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The map label for this gene is selU

Identifier: 157370959

GI number: 157370959

Start: 2995364

End: 2996482

Strand: Reverse

Name: selU

Synonym: Spro_2719

Alternate gene names: 157370959

Gene position: 2996482-2995364 (Counterclockwise)

Preceding gene: 157370960

Following gene: 157370958

Centisome position: 54.99

GC content: 56.75

Gene sequence:

>1119_bases
GTGTCAGCCACCAGGGAACGGGAAGACGGCCAGGATTATCGCCGTATTTTTTTGCAGGATATCCCGCTGATTGACGTGCG
TGCGCCGGTTGAGTTTCAGCAGGGGGCTTTCGCCAATGCTGTGAATCTGCCGTTAATGAACGACGACGAGCGGCAGGCCG
TCGGCACCTGTTACAAACAGCAAGGCCAGCAGGCAGCGATTGCGCTGGGCCACAGCCTGGTTAATGGCAGGCTTCGTGAG
CAACGTACGGCAGCCTGGCTGGCGCAATGTGCCCAATGGCCGGAAGGCTATATTTACTGTTTCCGCGGCGGTTTGCGTTC
TCAGTTGGTGCAGCAGTGGCTGCGTGAGGCCGGCGTCGCCTATCCGCGCATCACCGGCGGTTACAAGGCCCTGCGCAATT
TTTTGCTCACCACGCTGGAGCAGAGCGCTGAACTGCCGATGGTGCTGATCGGCGGCAATACCGGCAGCGGTAAAACTCTG
TTGGTTAATGAGCTGGCCGACGGCGTTGATCTGGAGGGGGCGGCGCACCACCGCGGTTCATCTTTTGGCCGAACGCTGGT
GAGTCAAAGCGGCCAGATTGATTTTGAGAATCGTCTGGCTGTGTTATTGCTGAAAAAACAGCATGGTGGCTGCCGCCGCT
GGGTGCTGGAAGACGAGGGGCGCATTATCGGCAGCAACAATCTGCCATTACCCGTCTTCAACCGCATGCAGCAGGCACCG
GTGGCGGTGATAGACGATCCTTTTGAGGTGCGTCTGGCACGGCTGCAGCACGAATATATCGACCGGATGCGTATTGAGTT
TGAACAGGCCTACGGCGCGCAACTTGGCTGGCAGAAGTACGATGAATATCTGCATCACGGCCTGTTTGCCATTCGCCGCC
GCCTGGGGCTGGAGCGTTTCCAACTGCTGACGCAGCACCTGGAACGGGCACTGCAGTATCAGCAGGCCAGTGGAAATAGT
GACGAGCATCAGCAGTGGCTGGTGCCGTTGTTGCAGCACTACTACGATCCGATGTATCACTATCAATTGGAAAAGAAATC
CCAGCGGATTGTGTTCCGCGGGAATTATGCTGAAGTAAGAGAATTCCTGATGACGTACAGCCAGAATAACGGTGAATAA

Upstream 100 bases:

>100_bases
AGCAGGTGCAGGCGATTGCCGAGCGCCACGGCATTCAACTCCAGGCTATCGGTGAACTGCATGCGGCAGTGCCGGGCAAG
CCGTTGATTGAGGTTGTCTA

Downstream 100 bases:

>100_bases
TGCGACTGTTTATTGCCGAGAAACCGAGTTTGGCGCGCGCCATTGCCGACGTACTTCCCAAGCCGCATCGCCGCGGCGAC
GGGTTTATCGCCTGCGGTAA

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSATREREDGQDYRRIFLQDIPLIDVRAPVEFQQGAFANAVNLPLMNDDERQAVGTCYKQQGQQAAIALGHSLVNGRLRE
QRTAAWLAQCAQWPEGYIYCFRGGLRSQLVQQWLREAGVAYPRITGGYKALRNFLLTTLEQSAELPMVLIGGNTGSGKTL
LVNELADGVDLEGAAHHRGSSFGRTLVSQSGQIDFENRLAVLLLKKQHGGCRRWVLEDEGRIIGSNNLPLPVFNRMQQAP
VAVIDDPFEVRLARLQHEYIDRMRIEFEQAYGAQLGWQKYDEYLHHGLFAIRRRLGLERFQLLTQHLERALQYQQASGNS
DEHQQWLVPLLQHYYDPMYHYQLEKKSQRIVFRGNYAEVREFLMTYSQNNGE

Sequences:

