGCGGGTTACGTATTGAGTTGTCAGTCGTGGCCGCGTGGCGACGGTGTGATATTGGACT
TTGACGTATAGGAGCCGCCG

Downstream 100 bases:

>100_bases
TTTATGGATAACGCATTGATTCTCACCCATCTGGAAGCCGGGGTGTTGACCCTGACTCTTAACCGGCCAGACCGGCTCAA
CAGTTTTAACGACGAGATGC

Product: enoyl-CoA hydratase-isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
METPFVLRQQHQRVVTLTLHRPEARNALSTPCLEQLVYWLELADADATVGAVVITGAPRFFAAGADLRELQKQDLPATLT
DRRPLLWQRLAQFSKPLLAAVNGYALGAGCELALACDIVICGESARFGLPEITLGLMPGAGGTQRLIRCVGKSRASQMVL
TGEAINASCALQSGLVSEVCVDALTLERTQQVAERISLQAPLALRAAKQALKQAEETGLSQGLAIERQQFVTLAATDDRR
EGIAAFFEKRTPNYQGR

Sequences:

>Translated_257_residues
METPFVLRQQHQRVVTLTLHRPEARNALSTPCLEQLVYWLELADADATVGAVVITGAPRFFAAGADLRELQKQDLPATLT
DRRPLLWQRLAQFSKPLLAAVNGYALGAGCELALACDIVICGESARFGLPEITLGLMPGAGGTQRLIRCVGKSRASQMVL
TGEAINASCALQSGLVSEVCVDALTLERTQQVAERISLQAPLALRAAKQALKQAEETGLSQGLAIERQQFVTLAATDDRR
EGIAAFFEKRTPNYQGR
>Mature_257_residues
METPFVLRQQHQRVVTLTLHRPEARNALSTPCLEQLVYWLELADADATVGAVVITGAPRFFAAGADLRELQKQDLPATLT
DRRPLLWQRLAQFSKPLLAAVNGYALGAGCELALACDIVICGESARFGLPEITLGLMPGAGGTQRLIRCVGKSRASQMVL
TGEAINASCALQSGLVSEVCVDALTLERTQQVAERISLQAPLALRAAKQALKQAEETGLSQGLAIERQQFVTLAATDDRR
EGIAAFFEKRTPNYQGR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=44.3548387096774, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI4502327, Length=264, Percent_Identity=35.6060606060606, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI150378541, Length=252, Percent_Identity=32.9365079365079, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI31542718, Length=244, Percent_Identity=32.3770491803279, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI68989263, Length=195, Percent_Identity=37.9487179487179, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI70995211, Length=257, Percent_Identity=29.1828793774319, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI20127408, Length=174, Percent_Identity=34.4827586206897, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI157694516, Length=246, Percent_Identity=28.4552845528455, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI213417737, Length=246, Percent_Identity=28.4552845528455, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI4757968, Length=187, Percent_Identity=31.0160427807487, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI49355787, Length=187, Percent_Identity=31.0160427807487, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI45643119, Length=214, Percent_Identity=25.7009345794392, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI260275230, Length=214, Percent_Identity=25.7009345794392, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI25453481, Length=195, Percent_Identity=30.2564102564103, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI51538573, Length=195, Percent_Identity=30.2564102564103, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI260274832, Length=214, Percent_Identity=25.7009345794392, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4757966, Length=195, Percent_Identity=30.2564102564103, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI51538581, Length=195, Percent_Identity=30.2564102564103, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI157694520, Length=201, Percent_Identity=28.3582089552239, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI261878539, Length=101, Percent_Identity=43.5643564356436, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI221136756, Length=218, Percent_Identity=28.4403669724771, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI221136753, Length=218, Percent_Identity=28.4403669724771, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI221307494, Length=218, Percent_Identity=28.4403669724771, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI37594469, Length=192, Percent_Identity=26.5625, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI37594471, Length=192, Percent_Identity=26.5625, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI62530384, Length=246, Percent_Identity=27.6422764227642, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787659, Length=257, Percent_Identity=63.0350194552529, Blast_Score=313, Evalue=5e-87,
Organism=Escherichia coli, GI221142681, Length=256, Percent_Identity=32.8125, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=36.2934362934363, Blast_Score=123, Evalue=9e-30,
Organism=Escherichia coli, GI87082183, Length=254, Percent_Identity=29.1338582677165, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788682, Length=203, Percent_Identity=33.4975369458128, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788597, Length=246, Percent_Identity=27.2357723577236, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1790281, Length=124, Percent_Identity=39.5161290322581, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=248, Percent_Identity=43.1451612903226, Blast_Score=213, Evalue=8e-56,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=42.798353909465, Blast_Score=196, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI17540714, Length=228, Percent_Identity=31.5789473684211, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17558304, Length=196, Percent_Identity=33.1632653061224, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17560910, Length=209, Percent_Identity=29.1866028708134, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=246, Percent_Identity=26.4227642276423, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=246, Percent_Identity=27.6422764227642, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=249, Percent_Identity=28.9156626506024, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=25.78125, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=267, Percent_Identity=23.9700374531835, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI25144276, Length=212, Percent_Identity=30.6603773584906, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17508951, Length=212, Percent_Identity=30.6603773584906, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17508953, Length=220, Percent_Identity=30.4545454545455, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320241, Length=204, Percent_Identity=30.3921568627451, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=42.8571428571429, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=42.8571428571429, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=35.8267716535433, Blast_Score=138, Evalue=4e-33,
Organism=Drosophila melanogaster, GI19922422, Length=262, Percent_Identity=28.6259541984733, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19920382, Length=254, Percent_Identity=28.740157480315, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24654903, Length=178, Percent_Identity=28.0898876404494, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21357171, Length=178, Percent_Identity=29.7752808988764, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24650670, Length=247, Percent_Identity=26.3157894736842, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19921000, Length=192, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24583077, Length=192, Percent_Identity=32.8125, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583079, Length=192, Percent_Identity=32.8125, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583165, Length=243, Percent_Identity=23.0452674897119, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27796; Mature: 27796

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METPFVLRQQHQRVVTLTLHRPEARNALSTPCLEQLVYWLELADADATVGAVVITGAPRF
CCCCHHHHHCCCEEEEEEEECCHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHH
FAAGADLRELQKQDLPATLTDRRPLLWQRLAQFSKPLLAAVNGYALGAGCELALACDIVI
HCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCEEECCCCEEEEEEEEEE
CGESARFGLPEITLGLMPGAGGTQRLIRCVGKSRASQMVLTGEAINASCALQSGLVSEVC
ECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHH
VDALTLERTQQVAERISLQAPLALRAAKQALKQAEETGLSQGLAIERQQFVTLAATDDRR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEECHHHEEEEEECCCHH
EGIAAFFEKRTPNYQGR
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure
METPFVLRQQHQRVVTLTLHRPEARNALSTPCLEQLVYWLELADADATVGAVVITGAPRF
CCCCHHHHHCCCEEEEEEEECCHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHH
FAAGADLRELQKQDLPATLTDRRPLLWQRLAQFSKPLLAAVNGYALGAGCELALACDIVI
HCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCEEECCCCEEEEEEEEEE
CGESARFGLPEITLGLMPGAGGTQRLIRCVGKSRASQMVLTGEAINASCALQSGLVSEVC
ECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHH
VDALTLERTQQVAERISLQAPLALRAAKQALKQAEETGLSQGLAIERQQFVTLAATDDRR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEECHHHEEEEEECCCHH
EGIAAFFEKRTPNYQGR
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]