Click here to switch to the map view.

The map label for this gene is btuC

Identifier: 157370391

GI number: 157370391

Start: 2347370

End: 2348377

Strand: Direct

Name: btuC

Synonym: Spro_2151

Alternate gene names: 157370391

Gene position: 2347370-2348377 (Clockwise)

Preceding gene: 157370390

Following gene: 157370392

Centisome position: 43.08

GC content: 60.22

Gene sequence:

>1008_bases
ATGCCAGCCAGCCAGACCTTTACCTTATTGCTGCACAAACAGCGCCATCGCGATAAACGCCATCTGTTGTTGTTAACGCT
GGGCGTTGCCGTGGCCTTTGTTTTCAGCCTCAGTGCGGGCGACGTTTGGCTTTGGCCCTCACAGTGGTCGGGTGAGGCCG
CGCAGCTGTTTGTCTGGCAACTACGCCTGCCGCGCGCGCTGGCAGTGATGTTGGTAGGGGCCAGCCTGGCGGTGGCCGGG
GCGGTAATGCAGTCGCTGTTTGAAAATCCGCTGGCTGAGCCGGGTTTGCTGGGCGTCGCCAACGGCGCGGGGGTGGCACT
GGTGCTAACGGTGCTGCTGGGCAACGGCCTGCTGCCGGTAGCATTTATGAGCCTGAGCGCCATTGCCGGCGCGCTGGTCA
TGACCTTCCTGCTGCTGAGCTTTGCCCGTCGCAAACGTCTGACCAATGCGCGTCTACTGCTGGTGGGTGTTGCCTTGGGC
ATTGTGTGTAGCGCGGTGATGACCTGGGCGGTGTATTTTAGTTCCAGCCTGGACCTGCGCCAACTGATGTATTGGATGAT
GGGCGGTTTTGGCGGTGTAGACTGGCGGCAGAAATGGCTGGTGCTGGCATTGTTACCGGTGCTGCTTTGGCTGTGTTGCC
AGGGCAAGGCGCTAAACTTTATGGCGCTGGGTGAAGTACAGGCGCGTCAGCTCGGGCTTTCCCTGCATCTGTGGCGTAAT
CTACTGGTGCTGGCCATCGGCTGGCTGGTGGGCGTCAGTGTGGCTTTGGCCGGGGTGATTGGTTTTGTCGGACTGGTCAT
ACCGCACATGCTGCGTCTCAGTGGCCTGACCAATCAGCGATATTTATTGCCGGGCTGCGCACTGGCTGGCGCCGGTGTGT
TGCTGGCGGCCGATGTGGTGGCGCGTATTACGCTACTGTCGGCTGAATTACCCATCGGCGTGGTAACCGCCACGCTGGGG
GCACCGCTATTTATCTGGCTACTGACCCGGGTAAAAAGCTTAAGGTAG

Upstream 100 bases:

>100_bases
CCTGATGCAATGCTTTCCCTTCGGGCACTAACCCCCTAATATTTGACCCAGACGTCAATTATTCGGCCGCCTTGGCGGCC
AGCAGGGAATCGCCACGCCT

Downstream 100 bases:

>100_bases
TTTCTCTGTTCACCTGCAAGTAAAACAAGAGGATAAGCTCCATGACTCAAGCGATTTATGACATTCCGTTGAACACTATC
GAGAATCAATCGACCACGCT

Product: vtamin B12-transporter permease

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MPASQTFTLLLHKQRHRDKRHLLLLTLGVAVAFVFSLSAGDVWLWPSQWSGEAAQLFVWQLRLPRALAVMLVGASLAVAG
AVMQSLFENPLAEPGLLGVANGAGVALVLTVLLGNGLLPVAFMSLSAIAGALVMTFLLLSFARRKRLTNARLLLVGVALG
IVCSAVMTWAVYFSSSLDLRQLMYWMMGGFGGVDWRQKWLVLALLPVLLWLCCQGKALNFMALGEVQARQLGLSLHLWRN
LLVLAIGWLVGVSVALAGVIGFVGLVIPHMLRLSGLTNQRYLLPGCALAGAGVLLAADVVARITLLSAELPIGVVTATLG
APLFIWLLTRVKSLR

Sequences:

