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The map label for this gene is topB [H]

Identifier: 157370958

GI number: 157370958

Start: 2993439

End: 2995364

Strand: Reverse

Name: topB [H]

Synonym: Spro_2718

Alternate gene names: 157370958

Gene position: 2995364-2993439 (Counterclockwise)

Preceding gene: 157370959

Following gene: 157370957

Centisome position: 54.97

GC content: 57.11

Gene sequence:

>1926_bases
ATGCGACTGTTTATTGCCGAGAAACCGAGTTTGGCGCGCGCCATTGCCGACGTACTTCCCAAGCCGCATCGCCGCGGCGA
CGGGTTTATCGCCTGCGGTAACAATGATGTGGTGACCTGGTGTGTGGGCCACTTGTTAGAACAGGCGCAGCCGGATGCCT
ACGACAGCCGTTATGCGCGCTGGTCGCTGGCCGATCTGCCGATCATCCCCGAAAAGTGGCAGTTGCAGCCACGGCCTTCG
GTCAGCAAACAGCTTAATGCCATCAAAAAGCTGCTGCACGAGGCCGATGAAGTGGTGCATGCCGGTGACCCGGACAGGGA
AGGCCAATTGCTGGTGGATGAGGTGCTGGACTATCTGGCGTTGTCGCCGGAAAAACGTCACAGCGTCCGCCGTTGCCTGA
TCAACGATCTCAACCCGCAGGCGGTGGAACGCGCTATCGAACGGCTGCGTGACAATCGCGACTTTATCCCACTTTGTGTC
TCCGCCCTGGCGCGTTCCCGCGCCGACTGGCTGTATGGCATCAACATGACCCGCGCCTACACCATTTTGGGGCGTAATGC
CGGTTATGATGGCGTGTTGTCGGTGGGCCGGGTGCAAACACCGGTGTTGGGGTTGGTCGTCAGGCGTGACGAAGATATTG
AAAATTTTGTCTCGAAGGACTTCTTCGAGGTTAAAGCGCACATCGTGACGCCTAAAGACGAACGTTTTGTCGCCCTGTGG
CAACCGAGTGACTCCTGCGAGCCTTATCAGGACGAAGAGGGGCGCTTGCTGCACCGATCGTTGGCAGAACACGTGCTCAA
GCGTATCGAGGGCCAGCCCGCGCTGGTCACCTCTTATAATGATAAACGGGAATCAGATACCGCACCCTTGCCGTTTTCAC
TGTCGACCTTACAGATTGAGGCTTCCAAACGCTTCAACCTGAGCGCGCAGCAGGTACTGGACGTGTGCCAGCGGCTGTAT
GAAACCCATAAATTGATCACCTATCCGCGTTCCGACTGCCGCTACCTGCCGGAAGAGCATTTTGCCGGGCGGCATTCGGT
GTTGAACGCCATCAGCGTTCATCAGCCGGATCTGTATCCGCAGCCGGTGATCGACAGCGATCGCCGTAACCGTTGCTGGG
ACGACAAGAAGGTTGATGCTCACCACGCCATTATTCCGACCGCCCGTGCCAGTAAGGTTAGCCTCAGCCAGGATGAGCTG
AACGTTTATGGCTTGGTGGCGCGGCAGTACCTGATGCAGTTCTGCCCGGACGCCATGTTCCGCAAATGCGTGATTGAGCT
GGATATTGCCGGCGGCAAGTTTATCGCCAAGGCGCGTTTTCTTGCCGAAGCAGGCTGGCGAACACTGTTGGGCAGTAAAG
AGCGTGATGAAGAAAATGAAGGCGCTCCGTTGCCGGTGGTGGCGAAGGATGACCAGCTACTGTGTGAACGTGGAGAGGTG
GTTGAACGTCAGACCCAACCGCCACGTCCTTTTACCGATGCCAGCCTGCTTTCCGCCATGACAGGGATTGCACGTTTCGT
TCAGGATAAAGCACTAAAGAAAATCCTGCGAGCGACCGATGGTTTAGGGACAGAGGCCACGCGCGCGGGCATCATTGAGT
TGCTGTTCAAGCGGGCGTTTTTGTATAAGAAAGGGCGCTATATTCACTCCAGTGAAACCGGCCGGGCGTTGATCCATTCG
CTGCCGGATATTGCCGCCCGTCCTGATATGACCGCCAACTGGGAAGCCACGCTGACGCAAATCAGTGAGAAATCCTGCCG
TTATCAGGACTTTATGCAACCTCTGGTAGGCACTCTGCAGGAGCTGATTTATCAGGCCAAGCAGAGTCGGGCGAGTATGG
CGTTTCGCGGGTTGCCGCCGTCGCCTTCCGGCGGGGCGAAAAAACGCAAGAAAAGCGCCGGTAAGGCGCGGGAGAAGAGC
GAATGA

Upstream 100 bases:

>100_bases
ACTATCAATTGGAAAAGAAATCCCAGCGGATTGTGTTCCGCGGGAATTATGCTGAAGTAAGAGAATTCCTGATGACGTAC
AGCCAGAATAACGGTGAATA

Downstream 100 bases:

>100_bases
ATCACGGCTTACTGTTGCTGGCCGGTAGCGCGCTGTTGTTTTGCAACGCGGCGATGGCCAATCGTGGCGATGTCGATGTG
GTGGTGCCAGTGTCGCCGGA

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MRLFIAEKPSLARAIADVLPKPHRRGDGFIACGNNDVVTWCVGHLLEQAQPDAYDSRYARWSLADLPIIPEKWQLQPRPS
VSKQLNAIKKLLHEADEVVHAGDPDREGQLLVDEVLDYLALSPEKRHSVRRCLINDLNPQAVERAIERLRDNRDFIPLCV
SALARSRADWLYGINMTRAYTILGRNAGYDGVLSVGRVQTPVLGLVVRRDEDIENFVSKDFFEVKAHIVTPKDERFVALW
QPSDSCEPYQDEEGRLLHRSLAEHVLKRIEGQPALVTSYNDKRESDTAPLPFSLSTLQIEASKRFNLSAQQVLDVCQRLY
ETHKLITYPRSDCRYLPEEHFAGRHSVLNAISVHQPDLYPQPVIDSDRRNRCWDDKKVDAHHAIIPTARASKVSLSQDEL
NVYGLVARQYLMQFCPDAMFRKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKDDQLLCERGEV
VERQTQPPRPFTDASLLSAMTGIARFVQDKALKKILRATDGLGTEATRAGIIELLFKRAFLYKKGRYIHSSETGRALIHS
LPDIAARPDMTANWEATLTQISEKSCRYQDFMQPLVGTLQELIYQAKQSRASMAFRGLPPSPSGGAKKRKKSAGKAREKS
E

Sequences:

>Translated_641_residues
MRLFIAEKPSLARAIADVLPKPHRRGDGFIACGNNDVVTWCVGHLLEQAQPDAYDSRYARWSLADLPIIPEKWQLQPRPS
VSKQLNAIKKLLHEADEVVHAGDPDREGQLLVDEVLDYLALSPEKRHSVRRCLINDLNPQAVERAIERLRDNRDFIPLCV
SALARSRADWLYGINMTRAYTILGRNAGYDGVLSVGRVQTPVLGLVVRRDEDIENFVSKDFFEVKAHIVTPKDERFVALW
QPSDSCEPYQDEEGRLLHRSLAEHVLKRIEGQPALVTSYNDKRESDTAPLPFSLSTLQIEASKRFNLSAQQVLDVCQRLY
ETHKLITYPRSDCRYLPEEHFAGRHSVLNAISVHQPDLYPQPVIDSDRRNRCWDDKKVDAHHAIIPTARASKVSLSQDEL
NVYGLVARQYLMQFCPDAMFRKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKDDQLLCERGEV
VERQTQPPRPFTDASLLSAMTGIARFVQDKALKKILRATDGLGTEATRAGIIELLFKRAFLYKKGRYIHSSETGRALIHS
LPDIAARPDMTANWEATLTQISEKSCRYQDFMQPLVGTLQELIYQAKQSRASMAFRGLPPSPSGGAKKRKKSAGKAREKS
E
>Mature_641_residues
MRLFIAEKPSLARAIADVLPKPHRRGDGFIACGNNDVVTWCVGHLLEQAQPDAYDSRYARWSLADLPIIPEKWQLQPRPS
VSKQLNAIKKLLHEADEVVHAGDPDREGQLLVDEVLDYLALSPEKRHSVRRCLINDLNPQAVERAIERLRDNRDFIPLCV
SALARSRADWLYGINMTRAYTILGRNAGYDGVLSVGRVQTPVLGLVVRRDEDIENFVSKDFFEVKAHIVTPKDERFVALW
QPSDSCEPYQDEEGRLLHRSLAEHVLKRIEGQPALVTSYNDKRESDTAPLPFSLSTLQIEASKRFNLSAQQVLDVCQRLY
ETHKLITYPRSDCRYLPEEHFAGRHSVLNAISVHQPDLYPQPVIDSDRRNRCWDDKKVDAHHAIIPTARASKVSLSQDEL
NVYGLVARQYLMQFCPDAMFRKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKDDQLLCERGEV
VERQTQPPRPFTDASLLSAMTGIARFVQDKALKKILRATDGLGTEATRAGIIELLFKRAFLYKKGRYIHSSETGRALIHS
LPDIAARPDMTANWEATLTQISEKSCRYQDFMQPLVGTLQELIYQAKQSRASMAFRGLPPSPSGGAKKRKKSAGKAREKS
E

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=645, Percent_Identity=22.7906976744186, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI10835218, Length=614, Percent_Identity=23.1270358306189, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1788061, Length=637, Percent_Identity=82.574568288854, Blast_Score=1118, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=629, Percent_Identity=23.3704292527822, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17555378, Length=660, Percent_Identity=23.7878787878788, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323263, Length=672, Percent_Identity=24.5535714285714, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24640096, Length=674, Percent_Identity=23.2937685459941, Blast_Score=116, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24585251, Length=478, Percent_Identity=26.9874476987448, Blast_Score=102, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 72376; Mature: 72376

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFIAEKPSLARAIADVLPKPHRRGDGFIACGNNDVVTWCVGHLLEQAQPDAYDSRYAR
CEEEECCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHCHHCE
WSLADLPIIPEKWQLQPRPSVSKQLNAIKKLLHEADEVVHAGDPDREGQLLVDEVLDYLA
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LSPEKRHSVRRCLINDLNPQAVERAIERLRDNRDFIPLCVSALARSRADWLYGINMTRAY
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEECCHHHEE
TILGRNAGYDGVLSVGRVQTPVLGLVVRRDEDIENFVSKDFFEVKAHIVTPKDERFVALW
EEEECCCCCCHHHHCCCHHCCHHHEEEECCCHHHHHHHHHHHHHHHEEECCCCCCEEEEE
QPSDSCEPYQDEEGRLLHRSLAEHVLKRIEGQPALVTSYNDKRESDTAPLPFSLSTLQIE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEEEEEE
ASKRFNLSAQQVLDVCQRLYETHKLITYPRSDCRYLPEEHFAGRHSVLNAISVHQPDLYP
CCCCCCCCHHHHHHHHHHHHHHHHEEECCCHHHCCCCHHHCCCHHHHHHHHHCCCCCCCC
QPVIDSDRRNRCWDDKKVDAHHAIIPTARASKVSLSQDELNVYGLVARQYLMQFCPDAMF
CCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHH
RKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKDDQLLCERGEV
HHHEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHHCCHH
VERQTQPPRPFTDASLLSAMTGIARFVQDKALKKILRATDGLGTEATRAGIIELLFKRAF
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LYKKGRYIHSSETGRALIHSLPDIAARPDMTANWEATLTQISEKSCRYQDFMQPLVGTLQ
HHHCCCEEECCCCHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELIYQAKQSRASMAFRGLPPSPSGGAKKRKKSAGKAREKSE
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRLFIAEKPSLARAIADVLPKPHRRGDGFIACGNNDVVTWCVGHLLEQAQPDAYDSRYAR
CEEEECCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHCHHCE
WSLADLPIIPEKWQLQPRPSVSKQLNAIKKLLHEADEVVHAGDPDREGQLLVDEVLDYLA
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LSPEKRHSVRRCLINDLNPQAVERAIERLRDNRDFIPLCVSALARSRADWLYGINMTRAY
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEECCHHHEE
TILGRNAGYDGVLSVGRVQTPVLGLVVRRDEDIENFVSKDFFEVKAHIVTPKDERFVALW
EEEECCCCCCHHHHCCCHHCCHHHEEEECCCHHHHHHHHHHHHHHHEEECCCCCCEEEEE
QPSDSCEPYQDEEGRLLHRSLAEHVLKRIEGQPALVTSYNDKRESDTAPLPFSLSTLQIE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEEEEEE
ASKRFNLSAQQVLDVCQRLYETHKLITYPRSDCRYLPEEHFAGRHSVLNAISVHQPDLYP
CCCCCCCCHHHHHHHHHHHHHHHHEEECCCHHHCCCCHHHCCCHHHHHHHHHCCCCCCCC
QPVIDSDRRNRCWDDKKVDAHHAIIPTARASKVSLSQDELNVYGLVARQYLMQFCPDAMF
CCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHH
RKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKDDQLLCERGEV
HHHEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHHCCHH
VERQTQPPRPFTDASLLSAMTGIARFVQDKALKKILRATDGLGTEATRAGIIELLFKRAF
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LYKKGRYIHSSETGRALIHSLPDIAARPDMTANWEATLTQISEKSCRYQDFMQPLVGTLQ
HHHCCCEEECCCCHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELIYQAKQSRASMAFRGLPPSPSGGAKKRKKSAGKAREKSE
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2553698; 9097039; 9278503; 10574789 [H]