Click here to switch to the map view.

The map label for this gene is rnt [H]

Identifier: 157370439

GI number: 157370439

Start: 2401156

End: 2401836

Strand: Reverse

Name: rnt [H]

Synonym: Spro_2199

Alternate gene names: 157370439

Gene position: 2401836-2401156 (Counterclockwise)

Preceding gene: 157370440

Following gene: 157370437

Centisome position: 44.08

GC content: 53.3

Gene sequence:

>681_bases
ATGCGCGCTGCATTCGATGAAGATAAGAAACTGATGGCCGAAAAAAGTGATCTTAACGCCCTGAGTGGCCGTTTTCGTGG
GTTTTACCCCGTAGTAATAGATATAGAAACTGCCGGATTTAATGCCAAAACTGACGCGCTGCTGGAGATTGCCGCCGTTA
CGTTAAAAATGGATGAAGACGGCTGGTTGCAGCAGGACGAAACCCTGCATTTTCACGTGGAGCCTTTTGAAGGTGCCAAC
CTGCAACCCGAAGCGCTGGCTTTTAACGGCATAGATCCGAGTAATCCCCTGCGCGGTGCGGTCAGTGAATACGATGCACT
GCACGCTATTTTTAAAGCGGTGCGCAAGGGCATTAAGGATCGCGGCTGTAACCGGGCGATAATTGTGGCGCATAACGCCA
ATTTTGATCACAGTTTCCTGATGGCTGCCGCCGAGCGTGCCAGCCTGAAACGCAATCCTTTCCATCCTTTCGCCACCTTT
GACACCGCGGCGTTAAGCGGACTGGTGTTGGGACAAACGGTACTGGCCAAAGCCTGCATTGCCGCCGATATTCCCTTCGA
TAGTGCCCAGGCCCATTCAGCGCTGTATGATACCGAGCAAACTGCTCAGCTGTTCTGCGAGCTGGTTAACCGCTGGAAAC
GCCTTGGCGGTTGGCCGCTGGCGGTCACGAATACCGACTAA

Upstream 100 bases:

>100_bases
CGGCCACGGCCTCGGCCACTAAGGTCAACCAGGGGCGCTTACTGCGCCCTTGTTTTTCCCGCGTGATTACCGCCTGCAAC
CGGGCGCAAAATTTGCCATA

Downstream 100 bases:

>100_bases
GAAAAAAAATCGGGCGGCCCAGTATTAGCTGGCACCGCCCGATGACTTCCAACAATAAAACCGAGCCGATTATGGCTGCT
GGTCTTCCTGCTGTTTGTAC

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MRAAFDEDKKLMAEKSDLNALSGRFRGFYPVVIDIETAGFNAKTDALLEIAAVTLKMDEDGWLQQDETLHFHVEPFEGAN
LQPEALAFNGIDPSNPLRGAVSEYDALHAIFKAVRKGIKDRGCNRAIIVAHNANFDHSFLMAAAERASLKRNPFHPFATF
DTAALSGLVLGQTVLAKACIAADIPFDSAQAHSALYDTEQTAQLFCELVNRWKRLGGWPLAVTNTD

Sequences:

>Translated_226_residues
MRAAFDEDKKLMAEKSDLNALSGRFRGFYPVVIDIETAGFNAKTDALLEIAAVTLKMDEDGWLQQDETLHFHVEPFEGAN
LQPEALAFNGIDPSNPLRGAVSEYDALHAIFKAVRKGIKDRGCNRAIIVAHNANFDHSFLMAAAERASLKRNPFHPFATF
DTAALSGLVLGQTVLAKACIAADIPFDSAQAHSALYDTEQTAQLFCELVNRWKRLGGWPLAVTNTD
>Mature_226_residues
MRAAFDEDKKLMAEKSDLNALSGRFRGFYPVVIDIETAGFNAKTDALLEIAAVTLKMDEDGWLQQDETLHFHVEPFEGAN
LQPEALAFNGIDPSNPLRGAVSEYDALHAIFKAVRKGIKDRGCNRAIIVAHNANFDHSFLMAAAERASLKRNPFHPFATF
DTAALSGLVLGQTVLAKACIAADIPFDSAQAHSALYDTEQTAQLFCELVNRWKRLGGWPLAVTNTD

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=210, Percent_Identity=81.4285714285714, Blast_Score=363, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 24807; Mature: 24807

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAAFDEDKKLMAEKSDLNALSGRFRGFYPVVIDIETAGFNAKTDALLEIAAVTLKMDED
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHEEEEEEEEECCC
GWLQQDETLHFHVEPFEGANLQPEALAFNGIDPSNPLRGAVSEYDALHAIFKAVRKGIKD
CCCCCCCEEEEEECCCCCCCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RGCNRAIIVAHNANFDHSFLMAAAERASLKRNPFHPFATFDTAALSGLVLGQTVLAKACI
CCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AADIPFDSAQAHSALYDTEQTAQLFCELVNRWKRLGGWPLAVTNTD
HHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCC
>Mature Secondary Structure
MRAAFDEDKKLMAEKSDLNALSGRFRGFYPVVIDIETAGFNAKTDALLEIAAVTLKMDED
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHEEEEEEEEECCC
GWLQQDETLHFHVEPFEGANLQPEALAFNGIDPSNPLRGAVSEYDALHAIFKAVRKGIKD
CCCCCCCEEEEEECCCCCCCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RGCNRAIIVAHNANFDHSFLMAAAERASLKRNPFHPFATFDTAALSGLVLGQTVLAKACI
CCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AADIPFDSAQAHSALYDTEQTAQLFCELVNRWKRLGGWPLAVTNTD
HHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA