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The map label for this gene is nudB [H]

Identifier: 157371020

GI number: 157371020

Start: 3060001

End: 3060447

Strand: Reverse

Name: nudB [H]

Synonym: Spro_2780

Alternate gene names: 157371020

Gene position: 3060447-3060001 (Counterclockwise)

Preceding gene: 157371021

Following gene: 157371019

Centisome position: 56.17

GC content: 53.24

Gene sequence:

>447_bases
ATGAATTACAAGCGACCCGAGTCTATTCTGGTAGTGATTTATGCAAAATCCAGTGGTCGGGTGCTGATGTTACAGCGGCG
CGATGATATCGAATTCTGGCAGTCGGTGACCGGCAGCCTGGAGGAAGATGAGTCGCCGCCGCATGCCGCCCGGCGTGAGG
TCATGGAAGAAGTCGGCATCGATATCGAAGCAGAGAATCTGCCGTTGTTCGATTGCCAGCGCTGTGTGGAGTTTGAACTC
TTTGTCCATTTGCGACATCGCTATGCCCCGGGAACCACGCGCAATAAAGAGCATTGGTTCTGTCTGGCTTTGCCTGAAGA
GCGCGATCCGGTGATTACCGAACATCACGCCTACCAGTGGCTTGAGGCGGTGCAGGCCGTGAAGTTGACCAAGTCCTGGA
GCAATCAGCAGGCGATTGAAGAGTTTGTGATTAATTCAGTCCAGTAA

Upstream 100 bases:

>100_bases
CCTGTCTGATGACCGAAGCGCCGAGCTTCGCCAACCCGGCGTCGCTGCAGGAGCTGGCGATCTGCGTGGTGAAAAAAGCC
TCTGAACAAGAGAGCGAGTG

Downstream 100 bases:

>100_bases
TTTTTTTCGGAGATTTTTTATGGCAGGTCATAGTAAGTGGGCCAACACCAAGCACCGCAAGGCAGCGCAGGATTCCAAGC
GCGGTAAAATTTTCACCAAA

Product: NUDIX hydrolase

Products: dAMP; pyrophosphate [C]

Alternate protein names: dATP pyrophosphohydrolase [H]

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MNYKRPESILVVIYAKSSGRVLMLQRRDDIEFWQSVTGSLEEDESPPHAARREVMEEVGIDIEAENLPLFDCQRCVEFEL
FVHLRHRYAPGTTRNKEHWFCLALPEERDPVITEHHAYQWLEAVQAVKLTKSWSNQQAIEEFVINSVQ

Sequences:

>Translated_148_residues
MNYKRPESILVVIYAKSSGRVLMLQRRDDIEFWQSVTGSLEEDESPPHAARREVMEEVGIDIEAENLPLFDCQRCVEFEL
FVHLRHRYAPGTTRNKEHWFCLALPEERDPVITEHHAYQWLEAVQAVKLTKSWSNQQAIEEFVINSVQ
>Mature_148_residues
MNYKRPESILVVIYAKSSGRVLMLQRRDDIEFWQSVTGSLEEDESPPHAARREVMEEVGIDIEAENLPLFDCQRCVEFEL
FVHLRHRYAPGTTRNKEHWFCLALPEERDPVITEHHAYQWLEAVQAVKLTKSWSNQQAIEEFVINSVQ

Specific function: Catalyzes the hydrolysis of dihydroneopterin triphosphate to dihydroneopterin monophosphate and pyrophosphate. Required for efficient folate biosynthesis. Can also hydrolyze nucleoside triphosphates with a preference for dATP [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788172, Length=144, Percent_Identity=72.2222222222222, Blast_Score=217, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003564
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 17383; Mature: 17383

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYKRPESILVVIYAKSSGRVLMLQRRDDIEFWQSVTGSLEEDESPPHAARREVMEEVGI
CCCCCCCEEEEEEEECCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCC
DIEAENLPLFDCQRCVEFELFVHLRHRYAPGTTRNKEHWFCLALPEERDPVITEHHAYQW
EEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHH
LEAVQAVKLTKSWSNQQAIEEFVINSVQ
HHHHHHHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNYKRPESILVVIYAKSSGRVLMLQRRDDIEFWQSVTGSLEEDESPPHAARREVMEEVGI
CCCCCCCEEEEEEEECCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCC
DIEAENLPLFDCQRCVEFELFVHLRHRYAPGTTRNKEHWFCLALPEERDPVITEHHAYQW
EEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHH
LEAVQAVKLTKSWSNQQAIEEFVINSVQ
HHHHHHHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxy-ATP; H2O [C]

Specific reaction: deoxy-ATP + H2O = dAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]