Click here to switch to the map view.

The map label for this gene is hydN [H]

Identifier: 157370665

GI number: 157370665

Start: 2659849

End: 2660391

Strand: Reverse

Name: hydN [H]

Synonym: Spro_2425

Alternate gene names: 157370665

Gene position: 2660391-2659849 (Counterclockwise)

Preceding gene: 157370666

Following gene: 157370664

Centisome position: 48.82

GC content: 59.3

Gene sequence:

>543_bases
ATGAACCGGTTTATCATCGCCGATCCCAAGAAATGCATCGGCTGCCGTACCTGTGAAATTGCCTGCGTGATGGCACACAG
CGAAAACCAGGATATTTCAGCGCTCAACGCCGCCAGCTTCGCCCCACGGCTGCATGTGATCAAAGGCGTTAACGTCAGCA
CTGCCGTACTGTGCCGCCAGTGTGAGGACGCGCCTTGCTCCAACGTTTGCCCCAATGGGGCGATTAGCCGAACCAACGGC
ATGGTGCTGGTGATGCAGGAGCGCTGCATCGGCTGCAAAACCTGCGTGGTGGCCTGTCCTTACGGCGCGATGGAAGTGAT
CACCCGCCCGGTTGTCCGCCAGAACGGCGCCGTGATGAGTGCCACCACCGAGAAAGCCGAAGCCCATAAATGCGACCTGT
GCATCGGTCGTGATAGCGGCCCGGCCTGTATGCAAACCTGCCCGACCAATGCGTTGCACTGTGTGGATCGCAACATGCTG
CAAGAAATGAACGCCGAGAAACGCCGTCGCGCCGCGCTCGACACCCTGTCGTCACTGTTTTAG

Upstream 100 bases:

>100_bases
TGCTTTTATTTGATTTTCAGGGTTGGCACGCTTTATGCGTAACCCAAGGCACAACCTATCCATAGTCATACCCGGATGAA
GGGTAACTCAGGAGTTATTG

Downstream 100 bases:

>100_bases
GAACTGAACCGATGAAAAAAATCACAACCGTCTGCCCATACTGCGGTGCAGGCTGCAAAATGAAACTGGTGGTCGACAAC
GGAAAAATCATCCGCGCCGA

Product: electron transport protein HydN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MNRFIIADPKKCIGCRTCEIACVMAHSENQDISALNAASFAPRLHVIKGVNVSTAVLCRQCEDAPCSNVCPNGAISRTNG
MVLVMQERCIGCKTCVVACPYGAMEVITRPVVRQNGAVMSATTEKAEAHKCDLCIGRDSGPACMQTCPTNALHCVDRNML
QEMNAEKRRRAALDTLSSLF

Sequences:

>Translated_180_residues
MNRFIIADPKKCIGCRTCEIACVMAHSENQDISALNAASFAPRLHVIKGVNVSTAVLCRQCEDAPCSNVCPNGAISRTNG
MVLVMQERCIGCKTCVVACPYGAMEVITRPVVRQNGAVMSATTEKAEAHKCDLCIGRDSGPACMQTCPTNALHCVDRNML
QEMNAEKRRRAALDTLSSLF
>Mature_180_residues
MNRFIIADPKKCIGCRTCEIACVMAHSENQDISALNAASFAPRLHVIKGVNVSTAVLCRQCEDAPCSNVCPNGAISRTNG
MVLVMQERCIGCKTCVVACPYGAMEVITRPVVRQNGAVMSATTEKAEAHKCDLCIGRDSGPACMQTCPTNALHCVDRNML
QEMNAEKRRRAALDTLSSLF

Specific function: Electron transport from formate to hydrogen [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789067, Length=173, Percent_Identity=71.0982658959538, Blast_Score=262, Evalue=8e-72,
Organism=Escherichia coli, GI2367245, Length=161, Percent_Identity=55.2795031055901, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1788811, Length=172, Percent_Identity=49.4186046511628, Blast_Score=172, Evalue=1e-44,
Organism=Escherichia coli, GI87082179, Length=167, Percent_Identity=40.7185628742515, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI87082180, Length=179, Percent_Identity=37.9888268156425, Blast_Score=126, Evalue=8e-31,
Organism=Escherichia coli, GI1789079, Length=188, Percent_Identity=38.2978723404255, Blast_Score=120, Evalue=4e-29,
Organism=Escherichia coli, GI87082114, Length=194, Percent_Identity=34.5360824742268, Blast_Score=111, Evalue=2e-26,
Organism=Escherichia coli, GI1787872, Length=183, Percent_Identity=31.1475409836066, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787122, Length=183, Percent_Identity=31.6939890710383, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787749, Length=187, Percent_Identity=31.0160427807487, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI2367345, Length=189, Percent_Identity=29.6296296296296, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI226510944, Length=157, Percent_Identity=33.1210191082803, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1790326, Length=186, Percent_Identity=29.0322580645161, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787963, Length=170, Percent_Identity=27.0588235294118, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 19440; Mature: 19440

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
14.4 %Cys+Met (Translated Protein)
9.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
14.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFIIADPKKCIGCRTCEIACVMAHSENQDISALNAASFAPRLHVIKGVNVSTAVLCRQ
CCCEEECCCHHHHCCCCEEEEEEEECCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHH
CEDAPCSNVCPNGAISRTNGMVLVMQERCIGCKTCVVACPYGAMEVITRPVVRQNGAVMS
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHEEECCCCHHHHHHHHHHHCCCCEEE
ATTEKAEAHKCDLCIGRDSGPACMQTCPTNALHCVDRNMLQEMNAEKRRRAALDTLSSLF
ECCCCCCCCCCCEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRFIIADPKKCIGCRTCEIACVMAHSENQDISALNAASFAPRLHVIKGVNVSTAVLCRQ
CCCEEECCCHHHHCCCCEEEEEEEECCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHH
CEDAPCSNVCPNGAISRTNGMVLVMQERCIGCKTCVVACPYGAMEVITRPVVRQNGAVMS
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHEEECCCCHHHHHHHHHHHCCCCEEE
ATTEKAEAHKCDLCIGRDSGPACMQTCPTNALHCVDRNMLQEMNAEKRRRAALDTLSSLF
ECCCCCCCCCCCEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]