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The map label for this gene is cscA [H]

Identifier: 157370324

GI number: 157370324

Start: 2275960

End: 2277402

Strand: Reverse

Name: cscA [H]

Synonym: Spro_2082

Alternate gene names: 157370324

Gene position: 2277402-2275960 (Counterclockwise)

Preceding gene: 157370325

Following gene: 157370320

Centisome position: 41.8

GC content: 60.5

Gene sequence:

>1443_bases
ATGAAAAATGCCTTAGCTCAAGCCGATCATGCGGTCGAAGCCCTGCGCGCTCAGCGTCAGGATCTCTACTACCCGCGCTT
TCATCTGGCCCCGGCCGCCGGCTGGATCAACGATCCCAACGGCCTGGTGTGTATTAACGGCGTTTATCACGCTTTCTACC
AGCACCACCCTTACGACCAGAACTGGGGGCCGATGCACTGGGGCCATGCCACCAGCCGGGATCTGGCGCACTGGCAGCAC
CAGCCGATCGCTCTGGCCCCCGGTGACGACTATGACAAGGACGGCTGTTTCTCCGGCTGCGCAGTAGATGATAACGGCGT
ACTGACGCTGCTCTATACCGGCCACGTCTGGCTGGGTAAAGCCGGTGATGACGATCAGGTGCGCGAGGTTCAGTGCCTGG
CCACCAGCGAGGATGGGATCCATTTTGTTAAACACGGGCCGGTGCTGTTGCCGCCGGAAGGCATTCAACACTTTCGCGAT
CCAAAAGTCTGGCGCGCCGGTGACTGCTGGTGGATGGTGGTCGGAGCCAAAGAGAACGGGCTGGGACAGGCGCGCCTTTA
TCGCTCCAGCGACCTGCGCGACTGGCAGTTTGACCGCGTGCTGAGCGGAGCGCAAACGCTGAGTCAGGGCTTTATGTGGG
AGTGCCCGGACTTCTTCCCGCTGGGCGAAAAACAGGTTCTGTTGTTTTCACCACAGGGGCTGGCGGCCCAGGGTTACCGG
AATCGCAACCGCTTCCAGAGTGGCTATTTGCTCGGCCACTGGCAGCCGGGTGAGGATTATGGCATCACACAGCCCTTCTG
TGAGCTGGACAGCGGGCACGATTTCTATGCGCCGCAAAGCTTCGCCGCCGCCGATGGCCGTCGCATGCTGTTTGCCTGGA
TGGACATGTGGGAATCTCCGATGCCGAGTAAAGCGCACGGCTGGGCCGGTGCACTGACCCTGCCGCGTGAATTGACGCTG
GCAACCGATGGCAATGTACTGATGAACCCGGCGCGGGAGCTGACGGCGTTGCGCGGTCAGCAACATTCGTTTGCTGCGGT
CACATTGAGCAACCAACGGCAAGCTCTGGACGGCGAAGTGCAAGAATTGGCGCTGACGCTGAACCTGGCCAACAGCAATG
CCGAGCGCTATGGCATCACTATCGGCAAAGCGGCAAGGCTGTACGTCGACAATCAGGCCCATCGCTTGGTGTTAGAGAGG
TTTAGTGAGGAGCCAGGGCTGTGCGGTTGTCGCAGCGTTCCCCTACCGGAGGGGGACGAACTTTCGTTGCGAGTCTTTAT
TGATCGTTCATCGCTGGAGGTGTTCGTCAATCAGGGTGTGGCCTGTCTGACCAGCCGCATCTACCCGGCCGACGGTCAGC
GCGGCGTTAGCCTGTTTGCCGAAGGCGGACAGGCGCAGTTTGCTGCATCACAGGGCTGGGAGCTGGAGAGTATCTGGGAC
TGA

Upstream 100 bases:

>100_bases
TGGCCGGGACCACGCTGATTTCCTGCTTCCTGCTGCGCGGCGAGACCGTCGCCAGTGCGCCTCAATTTCAATCCACGTTA
AAATCAAGTGAGCCAACCCA

Downstream 100 bases:

>100_bases
CCTTTTTTTACAGGCTATTGGGAATGTCCGCTATTAGGGAGAGCGTGCCGATGGGGTCGTAGCGGCTTGTCCGCCGACCA
ATTTGCCGGGAGCAAATTGG

Product: sucrose-6-phosphate hydrolase

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MKNALAQADHAVEALRAQRQDLYYPRFHLAPAAGWINDPNGLVCINGVYHAFYQHHPYDQNWGPMHWGHATSRDLAHWQH
QPIALAPGDDYDKDGCFSGCAVDDNGVLTLLYTGHVWLGKAGDDDQVREVQCLATSEDGIHFVKHGPVLLPPEGIQHFRD
PKVWRAGDCWWMVVGAKENGLGQARLYRSSDLRDWQFDRVLSGAQTLSQGFMWECPDFFPLGEKQVLLFSPQGLAAQGYR
NRNRFQSGYLLGHWQPGEDYGITQPFCELDSGHDFYAPQSFAAADGRRMLFAWMDMWESPMPSKAHGWAGALTLPRELTL
ATDGNVLMNPARELTALRGQQHSFAAVTLSNQRQALDGEVQELALTLNLANSNAERYGITIGKAARLYVDNQAHRLVLER
FSEEPGLCGCRSVPLPEGDELSLRVFIDRSSLEVFVNQGVACLTSRIYPADGQRGVSLFAEGGQAQFAASQGWELESIWD

Sequences:

>Translated_480_residues
MKNALAQADHAVEALRAQRQDLYYPRFHLAPAAGWINDPNGLVCINGVYHAFYQHHPYDQNWGPMHWGHATSRDLAHWQH
QPIALAPGDDYDKDGCFSGCAVDDNGVLTLLYTGHVWLGKAGDDDQVREVQCLATSEDGIHFVKHGPVLLPPEGIQHFRD
PKVWRAGDCWWMVVGAKENGLGQARLYRSSDLRDWQFDRVLSGAQTLSQGFMWECPDFFPLGEKQVLLFSPQGLAAQGYR
NRNRFQSGYLLGHWQPGEDYGITQPFCELDSGHDFYAPQSFAAADGRRMLFAWMDMWESPMPSKAHGWAGALTLPRELTL
ATDGNVLMNPARELTALRGQQHSFAAVTLSNQRQALDGEVQELALTLNLANSNAERYGITIGKAARLYVDNQAHRLVLER
FSEEPGLCGCRSVPLPEGDELSLRVFIDRSSLEVFVNQGVACLTSRIYPADGQRGVSLFAEGGQAQFAASQGWELESIWD
>Mature_480_residues
MKNALAQADHAVEALRAQRQDLYYPRFHLAPAAGWINDPNGLVCINGVYHAFYQHHPYDQNWGPMHWGHATSRDLAHWQH
QPIALAPGDDYDKDGCFSGCAVDDNGVLTLLYTGHVWLGKAGDDDQVREVQCLATSEDGIHFVKHGPVLLPPEGIQHFRD
PKVWRAGDCWWMVVGAKENGLGQARLYRSSDLRDWQFDRVLSGAQTLSQGFMWECPDFFPLGEKQVLLFSPQGLAAQGYR
NRNRFQSGYLLGHWQPGEDYGITQPFCELDSGHDFYAPQSFAAADGRRMLFAWMDMWESPMPSKAHGWAGALTLPRELTL
ATDGNVLMNPARELTALRGQQHSFAAVTLSNQRQALDGEVQELALTLNLANSNAERYGITIGKAARLYVDNQAHRLVLER
FSEEPGLCGCRSVPLPEGDELSLRVFIDRSSLEVFVNQGVACLTSRIYPADGQRGVSLFAEGGQAQFAASQGWELESIWD

Specific function: Enables the bacterium to metabolize sucrose as a sole carbon source [H]

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=497, Percent_Identity=25.9557344064386, Blast_Score=127, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 53557; Mature: 53557

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNALAQADHAVEALRAQRQDLYYPRFHLAPAAGWINDPNGLVCINGVYHAFYQHHPYDQ
CCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEECHHHHHHHHCCCCCC
NWGPMHWGHATSRDLAHWQHQPIALAPGDDYDKDGCFSGCAVDDNGVLTLLYTGHVWLGK
CCCCCCCCCCCCHHHHHHCCCCEEECCCCCCCCCCCCCCCEECCCCEEEEEEECEEEEEC
AGDDDQVREVQCLATSEDGIHFVKHGPVLLPPEGIQHFRDPKVWRAGDCWWMVVGAKENG
CCCCCHHEEEEEEEECCCCEEEEECCCEEECCHHHHHCCCCCCEECCCEEEEEEECCCCC
LGQARLYRSSDLRDWQFDRVLSGAQTLSQGFMWECPDFFPLGEKQVLLFSPQGLAAQGYR
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEECCCCCCCCCCC
NRNRFQSGYLLGHWQPGEDYGITQPFCELDSGHDFYAPQSFAAADGRRMLFAWMDMWESP
CCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCCCCCCCCCHHCCCCCEEEEEHHHHHCCC
MPSKAHGWAGALTLPRELTLATDGNVLMNPARELTALRGQQHSFAAVTLSNQRQALDGEV
CCCCCCCCEEEEECCCEEEEEECCCEEECCHHHHHHHCCCCCEEEEEEECCCCHHHCCCC
QELALTLNLANSNAERYGITIGKAARLYVDNQAHRLVLERFSEEPGLCGCRSVPLPEGDE
EEEEEEEEECCCCCCEEEEEECCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LSLRVFIDRSSLEVFVNQGVACLTSRIYPADGQRGVSLFAEGGQAQFAASQGWELESIWD
EEEEEEEECCCEEEEECCCHHHHHHCCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKNALAQADHAVEALRAQRQDLYYPRFHLAPAAGWINDPNGLVCINGVYHAFYQHHPYDQ
CCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEECHHHHHHHHCCCCCC
NWGPMHWGHATSRDLAHWQHQPIALAPGDDYDKDGCFSGCAVDDNGVLTLLYTGHVWLGK
CCCCCCCCCCCCHHHHHHCCCCEEECCCCCCCCCCCCCCCEECCCCEEEEEEECEEEEEC
AGDDDQVREVQCLATSEDGIHFVKHGPVLLPPEGIQHFRDPKVWRAGDCWWMVVGAKENG
CCCCCHHEEEEEEEECCCCEEEEECCCEEECCHHHHHCCCCCCEECCCEEEEEEECCCCC
LGQARLYRSSDLRDWQFDRVLSGAQTLSQGFMWECPDFFPLGEKQVLLFSPQGLAAQGYR
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEECCCCCCCCCCC
NRNRFQSGYLLGHWQPGEDYGITQPFCELDSGHDFYAPQSFAAADGRRMLFAWMDMWESP
CCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCCCCCCCCCHHCCCCCEEEEEHHHHHCCC
MPSKAHGWAGALTLPRELTLATDGNVLMNPARELTALRGQQHSFAAVTLSNQRQALDGEV
CCCCCCCCEEEEECCCEEEEEECCCEEECCHHHHHHHCCCCCEEEEEEECCCCHHHCCCC
QELALTLNLANSNAERYGITIGKAARLYVDNQAHRLVLERFSEEPGLCGCRSVPLPEGDE
EEEEEEEEECCCCCCEEEEEECCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LSLRVFIDRSSLEVFVNQGVACLTSRIYPADGQRGVSLFAEGGQAQFAASQGWELESIWD
EEEEEEEECCCEEEEECCCHHHHHHCCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218016 [H]