Click here to switch to the map view.

The map label for this gene is pepT

Identifier: 157370251

GI number: 157370251

Start: 2200946

End: 2202175

Strand: Direct

Name: pepT

Synonym: Spro_2009

Alternate gene names: 157370251

Gene position: 2200946-2202175 (Clockwise)

Preceding gene: 157370248

Following gene: 157370252

Centisome position: 40.39

GC content: 54.39

Gene sequence:

>1230_bases
ATGGATAAACTACTAGATCGATTTTTTAACTACGTCTCATTCGACACGCAATCCAAAGCGAATGTCAAACATGTGCCGAG
TACCGACGGGCAAATGAAGCTGGCGCGCGCGTTGCAGCAAGAAATGATTGAACTGGGGTTTGAACGGGTGTCGCTCAGCG
AGCATGGCTGCGTGATGGGCACTTTACCGGGCAACGTTGCCTGGTCCGTACCGGCTATCGGGTTTATTTCTCACCTGGAT
ACCTCACCGGATTTTACCGGCAAACACGTTAATCCGCAGATCGTCGAAAGTTACCGCGGTGGCGATATTGCATTGGGCAT
TGGCGATGAGGTGTTATCGCCGGTGATGTTCCCGATCCTGCACCAGATGCTGGGGCAGACGTTGATCACTACCGACGGCA
AAACGCTGCTCGGTGCCGATGACAAGGCAGGCATTGCCGAAATTCTGACCGCCATGGTGCGGTTGCAGCAACGCAATATC
CCCCACGGCGATATTCGCGTGGCTTTTACTCCGGACGAGGAAGTGGGCAAGGGCGCACGATTTTTTGACGTTGCACAATT
CAACGCCGAGTGGGCCTATACCGTAGATGGCGGTGGTGTCGGTGAGTTGGAATGCGAGAACTTCAATGCGGCGTCGGTGA
CGGTGAAAATTGTCGGCAATAATGTTCACCCTGGGAGCGCTAAAGGGGTGATGGTGAATGCCCTGTCACTGGCAACCCGT
ATTCAGCAGGCGTTACCGGCGGATGAAACCCCGGAAACCACCGCCGATTATCAGGGTTTTTATCATCTCAGCAGCCTCAA
GGGCAGCGTCGAGCGCGCCGAAATGCACTATATTCTGCGTGATTTCGAGCGTGAGGGTTTCGAGGCCCGCAAGCGCCGGA
TGTTTGAGGTGGCCAAGCAGGTGGGCAAAGGTCTGCCGCGGGATTGTTATATCGAAGTGACGATTGAAGACAGTTACTAC
AACATGCGCGAACAGGTGGCAGAACATCCCCATGTGATTGCGTTGGCGCAACAGGCCATGCGCGATTGCGATATTGAACC
AGTGATGAAGCCGATTCGCGGCGGTACCGACGGCGCTCAGTTATCGTTTCGCGGTCTGCCTTGTCCGAATCTGTTTACCG
GCGGTTATAACTATCATTGCAAGCATGAGTTTGTTTCCCTTGAAGGGATGGAGCAAGCAGTGGCCGTGATTATGCGCATC
GCTGCGCTGACGGCAGAACGCGCCAAATAG

Upstream 100 bases:

>100_bases
TTTAATGATTTCCGTTAAATCTGGCTAAAAATGACCTGTCGCAATATTTGATCCTGATCGGTTCCGCATAATGAAACGCG
ATATTCTGAGGGCGGATACC

Downstream 100 bases:

>100_bases
CCCCGATTGCGATTATTATAATTGCTTGCCGTAACAATGATTTGCTCCTAGTGTAGAACATATTTTCCGCACCAGGAGCC
GTATCATGAGCACTGAACAC

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MDKLLDRFFNYVSFDTQSKANVKHVPSTDGQMKLARALQQEMIELGFERVSLSEHGCVMGTLPGNVAWSVPAIGFISHLD
TSPDFTGKHVNPQIVESYRGGDIALGIGDEVLSPVMFPILHQMLGQTLITTDGKTLLGADDKAGIAEILTAMVRLQQRNI
PHGDIRVAFTPDEEVGKGARFFDVAQFNAEWAYTVDGGGVGELECENFNAASVTVKIVGNNVHPGSAKGVMVNALSLATR
IQQALPADETPETTADYQGFYHLSSLKGSVERAEMHYILRDFEREGFEARKRRMFEVAKQVGKGLPRDCYIEVTIEDSYY
NMREQVAEHPHVIALAQQAMRDCDIEPVMKPIRGGTDGAQLSFRGLPCPNLFTGGYNYHCKHEFVSLEGMEQAVAVIMRI
AALTAERAK

Sequences:

