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The map label for this gene is pcaQ [H]

Identifier: 157370335

GI number: 157370335

Start: 2287916

End: 2289142

Strand: Direct

Name: pcaQ [H]

Synonym: Spro_2093

Alternate gene names: 157370335

Gene position: 2287916-2289142 (Clockwise)

Preceding gene: 157370323

Following gene: 157370341

Centisome position: 41.99

GC content: 61.37

Gene sequence:

>1227_bases
ATGGACTATGATGAGAATATGCTGACAAACCTGATGCAAATCAGGGCATTCAGTCAGGTAGTGATCCAGGGAAGCGTCTC
ACGCGCAGCCGATGAGCTATTCCGAACCCAGTCAGCAGTCACCCGCTCAATCCGCGATTTGGAGCAGCAGCTGGCGGCAC
CCTTGTTTGAACGCCATGCCAGCGGCATGCTGCTGACTGACTTTGGCAAATGCGTGCTGCCGCGCGCCCGGCGGGCGATT
GATGAATTGCACCAGATACCGGCGCTGCTAAAGCGCTTACAGGGCAAGGGGGGCCAGACGCGCGGCGATCCCGAACCCTT
GTACCTGTTCAATGTGCGACGACTGCAGATTTTCGTCTGCCTGTGTGAAACCCGGCATATGCAAACCGTCGCCACGCTGC
TCGGACTAAGCCAGCCGGCGATCAGTGCCGCGCTCAAGGTGCTGGAAAACGGTGCCGGGGTGCCATTGCTGGAGCGCACC
CCGCAGGGGATGGTACCGTCGCTGGCCGGCCGTGAAATCGAGGCTAACGTTCGCCGTGCGCTGAATGAACTGCAACATAT
TCCCGCGGATATCGCTGCCCGCCGCGGGGTGCTGGAGGGGCGAGTGCGGGTGGGGGCATTACCGTTGGGACGTACCCGGC
TGTTGCCGCAGGCCATCATCCAACTGACCTCCCGCTATCCGGGCGTCAGCGTGGTGACCAACGAAAGTGCCTTTGCCGCC
CTGGCCACCGAGCTGCGTTCCGGGGATATCGATTTTATTTTTGGTGCTCTGCGCGCCAGCGATTATGCCGCCGATGTGTA
CAGTGAAACGCTGTTTACCGAGGATATGGTGCTGCTGGCGCGCCGCGATCACCCACTGGCGACACCAGGCTTAAGGCCTG
AACAGCTGAGAGCGGCGCGCTGGGTCTTACCACGCTCCGCCACGCCGGCGCGGCGCTTGCTGGACGATTGCTTCCTGGCG
ATGAATATTCCGGTACCCAATCCGGTGGTGGAAACCGGCGATTTGGCATTGGTGAGGGGTTTGCTGCTGAATTCAGACAT
GCTGGCGGCGGTGTCAGCCCACCAGTTGGAATATGAGCTGGCCTCCGGTGAACTGGTTCAGTTACCGATAGCGCTGCCCA
ATACCGGACGCGCCATCGGTCTGAGTTGCCGCACCGGCAGCCTGCACTCCCCGGCGGCGCAGGCGCTGATCGCCTGCCTG
CGGGAAGTCTGTGTGGTTAACCTGTAG

Upstream 100 bases:

>100_bases
CTTCATGCTGTCAGCTCAGGCGGGTGACGGATAATTCATTTGATTGCAGCAGGTATGCGTTTTATTTATATTGCCAGCCA
CGGGAAAAGGAGATGACGCG

Downstream 100 bases:

>100_bases
CGATTATAACCGTGCCGCCTGCATCAGCGCGTACCGGTATCCCTGAATGCCAAAGCCGCAAATCACGCCGTTGGCCACGT
CGGACAGGTAGGAATGATGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Pca operon transcriptional activator [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MDYDENMLTNLMQIRAFSQVVIQGSVSRAADELFRTQSAVTRSIRDLEQQLAAPLFERHASGMLLTDFGKCVLPRARRAI
DELHQIPALLKRLQGKGGQTRGDPEPLYLFNVRRLQIFVCLCETRHMQTVATLLGLSQPAISAALKVLENGAGVPLLERT
PQGMVPSLAGREIEANVRRALNELQHIPADIAARRGVLEGRVRVGALPLGRTRLLPQAIIQLTSRYPGVSVVTNESAFAA
LATELRSGDIDFIFGALRASDYAADVYSETLFTEDMVLLARRDHPLATPGLRPEQLRAARWVLPRSATPARRLLDDCFLA
MNIPVPNPVVETGDLALVRGLLLNSDMLAAVSAHQLEYELASGELVQLPIALPNTGRAIGLSCRTGSLHSPAAQALIACL
REVCVVNL

Sequences:

>Translated_408_residues
MDYDENMLTNLMQIRAFSQVVIQGSVSRAADELFRTQSAVTRSIRDLEQQLAAPLFERHASGMLLTDFGKCVLPRARRAI
DELHQIPALLKRLQGKGGQTRGDPEPLYLFNVRRLQIFVCLCETRHMQTVATLLGLSQPAISAALKVLENGAGVPLLERT
PQGMVPSLAGREIEANVRRALNELQHIPADIAARRGVLEGRVRVGALPLGRTRLLPQAIIQLTSRYPGVSVVTNESAFAA
LATELRSGDIDFIFGALRASDYAADVYSETLFTEDMVLLARRDHPLATPGLRPEQLRAARWVLPRSATPARRLLDDCFLA
MNIPVPNPVVETGDLALVRGLLLNSDMLAAVSAHQLEYELASGELVQLPIALPNTGRAIGLSCRTGSLHSPAAQALIACL
REVCVVNL
>Mature_408_residues
MDYDENMLTNLMQIRAFSQVVIQGSVSRAADELFRTQSAVTRSIRDLEQQLAAPLFERHASGMLLTDFGKCVLPRARRAI
DELHQIPALLKRLQGKGGQTRGDPEPLYLFNVRRLQIFVCLCETRHMQTVATLLGLSQPAISAALKVLENGAGVPLLERT
PQGMVPSLAGREIEANVRRALNELQHIPADIAARRGVLEGRVRVGALPLGRTRLLPQAIIQLTSRYPGVSVVTNESAFAA
LATELRSGDIDFIFGALRASDYAADVYSETLFTEDMVLLARRDHPLATPGLRPEQLRAARWVLPRSATPARRLLDDCFLA
MNIPVPNPVVETGDLALVRGLLLNSDMLAAVSAHQLEYELASGELVQLPIALPNTGRAIGLSCRTGSLHSPAAQALIACL
REVCVVNL

Specific function: Activates transcription of the pcaDCHGB operon for the catabolism of the phenolic compound protocatechuate [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081904, Length=291, Percent_Identity=26.1168384879725, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788748, Length=225, Percent_Identity=32.4444444444444, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787601, Length=303, Percent_Identity=27.0627062706271, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790399, Length=181, Percent_Identity=31.4917127071823, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693105, Length=300, Percent_Identity=24.6666666666667, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789506, Length=258, Percent_Identity=23.2558139534884, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1788481, Length=273, Percent_Identity=25.6410256410256, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788887, Length=183, Percent_Identity=30.6010928961749, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR012787
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 44504; Mature: 44504

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYDENMLTNLMQIRAFSQVVIQGSVSRAADELFRTQSAVTRSIRDLEQQLAAPLFERHA
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGMLLTDFGKCVLPRARRAIDELHQIPALLKRLQGKGGQTRGDPEPLYLFNVRRLQIFVC
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHH
LCETRHMQTVATLLGLSQPAISAALKVLENGAGVPLLERTPQGMVPSLAGREIEANVRRA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHH
LNELQHIPADIAARRGVLEGRVRVGALPLGRTRLLPQAIIQLTSRYPGVSVVTNESAFAA
HHHHHHCCHHHHHHCCHHHCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHH
LATELRSGDIDFIFGALRASDYAADVYSETLFTEDMVLLARRDHPLATPGLRPEQLRAAR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHH
WVLPRSATPARRLLDDCFLAMNIPVPNPVVETGDLALVRGLLLNSDMLAAVSAHQLEYEL
HCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHEE
ASGELVQLPIALPNTGRAIGLSCRTGSLHSPAAQALIACLREVCVVNL
CCCCEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDYDENMLTNLMQIRAFSQVVIQGSVSRAADELFRTQSAVTRSIRDLEQQLAAPLFERHA
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGMLLTDFGKCVLPRARRAIDELHQIPALLKRLQGKGGQTRGDPEPLYLFNVRRLQIFVC
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHH
LCETRHMQTVATLLGLSQPAISAALKVLENGAGVPLLERTPQGMVPSLAGREIEANVRRA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHH
LNELQHIPADIAARRGVLEGRVRVGALPLGRTRLLPQAIIQLTSRYPGVSVVTNESAFAA
HHHHHHCCHHHHHHCCHHHCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHH
LATELRSGDIDFIFGALRASDYAADVYSETLFTEDMVLLARRDHPLATPGLRPEQLRAAR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHH
WVLPRSATPARRLLDDCFLAMNIPVPNPVVETGDLALVRGLLLNSDMLAAVSAHQLEYEL
HCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHEE
ASGELVQLPIALPNTGRAIGLSCRTGSLHSPAAQALIACLREVCVVNL
CCCCEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]