>Translated_372_residues
MSATREREDGQDYRRIFLQDIPLIDVRAPVEFQQGAFANAVNLPLMNDDERQAVGTCYKQQGQQAAIALGHSLVNGRLRE
QRTAAWLAQCAQWPEGYIYCFRGGLRSQLVQQWLREAGVAYPRITGGYKALRNFLLTTLEQSAELPMVLIGGNTGSGKTL
LVNELADGVDLEGAAHHRGSSFGRTLVSQSGQIDFENRLAVLLLKKQHGGCRRWVLEDEGRIIGSNNLPLPVFNRMQQAP
VAVIDDPFEVRLARLQHEYIDRMRIEFEQAYGAQLGWQKYDEYLHHGLFAIRRRLGLERFQLLTQHLERALQYQQASGNS
DEHQQWLVPLLQHYYDPMYHYQLEKKSQRIVFRGNYAEVREFLMTYSQNNGE
>Mature_371_residues
SATREREDGQDYRRIFLQDIPLIDVRAPVEFQQGAFANAVNLPLMNDDERQAVGTCYKQQGQQAAIALGHSLVNGRLREQ
RTAAWLAQCAQWPEGYIYCFRGGLRSQLVQQWLREAGVAYPRITGGYKALRNFLLTTLEQSAELPMVLIGGNTGSGKTLL
VNELADGVDLEGAAHHRGSSFGRTLVSQSGQIDFENRLAVLLLKKQHGGCRRWVLEDEGRIIGSNNLPLPVFNRMQQAPV
AVIDDPFEVRLARLQHEYIDRMRIEFEQAYGAQLGWQKYDEYLHHGLFAIRRRLGLERFQLLTQHLERALQYQQASGNSD
EHQQWLVPLLQHYYDPMYHYQLEKKSQRIVFRGNYAEVREFLMTYSQNNGE

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain

Homologues:

Organism=Escherichia coli, GI1786712, Length=360, Percent_Identity=57.7777777777778, Blast_Score=423, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SELU_SERP5 (A8GFD0)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001478948.1
- ProteinModelPortal:   A8GFD0
- SMR:   A8GFD0
- STRING:   A8GFD0
- GeneID:   5606741
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_2719
- eggNOG:   COG2603
- HOGENOM:   HBG452364
- OMA:   NLPLMND
- ProtClustDB:   PRK11784
- BioCyc:   SPRO399741:SPRO_2719-MONOMER
- HAMAP:   MF_01622
- InterPro:   IPR001763
- InterPro:   IPR017582
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00450
- TIGRFAMs:   TIGR03167

Pfam domain/function: PF00581 Rhodanese; SSF52821 Rhodanese-like

EC number: NA

Molecular weight: Translated: 42739; Mature: 42608

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS50206 RHODANESE_3

Important sites: ACT_SITE 100-100

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATREREDGQDYRRIFLQDIPLIDVRAPVEFQQGAFANAVNLPLMNDDERQAVGTCYKQ
CCCCCCCCCCHHHHHHHHHCCCEEEECCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
QGQQAAIALGHSLVNGRLREQRTAAWLAQCAQWPEGYIYCFRGGLRSQLVQQWLREAGVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCC
YPRITGGYKALRNFLLTTLEQSAELPMVLIGGNTGSGKTLLVNELADGVDLEGAAHHRGS
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEHHCCCCCCCCCCCCCCC
SFGRTLVSQSGQIDFENRLAVLLLKKQHGGCRRWVLEDEGRIIGSNNLPLPVFNRMQQAP
HHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCEEECCCCCCCHHHHHHHHCC
VAVIDDPFEVRLARLQHEYIDRMRIEFEQAYGAQLGWQKYDEYLHHGLFAIRRRLGLERF
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHH
QLLTQHLERALQYQQASGNSDEHQQWLVPLLQHYYDPMYHYQLEKKSQRIVFRGNYAEVR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCEEEEECCHHHHH
EFLMTYSQNNGE
HHHHHHHCCCCC
>Mature Secondary Structure 
SATREREDGQDYRRIFLQDIPLIDVRAPVEFQQGAFANAVNLPLMNDDERQAVGTCYKQ
CCCCCCCCCHHHHHHHHHCCCEEEECCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
QGQQAAIALGHSLVNGRLREQRTAAWLAQCAQWPEGYIYCFRGGLRSQLVQQWLREAGVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCC
YPRITGGYKALRNFLLTTLEQSAELPMVLIGGNTGSGKTLLVNELADGVDLEGAAHHRGS
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEHHCCCCCCCCCCCCCCC
SFGRTLVSQSGQIDFENRLAVLLLKKQHGGCRRWVLEDEGRIIGSNNLPLPVFNRMQQAP
HHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCEEECCCCCCCHHHHHHHHCC
VAVIDDPFEVRLARLQHEYIDRMRIEFEQAYGAQLGWQKYDEYLHHGLFAIRRRLGLERF
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHH
QLLTQHLERALQYQQASGNSDEHQQWLVPLLQHYYDPMYHYQLEKKSQRIVFRGNYAEVR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCEEEEECCHHHHH
EFLMTYSQNNGE
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA