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The map label for this gene is lolB

Identifier: 157370230

GI number: 157370230

Start: 2175710

End: 2176333

Strand: Reverse

Name: lolB

Synonym: Spro_1988

Alternate gene names: 157370230

Gene position: 2176333-2175710 (Counterclockwise)

Preceding gene: 157370237

Following gene: 157370229

Centisome position: 39.94

GC content: 57.21

Gene sequence:

>624_bases
ATGCCAATCCGCAAAGTTAGTTTGCTCCGCCTGATCCCGCTGGCCAGCCTGGTGCTGGCCGCCTGTACTACCACCAAGCC
GACCGGCCCGGCCACCAGCCCGACGTCGCCGCAGTGGCGTGCCCACGAACAAGCGGTGCAACAGCTGAGTCAGTATCAGA
CCCGTGGTTCTTTCGCCTACCTGTCAGATCAGAAAAAAGTCTACGCCCGCTTCTTCTGGCAACAGTATTCCCCAGACCGT
TATCGCCTGCTGCTGACCAACCCGCTTGGCAGTACCGAGCTTGACCTCAACGTACAGAAAAACGTAGTGCAGTTGACGGA
TAACCAGGGCAAACGTTACGTCAGCGACAATGCCGAAGAGATGATCCGCAAGCTGACCGGCATGGCCATTCCGCTGGACA
ACCTGCGCCAGTGGATGCTCGGCCTGCCGGGTGAGGCCAGCGACTTCAAGCTCGATGACCAATATCGCCTGAACTCGCTG
ACCTACCAACAAGGTAGCCAGACCTGGACCGTGGATTACCAGGACTACAACAACAGCCTCAAACCACAGTTGCCCAGCCG
CCTGGAACTGAAACAGGGTGACCAGCGCATCAAACTGAAAATGGACGATTGGACGCTTAAATGA

Upstream 100 bases:

>100_bases
CAGCAAAAAATTGACGAGCCGGCGTTGCCCCGCTAGCGTAAATGCTGATACCAAGGTCTCGCTGTGCGTGCCCGCAAGGA
CAAAAGGACAAGAGAAAGTT

Downstream 100 bases:

>100_bases
TTCGTCAATGGCCATCGCCCGCTAAACTCAATTTGTTTTTGTACATTACCGGCCAACGTGAAGACGGCTACCACCTGCTG
CAGACGCTGTTCCAGTTTCT

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MPIRKVSLLRLIPLASLVLAACTTTKPTGPATSPTSPQWRAHEQAVQQLSQYQTRGSFAYLSDQKKVYARFFWQQYSPDR
YRLLLTNPLGSTELDLNVQKNVVQLTDNQGKRYVSDNAEEMIRKLTGMAIPLDNLRQWMLGLPGEASDFKLDDQYRLNSL
TYQQGSQTWTVDYQDYNNSLKPQLPSRLELKQGDQRIKLKMDDWTLK

Sequences:

>Translated_207_residues
MPIRKVSLLRLIPLASLVLAACTTTKPTGPATSPTSPQWRAHEQAVQQLSQYQTRGSFAYLSDQKKVYARFFWQQYSPDR
YRLLLTNPLGSTELDLNVQKNVVQLTDNQGKRYVSDNAEEMIRKLTGMAIPLDNLRQWMLGLPGEASDFKLDDQYRLNSL
TYQQGSQTWTVDYQDYNNSLKPQLPSRLELKQGDQRIKLKMDDWTLK
>Mature_206_residues
PIRKVSLLRLIPLASLVLAACTTTKPTGPATSPTSPQWRAHEQAVQQLSQYQTRGSFAYLSDQKKVYARFFWQQYSPDRY
RLLLTNPLGSTELDLNVQKNVVQLTDNQGKRYVSDNAEEMIRKLTGMAIPLDNLRQWMLGLPGEASDFKLDDQYRLNSLT
YQQGSQTWTVDYQDYNNSLKPQLPSRLELKQGDQRIKLKMDDWTLK

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family

Homologues:

Organism=Escherichia coli, GI1787460, Length=207, Percent_Identity=63.768115942029, Blast_Score=286, Evalue=7e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LOLB_SERP5 (A8GDA1)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001478219.1
- ProteinModelPortal:   A8GDA1
- SMR:   A8GDA1
- STRING:   A8GDA1
- GeneID:   5603229
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1988
- eggNOG:   COG3017
- HOGENOM:   HBG644390
- OMA:   WLQRQDY
- ProtClustDB:   PRK00022
- BioCyc:   SPRO399741:SPRO_1988-MONOMER
- HAMAP:   MF_00233
- InterPro:   IPR004565
- TIGRFAMs:   TIGR00548

Pfam domain/function: PF03550 LolB

EC number: NA

Molecular weight: Translated: 23889; Mature: 23758

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIRKVSLLRLIPLASLVLAACTTTKPTGPATSPTSPQWRAHEQAVQQLSQYQTRGSFAY
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
LSDQKKVYARFFWQQYSPDRYRLLLTNPLGSTELDLNVQKNVVQLTDNQGKRYVSDNAEE
ECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCEEEEECCCCCEEECCCHHH
MIRKLTGMAIPLDNLRQWMLGLPGEASDFKLDDQYRLNSLTYQQGSQTWTVDYQDYNNSL
HHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHCEEEECCCCEEEEEEHHCCCCCC
KPQLPSRLELKQGDQRIKLKMDDWTLK
CCCCCCCCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
PIRKVSLLRLIPLASLVLAACTTTKPTGPATSPTSPQWRAHEQAVQQLSQYQTRGSFAY
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
LSDQKKVYARFFWQQYSPDRYRLLLTNPLGSTELDLNVQKNVVQLTDNQGKRYVSDNAEE
ECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCEEEEECCCCCEEECCCHHH
MIRKLTGMAIPLDNLRQWMLGLPGEASDFKLDDQYRLNSLTYQQGSQTWTVDYQDYNNSL
HHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHCEEEECCCCEEEEEEHHCCCCCC
KPQLPSRLELKQGDQRIKLKMDDWTLK
CCCCCCCCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA