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The map label for this gene is aspS [H]

Identifier: 157371021

GI number: 157371021

Start: 3060447

End: 3062228

Strand: Reverse

Name: aspS [H]

Synonym: Spro_2781

Alternate gene names: 157371021

Gene position: 3062228-3060447 (Counterclockwise)

Preceding gene: 157371022

Following gene: 157371020

Centisome position: 56.2

GC content: 56.85

Gene sequence:

>1782_bases
ATGCGTACTGAATATTGCGGGCAGTTGAATCTGTCTCACGTGGGCCAGGAAGTCACTTTGTGTGGTTGGGTCAACCGTCG
TCGCGATCTGGGCGGCCTGATCTTTATTGATATGCGCGACCGCGAAGGCATCGTGCAGGTGTTTTTCGATCCGGACCAGA
AGGCCGCGTTCGATAAGGCGTACGACCTGCGCAATGAATTCTGCATCCAGATCGTCGGCACCGTGCGTGCCCGTCCGGAC
AGCCAGATCAATAAAGATATGGCCACCGGCGAAGTGGAAGTGTTCGCCCACGCTCTGGAGATCATCAACCGTTCCGAGCC
TCTGCCGCTGGACTCCAACCAGGTCAACAGCGAAGAAGCGCGCCTGAAATATCGCTACCTGGATCTGCGTCGTCCGGAAA
TGGCCGATCGCTTGAAAACCCGCGCCAAAATCACCAGTTTTGTGCGTCGCTTTATGGACAGCCACGGCTTCCTCGATATC
GAAACGCCGATGTTGACCAAAGCCACGCCGGAAGGTGCCCGTGACTACCTGGTACCGAGCCGGGTACATAAAGGCAAATT
CTATGCGTTACCGCAGTCACCACAGCTGTTCAAACAGTTGCTGATGATGTCCGGTTTCGATCGCTACTATCAAATCGTTA
AATGCTTCCGCGACGAAGACCTGCGTGCTGACCGTCAGCCTGAATTTACCCAGATCGACGTGGAAACCTCTTTCATGACC
GCCGAACAGGTGCGTGAAGTGATGGAGAAACTGGCGCGTGAACTGTGGTTGGACGTGAAGAACGTAGATCTGGGTGATTT
CCCGATCATGACCTTCGAAGAAGCGATGCGCCGTTACGGCTCGGACAAACCGGACCTGCGTAACCCATTGGAACTGGTGG
ACGTTGCCGACCTGGTCAAAGACGTCGAGTTCAAAGTGTTCTCCGGCCCGGCTAACGATGCCAAAGGCCGCGTAGCCGCG
ATTTGCGTTCCGGGCGGCGCGCAACTGACGCGTAAACTGATCGACGAGTACGGCGCTTTCGTTAACATCTATGGTGCCAA
AGGCCTGGCCTGGCTGAAGGTCAATGACCGTGCAGCCGGCATGGAAGGCGTACAGAGCCCGATCGCCAAATTCCTCAGCG
CAGAAGTGCTGGAAGCGATCCTGGCGCGCACTAACGCGCAGTCAGGCGACATTCTGTTCTTCGGCGCCGACAGCTTCAAA
ATTGTCACCGATGCGATGGGCGCACTGCGTCTGAAACTGGGCCGCGATCTGGAGCTGACTAAACTGGACAGCTGGGCACC
GCTATGGGTGGTTGACTTCCCAATGTTCGAAGAGGACGGTGAAGGCGGCCTGGCAGCGATGCACCATCCGTTCACTGCTC
CACGCGACATGAGCCCGGAAGAGCTGGCTGGCACGCCAACTAACGCTATTGCCAACGCTTACGACATGGTTATCAATGGC
TATGAAGTGGGCGGCGGTTCAGTGCGTATTCACCGTAGTGAAATGCAGCAGACGGTGTTCAGCATTCTCGGTATTAACGA
GCACGAACAGCGCGAGAAGTTTGGCTTCCTGCTGGACGCATTGAAGTACGGCACGCCGCCACACGCCGGCCTGGCTTTTG
GCCTGGATCGTCTGGTAATGCTGCTGACCGGTACCGACAACATTCGTGACGTCATCGCCTTCCCGAAAACCACGGCAGCG
GCCTGTCTGATGACCGAAGCGCCGAGCTTCGCCAACCCGGCGTCGCTGCAGGAGCTGGCGATCTGCGTGGTGAAAAAAGC
CTCTGAACAAGAGAGCGAGTGA

