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The map label for this gene is stp [H]

Identifier: 157370593

GI number: 157370593

Start: 2565279

End: 2566868

Strand: Reverse

Name: stp [H]

Synonym: Spro_2353

Alternate gene names: 157370593

Gene position: 2566868-2565279 (Counterclockwise)

Preceding gene: 157370595

Following gene: 157370592

Centisome position: 47.11

GC content: 55.79

Gene sequence:

>1590_bases
ATGCAAATCATTTATCCAGAATGGTTACTCGTTTCCGTAACCTTTCTTTTGTGGAAAAAATTCATGACGCTCTCTCACAC
CAGTAAAAACAGACTGGCATTAACGGCAATTTGCCTCGCCGCGCTGATGTTCGGCCTGGAAATCTCCAGTGTGCCGGTGA
TACTGCCGACGCTGGAACGGGTATTACACAGCGATTTCAGCGAAATGCAGTGGATTATGAATGCCTACACTCTGGCCTGC
ACCACAGTGATGATGGCCACCGGAACGCTGTCCGACCGTTTTGGCCGCAGGCGAGTTTTCATCATCGCCATTGTCGCTTT
TGGCCTCACTTCCTTACTCTGCGGCCTGGCCAACGGCTCGACGATGCTGATCGTGGGCCGCTTTATGCAAGGCATGGCGG
GCGGCGCCATGTTGATTTGCCAGGTAGCAGCATTGTCGCATCAGTTTGAGAACGGTAAACAGCGTGGCCAGGCCTTCGGC
ATTTGGGGCGTCGTTTTTGGCATTGGGTTAGGGTTTGGGCCGGCGATCGGCGGCGTTATCGTGGCGTTCGCCAACTGGCA
GTGGGTATTTTTGATTCATGTGGTCATCGCCGCTCTGACACTGTTACTGGTGATTTTCAGCGTGCAAGAATCCCGCGATC
CTCAGGCGGAAAAACTGGATAGTCTGGGGATCGTCACATTGTCGCTGTCTGTTTTTGGGTTGGTCTATTTTATCACTCAG
GGGGCGGGATTCGGTTTCACCAGCGTTCGCGCCGGGGGGATTCTTCTGGCTGCAGCCCTCAGCCTGCTGCTGTTTATCGG
GGTTGAGAGACGCAGCCCCTATCCGATGATTGAGTTTTCCGTGTTCAGGAATCGGCAATTTTCCGGGGCGCTGATGGGAT
CCATCGGCATGAATTTCAGCTTCTGGCCCTTTATGATCTACCTGCCGCTCTATTTTCAAATTGGGCTGGGTTATGACAGC
CTGACCACAGGTTTGTCGCTGCTCGCCTATACGCTTCCCACGCTTGTCTTTCCCCCGCTGGGTGAGCGATTGGCTCTGCG
TTTCCGCCCTGGAACCGTGATCCCCTCGGGGCTATTCGCGCTTGGATTGGGCTTCCTGCTGATGAAATCAGGTAGCAGCG
GCGAACATGCCGGCGGACTGGCTATGCTGCCGGGGTTACTTATCGCCGGCATCGGCCTGGGGATCACCAATACCCCGGTG
ACCAACACCACCACAGGTTCGGTGTCGAATGCCCGCGCCGGCATGGCCTCCGGGATAGACATGAGTGCCAGAATGATCGC
GTTGGCTGTCAATATTGCTGTCATGGGTTTTATCTTGCTGGCGGGCGTTGACGTTTACCTGCGACAAACGCTGCCCGAAT
CATTCGGTACCTTGCCGCTACGTTCGGTGGCGGAGCAGATAGTGGCGGGTAATCTCACCGCGTTGAAAACACTCCCCGAA
TGGGATGCCTTGGCCCCCTTGGTTCATGCTGCGTTGACGCAAAGTTTTGGCCTGGTGATGTTGTACGGCGGCCTCGGTGC
ATGGGCGTTGGCTGCGGCCAGCCTGATGATTTTCGTCCCCTGGAAAACTGCAGCCGCACTCCCCCGGTAA

Upstream 100 bases:

>100_bases
GGTAACCGCAACGAAGGTGCGCAGCAGTGTCGGGTCAATGTTCATAGGATGATTACATGATGGAATTTAATTAATAACAG
TAAATTAAATCATTTCTATC

Downstream 100 bases:

>100_bases
TAACATCCCGTTATTACCCAAAGGTTCATAAAATAAATAAAATATAATCGGGTGGTCTGAGACCGGCAGGGGCTTATGCT
GGCCCCAGACAACCTTGTCA

Product: major facilitator transporter

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MQIIYPEWLLVSVTFLLWKKFMTLSHTSKNRLALTAICLAALMFGLEISSVPVILPTLERVLHSDFSEMQWIMNAYTLAC
TTVMMATGTLSDRFGRRRVFIIAIVAFGLTSLLCGLANGSTMLIVGRFMQGMAGGAMLICQVAALSHQFENGKQRGQAFG
IWGVVFGIGLGFGPAIGGVIVAFANWQWVFLIHVVIAALTLLLVIFSVQESRDPQAEKLDSLGIVTLSLSVFGLVYFITQ
GAGFGFTSVRAGGILLAAALSLLLFIGVERRSPYPMIEFSVFRNRQFSGALMGSIGMNFSFWPFMIYLPLYFQIGLGYDS
LTTGLSLLAYTLPTLVFPPLGERLALRFRPGTVIPSGLFALGLGFLLMKSGSSGEHAGGLAMLPGLLIAGIGLGITNTPV
TNTTTGSVSNARAGMASGIDMSARMIALAVNIAVMGFILLAGVDVYLRQTLPESFGTLPLRSVAEQIVAGNLTALKTLPE
WDALAPLVHAALTQSFGLVMLYGGLGAWALAAASLMIFVPWKTAAALPR

Sequences:

>Translated_529_residues
MQIIYPEWLLVSVTFLLWKKFMTLSHTSKNRLALTAICLAALMFGLEISSVPVILPTLERVLHSDFSEMQWIMNAYTLAC
TTVMMATGTLSDRFGRRRVFIIAIVAFGLTSLLCGLANGSTMLIVGRFMQGMAGGAMLICQVAALSHQFENGKQRGQAFG
IWGVVFGIGLGFGPAIGGVIVAFANWQWVFLIHVVIAALTLLLVIFSVQESRDPQAEKLDSLGIVTLSLSVFGLVYFITQ
GAGFGFTSVRAGGILLAAALSLLLFIGVERRSPYPMIEFSVFRNRQFSGALMGSIGMNFSFWPFMIYLPLYFQIGLGYDS
LTTGLSLLAYTLPTLVFPPLGERLALRFRPGTVIPSGLFALGLGFLLMKSGSSGEHAGGLAMLPGLLIAGIGLGITNTPV
TNTTTGSVSNARAGMASGIDMSARMIALAVNIAVMGFILLAGVDVYLRQTLPESFGTLPLRSVAEQIVAGNLTALKTLPE
WDALAPLVHAALTQSFGLVMLYGGLGAWALAAASLMIFVPWKTAAALPR
>Mature_529_residues
MQIIYPEWLLVSVTFLLWKKFMTLSHTSKNRLALTAICLAALMFGLEISSVPVILPTLERVLHSDFSEMQWIMNAYTLAC
TTVMMATGTLSDRFGRRRVFIIAIVAFGLTSLLCGLANGSTMLIVGRFMQGMAGGAMLICQVAALSHQFENGKQRGQAFG
IWGVVFGIGLGFGPAIGGVIVAFANWQWVFLIHVVIAALTLLLVIFSVQESRDPQAEKLDSLGIVTLSLSVFGLVYFITQ
GAGFGFTSVRAGGILLAAALSLLLFIGVERRSPYPMIEFSVFRNRQFSGALMGSIGMNFSFWPFMIYLPLYFQIGLGYDS
LTTGLSLLAYTLPTLVFPPLGERLALRFRPGTVIPSGLFALGLGFLLMKSGSSGEHAGGLAMLPGLLIAGIGLGITNTPV
TNTTTGSVSNARAGMASGIDMSARMIALAVNIAVMGFILLAGVDVYLRQTLPESFGTLPLRSVAEQIVAGNLTALKTLPE
WDALAPLVHAALTQSFGLVMLYGGLGAWALAAASLMIFVPWKTAAALPR

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=394, Percent_Identity=29.1878172588832, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1790195, Length=324, Percent_Identity=30.2469135802469, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789042, Length=315, Percent_Identity=25.0793650793651, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788710, Length=320, Percent_Identity=27.1875, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1788392, Length=310, Percent_Identity=27.4193548387097, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790146, Length=193, Percent_Identity=25.9067357512953, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=292, Percent_Identity=28.4246575342466, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324954, Length=226, Percent_Identity=25.2212389380531, Blast_Score=73, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=193, Percent_Identity=27.4611398963731, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=196, Percent_Identity=26.530612244898, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56517; Mature: 56517

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHEEEEECCHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHEEEEECCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]