Click here to switch to the map view.

The map label for this gene is tyrS

Identifier: 157370459

GI number: 157370459

Start: 2419629

End: 2420903

Strand: Direct

Name: tyrS

Synonym: Spro_2219

Alternate gene names: 157370459

Gene position: 2419629-2420903 (Clockwise)

Preceding gene: 157370458

Following gene: 157370460

Centisome position: 44.41

GC content: 56.47

Gene sequence:

>1275_bases
ATGGCAAGCAGCAACTTGATTAAACAACTGCAAGAGCGGGGCCTCGTTGCCCAGGTTACGGATGAAGAAGCGTTAGCAGA
GCGACTGGCGCAAGGGCCAATTGCACTGTATTGCGGTTTCGATCCGACCGCTGACAGCTTGCATTTGGGCCATCTGGTTC
CTCTGTTGTGCCTGAAGCGCTTCCAGCTCGCAGGCCACAAGCCGGTAGCCTTGGTTGGCGGTGCCACCGGCCTTATCGGT
GACCCGAGCTTCAAGGCGGCTGAGCGCAAGCTCAATACCACCGAAACGGTGAATGAGTGGGTGGATAAAATCCGCAAACA
GGTTTCTCCGTTCCTCGATTTCGACTGTGGTAGCAACAGCGCAATTGCCGCCAACAACTACGACTGGTTTGGCGGCATGA
ACGTGCTGACCTTCCTGCGTGACATCGGCAAGCATTTTTCCGTCAACCAGATGATCAACAAGGAAGCGGTGAAGCAGCGT
CTGAACCGTGACGACTCGGGCATTTCTTTCACCGAGTTCTCCTATAACCTGTTGCAGGGCTTCGACTTCTCCGAGCTGTA
CAACCGTCATCAGGTTGAACTGCAGATCGGCGGTTCCGACCAGTGGGGCAATATCACCTCCGGTATCGACCTGACCCGTC
GCATGCACCAGCAACAGGTATTCGGTCTGACCGTGCCCTTGATCACCAAGGCTGACGGCACCAAATTCGGCAAGACCGAA
GGTGGCGCAGTCTGGCTGGCACCAGAAAAAACCAGCCCGTACAAGTTCTACCAGTTCTGGATCAATACCGCCGATGCCGA
CGTGTACCGCTTCCTGAAGTTCTTCACCTTTATGGATCTGGCCGAGATCAACGCGCTGGAAGAAGAAGACAAAAACAGCG
GTAAAGCGCCACGCGCCCAGTATGTTCTGGCAGAAGAAGTGACCGGTATGGTACACGGTGCGGAAGGTCTGGCTGCGGCC
AAGCGTATCACCCAGAGCCTGTTCTCTGGCGCGCTGCACGAGATGACCGAAGCAGACTTCGCCCAGTTGGCACAGGACGG
CATGCCGACCATCAAGCTCGATGGCGACGCCGATCTGCAGCAGGCGTTGGTTAACGCTGAGCTGGTGCCTTCACGCGGCC
AGGCGCGCACCATGATCGGTTCCAATGCGGTGACCATCAACGGTGAGAAGCAGTCCAACGCCGAATACAACTTCAGCGAT
GCCGATCGTCTGTTCGGTCGCTACACGCTGCTGCGCCGCGGCAAAAAACATTACTGCCTGGTTGATTGGCAGTAA

Upstream 100 bases:

>100_bases
CGGCGCTTTATTCTATGCGTTATTCTGAATTTAATATTTCGCCAGTGGGCGAAAGGTCGTGTACCGACAAAGGTGCATTC
GTTTATACATGGAGTCATTG

Downstream 100 bases:

>100_bases
ACAAAGTTGCAGCAAAGGGGCCCTCAGCGGCCCCTTTATTTTTAAGGCCTCAGAGCAGGTTTTTGATGAAAAACATTCTT
TCTATTCAATCCCATGTGGT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKRFQLAGHKPVALVGGATGLIG
DPSFKAAERKLNTTETVNEWVDKIRKQVSPFLDFDCGSNSAIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEAVKQR
LNRDDSGISFTEFSYNLLQGFDFSELYNRHQVELQIGGSDQWGNITSGIDLTRRMHQQQVFGLTVPLITKADGTKFGKTE
GGAVWLAPEKTSPYKFYQFWINTADADVYRFLKFFTFMDLAEINALEEEDKNSGKAPRAQYVLAEEVTGMVHGAEGLAAA
KRITQSLFSGALHEMTEADFAQLAQDGMPTIKLDGDADLQQALVNAELVPSRGQARTMIGSNAVTINGEKQSNAEYNFSD
ADRLFGRYTLLRRGKKHYCLVDWQ

Sequences:

>Translated_424_residues
MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKRFQLAGHKPVALVGGATGLIG
DPSFKAAERKLNTTETVNEWVDKIRKQVSPFLDFDCGSNSAIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEAVKQR
LNRDDSGISFTEFSYNLLQGFDFSELYNRHQVELQIGGSDQWGNITSGIDLTRRMHQQQVFGLTVPLITKADGTKFGKTE
GGAVWLAPEKTSPYKFYQFWINTADADVYRFLKFFTFMDLAEINALEEEDKNSGKAPRAQYVLAEEVTGMVHGAEGLAAA
KRITQSLFSGALHEMTEADFAQLAQDGMPTIKLDGDADLQQALVNAELVPSRGQARTMIGSNAVTINGEKQSNAEYNFSD
ADRLFGRYTLLRRGKKHYCLVDWQ
>Mature_423_residues
ASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKRFQLAGHKPVALVGGATGLIGD
PSFKAAERKLNTTETVNEWVDKIRKQVSPFLDFDCGSNSAIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEAVKQRL
NRDDSGISFTEFSYNLLQGFDFSELYNRHQVELQIGGSDQWGNITSGIDLTRRMHQQQVFGLTVPLITKADGTKFGKTEG
GAVWLAPEKTSPYKFYQFWINTADADVYRFLKFFTFMDLAEINALEEEDKNSGKAPRAQYVLAEEVTGMVHGAEGLAAAK
RITQSLFSGALHEMTEADFAQLAQDGMPTIKLDGDADLQQALVNAELVPSRGQARTMIGSNAVTINGEKQSNAEYNFSDA
DRLFGRYTLLRRGKKHYCLVDWQ

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=406, Percent_Identity=36.9458128078818, Blast_Score=246, Evalue=3e-65,
Organism=Escherichia coli, GI1787925, Length=424, Percent_Identity=84.9056603773585, Blast_Score=755, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25145802, Length=408, Percent_Identity=35.2941176470588, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI25145805, Length=331, Percent_Identity=32.9305135951662, Blast_Score=155, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6325160, Length=439, Percent_Identity=35.9908883826879, Blast_Score=268, Evalue=2e-72,
Organism=Drosophila melanogaster, GI20130383, Length=445, Percent_Identity=35.7303370786517, Blast_Score=283, Evalue=2e-76,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_SERP5 (A8GDY0)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001478448.1
- ProteinModelPortal:   A8GDY0
- SMR:   A8GDY0
- STRING:   A8GDY0
- GeneID:   5605266
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_2219
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   TFYIGFD
- ProtClustDB:   PRK05912
- BioCyc:   SPRO399741:SPRO_2219-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 47069; Mature: 46937

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 37-37 BINDING 175-175 BINDING 179-179 BINDING 238-238

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKR
CCCHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
FQLAGHKPVALVGGATGLIGDPSFKAAERKLNTTETVNEWVDKIRKQVSPFLDFDCGSNS
HHHCCCCCEEEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCEECCCCCCC
AIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEAVKQRLNRDDSGISFTEFSYNLLQG
EEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHC
FDFSELYNRHQVELQIGGSDQWGNITSGIDLTRRMHQQQVFGLTVPLITKADGTKFGKTE
CCHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCC
GGAVWLAPEKTSPYKFYQFWINTADADVYRFLKFFTFMDLAEINALEEEDKNSGKAPRAQ
CCEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
YVLAEEVTGMVHGAEGLAAAKRITQSLFSGALHEMTEADFAQLAQDGMPTIKLDGDADLQ
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHH
QALVNAELVPSRGQARTMIGSNAVTINGEKQSNAEYNFSDADRLFGRYTLLRRGKKHYCL
HHHHCCCCCCCCCCCEEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEE
VDWQ
EECC
>Mature Secondary Structure 
ASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKR
CCHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
FQLAGHKPVALVGGATGLIGDPSFKAAERKLNTTETVNEWVDKIRKQVSPFLDFDCGSNS
HHHCCCCCEEEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCEECCCCCCC
AIAANNYDWFGGMNVLTFLRDIGKHFSVNQMINKEAVKQRLNRDDSGISFTEFSYNLLQG
EEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHC
FDFSELYNRHQVELQIGGSDQWGNITSGIDLTRRMHQQQVFGLTVPLITKADGTKFGKTE
CCHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCC
GGAVWLAPEKTSPYKFYQFWINTADADVYRFLKFFTFMDLAEINALEEEDKNSGKAPRAQ
CCEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
YVLAEEVTGMVHGAEGLAAAKRITQSLFSGALHEMTEADFAQLAQDGMPTIKLDGDADLQ
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHH
QALVNAELVPSRGQARTMIGSNAVTINGEKQSNAEYNFSDADRLFGRYTLLRRGKKHYCL
HHHHCCCCCCCCCCCEEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEE
VDWQ
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA