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The map label for this gene is nemA [H]

Identifier: 157370442

GI number: 157370442

Start: 2403264

End: 2404361

Strand: Reverse

Name: nemA [H]

Synonym: Spro_2202

Alternate gene names: 157370442

Gene position: 2404361-2403264 (Counterclockwise)

Preceding gene: 157370443

Following gene: 157370440

Centisome position: 44.13

GC content: 57.19

Gene sequence:

>1098_bases
ATGAAGATTGAAAAATTGTTCTCCCCGCTGAAGGTTGGCGCTGTAACCCTGCCGAACCGCGTGTTTATGGCCCCGCTGAC
GCGCCTGCGCAGCATTGAGCCTGGTGATATCCCGACGCCGCTGATGGGAGAATATTATGCCCAACGCACCAGCGCGGGTC
TGATTGTCACCGAAGCCACCCAGATTTCTTTCCAGGGGAAAGGCTATGCCGGCGCACCGGGGCTGCATACCCCAGAGCAG
ATCGCCGCGTGGAAAAAGATCACTCAGGCGGTGCATGACAAAAACGGCCATATCGCCGTTCAGCTGTGGCACGTAGGCCG
TATTTCCCATAACAGCCTGCAGCCAGGCCAACAGGCTCCGGTTGCCCCTTCTGCGATCAATGCCGACACCCGTACTACCG
TACGTGACGAAACCGGGGCCTGGGTACGAGTACCAACCTCTACCCCACGCGCCCTGGAAACTGAAGAGATCCCAGGCATC
ATTAACGATTTCCGCCAGGCGACTGCCAACGCCCGTGAGGCCGGTTTCGACTTTATCGAACTGCATGCGGCGCACGGCTA
TCTGCTGCACCAGTTTATGGCTCCGGCATCTAACCAACGTACCGATCAGTACGGCGGCAGCATCGAAAACCGCACTCGCC
TGACACTGGAAGTGGTTGACGCCAGCATCGCTGAATGGGGTTCAGAACACGTCGGTATCCGTATTTCACCGCTGGGCCCG
TTCAACGGCCTGGATAATGGTGAAGATCAGGAAGACGCGGCGCTGTATCTGATTGAAGAGCTGAACAAGCGTAACATCGC
TTTCCTGCACATTTCCGAACCCGACTGGGCCGGTGGCAAGCCTTACTCCGATGCCTTCCGTGACTCGGTACGCGCCCACT
TTAAAGGGGTGATTGTCGGTGCCGGTGCCTATACCGCCGAGAAAGCAGAAGAGCTGATCAATAAAGGCTTTATCGACGCC
GTGGCCTTTGGCCGTAGTTATATCGCCAACCCGGACCTGGTTGAGCGCTTCAAGCAGAGCGCGGAGCTGAACGAACCTAA
ACCGGAAACTTTCTACGGTGGTGGTGCTGAAGGCTACACCGACTACCCAACGCTGTAA

Upstream 100 bases:

>100_bases
CCGGCGCAGCGCTAAAAAAAACCTGATTTATCATGAACTACCGCATTGGGCGGTTTTACATTCGTTACTAGTCGACCAGT
CTATTAATATAGGATGCACT

Downstream 100 bases:

>100_bases
GTAAAACAGCAAAGCCCGCCGGTATGCCAATGCCGGCGGGCTTTTTGCCATTTAGGGGGAAACCTGTCAGTTAACTCGCA
GCCAACCTTGCCCAACGTTC

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKIEKLFSPLKVGAVTLPNRVFMAPLTRLRSIEPGDIPTPLMGEYYAQRTSAGLIVTEATQISFQGKGYAGAPGLHTPEQ
IAAWKKITQAVHDKNGHIAVQLWHVGRISHNSLQPGQQAPVAPSAINADTRTTVRDETGAWVRVPTSTPRALETEEIPGI
INDFRQATANAREAGFDFIELHAAHGYLLHQFMAPASNQRTDQYGGSIENRTRLTLEVVDASIAEWGSEHVGIRISPLGP
FNGLDNGEDQEDAALYLIEELNKRNIAFLHISEPDWAGGKPYSDAFRDSVRAHFKGVIVGAGAYTAEKAEELINKGFIDA
VAFGRSYIANPDLVERFKQSAELNEPKPETFYGGGAEGYTDYPTL

Sequences:

>Translated_365_residues
MKIEKLFSPLKVGAVTLPNRVFMAPLTRLRSIEPGDIPTPLMGEYYAQRTSAGLIVTEATQISFQGKGYAGAPGLHTPEQ
IAAWKKITQAVHDKNGHIAVQLWHVGRISHNSLQPGQQAPVAPSAINADTRTTVRDETGAWVRVPTSTPRALETEEIPGI
INDFRQATANAREAGFDFIELHAAHGYLLHQFMAPASNQRTDQYGGSIENRTRLTLEVVDASIAEWGSEHVGIRISPLGP
FNGLDNGEDQEDAALYLIEELNKRNIAFLHISEPDWAGGKPYSDAFRDSVRAHFKGVIVGAGAYTAEKAEELINKGFIDA
VAFGRSYIANPDLVERFKQSAELNEPKPETFYGGGAEGYTDYPTL
>Mature_365_residues
MKIEKLFSPLKVGAVTLPNRVFMAPLTRLRSIEPGDIPTPLMGEYYAQRTSAGLIVTEATQISFQGKGYAGAPGLHTPEQ
IAAWKKITQAVHDKNGHIAVQLWHVGRISHNSLQPGQQAPVAPSAINADTRTTVRDETGAWVRVPTSTPRALETEEIPGI
INDFRQATANAREAGFDFIELHAAHGYLLHQFMAPASNQRTDQYGGSIENRTRLTLEVVDASIAEWGSEHVGIRISPLGP
FNGLDNGEDQEDAALYLIEELNKRNIAFLHISEPDWAGGKPYSDAFRDSVRAHFKGVIVGAGAYTAEKAEELINKGFIDA
VAFGRSYIANPDLVERFKQSAELNEPKPETFYGGGAEGYTDYPTL

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=74.2465753424657, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI1789463, Length=242, Percent_Identity=35.5371900826446, Blast_Score=121, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17565138, Length=351, Percent_Identity=28.7749287749288, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17559802, Length=203, Percent_Identity=35.9605911330049, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=263, Percent_Identity=30.4182509505703, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=263, Percent_Identity=32.319391634981, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17559804, Length=269, Percent_Identity=28.6245353159851, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=269, Percent_Identity=27.8810408921933, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17566914, Length=309, Percent_Identity=24.5954692556634, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6325086, Length=387, Percent_Identity=38.7596899224806, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321973, Length=384, Percent_Identity=37.7604166666667, Blast_Score=223, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39921; Mature: 39921

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIEKLFSPLKVGAVTLPNRVFMAPLTRLRSIEPGDIPTPLMGEYYAQRTSAGLIVTEAT
CCHHHHHCCHHCCEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEEE
QISFQGKGYAGAPGLHTPEQIAAWKKITQAVHDKNGHIAVQLWHVGRISHNSLQPGQQAP
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCC
VAPSAINADTRTTVRDETGAWVRVPTSTPRALETEEIPGIINDFRQATANAREAGFDFIE
CCCCCCCCCCCCEEECCCCCEEECCCCCCCCCCHHHCCHHHHHHHHHHCCHHHHCCCEEE
LHAAHGYLLHQFMAPASNQRTDQYGGSIENRTRLTLEVVDASIAEWGSEHVGIRISPLGP
EEHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEEEEHHHHHHHCCCCCCCEEEECCCC
FNGLDNGEDQEDAALYLIEELNKRNIAFLHISEPDWAGGKPYSDAFRDSVRAHFKGVIVG
CCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCEEEEE
AGAYTAEKAEELINKGFIDAVAFGRSYIANPDLVERFKQSAELNEPKPETFYGGGAEGYT
CCCCCHHHHHHHHHCCCHHHHHHCHHHCCCCHHHHHHHHHHCCCCCCCCCEECCCCCCCC
DYPTL
CCCCC
>Mature Secondary Structure
MKIEKLFSPLKVGAVTLPNRVFMAPLTRLRSIEPGDIPTPLMGEYYAQRTSAGLIVTEAT
CCHHHHHCCHHCCEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEEE
QISFQGKGYAGAPGLHTPEQIAAWKKITQAVHDKNGHIAVQLWHVGRISHNSLQPGQQAP
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCC
VAPSAINADTRTTVRDETGAWVRVPTSTPRALETEEIPGIINDFRQATANAREAGFDFIE
CCCCCCCCCCCCEEECCCCCEEECCCCCCCCCCHHHCCHHHHHHHHHHCCHHHHCCCEEE
LHAAHGYLLHQFMAPASNQRTDQYGGSIENRTRLTLEVVDASIAEWGSEHVGIRISPLGP
EEHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEEEEHHHHHHHCCCCCCCEEEECCCC
FNGLDNGEDQEDAALYLIEELNKRNIAFLHISEPDWAGGKPYSDAFRDSVRAHFKGVIVG
CCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCEEEEE
AGAYTAEKAEELINKGFIDAVAFGRSYIANPDLVERFKQSAELNEPKPETFYGGGAEGYT
CCCCCHHHHHHHHHCCCHHHHHHCHHHCCCCHHHHHHHHHHCCCCCCCCCEECCCCCCCC
DYPTL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]