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The map label for this gene is purT [H]

Identifier: 157371045

GI number: 157371045

Start: 3083632

End: 3084810

Strand: Direct

Name: purT [H]

Synonym: Spro_2805

Alternate gene names: 157371045

Gene position: 3083632-3084810 (Clockwise)

Preceding gene: 157371040

Following gene: 157371048

Centisome position: 56.59

GC content: 59.29

Gene sequence:

>1179_bases
ATGTTAACTATTGGAACCGCCCTGCGCCCGTCCGCCACCCGCGTTATGTTGCTCGGCTCCGGCGAGCTGGGTAAAGAAGT
TGCTATCGAATGCCAGCGTTTGGGCCTCGAAGTGATCGCCGTAGATCGCTATGCCGATGCCCCTGCCATGCATGTGGCGC
ACCGCAGCCACGTGATTAACATGCTCGATGGCAACGCGCTTAAAGCCGTGATTGAGCAGGAACGTCCTGATTATATCGTG
CCGGAGATCGAGGCCATCGCCACCAGCATGCTGGTTGAACTGGAACAGCAAGGACACCGCGTGGTGCCCTGCGCCGAGGC
AACGCGCCTGACGATGAACCGCGAAGGCATCCGCCGTCTGGCGGCGGAAACCCTTGGCCTGCCGACCTCCAGTTACCGTT
TTGCCGACGGCGAAACCGCTTTCCGCCAGGCGGTCGACGAAATCGGCTACCCGTGCATTATCAAGCCGGTGATGAGCTCT
TCCGGCAAGGGCCAAAGTCTGATCCGCACCCCGGACCAGCTGAAAACCGCCTGGGATTATGCCCAGCAAGGCGGTCGTGC
CGGTGGTGGCCGGGTGATTGTTGAAGGGCTGGTAAAATTTGATTTTGAAATCACCCTGCTGACCATCAGCGCTGTCGACG
GTGTGCATTTCTGTGCGCCGATCGGCCATCGCCAGGAGGACGGCGATTACCGCGAATCGTGGCAACCGCAGCAGATGAGT
GACCTGGCGCTAAGCCGCGCACAGGCGATTGCAGAAAATGTGGTGAAGGCGCTGGGCGGTTTTGGCCTGTTCGGCGTTGA
ATTGTTTGTTTGTGGCGATGAAGTGATCTTCAGCGAGGTTTCACCGCGCCCGCACGATACCGGCATGGTGACTCTGATTT
CACAAGATCTGTCCGAATTTGCGCTACACGTTCGCGCTTTCCTCGGTCTGCCGATTGGCGCCATTCGCCAGTTTGGCCCT
TCTGCTTCGGCGGTGATCCTGCCACAGCTAATCAGCGGTGACCTGCGCTTTAGTGGGCTGGATCGCGCGCTGGTCGGCCA
CAATCAACTGCGTTTGTTCGGCAAGCCGGAAATTAACGGTCAACGTCGGATGGGGGTCGCGCTGGCTACGGCAGAAAATG
TAGCGCTGGCGGTGGAATCGGCCAAACGGGCCGCAGCTGCGGTGGTGATTGAAGGTTAA

Upstream 100 bases:

>100_bases
GGCAGTTCAATAGCCTCCGGCTGCATACGCAAACGTTTTCGTATATACTGCCGGGCCATTATCCGACCGCATTACACTCC
ACAGATCCAGGTACGAGACT

Downstream 100 bases:

>100_bases
TGCTTTGGGGCCGGTTTGCCGGCCCCTACAACATCAATAGTTTTCGTCTTCTTCCGAGTCATCATCAGCCTGGTCATCCG
GCCGTTCCTGATACTCTTCA

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MLTIGTALRPSATRVMLLGSGELGKEVAIECQRLGLEVIAVDRYADAPAMHVAHRSHVINMLDGNALKAVIEQERPDYIV
PEIEAIATSMLVELEQQGHRVVPCAEATRLTMNREGIRRLAAETLGLPTSSYRFADGETAFRQAVDEIGYPCIIKPVMSS
SGKGQSLIRTPDQLKTAWDYAQQGGRAGGGRVIVEGLVKFDFEITLLTISAVDGVHFCAPIGHRQEDGDYRESWQPQQMS
DLALSRAQAIAENVVKALGGFGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPIGAIRQFGP
SASAVILPQLISGDLRFSGLDRALVGHNQLRLFGKPEINGQRRMGVALATAENVALAVESAKRAAAAVVIEG

Sequences:

>Translated_392_residues
MLTIGTALRPSATRVMLLGSGELGKEVAIECQRLGLEVIAVDRYADAPAMHVAHRSHVINMLDGNALKAVIEQERPDYIV
PEIEAIATSMLVELEQQGHRVVPCAEATRLTMNREGIRRLAAETLGLPTSSYRFADGETAFRQAVDEIGYPCIIKPVMSS
SGKGQSLIRTPDQLKTAWDYAQQGGRAGGGRVIVEGLVKFDFEITLLTISAVDGVHFCAPIGHRQEDGDYRESWQPQQMS
DLALSRAQAIAENVVKALGGFGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPIGAIRQFGP
SASAVILPQLISGDLRFSGLDRALVGHNQLRLFGKPEINGQRRMGVALATAENVALAVESAKRAAAAVVIEG
>Mature_392_residues
MLTIGTALRPSATRVMLLGSGELGKEVAIECQRLGLEVIAVDRYADAPAMHVAHRSHVINMLDGNALKAVIEQERPDYIV
PEIEAIATSMLVELEQQGHRVVPCAEATRLTMNREGIRRLAAETLGLPTSSYRFADGETAFRQAVDEIGYPCIIKPVMSS
SGKGQSLIRTPDQLKTAWDYAQQGGRAGGGRVIVEGLVKFDFEITLLTISAVDGVHFCAPIGHRQEDGDYRESWQPQQMS
DLALSRAQAIAENVVKALGGFGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPIGAIRQFGP
SASAVILPQLISGDLRFSGLDRALVGHNQLRLFGKPEINGQRRMGVALATAENVALAVESAKRAAAAVVIEG

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=392, Percent_Identity=78.5714285714286, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI1786733, Length=245, Percent_Identity=25.7142857142857, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324702, Length=367, Percent_Identity=23.433242506812, Blast_Score=103, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42204; Mature: 42204

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTIGTALRPSATRVMLLGSGELGKEVAIECQRLGLEVIAVDRYADAPAMHVAHRSHVIN
CEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHEEE
MLDGNALKAVIEQERPDYIVPEIEAIATSMLVELEQQGHRVVPCAEATRLTMNREGIRRL
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCHHHHHHCCHHHHHHH
AAETLGLPTSSYRFADGETAFRQAVDEIGYPCIIKPVMSSSGKGQSLIRTPDQLKTAWDY
HHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCCCCHHHHHHHHHH
AQQGGRAGGGRVIVEGLVKFDFEITLLTISAVDGVHFCAPIGHRQEDGDYRESWQPQQMS
HHCCCCCCCCHHHEEEHHHCCEEEEEEEEEECCCCCEECCCCCCCCCCCHHHCCCCHHHH
DLALSRAQAIAENVVKALGGFGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEF
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHECCCCCCCCCCCEEEEHHHHHHHH
ALHVRAFLGLPIGAIRQFGPSASAVILPQLISGDLRFSGLDRALVGHNQLRLFGKPEING
HHHHHHHHCCCHHHHHHCCCCCCCEEHHHHHCCCCEECCCCHHHHCCCEEEEECCCCCCC
QRRMGVALATAENVALAVESAKRAAAAVVIEG
CCCCEEEEEECCCEEEEHHHHCCCEEEEEEEC
>Mature Secondary Structure
MLTIGTALRPSATRVMLLGSGELGKEVAIECQRLGLEVIAVDRYADAPAMHVAHRSHVIN
CEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHEEE
MLDGNALKAVIEQERPDYIVPEIEAIATSMLVELEQQGHRVVPCAEATRLTMNREGIRRL
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCHHHHHHCCHHHHHHH
AAETLGLPTSSYRFADGETAFRQAVDEIGYPCIIKPVMSSSGKGQSLIRTPDQLKTAWDY
HHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCCCCHHHHHHHHHH
AQQGGRAGGGRVIVEGLVKFDFEITLLTISAVDGVHFCAPIGHRQEDGDYRESWQPQQMS
HHCCCCCCCCHHHEEEHHHCCEEEEEEEEEECCCCCEECCCCCCCCCCCHHHCCCCHHHH
DLALSRAQAIAENVVKALGGFGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEF
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHECCCCCCCCCCCEEEEHHHHHHHH
ALHVRAFLGLPIGAIRQFGPSASAVILPQLISGDLRFSGLDRALVGHNQLRLFGKPEING
HHHHHHHHCCCHHHHHHCCCCCCCEEHHHHHCCCCEECCCCHHHHCCCEEEEECCCCCCC
QRRMGVALATAENVALAVESAKRAAAAVVIEG
CCCCEEEEEECCCEEEEHHHHCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA