Click here to switch to the map view.

The map label for this gene is astE

Identifier: 157371080

GI number: 157371080

Start: 3116001

End: 3116984

Strand: Reverse

Name: astE

Synonym: Spro_2840

Alternate gene names: 157371080

Gene position: 3116984-3116001 (Counterclockwise)

Preceding gene: 157371081

Following gene: 157371079

Centisome position: 57.2

GC content: 57.93

Gene sequence:

>984_bases
ATGTTTGATTTGTTGGCCCTGACGCTGGCGGGGCAGGTTCCCGCTGAGCAACAGGGCGAAAATGCGAATTTGCACTGGCG
TTGGCTGACGGAAGGCCTGCTGGAAATGACCCCGAAGCCAGGTTACCGTCAGGCGGTGGTACTTTCTGCGGGTATCCACG
GCAATGAAACCGCCCCTATTGAGCTGTTGAATCAGTTGGTCAAAGACCTGCTCAACGGCGATCGTCCCCTGGCGGTTCGG
CTGCTGGTGGTGTTGGGTAACCCGGCGGCGATGCGTGCCGGAAAGCGTTATCTGCACAGTGACATGAACCGGATGTTTGG
CGGACGCTACCGCGATTTTGACCACAGCGGTGAAACCGCGCGGGCGCAGCAACTGGAGCAGGTTATCGCGGAGTTTTTCG
CCGATGAGGGCGCTGCACGTTTCCATTATGATCTGCATACGGCAATCCGTGATTCGCGTCTGCCGCGCTTTGGTATTCTG
CCTTTCCAGACGCGACCTTACAGTGCAGAGATGCTGGCCTTGCTGGACGCAGCCGATTTGGATGCATTGGTGATCCACAG
CGCACCGGGCGGTACCTTCAGCCATTTCACCAGCGAACATGCCGGCGCCGCCAGCTGCACGCTGGAGTTGGGTAAGGCGC
GGCCGTTCGGCGTTAACGATCTGCAGCAGTTTGCCGCCATTAACCGTGCACTGCAGGCGGCGGTGAGCGGGGAGCCTTTG
CCGTTGCGGTCAGGAAGTGAACTGCGAATATTCCGCGTTGAACGTTCGCTGATTAAGCGTAGTGAAGAGTTCAGGTTGCA
TCTCAGTGATGATACCGCCAACTTTACCGAGCTGAAACAGGGAACGCTGCTGTGTGACCAGCCTGGCGAAGAGTATCGGG
TTCAGCATGTCAGCGAATGGATATTGTTCCCTAATCCTGGTGTAGCGCTTGGGTTGCGTGCCGGCATGGTGCTGGTTGAA
GCGCCGCGCAGCACGCTATATTGA

Upstream 100 bases:

>100_bases
ACCCGCAACTGTTGCGTGAAGGGCGTGAAGCGCTGGACTCCCTGACAACCATCCTCGGGTTGGGTTCGGTTTATCCTTTC
CAACAATAGACGGAGCCACC

Downstream 100 bases:

>100_bases
AAGAGCGCCTTATAAGGCGGTTTTATTCTTATGCTATTTAAGGTTGTAGCGATGTTGGCTGCAACCTCAAATAATATCGG
TATATAATGCTGCTTATTTC

Product: succinylglutamate desuccinylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MFDLLALTLAGQVPAEQQGENANLHWRWLTEGLLEMTPKPGYRQAVVLSAGIHGNETAPIELLNQLVKDLLNGDRPLAVR
LLVVLGNPAAMRAGKRYLHSDMNRMFGGRYRDFDHSGETARAQQLEQVIAEFFADEGAARFHYDLHTAIRDSRLPRFGIL
PFQTRPYSAEMLALLDAADLDALVIHSAPGGTFSHFTSEHAGAASCTLELGKARPFGVNDLQQFAAINRALQAAVSGEPL
PLRSGSELRIFRVERSLIKRSEEFRLHLSDDTANFTELKQGTLLCDQPGEEYRVQHVSEWILFPNPGVALGLRAGMVLVE
APRSTLY

Sequences:

>Translated_327_residues
MFDLLALTLAGQVPAEQQGENANLHWRWLTEGLLEMTPKPGYRQAVVLSAGIHGNETAPIELLNQLVKDLLNGDRPLAVR
LLVVLGNPAAMRAGKRYLHSDMNRMFGGRYRDFDHSGETARAQQLEQVIAEFFADEGAARFHYDLHTAIRDSRLPRFGIL
PFQTRPYSAEMLALLDAADLDALVIHSAPGGTFSHFTSEHAGAASCTLELGKARPFGVNDLQQFAAINRALQAAVSGEPL
PLRSGSELRIFRVERSLIKRSEEFRLHLSDDTANFTELKQGTLLCDQPGEEYRVQHVSEWILFPNPGVALGLRAGMVLVE
APRSTLY
>Mature_327_residues
MFDLLALTLAGQVPAEQQGENANLHWRWLTEGLLEMTPKPGYRQAVVLSAGIHGNETAPIELLNQLVKDLLNGDRPLAVR
LLVVLGNPAAMRAGKRYLHSDMNRMFGGRYRDFDHSGETARAQQLEQVIAEFFADEGAARFHYDLHTAIRDSRLPRFGIL
PFQTRPYSAEMLALLDAADLDALVIHSAPGGTFSHFTSEHAGAASCTLELGKARPFGVNDLQQFAAINRALQAAVSGEPL
PLRSGSELRIFRVERSLIKRSEEFRLHLSDDTANFTELKQGTLLCDQPGEEYRVQHVSEWILFPNPGVALGLRAGMVLVE
APRSTLY

Specific function: Transforms N(2)-succinylglutamate into succinate and glutamate

COG id: COG2988

COG function: function code E; Succinylglutamate desuccinylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily

Homologues:

Organism=Escherichia coli, GI1788040, Length=320, Percent_Identity=51.25, Blast_Score=320, Evalue=8e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASTE_SERP5 (A8GFQ1)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479069.1
- ProteinModelPortal:   A8GFQ1
- SMR:   A8GFQ1
- STRING:   A8GFQ1
- GeneID:   5603098
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_2840
- eggNOG:   COG2988
- HOGENOM:   HBG298271
- OMA:   RWRWLGH
- ProtClustDB:   PRK05324
- BioCyc:   SPRO399741:SPRO_2840-MONOMER
- HAMAP:   MF_00767
- InterPro:   IPR007036
- InterPro:   IPR016681
- PIRSF:   PIRSF017020
- TIGRFAMs:   TIGR03242

Pfam domain/function: PF04952 AstE_AspA

EC number: =3.5.1.96

Molecular weight: Translated: 36208; Mature: 36208

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: ACT_SITE 209-209

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDLLALTLAGQVPAEQQGENANLHWRWLTEGLLEMTPKPGYRQAVVLSAGIHGNETAPI
CCHHHEEHHHCCCCCHHCCCCCCEEEEEHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHH
ELLNQLVKDLLNGDRPLAVRLLVVLGNPAAMRAGKRYLHSDMNRMFGGRYRDFDHSGETA
HHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
RAQQLEQVIAEFFADEGAARFHYDLHTAIRDSRLPRFGILPFQTRPYSAEMLALLDAADL
HHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
DALVIHSAPGGTFSHFTSEHAGAASCTLELGKARPFGVNDLQQFAAINRALQAAVSGEPL
CEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PLRSGSELRIFRVERSLIKRSEEFRLHLSDDTANFTELKQGTLLCDQPGEEYRVQHVSEW
CCCCCCCEEEEHHHHHHHCCCCCEEEEECCCCCCHHHHCCCCEEECCCCHHHHHHHHHHE
ILFPNPGVALGLRAGMVLVEAPRSTLY
EEECCCCEEEEHHCCEEEEECCHHCCC
>Mature Secondary Structure
MFDLLALTLAGQVPAEQQGENANLHWRWLTEGLLEMTPKPGYRQAVVLSAGIHGNETAPI
CCHHHEEHHHCCCCCHHCCCCCCEEEEEHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHH
ELLNQLVKDLLNGDRPLAVRLLVVLGNPAAMRAGKRYLHSDMNRMFGGRYRDFDHSGETA
HHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
RAQQLEQVIAEFFADEGAARFHYDLHTAIRDSRLPRFGILPFQTRPYSAEMLALLDAADL
HHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
DALVIHSAPGGTFSHFTSEHAGAASCTLELGKARPFGVNDLQQFAAINRALQAAVSGEPL
CEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PLRSGSELRIFRVERSLIKRSEEFRLHLSDDTANFTELKQGTLLCDQPGEEYRVQHVSEW
CCCCCCCEEEEHHHHHHHCCCCCEEEEECCCCCCHHHHCCCCEEECCCCHHHHHHHHHHE
ILFPNPGVALGLRAGMVLVEAPRSTLY
EEECCCCEEEEHHCCEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA