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The map label for this gene is lacY [H]

Identifier: 157370325

GI number: 157370325

Start: 2277399

End: 2278658

Strand: Reverse

Name: lacY [H]

Synonym: Spro_2083

Alternate gene names: 157370325

Gene position: 2278658-2277399 (Counterclockwise)

Preceding gene: 157370326

Following gene: 157370324

Centisome position: 41.82

GC content: 57.3

Gene sequence:

>1260_bases
ATGAACCGCGAAACAAAAAAATATTATGTGCTTCTCAGCGGCCTGTTGTTTTTCTTCTTCTTTACCTGGTCATCCAGCTT
TTCACTGATCTCCATCTGGCTGAACCAGAAAATCGGCCTGAAAGGGACTGAAACCGGGCTGATCTTCGCGGCAATGTCGA
TCATGGCGTTGTGCGCCCAACCGCTGTACGGCTTTATTCAGGACAAACTTGGGCTGCGTAAGCACCTGCTGCTGTTTGTC
GGCGTGCTGCTGTTGCTCACCGGCCCGTTCTTTATCTATGTCTACGCCCCGCTGCTGCAGAGCAACCTTGTGGTCGGCGC
ACTGGTGGGCGGCGTGTTTGTCAGCCTGGCGTTCAATGCCGGTATTGGCGCGCTGGAATCCTATACCGAACGAGTCAGCC
GCATCGTCGGTTTCGAATTCGGCCGGGCGCGTATGTGGGGGTCATTGGGCTGGGCCAGCGCCACCTTCTTTGCCGGCTTT
AACTACAATATCGACCCCAATATCAACTTCTGGATCGCTTCGGCCTCGGCGGCAGTGTTTCTGCTGTTGCTGTGGCAAGT
GCGTGAGCTGAAACCCAACGCCATGGCCGGTCTGGAATACGGCAAGCCGGAAAACCTGAAGCTGCAGGACGCATTGGCCC
TGCTGCGCCTGCCGGGGTTCTGGGCGCTGGTGGTGTTTGTGCTGGGCACCAGCATCTACGGCGTGTTTGACCAGCAGTTC
CCGGTGTATTTCGCCTCGCAGTTCCCCACCCACGAAGAAGGCAACCGCATGTACGGTTTCCTTAATTCGCTGCAGGTGTT
TCTGGAGGCCGGTGGCATGTTCCTGGCCCCGCTGCTGGTTAACCGCATTGGCATAAAGCAAAGCCTGTTGCTGGCCAGCA
GCGTGATGGCGCTGCGCATGGTCGGTTCCGGCTTTGCCAGCGGCGCCCTGATGATTTCCGCCATGAAACTGCTGCACGCC
GTAGAATTGCCAATCCTGCTGGTGGCGATGTTCAAGTACATCACCACCCGTTTCGACAGCCGCCTGTCCTCCACGCTGTA
CCTGGTGGGCTTCCAGTTTATCAGCCAAATTGTCGCCGGTTTTCTGGCACCGCTGGCCGGTTATGGTTACGACCGCATCG
GCTTTGCCGACACCTATTTGCTGATGGGTTGCGCGGTGGCCGGGACCACGCTGATTTCCTGCTTCCTGCTGCGCGGCGAG
ACCGTCGCCAGTGCGCCTCAATTTCAATCCACGTTAAAATCAAGTGAGCCAACCCAATGA

Upstream 100 bases:

>100_bases
ACTTTTGTGATTAACATCGCAATCCCTTACTTTGATGGCTTTATCTGGTTTAAAGTTAACGTTAACAATAGTGCCAATTT
CATCCATCAGGGCATTCCAC

Downstream 100 bases:

>100_bases
AAAATGCCTTAGCTCAAGCCGATCATGCGGTCGAAGCCCTGCGCGCTCAGCGTCAGGATCTCTACTACCCGCGCTTTCAT
CTGGCCCCGGCCGCCGGCTG

Product: galactoside permease

Products: Proton [Cytoplasm]; lactose [Cytoplasm] [C]

Alternate protein names: Lactose-proton symport [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MNRETKKYYVLLSGLLFFFFFTWSSSFSLISIWLNQKIGLKGTETGLIFAAMSIMALCAQPLYGFIQDKLGLRKHLLLFV
GVLLLLTGPFFIYVYAPLLQSNLVVGALVGGVFVSLAFNAGIGALESYTERVSRIVGFEFGRARMWGSLGWASATFFAGF
NYNIDPNINFWIASASAAVFLLLLWQVRELKPNAMAGLEYGKPENLKLQDALALLRLPGFWALVVFVLGTSIYGVFDQQF
PVYFASQFPTHEEGNRMYGFLNSLQVFLEAGGMFLAPLLVNRIGIKQSLLLASSVMALRMVGSGFASGALMISAMKLLHA
VELPILLVAMFKYITTRFDSRLSSTLYLVGFQFISQIVAGFLAPLAGYGYDRIGFADTYLLMGCAVAGTTLISCFLLRGE
TVASAPQFQSTLKSSEPTQ

Sequences:

>Translated_419_residues
MNRETKKYYVLLSGLLFFFFFTWSSSFSLISIWLNQKIGLKGTETGLIFAAMSIMALCAQPLYGFIQDKLGLRKHLLLFV
GVLLLLTGPFFIYVYAPLLQSNLVVGALVGGVFVSLAFNAGIGALESYTERVSRIVGFEFGRARMWGSLGWASATFFAGF
NYNIDPNINFWIASASAAVFLLLLWQVRELKPNAMAGLEYGKPENLKLQDALALLRLPGFWALVVFVLGTSIYGVFDQQF
PVYFASQFPTHEEGNRMYGFLNSLQVFLEAGGMFLAPLLVNRIGIKQSLLLASSVMALRMVGSGFASGALMISAMKLLHA
VELPILLVAMFKYITTRFDSRLSSTLYLVGFQFISQIVAGFLAPLAGYGYDRIGFADTYLLMGCAVAGTTLISCFLLRGE
TVASAPQFQSTLKSSEPTQ
>Mature_419_residues
MNRETKKYYVLLSGLLFFFFFTWSSSFSLISIWLNQKIGLKGTETGLIFAAMSIMALCAQPLYGFIQDKLGLRKHLLLFV
GVLLLLTGPFFIYVYAPLLQSNLVVGALVGGVFVSLAFNAGIGALESYTERVSRIVGFEFGRARMWGSLGWASATFFAGF
NYNIDPNINFWIASASAAVFLLLLWQVRELKPNAMAGLEYGKPENLKLQDALALLRLPGFWALVVFVLGTSIYGVFDQQF
PVYFASQFPTHEEGNRMYGFLNSLQVFLEAGGMFLAPLLVNRIGIKQSLLLASSVMALRMVGSGFASGALMISAMKLLHA
VELPILLVAMFKYITTRFDSRLSSTLYLVGFQFISQIVAGFLAPLAGYGYDRIGFADTYLLMGCAVAGTTLISCFLLRGE
TVASAPQFQSTLKSSEPTQ

Specific function: Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family [H]

Homologues:

Organism=Escherichia coli, GI1786538, Length=376, Percent_Identity=43.6170212765958, Blast_Score=312, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR000576
- InterPro:   IPR018457
- InterPro:   IPR020846
- InterPro:   IPR016196 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 46076; Mature: 46076

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRETKKYYVLLSGLLFFFFFTWSSSFSLISIWLNQKIGLKGTETGLIFAAMSIMALCAQ
CCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
PLYGFIQDKLGLRKHLLLFVGVLLLLTGPFFIYVYAPLLQSNLVVGALVGGVFVSLAFNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
GIGALESYTERVSRIVGFEFGRARMWGSLGWASATFFAGFNYNIDPNINFWIASASAAVF
CCHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHEECCCCCCCCCCEEEEECHHHHHH
LLLLWQVRELKPNAMAGLEYGKPENLKLQDALALLRLPGFWALVVFVLGTSIYGVFDQQF
HHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
PVYFASQFPTHEEGNRMYGFLNSLQVFLEAGGMFLAPLLVNRIGIKQSLLLASSVMALRM
CCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHH
VGSGFASGALMISAMKLLHAVELPILLVAMFKYITTRFDSRLSSTLYLVGFQFISQIVAG
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLAPLAGYGYDRIGFADTYLLMGCAVAGTTLISCFLLRGETVASAPQFQSTLKSSEPTQ
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MNRETKKYYVLLSGLLFFFFFTWSSSFSLISIWLNQKIGLKGTETGLIFAAMSIMALCAQ
CCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
PLYGFIQDKLGLRKHLLLFVGVLLLLTGPFFIYVYAPLLQSNLVVGALVGGVFVSLAFNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
GIGALESYTERVSRIVGFEFGRARMWGSLGWASATFFAGFNYNIDPNINFWIASASAAVF
CCHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHEECCCCCCCCCCEEEEECHHHHHH
LLLLWQVRELKPNAMAGLEYGKPENLKLQDALALLRLPGFWALVVFVLGTSIYGVFDQQF
HHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
PVYFASQFPTHEEGNRMYGFLNSLQVFLEAGGMFLAPLLVNRIGIKQSLLLASSVMALRM
CCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHH
VGSGFASGALMISAMKLLHAVELPILLVAMFKYITTRFDSRLSSTLYLVGFQFISQIVAG
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLAPLAGYGYDRIGFADTYLLMGCAVAGTTLISCFLLRGETVASAPQFQSTLKSSEPTQ
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; lactose [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactose [Periplasm] = Proton [Cytoplasm] + lactose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3056524 [H]