Click here to switch to the map view.

The map label for this gene is maeN [H]

Identifier: 157370341

GI number: 157370341

Start: 2295530

End: 2296873

Strand: Direct

Name: maeN [H]

Synonym: Spro_2099

Alternate gene names: 157370341

Gene position: 2295530-2296873 (Clockwise)

Preceding gene: 157370335

Following gene: 157370342

Centisome position: 42.13

GC content: 55.43

Gene sequence:

>1344_bases
ATGAAACAGATAAATGCAGAAATGGCCTGTGATAAACAGGCGTTGTCGCCTGATTCATCAGGGCTGATTAATCAAATCCG
GAAAATGAATATCGGTGCCGTGCCGGTGGCGTTGTTTATTACCATTTGTGCCATTGTTGCCATTTCGGCTTATGCCAACT
TCTTGCCAAAGAATATGATCGGTGGCCTGGCGGTAATTATGACGCTGGGTTTTGCGCTGGCGCATTTGGGCAAAAGCATC
CCGGTGCTGCGTGATATCGGTGGGCCGGCGATTCTGTGTCTGATGGTGCCTTCGGTGCTGGTCTATTTTAATTTCTTCCA
GCCGAACGTGATGGGTACCGTTCATCTGCTGATGAAAGACGCCAACCTGCTGTATTTTGTTATCGCCAGCCTGGTGGTCG
GCAGCATTCTCGGCATGAACCGGGTGATCCTGATCCAGGGCATGATCCGCATGTTCGTGCCTTTGGTGGTCGGCACGGTG
ACGGCGGTGGTGACCGGGCTGCTGGTGGGCAAACTGTTTGGTTTTACCTTCTATCACACCTTCTTCTTTATCATTGTGCC
GATTATCGGTGGCGGTATCGGCGAGGGCATATTACCGCTGTCGCTGGCTTATTCAGCAATCCTTGGATCGACGCCGGACG
TGTACGTGGCGCAATTGGCACCGGCGGCGGTGCTGGGCAATATCTTCGCCATTGTGGCGGCAGGCGTATTGGCCCGCCTG
GGGATGAAGCGCAAGGCACTAAGCGGTGACGGTATGCTGATCCGGTCGGCGCAGGACAACGCGGTGTTTGCCATCAAGGA
AAGCACCGGCAACGTGGATTTCCAACTGATGGGCGGCGGGCTGCTGGTGATCTGCGCCTTCTTTATCGTCGGTGGGTTGT
TCGAACACATCGTCCACATTCCCGGCCCGGTGCTGATGATTCTGTTTGCGGTACTGTGCAAATATTGCCGGGTGATCCCG
ACCTCAATGGAAACCGGTGCGCACAGTTTTTATAAGTTTGTTTCAACCGCGCTGGTGTGGCCGCTGATGATTGGCCTGGG
CATGCTGTACGTACCGCTGGAAAGCGTGGTGGCGGTGTTCTCGGTAGGCTATGTGGTGGTCTGCGGTTCGGTGGTGCTGT
CGATGGGGCTGGTGAGCTTCCTGATTGCGCCATACCTGAAAATGTACCCGGTTGAAGCGGCGATCGTCACCTGCTGCCAC
AGTGGGTTAGGTGGGACTGGCGACGTGGCGATCCTATCCGCGTCCAACCGTATGGGGTTAATGCCGTTTGCGCAAATCGC
CACGCGCATCGGCGGTGCGTCCACAGTGATTGGCGCCACGCTATTGTTGGGTTGGTTAATCTGA

Upstream 100 bases:

>100_bases
CCCCTCATCGTTTTATGATGGAATTTTTTAACCGTTTAAGTTATTTCATAAATCGATGGGCGATCCGCTCCGTACTGAAC
ACTTGTCAGCAGGGTGAACA

Downstream 100 bases:

>100_bases
GTCCAGACTGAATTATGGATACCGGGGTGGATTGCCCCGGTTTTTTTATTTATGTCATCGATTAATAATAAGCATTGAAA
TGGAATCAGAATTGCCGAGA

Product: citrate carrier protein

Products: NA

Alternate protein names: Sodium-dependent malate transporter [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKQINAEMACDKQALSPDSSGLINQIRKMNIGAVPVALFITICAIVAISAYANFLPKNMIGGLAVIMTLGFALAHLGKSI
PVLRDIGGPAILCLMVPSVLVYFNFFQPNVMGTVHLLMKDANLLYFVIASLVVGSILGMNRVILIQGMIRMFVPLVVGTV
TAVVTGLLVGKLFGFTFYHTFFFIIVPIIGGGIGEGILPLSLAYSAILGSTPDVYVAQLAPAAVLGNIFAIVAAGVLARL
GMKRKALSGDGMLIRSAQDNAVFAIKESTGNVDFQLMGGGLLVICAFFIVGGLFEHIVHIPGPVLMILFAVLCKYCRVIP
TSMETGAHSFYKFVSTALVWPLMIGLGMLYVPLESVVAVFSVGYVVVCGSVVLSMGLVSFLIAPYLKMYPVEAAIVTCCH
SGLGGTGDVAILSASNRMGLMPFAQIATRIGGASTVIGATLLLGWLI

Sequences:

>Translated_447_residues
MKQINAEMACDKQALSPDSSGLINQIRKMNIGAVPVALFITICAIVAISAYANFLPKNMIGGLAVIMTLGFALAHLGKSI
PVLRDIGGPAILCLMVPSVLVYFNFFQPNVMGTVHLLMKDANLLYFVIASLVVGSILGMNRVILIQGMIRMFVPLVVGTV
TAVVTGLLVGKLFGFTFYHTFFFIIVPIIGGGIGEGILPLSLAYSAILGSTPDVYVAQLAPAAVLGNIFAIVAAGVLARL
GMKRKALSGDGMLIRSAQDNAVFAIKESTGNVDFQLMGGGLLVICAFFIVGGLFEHIVHIPGPVLMILFAVLCKYCRVIP
TSMETGAHSFYKFVSTALVWPLMIGLGMLYVPLESVVAVFSVGYVVVCGSVVLSMGLVSFLIAPYLKMYPVEAAIVTCCH
SGLGGTGDVAILSASNRMGLMPFAQIATRIGGASTVIGATLLLGWLI
>Mature_447_residues
MKQINAEMACDKQALSPDSSGLINQIRKMNIGAVPVALFITICAIVAISAYANFLPKNMIGGLAVIMTLGFALAHLGKSI
PVLRDIGGPAILCLMVPSVLVYFNFFQPNVMGTVHLLMKDANLLYFVIASLVVGSILGMNRVILIQGMIRMFVPLVVGTV
TAVVTGLLVGKLFGFTFYHTFFFIIVPIIGGGIGEGILPLSLAYSAILGSTPDVYVAQLAPAAVLGNIFAIVAAGVLARL
GMKRKALSGDGMLIRSAQDNAVFAIKESTGNVDFQLMGGGLLVICAFFIVGGLFEHIVHIPGPVLMILFAVLCKYCRVIP
TSMETGAHSFYKFVSTALVWPLMIGLGMLYVPLESVVAVFSVGYVVVCGSVVLSMGLVSFLIAPYLKMYPVEAAIVTCCH
SGLGGTGDVAILSASNRMGLMPFAQIATRIGGASTVIGATLLLGWLI

Specific function: Acts as a Na(+)-malate symporter, as it catalyzes malate-dependent uptake of Na(+) and Na(+)-dependent uptake of malate [H]

COG id: COG3493

COG function: function code C; Na+/citrate symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:citrate (SCF) symporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004679
- InterPro:   IPR018025 [H]

Pfam domain/function: PF03390 2HCT [H]

EC number: NA

Molecular weight: Translated: 47062; Mature: 47062

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQINAEMACDKQALSPDSSGLINQIRKMNIGAVPVALFITICAIVAISAYANFLPKNMI
CCCCCCHHHCCHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHH
GGLAVIMTLGFALAHLGKSIPVLRDIGGPAILCLMVPSVLVYFNFFQPNVMGTVHLLMKD
HHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
ANLLYFVIASLVVGSILGMNRVILIQGMIRMFVPLVVGTVTAVVTGLLVGKLFGFTFYHT
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFFIIVPIIGGGIGEGILPLSLAYSAILGSTPDVYVAQLAPAAVLGNIFAIVAAGVLARL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMKRKALSGDGMLIRSAQDNAVFAIKESTGNVDFQLMGGGLLVICAFFIVGGLFEHIVHI
CCHHHCCCCCCEEEEECCCCEEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHC
PGPVLMILFAVLCKYCRVIPTSMETGAHSFYKFVSTALVWPLMIGLGMLYVPLESVVAVF
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVGYVVVCGSVVLSMGLVSFLIAPYLKMYPVEAAIVTCCHSGLGGTGDVAILSASNRMGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
MPFAQIATRIGGASTVIGATLLLGWLI
CHHHHHHHHHCCHHHHHHHHHHHHHHC
>Mature Secondary Structure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CHHHHHHHHHCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10903309; 12949159 [H]