Upstream 100 bases:

>100_bases
AACCGGGTAATAAGTAGAGTTCATCAGAAATCATCAGAAAACAGCCGCGTGGCAGGCGCTAAAGCCCGCGCGGCATCGGC
TTAAGCCAAAAGGATAGCGT

Downstream 100 bases:

>100_bases
TGAATTACAAGCGACCCGAGTCTATTCTGGTAGTGATTTATGCAAAATCCAGTGGTCGGGTGCTGATGTTACAGCGGCGC
GATGATATCGAATTCTGGCA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MRTEYCGQLNLSHVGQEVTLCGWVNRRRDLGGLIFIDMRDREGIVQVFFDPDQKAAFDKAYDLRNEFCIQIVGTVRARPD
SQINKDMATGEVEVFAHALEIINRSEPLPLDSNQVNSEEARLKYRYLDLRRPEMADRLKTRAKITSFVRRFMDSHGFLDI
ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFMT
AEQVREVMEKLARELWLDVKNVDLGDFPIMTFEEAMRRYGSDKPDLRNPLELVDVADLVKDVEFKVFSGPANDAKGRVAA
ICVPGGAQLTRKLIDEYGAFVNIYGAKGLAWLKVNDRAAGMEGVQSPIAKFLSAEVLEAILARTNAQSGDILFFGADSFK
IVTDAMGALRLKLGRDLELTKLDSWAPLWVVDFPMFEEDGEGGLAAMHHPFTAPRDMSPEELAGTPTNAIANAYDMVING
YEVGGGSVRIHRSEMQQTVFSILGINEHEQREKFGFLLDALKYGTPPHAGLAFGLDRLVMLLTGTDNIRDVIAFPKTTAA
ACLMTEAPSFANPASLQELAICVVKKASEQESE

Sequences:

>Translated_593_residues
MRTEYCGQLNLSHVGQEVTLCGWVNRRRDLGGLIFIDMRDREGIVQVFFDPDQKAAFDKAYDLRNEFCIQIVGTVRARPD
SQINKDMATGEVEVFAHALEIINRSEPLPLDSNQVNSEEARLKYRYLDLRRPEMADRLKTRAKITSFVRRFMDSHGFLDI
ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFMT
AEQVREVMEKLARELWLDVKNVDLGDFPIMTFEEAMRRYGSDKPDLRNPLELVDVADLVKDVEFKVFSGPANDAKGRVAA
ICVPGGAQLTRKLIDEYGAFVNIYGAKGLAWLKVNDRAAGMEGVQSPIAKFLSAEVLEAILARTNAQSGDILFFGADSFK
IVTDAMGALRLKLGRDLELTKLDSWAPLWVVDFPMFEEDGEGGLAAMHHPFTAPRDMSPEELAGTPTNAIANAYDMVING
YEVGGGSVRIHRSEMQQTVFSILGINEHEQREKFGFLLDALKYGTPPHAGLAFGLDRLVMLLTGTDNIRDVIAFPKTTAA
ACLMTEAPSFANPASLQELAICVVKKASEQESE
>Mature_593_residues
MRTEYCGQLNLSHVGQEVTLCGWVNRRRDLGGLIFIDMRDREGIVQVFFDPDQKAAFDKAYDLRNEFCIQIVGTVRARPD
SQINKDMATGEVEVFAHALEIINRSEPLPLDSNQVNSEEARLKYRYLDLRRPEMADRLKTRAKITSFVRRFMDSHGFLDI
ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFMT
AEQVREVMEKLARELWLDVKNVDLGDFPIMTFEEAMRRYGSDKPDLRNPLELVDVADLVKDVEFKVFSGPANDAKGRVAA
ICVPGGAQLTRKLIDEYGAFVNIYGAKGLAWLKVNDRAAGMEGVQSPIAKFLSAEVLEAILARTNAQSGDILFFGADSFK
IVTDAMGALRLKLGRDLELTKLDSWAPLWVVDFPMFEEDGEGGLAAMHHPFTAPRDMSPEELAGTPTNAIANAYDMVING
YEVGGGSVRIHRSEMQQTVFSILGINEHEQREKFGFLLDALKYGTPPHAGLAFGLDRLVMLLTGTDNIRDVIAFPKTTAA
ACLMTEAPSFANPASLQELAICVVKKASEQESE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=598, Percent_Identity=40.6354515050167, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI5031815, Length=240, Percent_Identity=25.4166666666667, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI194272210, Length=240, Percent_Identity=25.4166666666667, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI45439306, Length=256, Percent_Identity=26.171875, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI1788173, Length=587, Percent_Identity=82.282793867121, Blast_Score=1017, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=312, Percent_Identity=28.2051282051282, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790571, Length=300, Percent_Identity=27, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI32566633, Length=614, Percent_Identity=34.5276872964169, Blast_Score=313, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI71994340, Length=154, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=154, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=154, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=273, Percent_Identity=27.4725274725275, Blast_Score=79, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71984122, Length=246, Percent_Identity=25.609756097561, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=637, Percent_Identity=35.3218210361067, Blast_Score=292, Evalue=9e-80,
Organism=Saccharomyces cerevisiae, GI6320242, Length=256, Percent_Identity=25.78125, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321807, Length=252, Percent_Identity=28.1746031746032, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6323011, Length=264, Percent_Identity=27.6515151515151, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24584738, Length=619, Percent_Identity=35.2180936995153, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24640849, Length=248, Percent_Identity=27.8225806451613, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24640851, Length=241, Percent_Identity=28.2157676348548, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI17136276, Length=239, Percent_Identity=27.1966527196653, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19921528, Length=231, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66484; Mature: 66484

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTEYCGQLNLSHVGQEVTLCGWVNRRRDLGGLIFIDMRDREGIVQVFFDPDQKAAFDKA
CCCCCCCCCCHHHCCCEEEEEHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHH
YDLRNEFCIQIVGTVRARPDSQINKDMATGEVEVFAHALEIINRSEPLPLDSNQVNSEEA
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
RLKYRYLDLRRPEMADRLKTRAKITSFVRRFMDSHGFLDIETPMLTKATPEGARDYLVPS
HEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCC
RVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFMT
CCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHHHH
AEQVREVMEKLARELWLDVKNVDLGDFPIMTFEEAMRRYGSDKPDLRNPLELVDVADLVK
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DVEFKVFSGPANDAKGRVAAICVPGGAQLTRKLIDEYGAFVNIYGAKGLAWLKVNDRAAG
HCCEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCC
MEGVQSPIAKFLSAEVLEAILARTNAQSGDILFFGADSFKIVTDAMGALRLKLGRDLELT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEE
KLDSWAPLWVVDFPMFEEDGEGGLAAMHHPFTAPRDMSPEELAGTPTNAIANAYDMVING
ECCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHEEC
YEVGGGSVRIHRSEMQQTVFSILGINEHEQREKFGFLLDALKYGTPPHAGLAFGLDRLVM
EEECCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEE
LLTGTDNIRDVIAFPKTTAAACLMTEAPSFANPASLQELAICVVKKASEQESE
EEECCCCHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRTEYCGQLNLSHVGQEVTLCGWVNRRRDLGGLIFIDMRDREGIVQVFFDPDQKAAFDKA
CCCCCCCCCCHHHCCCEEEEEHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHH
YDLRNEFCIQIVGTVRARPDSQINKDMATGEVEVFAHALEIINRSEPLPLDSNQVNSEEA
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
RLKYRYLDLRRPEMADRLKTRAKITSFVRRFMDSHGFLDIETPMLTKATPEGARDYLVPS
HEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCC
RVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFMT
CCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHHHH
AEQVREVMEKLARELWLDVKNVDLGDFPIMTFEEAMRRYGSDKPDLRNPLELVDVADLVK
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DVEFKVFSGPANDAKGRVAAICVPGGAQLTRKLIDEYGAFVNIYGAKGLAWLKVNDRAAG
HCCEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCC
MEGVQSPIAKFLSAEVLEAILARTNAQSGDILFFGADSFKIVTDAMGALRLKLGRDLELT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEE
KLDSWAPLWVVDFPMFEEDGEGGLAAMHHPFTAPRDMSPEELAGTPTNAIANAYDMVING
ECCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHEEC
YEVGGGSVRIHRSEMQQTVFSILGINEHEQREKFGFLLDALKYGTPPHAGLAFGLDRLVM
EEECCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEE
LLTGTDNIRDVIAFPKTTAAACLMTEAPSFANPASLQELAICVVKKASEQESE
EEECCCCHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA