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The map label for this gene is malI [H]

Identifier: 157370511

GI number: 157370511

Start: 2472046

End: 2473104

Strand: Direct

Name: malI [H]

Synonym: Spro_2271

Alternate gene names: 157370511

Gene position: 2472046-2473104 (Clockwise)

Preceding gene: 157370507

Following gene: 157370513

Centisome position: 45.37

GC content: 61.95

Gene sequence:

>1059_bases
ATGCCCAGGATCGGGCTTGCAGAGGTCATGACCATCAAAAAGATCACCATCACCGATGTTGCACAGCAGGCCGGCGTCTC
GGTCACTACCGTTTCGCTGGTGCTGAGCGGCAAAGGGCGTATTTCACCCACCACCGTGGCGCGGGTGAACCAGGCGATTG
AGCGCTTGGGCTACGTGCGCAACCGTCAGGCGGCGACGCTACGTGGTGGCGAGTCGGGGGTGATTGGGCTGATCCTGCGT
GATATCTGCGAGCCGTTTTATGCAGAAATGACCGCGGGCCTGAGCGAGGTGCTGGAGGCGCACGGCAAAGTGCTGTTTCT
GACTCAAAGCGGTCGCGACGGCAAGGGGCTGATGCGCTGTTTTGATACTTTGCTCGAGCACGGCATTGACGGCATGGTGC
TGGCGGGCGGCGTACGCTCGGCTGAGGGCCTGAAGGAAAAGGCTGCGGAGCAGGGCGTGCCGCTGGTGTGCGCAGCGCGC
TCTAATGGGCTGGAAGGGGTCGACGTGGTGCGGCCGGACAACATGCAGGCGGCTAAAATGGCCACCGAATTTCTGATCAA
ACGCGGTCACAGCCAAATTGCCTATCTGGGTGGGCAAAGCAGTTCGTTAACCCGCGCAGAACGGTTGGGGGGCTTTTGTG
CCACGCTGGTGCAATATGGCCTGCCATTCCGCTCCGACTGGATTGTCGAATGTGACTGCCATCAACGGGCCGCGGCGGAG
GCGGCGGAAAACCTGCTACGGCAACATCCCAATATCAGCGCGCTGGTTTGCCACAAGGCGTCGGTGGCATTGGGCGCCTA
TTTTGGCATTGTGCGTAGTGGGCGCAGCATCGGTTCTGAGGGGGTGGATACCTATTACGGCCAGCAGGTGGCGCTGATTG
GTTTTGGCGACGTGCCTGAGGCCGAACTGACCGAACCGCCGCTGACGCTGGTCTCCAGTTCCGCGCGGGAAGTGGGGCGC
AGCGCCGCCTCACGCTTGCTGCAACGGATTGCCGACGCCGACTTACCGCCGCAAAACGTGATCCTGCCGCCATTGCTGAT
CAAGCGCGGCTCGGCCTGA

Upstream 100 bases:

>100_bases
ATCACAATTCCAGCGTGCACTTTACGGCGACAATGGCAAGACCAGTAAAACGTTTTACTGACCAAACAGATTCGGTATAC
CATTTGCCTTACCAGGCAGA

Downstream 100 bases:

>100_bases
CCTCATTGTTGTTCATCCGTGACCTGTTGCTGGATCCAACTGACAAACACTTTCATTGCCGGGGTCATATCGCGTGATTT
CAGGTGGGTCAGCCAGTAGC

Product: DNA-binding transcriptional repressor MalI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MPRIGLAEVMTIKKITITDVAQQAGVSVTTVSLVLSGKGRISPTTVARVNQAIERLGYVRNRQAATLRGGESGVIGLILR
DICEPFYAEMTAGLSEVLEAHGKVLFLTQSGRDGKGLMRCFDTLLEHGIDGMVLAGGVRSAEGLKEKAAEQGVPLVCAAR
SNGLEGVDVVRPDNMQAAKMATEFLIKRGHSQIAYLGGQSSSLTRAERLGGFCATLVQYGLPFRSDWIVECDCHQRAAAE
AAENLLRQHPNISALVCHKASVALGAYFGIVRSGRSIGSEGVDTYYGQQVALIGFGDVPEAELTEPPLTLVSSSAREVGR
SAASRLLQRIADADLPPQNVILPPLLIKRGSA

Sequences:

>Translated_352_residues
MPRIGLAEVMTIKKITITDVAQQAGVSVTTVSLVLSGKGRISPTTVARVNQAIERLGYVRNRQAATLRGGESGVIGLILR
DICEPFYAEMTAGLSEVLEAHGKVLFLTQSGRDGKGLMRCFDTLLEHGIDGMVLAGGVRSAEGLKEKAAEQGVPLVCAAR
SNGLEGVDVVRPDNMQAAKMATEFLIKRGHSQIAYLGGQSSSLTRAERLGGFCATLVQYGLPFRSDWIVECDCHQRAAAE
AAENLLRQHPNISALVCHKASVALGAYFGIVRSGRSIGSEGVDTYYGQQVALIGFGDVPEAELTEPPLTLVSSSAREVGR
SAASRLLQRIADADLPPQNVILPPLLIKRGSA
>Mature_351_residues
PRIGLAEVMTIKKITITDVAQQAGVSVTTVSLVLSGKGRISPTTVARVNQAIERLGYVRNRQAATLRGGESGVIGLILRD
ICEPFYAEMTAGLSEVLEAHGKVLFLTQSGRDGKGLMRCFDTLLEHGIDGMVLAGGVRSAEGLKEKAAEQGVPLVCAARS
NGLEGVDVVRPDNMQAAKMATEFLIKRGHSQIAYLGGQSSSLTRAERLGGFCATLVQYGLPFRSDWIVECDCHQRAAAEA
AENLLRQHPNISALVCHKASVALGAYFGIVRSGRSIGSEGVDTYYGQQVALIGFGDVPEAELTEPPLTLVSSSAREVGRS
AASRLLQRIADADLPPQNVILPPLLIKRGSA

Specific function: Repressor for the malX and malY genes. Also regulates its own expression. Binds maltose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787906, Length=337, Percent_Identity=55.786350148368, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1790194, Length=337, Percent_Identity=27.0029673590504, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1790369, Length=316, Percent_Identity=27.2151898734177, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789068, Length=338, Percent_Identity=28.1065088757396, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI48994940, Length=334, Percent_Identity=27.5449101796407, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788474, Length=346, Percent_Identity=25.7225433526012, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787948, Length=337, Percent_Identity=25.8160237388724, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789202, Length=336, Percent_Identity=25.8928571428571, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786540, Length=341, Percent_Identity=24.633431085044, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787580, Length=333, Percent_Identity=25.2252252252252, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790715, Length=332, Percent_Identity=24.6987951807229, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786268, Length=308, Percent_Identity=24.6753246753247, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790689, Length=353, Percent_Identity=25.7790368271955, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37377; Mature: 37245

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS50943 HTH_CROC1 ; PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRIGLAEVMTIKKITITDVAQQAGVSVTTVSLVLSGKGRISPTTVARVNQAIERLGYVR
CCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
NRQAATLRGGESGVIGLILRDICEPFYAEMTAGLSEVLEAHGKVLFLTQSGRDGKGLMRC
CCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
FDTLLEHGIDGMVLAGGVRSAEGLKEKAAEQGVPLVCAARSNGLEGVDVVRPDNMQAAKM
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCHHHHH
ATEFLIKRGHSQIAYLGGQSSSLTRAERLGGFCATLVQYGLPFRSDWIVECDCHQRAAAE
HHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHH
AAENLLRQHPNISALVCHKASVALGAYFGIVRSGRSIGSEGVDTYYGQQVALIGFGDVPE
HHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEECCCCCC
AELTEPPLTLVSSSAREVGRSAASRLLQRIADADLPPQNVILPPLLIKRGSA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCC
>Mature Secondary Structure 
PRIGLAEVMTIKKITITDVAQQAGVSVTTVSLVLSGKGRISPTTVARVNQAIERLGYVR
CCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
NRQAATLRGGESGVIGLILRDICEPFYAEMTAGLSEVLEAHGKVLFLTQSGRDGKGLMRC
CCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
FDTLLEHGIDGMVLAGGVRSAEGLKEKAAEQGVPLVCAARSNGLEGVDVVRPDNMQAAKM
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCHHHHH
ATEFLIKRGHSQIAYLGGQSSSLTRAERLGGFCATLVQYGLPFRSDWIVECDCHQRAAAE
HHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHH
AAENLLRQHPNISALVCHKASVALGAYFGIVRSGRSIGSEGVDTYYGQQVALIGFGDVPE
HHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEECCCCCC
AELTEPPLTLVSSSAREVGRSAASRLLQRIADADLPPQNVILPPLLIKRGSA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670898; 1856179; 9097039; 9278503 [H]