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The map label for this gene is estB [H]

Identifier: 157370515

GI number: 157370515

Start: 2476366

End: 2477517

Strand: Direct

Name: estB [H]

Synonym: Spro_2275

Alternate gene names: 157370515

Gene position: 2476366-2477517 (Clockwise)

Preceding gene: 157370513

Following gene: 157370518

Centisome position: 45.45

GC content: 60.07

Gene sequence:

>1152_bases
ATGTCGGAACTGAATCAGTCATTAATAAAAAGAGTACAGGCGGTTAGCCAGAAAGCGATAGCCGAACAGCGCATCGTCGG
CAGCGTGGTGCTGGTGGCGCAGCACGGCAAGGTCATTTACGCCGAGGCTAGCGGTTACGCCGACCGAGAACAACAGAAGT
TGATGCGTCGTGAAACACAGTTCCGACTGTCGTCGGTGTCCAAGCCCTATATCACACTGGCGGCGATGCGCATGGTCGAA
CAGCACAAGCTGGGGCTGGATGACGCGGTCAGCCGCTGGCTGCCCTGGTTCACTCCGTCGTTGGCCGATGGCCGTCAGCC
AGAAATTAAAATCCGCCACTTGCTCAGCCACAGCGCCGGACTGGGCTATCGTTTTAATCAGCCCGTTGATGGGCCGTATC
AGCGGTTGGGTATTCAAGATGGGCTGGCGTTGTCGTCGCTGACGCTGGAGCAGAATTTACGTCTGTTGGCGCAGGCAGAT
CTGTTGGCGGAACCCGGCAGCGAATTTCATTATTCTCTGGCGATCGACGTGCTGGGAGCGGTGCTGGAGCAGGTGGCGGG
CAAGCCGTTGCCGCAGGTGTTCGAACACTGGGTCGCGCAGCCGTTGGGATTGGGCAATACCGGTTTCTACACCAGTGACG
CCGACAACCTGGCGACCGCGTATCACGATACCGCCACCAGGCCGGAACCTTTACTGGATGGCATGCTGCTGACACTGCCG
GAGGAGTTCGGCTTTGATATCGAAATGTCGCCGTCACGAGCGCTTGATCCGTCGGCTTTTGCCTCTGGCGGGGCGGGTAT
GGTCGGTGATGCTGACGACGTGCTGCGCATGGTGGAGACGTTACGCAGCGGCGGTGAGGGCATTTTACAGCCGGCCAGCG
TGGAGCTGATGCGCCAGCCGCATGTCGGGGCCGAGGCTGAGGTTCAGGGGCCGGGCTGGGGCTTTGGTTTTGGTGGGGCG
GTGCTGGTGGATGCCCAGTTGGCGGCGACGCCACAGCACAACGGCACCCTGCAGTGGGGTGGCGTTTATGGACACAGTTG
GTTTTACGATCCGCAGGCGGCATTGAGCGTGGTGGCGCTGACCAACACGGCGTTTGAAGGCATGAGCGGGATGTATCCTC
AGCAGATACGCGATGCGGTGTACGGCGATTAA

Upstream 100 bases:

>100_bases
ATCAGTAATTCCTGCTTAATGATTCGTTAAGTAATTGGGTGTTTAAATTTATAATAATCGTTTTAGACTGGTTTTATTAT
CCCTTAGAGAGCAACAAAAC

Downstream 100 bases:

>100_bases
GGATAAAACAGGGGCCCGAGCAAGCGGGCCCCGAGGGGAAATATTACTGGGCGGGTGCCGGAGCAGGGACTGCGTCCGGA
GCCGGAGTTTCGACCGGGCC

Product: beta-lactamase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MSELNQSLIKRVQAVSQKAIAEQRIVGSVVLVAQHGKVIYAEASGYADREQQKLMRRETQFRLSSVSKPYITLAAMRMVE
QHKLGLDDAVSRWLPWFTPSLADGRQPEIKIRHLLSHSAGLGYRFNQPVDGPYQRLGIQDGLALSSLTLEQNLRLLAQAD
LLAEPGSEFHYSLAIDVLGAVLEQVAGKPLPQVFEHWVAQPLGLGNTGFYTSDADNLATAYHDTATRPEPLLDGMLLTLP
EEFGFDIEMSPSRALDPSAFASGGAGMVGDADDVLRMVETLRSGGEGILQPASVELMRQPHVGAEAEVQGPGWGFGFGGA
VLVDAQLAATPQHNGTLQWGGVYGHSWFYDPQAALSVVALTNTAFEGMSGMYPQQIRDAVYGD

Sequences:

>Translated_383_residues
MSELNQSLIKRVQAVSQKAIAEQRIVGSVVLVAQHGKVIYAEASGYADREQQKLMRRETQFRLSSVSKPYITLAAMRMVE
QHKLGLDDAVSRWLPWFTPSLADGRQPEIKIRHLLSHSAGLGYRFNQPVDGPYQRLGIQDGLALSSLTLEQNLRLLAQAD
LLAEPGSEFHYSLAIDVLGAVLEQVAGKPLPQVFEHWVAQPLGLGNTGFYTSDADNLATAYHDTATRPEPLLDGMLLTLP
EEFGFDIEMSPSRALDPSAFASGGAGMVGDADDVLRMVETLRSGGEGILQPASVELMRQPHVGAEAEVQGPGWGFGFGGA
VLVDAQLAATPQHNGTLQWGGVYGHSWFYDPQAALSVVALTNTAFEGMSGMYPQQIRDAVYGD
>Mature_382_residues
SELNQSLIKRVQAVSQKAIAEQRIVGSVVLVAQHGKVIYAEASGYADREQQKLMRRETQFRLSSVSKPYITLAAMRMVEQ
HKLGLDDAVSRWLPWFTPSLADGRQPEIKIRHLLSHSAGLGYRFNQPVDGPYQRLGIQDGLALSSLTLEQNLRLLAQADL
LAEPGSEFHYSLAIDVLGAVLEQVAGKPLPQVFEHWVAQPLGLGNTGFYTSDADNLATAYHDTATRPEPLLDGMLLTLPE
EFGFDIEMSPSRALDPSAFASGGAGMVGDADDVLRMVETLRSGGEGILQPASVELMRQPHVGAEAEVQGPGWGFGFGGAV
LVDAQLAATPQHNGTLQWGGVYGHSWFYDPQAALSVVALTNTAFEGMSGMYPQQIRDAVYGD

Specific function: Acts on short-chain (C4-C6) fatty acid esters and triglycerides, including tertiary alcohol esters. Activity on p- nitrophenyl esters is generally higher than on o-nitrophenyl esters. Lacks beta-lactamase activity; it hydrolyzes the ester bond of cephalos

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-A beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI87082101, Length=370, Percent_Identity=26.7567567567568, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115533026, Length=384, Percent_Identity=25.5208333333333, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR000871 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: NA

Molecular weight: Translated: 41515; Mature: 41384

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELNQSLIKRVQAVSQKAIAEQRIVGSVVLVAQHGKVIYAEASGYADREQQKLMRRETQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEECCCCCCHHHHHHHHHHHH
FRLSSVSKPYITLAAMRMVEQHKLGLDDAVSRWLPWFTPSLADGRQPEIKIRHLLSHSAG
HHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCC
LGYRFNQPVDGPYQRLGIQDGLALSSLTLEQNLRLLAQADLLAEPGSEFHYSLAIDVLGA
CCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VLEQVAGKPLPQVFEHWVAQPLGLGNTGFYTSDADNLATAYHDTATRPEPLLDGMLLTLP
HHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHCCHHEECH
EEFGFDIEMSPSRALDPSAFASGGAGMVGDADDVLRMVETLRSGGEGILQPASVELMRQP
HHHCCCEEECCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCC
HVGAEAEVQGPGWGFGFGGAVLVDAQLAATPQHNGTLQWGGVYGHSWFYDPQAALSVVAL
CCCCCCEECCCCCCCCCCCEEEEECHHHCCCCCCCEEEECCEECCCCEECHHHHHEEHHH
TNTAFEGMSGMYPQQIRDAVYGD
HHHHHHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
SELNQSLIKRVQAVSQKAIAEQRIVGSVVLVAQHGKVIYAEASGYADREQQKLMRRETQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEECCCCCCHHHHHHHHHHHH
FRLSSVSKPYITLAAMRMVEQHKLGLDDAVSRWLPWFTPSLADGRQPEIKIRHLLSHSAG
HHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCC
LGYRFNQPVDGPYQRLGIQDGLALSSLTLEQNLRLLAQADLLAEPGSEFHYSLAIDVLGA
CCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VLEQVAGKPLPQVFEHWVAQPLGLGNTGFYTSDADNLATAYHDTATRPEPLLDGMLLTLP
HHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHCCHHEECH
EEFGFDIEMSPSRALDPSAFASGGAGMVGDADDVLRMVETLRSGGEGILQPASVELMRQP
HHHCCCEEECCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCC
HVGAEAEVQGPGWGFGFGGAVLVDAQLAATPQHNGTLQWGGVYGHSWFYDPQAALSVVAL
CCCCCCEECCCCCCCCCCCEEEEECHHHCCCCCCCEEEECCEECCCCEECHHHHHEEHHH
TNTAFEGMSGMYPQQIRDAVYGD
HHHHHHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11472796; 11847270 [H]