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The map label for this gene is hisJ [H]

Identifier: 157370637

GI number: 157370637

Start: 2618800

End: 2619573

Strand: Direct

Name: hisJ [H]

Synonym: Spro_2397

Alternate gene names: 157370637

Gene position: 2618800-2619573 (Clockwise)

Preceding gene: 157370636

Following gene: 157370638

Centisome position: 48.06

GC content: 54.26

Gene sequence:

>774_bases
ATGAAAAAAACATTAGCGGTTTTACTGACCAGTCTGGCATTAAGCGGCCCTGCGGCGGCTCAGACCTTCGACACCATCAG
TTTTGGCGTGGACGGCGGTTATCCGCCGTTTGACGTGCTGGCACCCAGCGGTGAGATCACCGGTTTCGACATCGATATCG
CCAATGCGCTGTGCACCCAGCTGCATGCCAAATGCGTGTTCGTCAAACAGCCGTTCGAAAGCATGATTGCCGCGCTTAAC
GCCCATAAATTCGATGCCATTATTGCGTCGTTAACCATTACCGATGAACGCAAGAAAGAGGTGGATTTTACCGACCGCTA
TTACCGCAGTTCTGCCCAGTTGGTGGCACGTAAGGGCAGCGCGTTACTGCCGGACGTTGCCAGCCTGAAAGGCAAAACCG
TTGGCGTGCAAACCGGCTCGATCCATGAGGCCTATGCCAAACAGCACTGGGGCGGGAAAGGGGTGAAAATTGTCTCTTAC
GCCAACCAGGATAACGTCTATCTGGATCTGATGTCCGGGCGCATTAACGCGTCACTGCAGGACAACACTCAGGCCGCCAG
CAGCTTTATCGATACCCCACGCGGGCAGAAGTTTGCCTTCGCCGGCCCGGTGATTGAAGACAGCAGTATCTCCTCTGACG
TTGGCATTGCGGTGGGTAAAGACAATCCGGCGCTGCGCGATGCGCTTAACGGGGCCATCAAGGCGATCCGTGCCGACGGG
ACTTATGAAGCCATCCAGAAAAAATATTTTAGCTTCGACATATATGGCAACTAA

Upstream 100 bases:

>100_bases
AATCGTTCAACTCGACACCAGCGAATTTAAAAAAATGGACGGCGGCCTGACCTGCCTGTCACTGCGTTTCTGACACTTTC
TTTTACCGGGGATCTCAGCC

Downstream 100 bases:

>100_bases
ACCGGCTCGGATCCAGTCATCCAGGGGAGGCCGGGAATGGTCATAGAGTATTTGCCGTTGCTGGCTCATGGCGCGTTGCT
GTCACTGTGCGTGATGCTGG

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKKTLAVLLTSLALSGPAAAQTFDTISFGVDGGYPPFDVLAPSGEITGFDIDIANALCTQLHAKCVFVKQPFESMIAALN
AHKFDAIIASLTITDERKKEVDFTDRYYRSSAQLVARKGSALLPDVASLKGKTVGVQTGSIHEAYAKQHWGGKGVKIVSY
ANQDNVYLDLMSGRINASLQDNTQAASSFIDTPRGQKFAFAGPVIEDSSISSDVGIAVGKDNPALRDALNGAIKAIRADG
TYEAIQKKYFSFDIYGN

Sequences:

>Translated_257_residues
MKKTLAVLLTSLALSGPAAAQTFDTISFGVDGGYPPFDVLAPSGEITGFDIDIANALCTQLHAKCVFVKQPFESMIAALN
AHKFDAIIASLTITDERKKEVDFTDRYYRSSAQLVARKGSALLPDVASLKGKTVGVQTGSIHEAYAKQHWGGKGVKIVSY
ANQDNVYLDLMSGRINASLQDNTQAASSFIDTPRGQKFAFAGPVIEDSSISSDVGIAVGKDNPALRDALNGAIKAIRADG
TYEAIQKKYFSFDIYGN
>Mature_257_residues
MKKTLAVLLTSLALSGPAAAQTFDTISFGVDGGYPPFDVLAPSGEITGFDIDIANALCTQLHAKCVFVKQPFESMIAALN
AHKFDAIIASLTITDERKKEVDFTDRYYRSSAQLVARKGSALLPDVASLKGKTVGVQTGSIHEAYAKQHWGGKGVKIVSY
ANQDNVYLDLMSGRINASLQDNTQAASSFIDTPRGQKFAFAGPVIEDSSISSDVGIAVGKDNPALRDALNGAIKAIRADG
TYEAIQKKYFSFDIYGN

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=44.6351931330472, Blast_Score=229, Evalue=1e-61,
Organism=Escherichia coli, GI1788649, Length=260, Percent_Identity=43.4615384615385, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1787085, Length=252, Percent_Identity=40.4761904761905, Blast_Score=174, Evalue=5e-45,
Organism=Escherichia coli, GI1787088, Length=253, Percent_Identity=37.5494071146245, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787031, Length=251, Percent_Identity=33.0677290836653, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1788228, Length=231, Percent_Identity=32.4675324675325, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27481; Mature: 27481

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTLAVLLTSLALSGPAAAQTFDTISFGVDGGYPPFDVLAPSGEITGFDIDIANALCTQ
CCHHHHHHHHHHHCCCCCHHHHHHHEEECCCCCCCCHHEECCCCCEEEEEEHHHHHHHHH
LHAKCVFVKQPFESMIAALNAHKFDAIIASLTITDERKKEVDFTDRYYRSSAQLVARKGS
HHHEEEEEECCHHHHHHHHCCHHHHEEEEEEEECCCHHHCCCHHHHHHHHHHHHHHHCCC
ALLPDVASLKGKTVGVQTGSIHEAYAKQHWGGKGVKIVSYANQDNVYLDLMSGRINASLQ
CCCCCHHHCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCC
DNTQAASSFIDTPRGQKFAFAGPVIEDSSISSDVGIAVGKDNPALRDALNGAIKAIRADG
CCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHEEECCC
TYEAIQKKYFSFDIYGN
HHHHHHHHHEEEEEECC
>Mature Secondary Structure
MKKTLAVLLTSLALSGPAAAQTFDTISFGVDGGYPPFDVLAPSGEITGFDIDIANALCTQ
CCHHHHHHHHHHHCCCCCHHHHHHHEEECCCCCCCCHHEECCCCCEEEEEEHHHHHHHHH
LHAKCVFVKQPFESMIAALNAHKFDAIIASLTITDERKKEVDFTDRYYRSSAQLVARKGS
HHHEEEEEECCHHHHHHHHCCHHHHEEEEEEEECCCHHHCCCHHHHHHHHHHHHHHHCCC
ALLPDVASLKGKTVGVQTGSIHEAYAKQHWGGKGVKIVSYANQDNVYLDLMSGRINASLQ
CCCCCHHHCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCC
DNTQAASSFIDTPRGQKFAFAGPVIEDSSISSDVGIAVGKDNPALRDALNGAIKAIRADG
CCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHEEECCC
TYEAIQKKYFSFDIYGN
HHHHHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]