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The map label for this gene is btuE [H]

Identifier: 157370392

GI number: 157370392

Start: 2348419

End: 2348970

Strand: Direct

Name: btuE [H]

Synonym: Spro_2152

Alternate gene names: 157370392

Gene position: 2348419-2348970 (Clockwise)

Preceding gene: 157370391

Following gene: 157370393

Centisome position: 43.1

GC content: 54.71

Gene sequence:

>552_bases
ATGACTCAAGCGATTTATGACATTCCGTTGAACACTATCGAGAATCAATCGACCACGCTGGGCAAATATAAAGGCAGCGT
GTTGCTGGTGGTCAATGTGGCTTCGGAATGCGGCCTGACCAAGCAATATGCCGGGCTGGAAGCCCTGTACGAAACCTACC
GTGACCAGGGTTTTGAGGTATTGGGCTTCCCATCCAACGAGTTCCTCGGGCAGGAGCCTGGCAGCAACGAAGAGATCCTG
GCGTTTTGCCGTGGCACCTTTGGCGTTGAGTTCCCGATGTTTGCCAAGCTTGAAGTGAACAGCGAAAACCGTCATCCGCT
GTACCAGGCGCTGATTGCCGCCGAACCGCAAGCCGTGACGCCGCAGGACAGTGGATTCCTGGAGCGCATGAGCAGTAAAG
GCCGGGCACCGAAGCAAACCGGAGACATTCTGTGGAATTTTGAGAAATTCCTGATCGGGCGTGATGGCAATGTGATCCAA
CGTTTCTCTCCGGATATGACACCGGAAGATCCTGCGTTGGTGGCCGCCGTCAAACAGGCGTTGGCCAAGTAA

Upstream 100 bases:

>100_bases
CCACGCTGGGGGCACCGCTATTTATCTGGCTACTGACCCGGGTAAAAAGCTTAAGGTAGTTTCTCTGTTCACCTGCAAGT
AAAACAAGAGGATAAGCTCC

Downstream 100 bases:

>100_bases
TATAAAGAAGGAGCGATAATTCATAATGCTGCAACTCAGTCAGGTTGGGGTAGAAGGGCGTCTGGCCCCATTTTCAGCAC
AGGTCGATGCTGGGTTGCAG

Product: putative glutathione peroxidase

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MTQAIYDIPLNTIENQSTTLGKYKGSVLLVVNVASECGLTKQYAGLEALYETYRDQGFEVLGFPSNEFLGQEPGSNEEIL
AFCRGTFGVEFPMFAKLEVNSENRHPLYQALIAAEPQAVTPQDSGFLERMSSKGRAPKQTGDILWNFEKFLIGRDGNVIQ
RFSPDMTPEDPALVAAVKQALAK

Sequences:

>Translated_183_residues
MTQAIYDIPLNTIENQSTTLGKYKGSVLLVVNVASECGLTKQYAGLEALYETYRDQGFEVLGFPSNEFLGQEPGSNEEIL
AFCRGTFGVEFPMFAKLEVNSENRHPLYQALIAAEPQAVTPQDSGFLERMSSKGRAPKQTGDILWNFEKFLIGRDGNVIQ
RFSPDMTPEDPALVAAVKQALAK
>Mature_182_residues
TQAIYDIPLNTIENQSTTLGKYKGSVLLVVNVASECGLTKQYAGLEALYETYRDQGFEVLGFPSNEFLGQEPGSNEEILA
FCRGTFGVEFPMFAKLEVNSENRHPLYQALIAAEPQAVTPQDSGFLERMSSKGRAPKQTGDILWNFEKFLIGRDGNVIQR
FSPDMTPEDPALVAAVKQALAK

Specific function: Not essential for B12 transport; however, it is an auxiliary component of the transport system [H]

COG id: COG0386

COG function: function code O; Glutathione peroxidase

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutathione peroxidase family [H]

Homologues:

Organism=Homo sapiens, GI15618997, Length=184, Percent_Identity=37.5, Blast_Score=114, Evalue=5e-26,
Organism=Homo sapiens, GI90903240, Length=168, Percent_Identity=36.9047619047619, Blast_Score=107, Evalue=5e-24,
Organism=Homo sapiens, GI75709200, Length=168, Percent_Identity=36.9047619047619, Blast_Score=107, Evalue=6e-24,
Organism=Homo sapiens, GI4557629, Length=172, Percent_Identity=40.6976744186046, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI192455698, Length=183, Percent_Identity=31.1475409836066, Blast_Score=96, Evalue=1e-20,
Organism=Homo sapiens, GI6006001, Length=176, Percent_Identity=37.5, Blast_Score=92, Evalue=2e-19,
Organism=Homo sapiens, GI90903238, Length=150, Percent_Identity=36.6666666666667, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI41406084, Length=173, Percent_Identity=36.9942196531792, Blast_Score=90, Evalue=1e-18,
Organism=Homo sapiens, GI33186887, Length=162, Percent_Identity=37.037037037037, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI32967607, Length=171, Percent_Identity=32.7485380116959, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1788003, Length=183, Percent_Identity=62.2950819672131, Blast_Score=241, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17506887, Length=180, Percent_Identity=37.7777777777778, Blast_Score=124, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17535473, Length=168, Percent_Identity=39.8809523809524, Blast_Score=124, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI193209493, Length=188, Percent_Identity=35.1063829787234, Blast_Score=122, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17550320, Length=188, Percent_Identity=34.5744680851064, Blast_Score=120, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71993584, Length=168, Percent_Identity=33.9285714285714, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI193210707, Length=169, Percent_Identity=34.9112426035503, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71999642, Length=78, Percent_Identity=38.4615384615385, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71993573, Length=95, Percent_Identity=34.7368421052632, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319721, Length=183, Percent_Identity=40.9836065573771, Blast_Score=135, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6322228, Length=145, Percent_Identity=45.5172413793103, Blast_Score=129, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6322826, Length=160, Percent_Identity=34.375, Blast_Score=101, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24656772, Length=175, Percent_Identity=36, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24656777, Length=175, Percent_Identity=36, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24656767, Length=175, Percent_Identity=36, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI85726443, Length=151, Percent_Identity=37.0860927152318, Blast_Score=95, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000889
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00255 GSHPx [H]

EC number: NA

Molecular weight: Translated: 20201; Mature: 20070

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00460 GLUTATHIONE_PEROXID_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAIYDIPLNTIENQSTTLGKYKGSVLLVVNVASECGLTKQYAGLEALYETYRDQGFEV
CCCEEEECCCCCCCCCCCCHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEE
LGFPSNEFLGQEPGSNEEILAFCRGTFGVEFPMFAKLEVNSENRHPLYQALIAAEPQAVT
EECCCHHCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCC
PQDSGFLERMSSKGRAPKQTGDILWNFEKFLIGRDGNVIQRFSPDMTPEDPALVAAVKQA
CCCCHHHHHHHHCCCCCCCCCCEEEEHHHEEECCCCCCHHHCCCCCCCCCHHHHHHHHHH
LAK
HCC
>Mature Secondary Structure 
TQAIYDIPLNTIENQSTTLGKYKGSVLLVVNVASECGLTKQYAGLEALYETYRDQGFEV
CCEEEECCCCCCCCCCCCHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEE
LGFPSNEFLGQEPGSNEEILAFCRGTFGVEFPMFAKLEVNSENRHPLYQALIAAEPQAVT
EECCCHHCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCC
PQDSGFLERMSSKGRAPKQTGDILWNFEKFLIGRDGNVIQRFSPDMTPEDPALVAAVKQA
CCCCHHHHHHHHCCCCCCCCCCEEEEHHHEEECCCCCCHHHCCCCCCCCCHHHHHHHHHH
LAK
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3528129; 9097039; 9278503 [H]