Click here to switch to the map view.

The map label for this gene is ispE

Identifier: 157370229

GI number: 157370229

Start: 2174847

End: 2175713

Strand: Reverse

Name: ispE

Synonym: Spro_1987

Alternate gene names: 157370229

Gene position: 2175713-2174847 (Counterclockwise)

Preceding gene: 157370230

Following gene: 157370228

Centisome position: 39.93

GC content: 57.09

Gene sequence:

>867_bases
ATGATTCGTCAATGGCCATCGCCCGCTAAACTCAATTTGTTTTTGTACATTACCGGCCAACGTGAAGACGGCTACCACCT
GCTGCAGACGCTGTTCCAGTTTCTGGATTACGGCGACACGCTGACCATCGACGTGCGCCAGGATGACGCCATTCACCTGC
TGACCCCGGTGGCCGGCGTGCCGGATGAGCAAAACCTGATCGTCCGCGCCGCCCGTTTGCTGCAACAGCATTGCGATCGT
CATGGGCTAAACACCGCCCCGCGCGGTGCCGAGATCAGCATCGAAAAACGTCTGCCGATGGGTGGCGGACTGGGCGGCGG
TTCCTCCAACGCCGCCACGGTGTTAGTGGCGCTCAATGATCTGTGGCGCTGCGGCTTGAGCGACGAGCAGTTGGCAGAGC
TGGGGCTGGCCCTTGGTGCCGATGTTCCGGTGTTCGTTCGCGGCCATGCCGCCTTTGCCGAGGGCATCGGTGAACGTTTG
CAACCTGCCAATCCGCAGGAAAAGTGGTATCTGGTGGCCCATCCGGGTGTCAGCATCCCGACCCCGGTTATTTTTGGCGA
TGCCGATTTAAAAAGAGACACCCCGGTTCGCCCATTAAATGTGCTGTTACAGGCAACTTACGCAAATGATTGCGAACCAA
TAGCAAGAAAACGTTTTCGCGAGGTTGAACAGCTTGTTTCTTGGCTGTTAGAATACGCGCCGTCACGCCTGACTGGCACA
GGTGCTTGTGTGTTTGCTGAATTCGACACAGAAACCGCCGCCCGTCAGGTGTTAAATCAAGCCCCGGAGTGGATACGTGG
TTTTGTAGCGCGTGGCGTTAACGTCTCTCCACTACATCGCATTCGTTCCGGGCAGATTGAACCGTAG

Upstream 100 bases:

>100_bases
AGGACTACAACAACAGCCTCAAACCACAGTTGCCCAGCCGCCTGGAACTGAAACAGGGTGACCAGCGCATCAAACTGAAA
ATGGACGATTGGACGCTTAA

Downstream 100 bases:

>100_bases
TTCTCGTTTCGCTGCGCAGAGTCGCTCACACCTGGCCCTGTCATCCCTGCGCCGCTGCGGTTTCAAATTTTTTTTTACAC
ACCCGTATGATATTGCGCCG

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MIRQWPSPAKLNLFLYITGQREDGYHLLQTLFQFLDYGDTLTIDVRQDDAIHLLTPVAGVPDEQNLIVRAARLLQQHCDR
HGLNTAPRGAEISIEKRLPMGGGLGGGSSNAATVLVALNDLWRCGLSDEQLAELGLALGADVPVFVRGHAAFAEGIGERL
QPANPQEKWYLVAHPGVSIPTPVIFGDADLKRDTPVRPLNVLLQATYANDCEPIARKRFREVEQLVSWLLEYAPSRLTGT
GACVFAEFDTETAARQVLNQAPEWIRGFVARGVNVSPLHRIRSGQIEP

Sequences:

>Translated_288_residues
MIRQWPSPAKLNLFLYITGQREDGYHLLQTLFQFLDYGDTLTIDVRQDDAIHLLTPVAGVPDEQNLIVRAARLLQQHCDR
HGLNTAPRGAEISIEKRLPMGGGLGGGSSNAATVLVALNDLWRCGLSDEQLAELGLALGADVPVFVRGHAAFAEGIGERL
QPANPQEKWYLVAHPGVSIPTPVIFGDADLKRDTPVRPLNVLLQATYANDCEPIARKRFREVEQLVSWLLEYAPSRLTGT
GACVFAEFDTETAARQVLNQAPEWIRGFVARGVNVSPLHRIRSGQIEP
>Mature_288_residues
MIRQWPSPAKLNLFLYITGQREDGYHLLQTLFQFLDYGDTLTIDVRQDDAIHLLTPVAGVPDEQNLIVRAARLLQQHCDR
HGLNTAPRGAEISIEKRLPMGGGLGGGSSNAATVLVALNDLWRCGLSDEQLAELGLALGADVPVFVRGHAAFAEGIGERL
QPANPQEKWYLVAHPGVSIPTPVIFGDADLKRDTPVRPLNVLLQATYANDCEPIARKRFREVEQLVSWLLEYAPSRLTGT
GACVFAEFDTETAARQVLNQAPEWIRGFVARGVNVSPLHRIRSGQIEP

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=280, Percent_Identity=74.2857142857143, Blast_Score=409, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_SERP5 (A8GDA0)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001478218.1
- ProteinModelPortal:   A8GDA0
- SMR:   A8GDA0
- STRING:   A8GDA0
- GeneID:   5603257
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1987
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   LNLFLHV
- BioCyc:   SPRO399741:SPRO_1987-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31699; Mature: 31699

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRQWPSPAKLNLFLYITGQREDGYHLLQTLFQFLDYGDTLTIDVRQDDAIHLLTPVAGV
CCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCC
PDEQNLIVRAARLLQQHCDRHGLNTAPRGAEISIEKRLPMGGGLGGGSSNAATVLVALND
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEHH
LWRCGLSDEQLAELGLALGADVPVFVRGHAAFAEGIGERLQPANPQEKWYLVAHPGVSIP
HHHCCCCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCC
TPVIFGDADLKRDTPVRPLNVLLQATYANDCEPIARKRFREVEQLVSWLLEYAPSRLTGT
CCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GACVFAEFDTETAARQVLNQAPEWIRGFVARGVNVSPLHRIRSGQIEP
CEEEEEECCCHHHHHHHHHHCHHHHHHHHHCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MIRQWPSPAKLNLFLYITGQREDGYHLLQTLFQFLDYGDTLTIDVRQDDAIHLLTPVAGV
CCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCC
PDEQNLIVRAARLLQQHCDRHGLNTAPRGAEISIEKRLPMGGGLGGGSSNAATVLVALND
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEHH
LWRCGLSDEQLAELGLALGADVPVFVRGHAAFAEGIGERLQPANPQEKWYLVAHPGVSIP
HHHCCCCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCC
TPVIFGDADLKRDTPVRPLNVLLQATYANDCEPIARKRFREVEQLVSWLLEYAPSRLTGT
CCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GACVFAEFDTETAARQVLNQAPEWIRGFVARGVNVSPLHRIRSGQIEP
CEEEEEECCCHHHHHHHHHHCHHHHHHHHHCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA