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The map label for this gene is pheT [H]

Identifier: 157370389

GI number: 157370389

Start: 2344279

End: 2346666

Strand: Direct

Name: pheT [H]

Synonym: Spro_2149

Alternate gene names: 157370389

Gene position: 2344279-2346666 (Clockwise)

Preceding gene: 157370388

Following gene: 157370390

Centisome position: 43.02

GC content: 56.32

Gene sequence:

>2388_bases
ATGAAATTCAGTGAACTCTGGTTGCGCGAGTGGGTAAACCCGGCCATCAGCAGCGAAGCATTATCCGATCAAATTACCAT
GGCCGGCCTGGAAGTGGACGGCGTGGAGCCGGTAGCCGGTGCCTTTAATGGCGTCGTCGTGGGCCATGTGGTTGAGTGCG
GTCAGCATCCGAACGCCGACAAACTGCGCGTGACCAAGATCAACGTGGGCGGCGATCGCTTACTGGATATCGTCTGTGGT
GCACCAAACTGCCGTACCGGTCTGAAAGTGGCCGTTGCTACCGTGGGCGCGGTGCTGCCGGGCGATTTCAAAATCAAAGC
CGCCAAACTGCGCGGTGAGCCTTCGGAAGGGATGCTGTGCTCGTTCTCCGAGCTGGGTATTACTGACGATCACGACGGTA
TCATTGAGTTGCCGGAAGACGCACCGATTGGCACCGATATCCGTGAATATCTGAAGCTGGATGACAACGCGATCGAAATC
AGCGTAACGCCGAACCGCGCCGATTGCCTGGGCATTATTGGCGTGGCGCGTGACGTTGGGGTGCTGAACCAGGTTGCCCT
GACCGAACCGGACATGAGCCCGGTTGCTGCCACCATTAACGATACGCTGCCGATCCGCGTAGATGCGCCACAGGCGTGTC
CGCGTTACCTTGGCCGCGTAGTAAAAGGCATCGACGTCAAGGCCGCCAGCCCGTTGTGGATGCGTGAGAAGCTGCGTCGC
TGCGGCATTCGTTCTATAGATGCGGTGGTGGACGTTACCAACTACGTGCTGTTGGAACTGGGGCAGCCAATGCATGCCTT
CGACCTGAGCCGCATCGAAGGCGGTATCGTGGTGCGTATGGCCAACGTTGGGGAGCCTCTGACGCTGCTGGACGGTACCG
AAGCCAAACTGAATGCCGATACCTTAGTGATTGCCGATCACCAGAAAGCTCTGGCGATGGGTGGCATCTTCGGTGGTGAA
CACTCAGGGGTGAATGAATCCACCCAGGACGTGCTGCTGGAATGTGCGTTCTTCAACCCGCTGGCCATTACCGGCCGCGC
ACGCCGTCAGGGTCTGCACACTGATGCTTCCCATCGTTACGAGCGCGGTGTTGATCCGGCACTGCAGTACAAAGCGATGG
AGCGTGCGACTCGCCTGTTGATTGATATCTGTGGCGGCCAGGCCGGTCCGGTTATCGATGTAACCACCGAAAGTGAGTTG
CCGAAGCGCGCTACCATTACCTTACGTCGTGAAAAGCTCGATCGCCTGATTGGCCATGTGGTGCCAAGCGAGCAGGTCAG
TGACATTTTGCGTCGTCTGGGTTGCCAGGTGACCGAGCAGGGCGATAACTGGCTGGCGGTTGCGCCGAGCTGGCGTTTCG
ATATGGAAATAGAAGAAGACCTGGTGGAAGAAGTGGCGCGTGTTTACGGCTACAACAACATTCCTGACGTGCCGGTCCGC
GCTGACCTGATCATGACCAAGCATCGCGAAGCAGACCTGACCCTGAAACGCGTGAAGACCATGCTGGTCGATCACGGTTA
CCAGGAAGCGATCACCTACAGCTTCGTTGATCCTAAAGTACAGGCCTTGCTGCACCCAGGCGAAGAAGTGCTGATCCTGC
CAAGCCCAATCTCAGTAGAGATGTCCGCCATGCGTTTGTCGCTGTGGACCGGCTTGTTGTCCGCGGTGGTTTATAACCAG
AATCGCCAGCAGACGCGTCTGCGCCTGTTTGAAAGCGGCCTGCGCTTTGTCCCTGATACTGCGGCAAATCTGGGTATCCG
TCAGGACGTCATGCTGGCAGGGGTGATTGCGGGCCATACGCATGAAGAACATTGGGATCTGGCGCGTAAGCCAGTCGACT
TCTATGATTTAAAAGGGGATCTGGAGTCTGTCCTGGAGCTGACCGGTAAATTATCCGAAATTCAGTTCAAGGCAGAGGCT
AATCCGGCCCTGCACCCAGGGCAGAGCGCAGCGATTTATTTCCAGGGTGAACGTGTAGGTTTCATCGGTGTGGTTCATCC
GGAACTTGAACGTAAACTGGATCTTAACGGCCGCACAGTGGTGTTTGAACTGGAGTGGGACAAGGTCGCAAGCCGCGCCG
TACCTCAGGCGCGGGAGATTTCTCGCTTCCCGGCAAACCGCCGTGATATCGCCGTTGTGGTGGCTGAAAATGTCCCCGCA
GAAGATATTTTGGCAGAGTGTAAGAAAGTTGGCGCAAATCAGGTAGTTGGCGTAAACTTGTTTGATGTGTACCGTGGCAA
GGGCGTGGCAGAGGGTTATAAGAGCCTGGCTATCAGTCTGGTATTGCAGGATACCGCTCGTACACTGGAAGAAGAGGAGA
TAGCCGTTACCGTTGCAAAATGTGTAGAGGCTCTAAAACAGCGATTCCAAGCATCCTTGAGGGATTGA

Upstream 100 bases:

>100_bases
AGCGTCTGACGATGCTGCGTTACGGCGTTACCGACCTGCGTGCGTTCTTCGAAAACGATCTGCGCTTCCTCAAACAGTTT
AAGTAAGGCGGGAATATTAC

Downstream 100 bases:

>100_bases
ACCTATGGCGCTTACTAAAGCTGAAATGTCAGAACACCTGTTTGAAAAGCTTGGGCTTAGCAAACGGGATGCCAAAGACC
TGGTAGAACTGTTTTTCGAA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 795; Mature: 795

Protein sequence:

>795_residues
MKFSELWLREWVNPAISSEALSDQITMAGLEVDGVEPVAGAFNGVVVGHVVECGQHPNADKLRVTKINVGGDRLLDIVCG
APNCRTGLKVAVATVGAVLPGDFKIKAAKLRGEPSEGMLCSFSELGITDDHDGIIELPEDAPIGTDIREYLKLDDNAIEI
SVTPNRADCLGIIGVARDVGVLNQVALTEPDMSPVAATINDTLPIRVDAPQACPRYLGRVVKGIDVKAASPLWMREKLRR
CGIRSIDAVVDVTNYVLLELGQPMHAFDLSRIEGGIVVRMANVGEPLTLLDGTEAKLNADTLVIADHQKALAMGGIFGGE
HSGVNESTQDVLLECAFFNPLAITGRARRQGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGQAGPVIDVTTESEL
PKRATITLRREKLDRLIGHVVPSEQVSDILRRLGCQVTEQGDNWLAVAPSWRFDMEIEEDLVEEVARVYGYNNIPDVPVR
ADLIMTKHREADLTLKRVKTMLVDHGYQEAITYSFVDPKVQALLHPGEEVLILPSPISVEMSAMRLSLWTGLLSAVVYNQ
NRQQTRLRLFESGLRFVPDTAANLGIRQDVMLAGVIAGHTHEEHWDLARKPVDFYDLKGDLESVLELTGKLSEIQFKAEA
NPALHPGQSAAIYFQGERVGFIGVVHPELERKLDLNGRTVVFELEWDKVASRAVPQAREISRFPANRRDIAVVVAENVPA
EDILAECKKVGANQVVGVNLFDVYRGKGVAEGYKSLAISLVLQDTARTLEEEEIAVTVAKCVEALKQRFQASLRD

Sequences:

>Translated_795_residues
MKFSELWLREWVNPAISSEALSDQITMAGLEVDGVEPVAGAFNGVVVGHVVECGQHPNADKLRVTKINVGGDRLLDIVCG
APNCRTGLKVAVATVGAVLPGDFKIKAAKLRGEPSEGMLCSFSELGITDDHDGIIELPEDAPIGTDIREYLKLDDNAIEI
SVTPNRADCLGIIGVARDVGVLNQVALTEPDMSPVAATINDTLPIRVDAPQACPRYLGRVVKGIDVKAASPLWMREKLRR
CGIRSIDAVVDVTNYVLLELGQPMHAFDLSRIEGGIVVRMANVGEPLTLLDGTEAKLNADTLVIADHQKALAMGGIFGGE
HSGVNESTQDVLLECAFFNPLAITGRARRQGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGQAGPVIDVTTESEL
PKRATITLRREKLDRLIGHVVPSEQVSDILRRLGCQVTEQGDNWLAVAPSWRFDMEIEEDLVEEVARVYGYNNIPDVPVR
ADLIMTKHREADLTLKRVKTMLVDHGYQEAITYSFVDPKVQALLHPGEEVLILPSPISVEMSAMRLSLWTGLLSAVVYNQ
NRQQTRLRLFESGLRFVPDTAANLGIRQDVMLAGVIAGHTHEEHWDLARKPVDFYDLKGDLESVLELTGKLSEIQFKAEA
NPALHPGQSAAIYFQGERVGFIGVVHPELERKLDLNGRTVVFELEWDKVASRAVPQAREISRFPANRRDIAVVVAENVPA
EDILAECKKVGANQVVGVNLFDVYRGKGVAEGYKSLAISLVLQDTARTLEEEEIAVTVAKCVEALKQRFQASLRD
>Mature_795_residues
MKFSELWLREWVNPAISSEALSDQITMAGLEVDGVEPVAGAFNGVVVGHVVECGQHPNADKLRVTKINVGGDRLLDIVCG
APNCRTGLKVAVATVGAVLPGDFKIKAAKLRGEPSEGMLCSFSELGITDDHDGIIELPEDAPIGTDIREYLKLDDNAIEI
SVTPNRADCLGIIGVARDVGVLNQVALTEPDMSPVAATINDTLPIRVDAPQACPRYLGRVVKGIDVKAASPLWMREKLRR
CGIRSIDAVVDVTNYVLLELGQPMHAFDLSRIEGGIVVRMANVGEPLTLLDGTEAKLNADTLVIADHQKALAMGGIFGGE
HSGVNESTQDVLLECAFFNPLAITGRARRQGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGQAGPVIDVTTESEL
PKRATITLRREKLDRLIGHVVPSEQVSDILRRLGCQVTEQGDNWLAVAPSWRFDMEIEEDLVEEVARVYGYNNIPDVPVR
ADLIMTKHREADLTLKRVKTMLVDHGYQEAITYSFVDPKVQALLHPGEEVLILPSPISVEMSAMRLSLWTGLLSAVVYNQ
NRQQTRLRLFESGLRFVPDTAANLGIRQDVMLAGVIAGHTHEEHWDLARKPVDFYDLKGDLESVLELTGKLSEIQFKAEA
NPALHPGQSAAIYFQGERVGFIGVVHPELERKLDLNGRTVVFELEWDKVASRAVPQAREISRFPANRRDIAVVVAENVPA
EDILAECKKVGANQVVGVNLFDVYRGKGVAEGYKSLAISLVLQDTARTLEEEEIAVTVAKCVEALKQRFQASLRD

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=795, Percent_Identity=85.4088050314465, Blast_Score=1375, Evalue=0.0,
Organism=Drosophila melanogaster, GI21355211, Length=394, Percent_Identity=23.6040609137056, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 87099; Mature: 87099

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSELWLREWVNPAISSEALSDQITMAGLEVDGVEPVAGAFNGVVVGHVVECGQHPNAD
CCHHHHHHHHHCCHHHHHHHHHCCEEEEEEEECCCCHHHHHHCCCCEEHHHHCCCCCCCC
KLRVTKINVGGDRLLDIVCGAPNCRTGLKVAVATVGAVLPGDFKIKAAKLRGEPSEGMLC
CEEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEE
SFSELGITDDHDGIIELPEDAPIGTDIREYLKLDDNAIEISVTPNRADCLGIIGVARDVG
EHHHCCCCCCCCCEEECCCCCCCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHH
VLNQVALTEPDMSPVAATINDTLPIRVDAPQACPRYLGRVVKGIDVKAASPLWMREKLRR
HHHHHEECCCCCCCEEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
CGIRSIDAVVDVTNYVLLELGQPMHAFDLSRIEGGIVVRMANVGEPLTLLDGTEAKLNAD
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCEEEECCCCCEECCC
TLVIADHQKALAMGGIFGGEHSGVNESTQDVLLECAFFNPLAITGRARRQGLHTDASHRY
EEEEECCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCHHHHCCCCCCHHHHH
ERGVDPALQYKAMERATRLLIDICGGQAGPVIDVTTESELPKRATITLRREKLDRLIGHV
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEHHHHHHHHHHHC
VPSEQVSDILRRLGCQVTEQGDNWLAVAPSWRFDMEIEEDLVEEVARVYGYNNIPDVPVR
CCHHHHHHHHHHHCCEEEECCCCEEEECCCCEECCCHHHHHHHHHHHHCCCCCCCCCCCE
ADLIMTKHREADLTLKRVKTMLVDHGYQEAITYSFVDPKVQALLHPGEEVLILPSPISVE
EHEEEECCCCCCHHHHHHHHHHHHCCHHHHEEEEECCHHHHHHCCCCCEEEEECCCCCEE
MSAMRLSLWTGLLSAVVYNQNRQQTRLRLFESGLRFVPDTAANLGIRQDVMLAGVIAGHT
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHCCCCCHHHHHHHHHHCCC
HEEHWDLARKPVDFYDLKGDLESVLELTGKLSEIQFKAEANPALHPGQSAAIYFQGERVG
CHHHHHHHCCCCCCEECCHHHHHHHHHHCCHHHEEEEECCCCCCCCCCCEEEEEECCEEE
FIGVVHPELERKLDLNGRTVVFELEWDKVASRAVPQAREISRFPANRRDIAVVVAENVPA
EEEEECHHHHHHCCCCCCEEEEEEEHHHHHHHCCCCHHHHHHCCCCCCCEEEEEECCCCH
EDILAECKKVGANQVVGVNLFDVYRGKGVAEGYKSLAISLVLQDTARTLEEEEIAVTVAK
HHHHHHHHHCCCCCEEEEEHHHHHCCCCHHHHHHHHEEHEEEHHHHHHHHHHHHHHHHHH
CVEALKQRFQASLRD
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFSELWLREWVNPAISSEALSDQITMAGLEVDGVEPVAGAFNGVVVGHVVECGQHPNAD
CCHHHHHHHHHCCHHHHHHHHHCCEEEEEEEECCCCHHHHHHCCCCEEHHHHCCCCCCCC
KLRVTKINVGGDRLLDIVCGAPNCRTGLKVAVATVGAVLPGDFKIKAAKLRGEPSEGMLC
CEEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEE
SFSELGITDDHDGIIELPEDAPIGTDIREYLKLDDNAIEISVTPNRADCLGIIGVARDVG
EHHHCCCCCCCCCEEECCCCCCCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHH
VLNQVALTEPDMSPVAATINDTLPIRVDAPQACPRYLGRVVKGIDVKAASPLWMREKLRR
HHHHHEECCCCCCCEEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
CGIRSIDAVVDVTNYVLLELGQPMHAFDLSRIEGGIVVRMANVGEPLTLLDGTEAKLNAD
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCEEEECCCCCEECCC
TLVIADHQKALAMGGIFGGEHSGVNESTQDVLLECAFFNPLAITGRARRQGLHTDASHRY
EEEEECCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCHHHHCCCCCCHHHHH
ERGVDPALQYKAMERATRLLIDICGGQAGPVIDVTTESELPKRATITLRREKLDRLIGHV
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEHHHHHHHHHHHC
VPSEQVSDILRRLGCQVTEQGDNWLAVAPSWRFDMEIEEDLVEEVARVYGYNNIPDVPVR
CCHHHHHHHHHHHCCEEEECCCCEEEECCCCEECCCHHHHHHHHHHHHCCCCCCCCCCCE
ADLIMTKHREADLTLKRVKTMLVDHGYQEAITYSFVDPKVQALLHPGEEVLILPSPISVE
EHEEEECCCCCCHHHHHHHHHHHHCCHHHHEEEEECCHHHHHHCCCCCEEEEECCCCCEE
MSAMRLSLWTGLLSAVVYNQNRQQTRLRLFESGLRFVPDTAANLGIRQDVMLAGVIAGHT
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHCCCCCHHHHHHHHHHCCC
HEEHWDLARKPVDFYDLKGDLESVLELTGKLSEIQFKAEANPALHPGQSAAIYFQGERVG
CHHHHHHHCCCCCCEECCHHHHHHHHHHCCHHHEEEEECCCCCCCCCCCEEEEEECCEEE
FIGVVHPELERKLDLNGRTVVFELEWDKVASRAVPQAREISRFPANRRDIAVVVAENVPA
EEEEECHHHHHHCCCCCCEEEEEEEHHHHHHHCCCCHHHHHHCCCCCCCEEEEEECCCCH
EDILAECKKVGANQVVGVNLFDVYRGKGVAEGYKSLAISLVLQDTARTLEEEEIAVTVAK
HHHHHHHHHCCCCCEEEEEHHHHHCCCCHHHHHHHHEEHEEEHHHHHHHHHHHHHHHHHH
CVEALKQRFQASLRD
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]