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The map label for this gene is proP [H]

Identifier: 157370102

GI number: 157370102

Start: 2043740

End: 2045242

Strand: Reverse

Name: proP [H]

Synonym: Spro_1860

Alternate gene names: 157370102

Gene position: 2045242-2043740 (Counterclockwise)

Preceding gene: 157370103

Following gene: 157370096

Centisome position: 37.54

GC content: 53.49

Gene sequence:

>1503_bases
ATGAAGTTAAGAAAAAAACGTCATAAGCCGATGCATATTAATGACATCACCATTATCGACGACAGCAAGTTGAAAAAGGC
AATAACCGCGGCGGCATTAGGCAACGCGATGGAGTGGTTCGACTTTGGTGTTTATGGCTTTGTGGCCTACGCCCTCGGAC
AAGTATTTTTCCCTGGCGCCACGCCCGGCGTACAGATGATTGCCGCACTGGCAACCTTTTCCGTTCCCTTCCTGGTTCGT
CCGTTAGGCGGCATCTTCTTTGGTGCGATGGGCGATAAATTCGGTCGTCAAAAAGTCCTCTCCATCACCATCATTATCAT
GGCGGTGAGCACCTTCTGTATCGGGCTGATCCCCTCTTACGCTTCGATCGGTATTTGGGCACCGGTGCTGCTGCTGTTGG
CAAAATTGGCGCAGGGCTTCTCGGTCGGTGGCGAATACTCCGGCGCGGCAATCTTTGTCGCCGAATACTCACCGGACCGC
AAGCGTGGCTTTATGGGCAGTTGGCTTGATTTCGGCTCGATTGCCGGCTTCGTGCTGGGTGCCGGCGTTGTAGTGCTGAT
TTCCAGCATTGTTGGGGAAGCCAACTTCCTCGACTGGGGCTGGCGTATTCCGTTCTTCATCGCCGCGCCGTTGGGCCTGA
TTGGTCTGTACCTGCGACATGCGTTGGAAGAAACTCCGGCATTCCAGCAGCACGTGGAAAAAATGGAGAAGGAAGACCGC
AACGCCATCGAAAACCCGCCAAGAACTTCGTTCAAAGAGATTGCGACCAAGCACTGGAGAAGCCTGTTGGTGTGCGTCGG
TATCGTGATTTCGACCAACGTGACCTATTACATGTTGCTGACCTACATGCCGAGTTACCTGTCGCATAACCTGCACTACT
CGGAAGATCACGGCGTTCTGATTATTATCGCCGTCATGATCGGCATGCTGTTCGTACAGCCGGTGATCGGCATGACCAGT
GACCGCATTGGCCGTAAGCCCTTTATTATTGGCGGCAGTATCGGCCTGCTGGCGCTGGCGATCCCGTGTTTTATTTTGAT
CAACAGTAATGTGATTGGTTTGATTTTCGTCGGGCTGTTAGTCCTGGCGGTGCTGTTAAACTGCTTTACCGGCGTGATGG
CTTCGATTCTGCCGGCAATGTTCCCAACGCACATTCGCTACAGCGCATTGGCAATTTCGTTCAATATTTCGGTACTGGTG
GCCGGCGCAACGCCAACGGTGGCCGCCTGGTTGGTCGAGTCGTCCGGGAACCTGTATATGCCGGCTTACTACCTGATGAC
AGTCGCGGTAATAGGTCTCGCCACCGGTCTGTACATGAAGGAAACCGCCAATAAACCGCTGCGCGGTGCTACCCCAGCCG
CTTCGGACCGCTCAGAAGCCAAAGAGCTGCTGCAGGAGAACTACGACAACATCGAACAGAAAGTCGAAGATATCAACGAG
CAGATCGCCGAGCTGGAGAAGAAAAAACAGACGTTGGTCGACCAGCATCCAAAACTGGACTAA

Upstream 100 bases:

>100_bases
TGGCCGATCTCTGCGTATAGCGCTTAGATTAACCAAACTGATAACTGGGCAGAAGGATCGCGAATCTACGTACGACTAAA
ATTGATTAAGGGATGATTAT

Downstream 100 bases:

>100_bases
AACCATGCTCTAGTAAAAAGCCACCTCTTGAAGAGGTGGCTTTTTTATTGCGGAAAATGTGCTCCTGGTACCCCATCCCT
GGCGGTTGGTTTAGTTAACC

Product: proline/glycine betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MKLRKKRHKPMHINDITIIDDSKLKKAITAAALGNAMEWFDFGVYGFVAYALGQVFFPGATPGVQMIAALATFSVPFLVR
PLGGIFFGAMGDKFGRQKVLSITIIIMAVSTFCIGLIPSYASIGIWAPVLLLLAKLAQGFSVGGEYSGAAIFVAEYSPDR
KRGFMGSWLDFGSIAGFVLGAGVVVLISSIVGEANFLDWGWRIPFFIAAPLGLIGLYLRHALEETPAFQQHVEKMEKEDR
NAIENPPRTSFKEIATKHWRSLLVCVGIVISTNVTYYMLLTYMPSYLSHNLHYSEDHGVLIIIAVMIGMLFVQPVIGMTS
DRIGRKPFIIGGSIGLLALAIPCFILINSNVIGLIFVGLLVLAVLLNCFTGVMASILPAMFPTHIRYSALAISFNISVLV
AGATPTVAAWLVESSGNLYMPAYYLMTVAVIGLATGLYMKETANKPLRGATPAASDRSEAKELLQENYDNIEQKVEDINE
QIAELEKKKQTLVDQHPKLD

Sequences:

>Translated_500_residues
MKLRKKRHKPMHINDITIIDDSKLKKAITAAALGNAMEWFDFGVYGFVAYALGQVFFPGATPGVQMIAALATFSVPFLVR
PLGGIFFGAMGDKFGRQKVLSITIIIMAVSTFCIGLIPSYASIGIWAPVLLLLAKLAQGFSVGGEYSGAAIFVAEYSPDR
KRGFMGSWLDFGSIAGFVLGAGVVVLISSIVGEANFLDWGWRIPFFIAAPLGLIGLYLRHALEETPAFQQHVEKMEKEDR
NAIENPPRTSFKEIATKHWRSLLVCVGIVISTNVTYYMLLTYMPSYLSHNLHYSEDHGVLIIIAVMIGMLFVQPVIGMTS
DRIGRKPFIIGGSIGLLALAIPCFILINSNVIGLIFVGLLVLAVLLNCFTGVMASILPAMFPTHIRYSALAISFNISVLV
AGATPTVAAWLVESSGNLYMPAYYLMTVAVIGLATGLYMKETANKPLRGATPAASDRSEAKELLQENYDNIEQKVEDINE
QIAELEKKKQTLVDQHPKLD
>Mature_500_residues
MKLRKKRHKPMHINDITIIDDSKLKKAITAAALGNAMEWFDFGVYGFVAYALGQVFFPGATPGVQMIAALATFSVPFLVR
PLGGIFFGAMGDKFGRQKVLSITIIIMAVSTFCIGLIPSYASIGIWAPVLLLLAKLAQGFSVGGEYSGAAIFVAEYSPDR
KRGFMGSWLDFGSIAGFVLGAGVVVLISSIVGEANFLDWGWRIPFFIAAPLGLIGLYLRHALEETPAFQQHVEKMEKEDR
NAIENPPRTSFKEIATKHWRSLLVCVGIVISTNVTYYMLLTYMPSYLSHNLHYSEDHGVLIIIAVMIGMLFVQPVIGMTS
DRIGRKPFIIGGSIGLLALAIPCFILINSNVIGLIFVGLLVLAVLLNCFTGVMASILPAMFPTHIRYSALAISFNISVLV
AGATPTVAAWLVESSGNLYMPAYYLMTVAVIGLATGLYMKETANKPLRGATPAASDRSEAKELLQENYDNIEQKVEDINE
QIAELEKKKQTLVDQHPKLD

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=498, Percent_Identity=79.1164658634538, Blast_Score=847, Evalue=0.0,
Organism=Escherichia coli, GI1788292, Length=437, Percent_Identity=31.8077803203661, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1789941, Length=444, Percent_Identity=33.5585585585586, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1788942, Length=445, Percent_Identity=30.7865168539326, Blast_Score=188, Evalue=9e-49,
Organism=Escherichia coli, GI87082231, Length=483, Percent_Identity=24.223602484472, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI87082404, Length=391, Percent_Identity=26.0869565217391, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786229, Length=397, Percent_Identity=24.9370277078086, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323512, Length=549, Percent_Identity=24.408014571949, Blast_Score=81, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 54580; Mature: 54580

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRKKRHKPMHINDITIIDDSKLKKAITAAALGNAMEWFDFGVYGFVAYALGQVFFPGA
CCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
TPGVQMIAALATFSVPFLVRPLGGIFFGAMGDKFGRQKVLSITIIIMAVSTFCIGLIPSY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASIGIWAPVLLLLAKLAQGFSVGGEYSGAAIFVAEYSPDRKRGFMGSWLDFGSIAGFVLG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHCCCCCCHHHHHHHHHHHHH
AGVVVLISSIVGEANFLDWGWRIPFFIAAPLGLIGLYLRHALEETPAFQQHVEKMEKEDR
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
NAIENPPRTSFKEIATKHWRSLLVCVGIVISTNVTYYMLLTYMPSYLSHNLHYSEDHGVL
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHH
IIIAVMIGMLFVQPVIGMTSDRIGRKPFIIGGSIGLLALAIPCFILINSNVIGLIFVGLL
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VLAVLLNCFTGVMASILPAMFPTHIRYSALAISFNISVLVAGATPTVAAWLVESSGNLYM
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEECCCCHHHHHEEECCCCEEH
PAYYLMTVAVIGLATGLYMKETANKPLRGATPAASDRSEAKELLQENYDNIEQKVEDINE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QIAELEKKKQTLVDQHPKLD
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLRKKRHKPMHINDITIIDDSKLKKAITAAALGNAMEWFDFGVYGFVAYALGQVFFPGA
CCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
TPGVQMIAALATFSVPFLVRPLGGIFFGAMGDKFGRQKVLSITIIIMAVSTFCIGLIPSY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASIGIWAPVLLLLAKLAQGFSVGGEYSGAAIFVAEYSPDRKRGFMGSWLDFGSIAGFVLG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHCCCCCCHHHHHHHHHHHHH
AGVVVLISSIVGEANFLDWGWRIPFFIAAPLGLIGLYLRHALEETPAFQQHVEKMEKEDR
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
NAIENPPRTSFKEIATKHWRSLLVCVGIVISTNVTYYMLLTYMPSYLSHNLHYSEDHGVL
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHH
IIIAVMIGMLFVQPVIGMTSDRIGRKPFIIGGSIGLLALAIPCFILINSNVIGLIFVGLL
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VLAVLLNCFTGVMASILPAMFPTHIRYSALAISFNISVLVAGATPTVAAWLVESSGNLYM
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEECCCCHHHHHEEECCCCEEH
PAYYLMTVAVIGLATGLYMKETANKPLRGATPAASDRSEAKELLQENYDNIEQKVEDINE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QIAELEKKKQTLVDQHPKLD
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]