>Translated_409_residues
MDKLLDRFFNYVSFDTQSKANVKHVPSTDGQMKLARALQQEMIELGFERVSLSEHGCVMGTLPGNVAWSVPAIGFISHLD
TSPDFTGKHVNPQIVESYRGGDIALGIGDEVLSPVMFPILHQMLGQTLITTDGKTLLGADDKAGIAEILTAMVRLQQRNI
PHGDIRVAFTPDEEVGKGARFFDVAQFNAEWAYTVDGGGVGELECENFNAASVTVKIVGNNVHPGSAKGVMVNALSLATR
IQQALPADETPETTADYQGFYHLSSLKGSVERAEMHYILRDFEREGFEARKRRMFEVAKQVGKGLPRDCYIEVTIEDSYY
NMREQVAEHPHVIALAQQAMRDCDIEPVMKPIRGGTDGAQLSFRGLPCPNLFTGGYNYHCKHEFVSLEGMEQAVAVIMRI
AALTAERAK
>Mature_409_residues
MDKLLDRFFNYVSFDTQSKANVKHVPSTDGQMKLARALQQEMIELGFERVSLSEHGCVMGTLPGNVAWSVPAIGFISHLD
TSPDFTGKHVNPQIVESYRGGDIALGIGDEVLSPVMFPILHQMLGQTLITTDGKTLLGADDKAGIAEILTAMVRLQQRNI
PHGDIRVAFTPDEEVGKGARFFDVAQFNAEWAYTVDGGGVGELECENFNAASVTVKIVGNNVHPGSAKGVMVNALSLATR
IQQALPADETPETTADYQGFYHLSSLKGSVERAEMHYILRDFEREGFEARKRRMFEVAKQVGKGLPRDCYIEVTIEDSYY
NMREQVAEHPHVIALAQQAMRDCDIEPVMKPIRGGTDGAQLSFRGLPCPNLFTGGYNYHCKHEFVSLEGMEQAVAVIMRI
AALTAERAK

Specific function: Cleaves the N-terminal amino acid of tripeptides

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family

Homologues:

Organism=Escherichia coli, GI1787372, Length=407, Percent_Identity=76.6584766584767, Blast_Score=668, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEPT_SERP5 (A8GDC2)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001478240.1
- ProteinModelPortal:   A8GDC2
- SMR:   A8GDC2
- STRING:   A8GDC2
- MEROPS:   M20.003
- GeneID:   5603212
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_2009
- eggNOG:   COG2195
- HOGENOM:   HBG288638
- OMA:   HMDTAPD
- ProtClustDB:   PRK05469
- BioCyc:   SPRO399741:SPRO_2009-MONOMER
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00550
- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161
- PIRSF:   PIRSF037215
- TIGRFAMs:   TIGR01882

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.4.11.4

Molecular weight: Translated: 45055; Mature: 45055

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2; PS00079 MULTICOPPER_OXIDASE1

Important sites: ACT_SITE 80-80 ACT_SITE 173-173

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLLDRFFNYVSFDTQSKANVKHVPSTDGQMKLARALQQEMIELGFERVSLSEHGCVMG
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHCCCCCCCEEEE
TLPGNVAWSVPAIGFISHLDTSPDFTGKHVNPQIVESYRGGDIALGIGDEVLSPVMFPIL
CCCCCCEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCEEEEECCHHHHHHHHHHHH
HQMLGQTLITTDGKTLLGADDKAGIAEILTAMVRLQQRNIPHGDIRVAFTPDEEVGKGAR
HHHHCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHCCCCC
FFDVAQFNAEWAYTVDGGGVGELECENFNAASVTVKIVGNNVHPGSAKGVMVNALSLATR
EEEEEECCCCEEEEECCCCCCCEEECCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHH
IQQALPADETPETTADYQGFYHLSSLKGSVERAEMHYILRDFEREGFEARKRRMFEVAKQ
HHHHCCCCCCCCCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VGKGLPRDCYIEVTIEDSYYNMREQVAEHPHVIALAQQAMRDCDIEPVMKPIRGGTDGAQ
HHCCCCCCEEEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCE
LSFRGLPCPNLFTGGYNYHCKHEFVSLEGMEQAVAVIMRIAALTAERAK
EEECCCCCCCCCCCCCCEEECCCEEECCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDKLLDRFFNYVSFDTQSKANVKHVPSTDGQMKLARALQQEMIELGFERVSLSEHGCVMG
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHCCCCCCCEEEE
TLPGNVAWSVPAIGFISHLDTSPDFTGKHVNPQIVESYRGGDIALGIGDEVLSPVMFPIL
CCCCCCEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCEEEEECCHHHHHHHHHHHH
HQMLGQTLITTDGKTLLGADDKAGIAEILTAMVRLQQRNIPHGDIRVAFTPDEEVGKGAR
HHHHCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHCCCCC
FFDVAQFNAEWAYTVDGGGVGELECENFNAASVTVKIVGNNVHPGSAKGVMVNALSLATR
EEEEEECCCCEEEEECCCCCCCEEECCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHH
IQQALPADETPETTADYQGFYHLSSLKGSVERAEMHYILRDFEREGFEARKRRMFEVAKQ
HHHHCCCCCCCCCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VGKGLPRDCYIEVTIEDSYYNMREQVAEHPHVIALAQQAMRDCDIEPVMKPIRGGTDGAQ
HHCCCCCCEEEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCE
LSFRGLPCPNLFTGGYNYHCKHEFVSLEGMEQAVAVIMRIAALTAERAK
EEECCCCCCCCCCCCCCEEECCCEEECCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA