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The map label for this gene is proP [H]

Identifier: 157370326

GI number: 157370326

Start: 2278832

End: 2280142

Strand: Reverse

Name: proP [H]

Synonym: Spro_2084

Alternate gene names: 157370326

Gene position: 2280142-2278832 (Counterclockwise)

Preceding gene: 157370327

Following gene: 157370325

Centisome position: 41.85

GC content: 61.02

Gene sequence:

>1311_bases
ATGAGTACACCAACGACGGTGATTGACGCACGTCAGTCGCGGCAGGCATTACTGGCCGGATCGGTCGGCAACTTTATCGA
GTGGTATGAATTTGGCGTTTACGGTTTTCTCGCCACCGTGATCGCCGCCAACTTTTTCAGCCTGCAGGGCGAAAGCGAAA
TCACCAGCCTGATCCTCACCTACGCCGCCTTTGCACTGGCCTTTTTCTGTCGGCCGATCGGTGCGGTGATTTTCGGCCGC
ATCGGCGATCGCATTGGTCGCCGACCGACGCTGATTGCGGTATTGCTGCTGATGACGGTAGCCACGGCGCTGATCGGCGT
GATGCCCACCTATGCCTCTATTGGCGTCGCCGCCCCGCTGTTGCTGACCCTGTTGCGTATGTTCCAGGGGCTGTTTGCCG
GCGGTGAGTTCGGCGGCGCAGTGTCGCTGATGACCGAGTTCGCGCCCAAGGGCAAGCGCGGCCTGTTCGGTGCCTGGCAA
TCGCTGACCGTCGCGTTCGGGCTGCTGGCCGGTGCCGGGTTGGTGGCGCTGTTGGCCGCGTTATTGACGCCGGAACAGCT
GCACGACTGGGGCTGGCGCATTCCCTTCCTGCTGGCACTGCCGATGGGCGCGGTGGCATTGTGGCTGCGGTTGAAACTGC
AGGAAACGCCAACCTTTACCCAGGCACAGCAAACCCGTAGCTCTACGACGCAGCCACCAGCGGTAAAACTGAGCGGCGTA
GCAAAAACCATTGCTCTGGGCATTGGCCGCATGATGGGCTGGTCCGCCGCCGGTTACACTTTTTTGGTCGTCATGCCCTC
CTATCTGCAAACCTCGCTGCACGCCACCTTCCAGCAGGCACTGGTGGCCACGGTGCTGGCCAACATCGGCTTCGCGCTGA
CCATTTTGCCCGCCGGTATCCTTAGCGATAAGCTGGGACGCAAAACGGTGATGCTGACGGCCGTGGTGGCGGTTATCCTG
TTTACCTTCCCGCTGCTGCACCTGTTGCAGGACCCGCAAAGTTCACTGCTGACCAAAGGCATCGCGGTGATGATTGCCGG
TGCGGTGGTCGGCTTGCTGGCCGGCCCCGGCCCGGCGATGCTGGCAGAAATGTTCCCCACCCAGGTTCGTTATACCGGTT
TGGGGCTGGCCTACTCGCTGTCCAATGCGGTGTTCTCAGGCTCCGCTGGGCTAATTATCACCGGGCTTATCAAGCAGACC
GGCAACCTCGATATCCCGGCCTACTACGTGGTAGCCACCTCAGTGGTCAGCCTGTTCGCGCTGATGACCCTGCGCCGCGA
CGATCATCTGCGTTCACTTAACGAGTCTTGA

Upstream 100 bases:

>100_bases
CCTGGTGATCCCCCCTCACAGTTCCCGCTGAAAATAATTGATTGTTTTGCCATAAGAATTTAGAACGGAGTAACAATAAT
TCAAATACCGGGGAATGGGG

Downstream 100 bases:

>100_bases
TCCCTTTGGGGCGCCCAGCGCGCCCTGCTTCCCCTTCGGTGATTTCGATCACACTCGCAAAATGTTAACGTTAACTTTTG
TGATTAACATCGCAATCCCT

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSTPTTVIDARQSRQALLAGSVGNFIEWYEFGVYGFLATVIAANFFSLQGESEITSLILTYAAFALAFFCRPIGAVIFGR
IGDRIGRRPTLIAVLLLMTVATALIGVMPTYASIGVAAPLLLTLLRMFQGLFAGGEFGGAVSLMTEFAPKGKRGLFGAWQ
SLTVAFGLLAGAGLVALLAALLTPEQLHDWGWRIPFLLALPMGAVALWLRLKLQETPTFTQAQQTRSSTTQPPAVKLSGV
AKTIALGIGRMMGWSAAGYTFLVVMPSYLQTSLHATFQQALVATVLANIGFALTILPAGILSDKLGRKTVMLTAVVAVIL
FTFPLLHLLQDPQSSLLTKGIAVMIAGAVVGLLAGPGPAMLAEMFPTQVRYTGLGLAYSLSNAVFSGSAGLIITGLIKQT
GNLDIPAYYVVATSVVSLFALMTLRRDDHLRSLNES

Sequences:

>Translated_436_residues
MSTPTTVIDARQSRQALLAGSVGNFIEWYEFGVYGFLATVIAANFFSLQGESEITSLILTYAAFALAFFCRPIGAVIFGR
IGDRIGRRPTLIAVLLLMTVATALIGVMPTYASIGVAAPLLLTLLRMFQGLFAGGEFGGAVSLMTEFAPKGKRGLFGAWQ
SLTVAFGLLAGAGLVALLAALLTPEQLHDWGWRIPFLLALPMGAVALWLRLKLQETPTFTQAQQTRSSTTQPPAVKLSGV
AKTIALGIGRMMGWSAAGYTFLVVMPSYLQTSLHATFQQALVATVLANIGFALTILPAGILSDKLGRKTVMLTAVVAVIL
FTFPLLHLLQDPQSSLLTKGIAVMIAGAVVGLLAGPGPAMLAEMFPTQVRYTGLGLAYSLSNAVFSGSAGLIITGLIKQT
GNLDIPAYYVVATSVVSLFALMTLRRDDHLRSLNES
>Mature_435_residues
STPTTVIDARQSRQALLAGSVGNFIEWYEFGVYGFLATVIAANFFSLQGESEITSLILTYAAFALAFFCRPIGAVIFGRI
GDRIGRRPTLIAVLLLMTVATALIGVMPTYASIGVAAPLLLTLLRMFQGLFAGGEFGGAVSLMTEFAPKGKRGLFGAWQS
LTVAFGLLAGAGLVALLAALLTPEQLHDWGWRIPFLLALPMGAVALWLRLKLQETPTFTQAQQTRSSTTQPPAVKLSGVA
KTIALGIGRMMGWSAAGYTFLVVMPSYLQTSLHATFQQALVATVLANIGFALTILPAGILSDKLGRKTVMLTAVVAVILF
TFPLLHLLQDPQSSLLTKGIAVMIAGAVVGLLAGPGPAMLAEMFPTQVRYTGLGLAYSLSNAVFSGSAGLIITGLIKQTG
NLDIPAYYVVATSVVSLFALMTLRRDDHLRSLNES

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=432, Percent_Identity=33.7962962962963, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1788942, Length=437, Percent_Identity=35.6979405034325, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788292, Length=434, Percent_Identity=32.0276497695853, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1789941, Length=444, Percent_Identity=33.1081081081081, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI87082404, Length=373, Percent_Identity=24.9329758713137, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87082231, Length=200, Percent_Identity=26.5, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46192; Mature: 46061

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STPTTVIDARQSRQALLAGSVGNFIEWYEFGVYGFLATVIAANFFSLQGESEITSLILT
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
YAAFALAFFCRPIGAVIFGRIGDRIGRRPTLIAVLLLMTVATALIGVMPTYASIGVAAPL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLTLLRMFQGLFAGGEFGGAVSLMTEFAPKGKRGLFGAWQSLTVAFGLLAGAGLVALLAA
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLTPEQLHDWGWRIPFLLALPMGAVALWLRLKLQETPTFTQAQQTRSSTTQPPAVKLSGV
HHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEHHHH
AKTIALGIGRMMGWSAAGYTFLVVMPSYLQTSLHATFQQALVATVLANIGFALTILPAGI
HHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSDKLGRKTVMLTAVVAVILFTFPLLHLLQDPQSSLLTKGIAVMIAGAVVGLLAGPGPAM
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LAEMFPTQVRYTGLGLAYSLSNAVFSGSAGLIITGLIKQTGNLDIPAYYVVATSVVSLFA
HHHHCCHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LMTLRRDDHLRSLNES
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]