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The map label for this gene is pbuE [H]

Identifier: 157370071

GI number: 157370071

Start: 2011696

End: 2012868

Strand: Reverse

Name: pbuE [H]

Synonym: Spro_1829

Alternate gene names: 157370071

Gene position: 2012868-2011696 (Counterclockwise)

Preceding gene: 157370072

Following gene: 157370070

Centisome position: 36.94

GC content: 57.54

Gene sequence:

>1173_bases
ATGAAGTTGACGGACTATAACGGCCATTTTCTTACGCTGTGCCTGATGGCGACCGGCACCTTTGCCATCGGGACCGACGC
GTTTATTGTCGCCGGTATCCTCGACGAGATATCCGGCACCTTTGCCGTCAGCCCCGCGCAAGCGGGGCAGTTAATCTCGG
TATTTGCCCTGGCTTATATGCTGTTTGCCCCGCTGACCGCCTGGCTGCTGGGGAACCTCAATCGCAAACATATTTTGCAG
TTGGCCCTGGTGTTATTTATCGCCGGTAATCTGGTTTGTGCCTATGCCGACAGTTTTTTGCAAATGTCTGCCGGACGGGT
GCTGGCGGCGCTGGGCGCGGCTTGTTATACGCCACAGGCCGCAGCCTCTGCGGTTGGGCTGGTATCGGAGAAGCGCCGCG
GGCTGGCGATATCTATCGTTTACGGCGGCATGACGCTGGCCATCGCCCTTGGCATTCCCTTCGGCACCTTCCTGGCAAAG
TTGATCGGCTGGCGGGAAATCTTTTTGCTGATCGCCCTGTTGGGCGCGGTCTCACTGCTGGGGCTGTCGCTGGCGCTGAG
CACCATTGCGGCACCGGGTAAACACACGCTGAAAGAGCGGCTTGCTCCGCTGCGGCAAAAAGCGGTACTGACCACCCTGC
TGATTACCTTTTTCGCCGTCTGTTCCGAGCATATCGTTTACAGCTACGTGAGCGTATTGCTGAAAAACACCCAGTTTGGC
CCGGAGGCCATTTTGCCGATTGCGCTGCTGGTGTTCGGTATCGGCGCCGTTATCGGTAACTTCGTTTCCGGCCTGTTAAC
CGATACCTTCGGCAGCAAGTTTGTGCTGCTGTTTTCGGTATCGGTGCAAACCGTTTCGCTGTTCCTGCTGGCCTTTTACG
TCAGCTCCCCTTGGTGGGTACTGGCGATTTTTCTGGTCTGGGGCATTACCGGCTGGATGTATCTGGTGCCGATCCAGCAC
CACTTGTTGTCGCTGTCCAAACGCTTTGGCGCGCTGACCGTTTCACTCAACAGCTCGGTACTCTATGCCGGCATCGCGGC
GGGTGGCATGCTGGGTGGCCTGACGCTGTATGCGCTGCCGGCATTTTATCTCCCCTTGTTCTCGCTGCCGCTGGGGGCTA
TCGCCCTGCTGCTGACGCTGTTTTTCTTCCAGGGGGAAAAAACCAATGAGTGA

Upstream 100 bases:

>100_bases
TGCGCGTTACGCCGACCTCTGCCCATACTCATGAGGACGTGGAATATTTCCTGGCGGCGTTAAGCAAAGTCTGGGCTGCC
AATCAGTTACGGAGAGCAGG

Downstream 100 bases:

>100_bases
TCTGCCCCTCCCGGTGCGGTATGAGCAAAATGGCGGCATTGCCGTGATTACCCTGAACCGGCCGGAAAAAAAGAACGCCA
TCAATCTGGCGATGGCGCAA

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKLTDYNGHFLTLCLMATGTFAIGTDAFIVAGILDEISGTFAVSPAQAGQLISVFALAYMLFAPLTAWLLGNLNRKHILQ
LALVLFIAGNLVCAYADSFLQMSAGRVLAALGAACYTPQAAASAVGLVSEKRRGLAISIVYGGMTLAIALGIPFGTFLAK
LIGWREIFLLIALLGAVSLLGLSLALSTIAAPGKHTLKERLAPLRQKAVLTTLLITFFAVCSEHIVYSYVSVLLKNTQFG
PEAILPIALLVFGIGAVIGNFVSGLLTDTFGSKFVLLFSVSVQTVSLFLLAFYVSSPWWVLAIFLVWGITGWMYLVPIQH
HLLSLSKRFGALTVSLNSSVLYAGIAAGGMLGGLTLYALPAFYLPLFSLPLGAIALLLTLFFFQGEKTNE

Sequences:

>Translated_390_residues
MKLTDYNGHFLTLCLMATGTFAIGTDAFIVAGILDEISGTFAVSPAQAGQLISVFALAYMLFAPLTAWLLGNLNRKHILQ
LALVLFIAGNLVCAYADSFLQMSAGRVLAALGAACYTPQAAASAVGLVSEKRRGLAISIVYGGMTLAIALGIPFGTFLAK
LIGWREIFLLIALLGAVSLLGLSLALSTIAAPGKHTLKERLAPLRQKAVLTTLLITFFAVCSEHIVYSYVSVLLKNTQFG
PEAILPIALLVFGIGAVIGNFVSGLLTDTFGSKFVLLFSVSVQTVSLFLLAFYVSSPWWVLAIFLVWGITGWMYLVPIQH
HLLSLSKRFGALTVSLNSSVLYAGIAAGGMLGGLTLYALPAFYLPLFSLPLGAIALLLTLFFFQGEKTNE
>Mature_390_residues
MKLTDYNGHFLTLCLMATGTFAIGTDAFIVAGILDEISGTFAVSPAQAGQLISVFALAYMLFAPLTAWLLGNLNRKHILQ
LALVLFIAGNLVCAYADSFLQMSAGRVLAALGAACYTPQAAASAVGLVSEKRRGLAISIVYGGMTLAIALGIPFGTFLAK
LIGWREIFLLIALLGAVSLLGLSLALSTIAAPGKHTLKERLAPLRQKAVLTTLLITFFAVCSEHIVYSYVSVLLKNTQFG
PEAILPIALLVFGIGAVIGNFVSGLLTDTFGSKFVLLFSVSVQTVSLFLLAFYVSSPWWVLAIFLVWGITGWMYLVPIQH
HLLSLSKRFGALTVSLNSSVLYAGIAAGGMLGGLTLYALPAFYLPLFSLPLGAIALLLTLFFFQGEKTNE

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine, 6-mercaptopurine and 2-fluoroadenine. Therefore plays a role

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787947, Length=329, Percent_Identity=28.5714285714286, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786595, Length=255, Percent_Identity=27.4509803921569, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI145693198, Length=329, Percent_Identity=23.4042553191489, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787808, Length=229, Percent_Identity=25.764192139738, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41499; Mature: 41499

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTDYNGHFLTLCLMATGTFAIGTDAFIVAGILDEISGTFAVSPAQAGQLISVFALAYM
CCCCCCCCHHHHHHHHHHCCCEECCHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHH
LFAPLTAWLLGNLNRKHILQLALVLFIAGNLVCAYADSFLQMSAGRVLAALGAACYTPQA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AASAVGLVSEKRRGLAISIVYGGMTLAIALGIPFGTFLAKLIGWREIFLLIALLGAVSLL
HHHHHHHHHHHCCCEEEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLSLALSTIAAPGKHTLKERLAPLRQKAVLTTLLITFFAVCSEHIVYSYVSVLLKNTQFG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PEAILPIALLVFGIGAVIGNFVSGLLTDTFGSKFVLLFSVSVQTVSLFLLAFYVSSPWWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCHHH
LAIFLVWGITGWMYLVPIQHHLLSLSKRFGALTVSLNSSVLYAGIAAGGMLGGLTLYALP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
AFYLPLFSLPLGAIALLLTLFFFQGEKTNE
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKLTDYNGHFLTLCLMATGTFAIGTDAFIVAGILDEISGTFAVSPAQAGQLISVFALAYM
CCCCCCCCHHHHHHHHHHCCCEECCHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHH
LFAPLTAWLLGNLNRKHILQLALVLFIAGNLVCAYADSFLQMSAGRVLAALGAACYTPQA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AASAVGLVSEKRRGLAISIVYGGMTLAIALGIPFGTFLAKLIGWREIFLLIALLGAVSLL
HHHHHHHHHHHCCCEEEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLSLALSTIAAPGKHTLKERLAPLRQKAVLTTLLITFFAVCSEHIVYSYVSVLLKNTQFG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PEAILPIALLVFGIGAVIGNFVSGLLTDTFGSKFVLLFSVSVQTVSLFLLAFYVSSPWWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCHHH
LAIFLVWGITGWMYLVPIQHHLLSLSKRFGALTVSLNSSVLYAGIAAGGMLGGLTLYALP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
AFYLPLFSLPLGAIALLLTLFFFQGEKTNE
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]