>Translated_335_residues
MPASQTFTLLLHKQRHRDKRHLLLLTLGVAVAFVFSLSAGDVWLWPSQWSGEAAQLFVWQLRLPRALAVMLVGASLAVAG
AVMQSLFENPLAEPGLLGVANGAGVALVLTVLLGNGLLPVAFMSLSAIAGALVMTFLLLSFARRKRLTNARLLLVGVALG
IVCSAVMTWAVYFSSSLDLRQLMYWMMGGFGGVDWRQKWLVLALLPVLLWLCCQGKALNFMALGEVQARQLGLSLHLWRN
LLVLAIGWLVGVSVALAGVIGFVGLVIPHMLRLSGLTNQRYLLPGCALAGAGVLLAADVVARITLLSAELPIGVVTATLG
APLFIWLLTRVKSLR
>Mature_334_residues
PASQTFTLLLHKQRHRDKRHLLLLTLGVAVAFVFSLSAGDVWLWPSQWSGEAAQLFVWQLRLPRALAVMLVGASLAVAGA
VMQSLFENPLAEPGLLGVANGAGVALVLTVLLGNGLLPVAFMSLSAIAGALVMTFLLLSFARRKRLTNARLLLVGVALGI
VCSAVMTWAVYFSSSLDLRQLMYWMMGGFGGVDWRQKWLVLALLPVLLWLCCQGKALNFMALGEVQARQLGLSLHLWRNL
LVLAIGWLVGVSVALAGVIGFVGLVIPHMLRLSGLTNQRYLLPGCALAGAGVLLAADVVARITLLSAELPIGVVTATLGA
PLFIWLLTRVKSLR

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Involved in the translocation of the substrate across the membrane

COG id: COG4139

COG function: function code H; ABC-type cobalamin transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily

Homologues:

Organism=Escherichia coli, GI1788004, Length=321, Percent_Identity=67.2897196261682, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1790740, Length=294, Percent_Identity=38.4353741496599, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1790741, Length=281, Percent_Identity=35.5871886120996, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1786805, Length=281, Percent_Identity=37.3665480427046, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786347, Length=331, Percent_Identity=31.1178247734139, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786804, Length=323, Percent_Identity=34.6749226006192, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BTUC_SERP5 (A8GDR2)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001478380.1
- ProteinModelPortal:   A8GDR2
- SMR:   A8GDR2
- STRING:   A8GDR2
- GeneID:   5605914
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_2151
- eggNOG:   COG4139
- HOGENOM:   HBG700016
- OMA:   CAGDQWI
- ProtClustDB:   PRK03784
- BioCyc:   SPRO399741:SPRO_2151-MONOMER
- HAMAP:   MF_01004
- InterPro:   IPR000522

Pfam domain/function: PF01032 FecCD

EC number: NA

Molecular weight: Translated: 35958; Mature: 35827

Theoretical pI: Translated: 11.41; Mature: 11.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x243c4378)-; HASH(0x250b7064)-; HASH(0x24965360)-; HASH(0x2542209c)-; HASH(0x24954b1c)-; HASH(0x2524de7c)-; HASH(0x40534348)-; HASH(0x2549740c)-; HASH(0x405696b8)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASQTFTLLLHKQRHRDKRHLLLLTLGVAVAFVFSLSAGDVWLWPSQWSGEAAQLFVWQ
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHH
LRLPRALAVMLVGASLAVAGAVMQSLFENPLAEPGLLGVANGAGVALVLTVLLGNGLLPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHCCCHHHH
AFMSLSAIAGALVMTFLLLSFARRKRLTNARLLLVGVALGIVCSAVMTWAVYFSSSLDLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
QLMYWMMGGFGGVDWRQKWLVLALLPVLLWLCCQGKALNFMALGEVQARQLGLSLHLWRN
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHH
LLVLAIGWLVGVSVALAGVIGFVGLVIPHMLRLSGLTNQRYLLPGCALAGAGVLLAADVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHH
ARITLLSAELPIGVVTATLGAPLFIWLLTRVKSLR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
PASQTFTLLLHKQRHRDKRHLLLLTLGVAVAFVFSLSAGDVWLWPSQWSGEAAQLFVWQ
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHH
LRLPRALAVMLVGASLAVAGAVMQSLFENPLAEPGLLGVANGAGVALVLTVLLGNGLLPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHCCCHHHH
AFMSLSAIAGALVMTFLLLSFARRKRLTNARLLLVGVALGIVCSAVMTWAVYFSSSLDLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
QLMYWMMGGFGGVDWRQKWLVLALLPVLLWLCCQGKALNFMALGEVQARQLGLSLHLWRN
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHH
LLVLAIGWLVGVSVALAGVIGFVGLVIPHMLRLSGLTNQRYLLPGCALAGAGVLLAADVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHH
ARITLLSAELPIGVVTATLGAPLFIWLLTRVKSLR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA