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The map label for this gene is gloA [H]

Identifier: 157370440

GI number: 157370440

Start: 2401915

End: 2402322

Strand: Reverse

Name: gloA [H]

Synonym: Spro_2200

Alternate gene names: 157370440

Gene position: 2402322-2401915 (Counterclockwise)

Preceding gene: 157370442

Following gene: 157370439

Centisome position: 44.09

GC content: 55.88

Gene sequence:

>408_bases
ATGCGCTTACTCCATACCATGATCCGCGTCGGTGACCTGCAACGCTCCATCGACTTCTATACCAAGGTATTAGGCATGCG
CTTGCTGCGTACCAGCGAGAACCCGGAATACAAGTACTCACTGGCGTTTGTAGGCTATACCGATGAAAGCGAAGGTGCGG
TGATTGAACTGACCTATAACTGGGGCACCGACAGCTACGAAATGGGCACCGCATTCGGCCACCTGGCACTGGGTGTGGAT
GATGTCGCCGCAACCTGTGACAGCATTCGTAATGCCGGCGGCAAGGTCACTCGCGAAGCCGGCCCGGTCAAGGGCGGCAC
CACGGTTATCGCCTTTGTTGAAGATCCGGACGGTTATAAAATCGAACTGATCGAAAACAAACACGCCGGCCACGGCCTCG
GCCACTAA

Upstream 100 bases:

>100_bases
CCTGCCGTGGCATACATTGAAATCCCTTATGCGGCAACGCTATACTGGATTTGCTTCAATCCATCCGCCATTGGCAACGG
CACATCCAATCAGAGGAATT

Downstream 100 bases:

>100_bases
GGTCAACCAGGGGCGCTTACTGCGCCCTTGTTTTTCCCGCGTGATTACCGCCTGCAACCGGGCGCAAAATTTGCCATAAT
GCGCGCTGCATTCGATGAAG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MRLLHTMIRVGDLQRSIDFYTKVLGMRLLRTSENPEYKYSLAFVGYTDESEGAVIELTYNWGTDSYEMGTAFGHLALGVD
DVAATCDSIRNAGGKVTREAGPVKGGTTVIAFVEDPDGYKIELIENKHAGHGLGH

Sequences:

>Translated_135_residues
MRLLHTMIRVGDLQRSIDFYTKVLGMRLLRTSENPEYKYSLAFVGYTDESEGAVIELTYNWGTDSYEMGTAFGHLALGVD
DVAATCDSIRNAGGKVTREAGPVKGGTTVIAFVEDPDGYKIELIENKHAGHGLGH
>Mature_135_residues
MRLLHTMIRVGDLQRSIDFYTKVLGMRLLRTSENPEYKYSLAFVGYTDESEGAVIELTYNWGTDSYEMGTAFGHLALGVD
DVAATCDSIRNAGGKVTREAGPVKGGTTVIAFVEDPDGYKIELIENKHAGHGLGH

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=152, Percent_Identity=38.1578947368421, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1787940, Length=135, Percent_Identity=81.4814814814815, Blast_Score=231, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6323639, Length=148, Percent_Identity=40.5405405405405, Blast_Score=100, Evalue=7e-23,
Organism=Drosophila melanogaster, GI20129743, Length=149, Percent_Identity=37.5838926174497, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19920404, Length=139, Percent_Identity=32.3741007194245, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14771; Mature: 14771

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLHTMIRVGDLQRSIDFYTKVLGMRLLRTSENPEYKYSLAFVGYTDESEGAVIELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCEEEEEEEC
WGTDSYEMGTAFGHLALGVDDVAATCDSIRNAGGKVTREAGPVKGGTTVIAFVEDPDGYK
CCCCCEECCCHHHHEEECHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECCCCCE
IELIENKHAGHGLGH
EEEEECCCCCCCCCC
>Mature Secondary Structure
MRLLHTMIRVGDLQRSIDFYTKVLGMRLLRTSENPEYKYSLAFVGYTDESEGAVIELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCEEEEEEEC
WGTDSYEMGTAFGHLALGVDDVAATCDSIRNAGGKVTREAGPVKGGTTVIAFVEDPDGYK
CCCCCEECCCHHHHEEECHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECCCCCE
IELIENKHAGHGLGH
EEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]