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The map label for this gene is rutD

Identifier: 157370062

GI number: 157370062

Start: 2003724

End: 2004527

Strand: Reverse

Name: rutD

Synonym: Spro_1820

Alternate gene names: 157370062

Gene position: 2004527-2003724 (Counterclockwise)

Preceding gene: 157370063

Following gene: 157370061

Centisome position: 36.79

GC content: 60.2

Gene sequence:

>804_bases
ATGTATTTTGAGATTTTGGGAAAAGACACGCCGTTGGCACCCACGCTGGTGCTGTCTGCCGGGCTGGGTGGCGCAGGCAG
TTTTTGGCAACCGCAGATTAACGCGCTGGGCGAGCACTTCCGGGTAGTGGTTTACGATCACTTCGGTACCGCTCGCAGCA
AAGGGAGCGTGCCGGACGGCTACAGCATGGCCGATATGGCCGATGAAGTGGCGCAGTTGCTGCGTTCACTCAACGTCGAC
TGCTGTTACTTCGTCGGCCATGCACTGGGCGGCATGATTGGCCTGCAATTGGCGCTCACTCATCCGCAACTGGTCGAAAA
ACTGGTGGTGGTCAACGGCTGGCCGACGCTGGACAGCCAGACCCGCCGCTGCTTCAAAGTACGTCAGGATCTGTTGCTCA
ACAGCGGCGTGGAAGCCTATGTGCGCGCTCAGCCGCTGTTCCTGTTCCCCGCCGACTGGCTGTCGCAGCACTCCGCCTTG
CTGGATGAAGAGTTGCAGCACCAGACCGCACATTTTCAGGGTACCGAAAACCTGCTTCGCCGTCTGACTGCGCTGATGAA
TACCGATTTCCGTCCGCACCTGGCGGACATTACCACGCCAACCCTGGCGCTGTGCTCCCGCGACGATTTGCTGGTGCCCT
ATCACTGCTCGCATCAGTTGGCGGCCAGCCTGCCGAACGGCGAACTGGCCGAGATGGCCTACGGCGGCCATGCGATGAGC
GTGACCGACACCGAGCACTTTAACCGCATCCTGCTGGGTTGGCTGTTGAAAACGCAAAACGCCCAAACCCGCCTGCAACC
CTAG

Upstream 100 bases:

>100_bases
CTACTGCATTCAGTGCGGCCTGGTAAAACCGGACGCCCTGATTGAAATTGCCACCGTGGCCCATATCGGCCGCTGAGTCA
TTATCCGCAGAGGACTGAGC

Downstream 100 bases:

>100_bases
CCCGGAGACACAAAATGCAAAGCCAAACGCTGCTTGCCGATACGCCACTACAGTCGCCGTATGTAGAAAAACAGGACTTC
CGCGACGCCATGGCGCGCCT

Product: alpha/beta hydrolase fold domain-containing protein

Products: NA

Alternate protein names: Aminohydrolase

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MYFEILGKDTPLAPTLVLSAGLGGAGSFWQPQINALGEHFRVVVYDHFGTARSKGSVPDGYSMADMADEVAQLLRSLNVD
CCYFVGHALGGMIGLQLALTHPQLVEKLVVVNGWPTLDSQTRRCFKVRQDLLLNSGVEAYVRAQPLFLFPADWLSQHSAL
LDEELQHQTAHFQGTENLLRRLTALMNTDFRPHLADITTPTLALCSRDDLLVPYHCSHQLAASLPNGELAEMAYGGHAMS
VTDTEHFNRILLGWLLKTQNAQTRLQP

Sequences:

>Translated_267_residues
MYFEILGKDTPLAPTLVLSAGLGGAGSFWQPQINALGEHFRVVVYDHFGTARSKGSVPDGYSMADMADEVAQLLRSLNVD
CCYFVGHALGGMIGLQLALTHPQLVEKLVVVNGWPTLDSQTRRCFKVRQDLLLNSGVEAYVRAQPLFLFPADWLSQHSAL
LDEELQHQTAHFQGTENLLRRLTALMNTDFRPHLADITTPTLALCSRDDLLVPYHCSHQLAASLPNGELAEMAYGGHAMS
VTDTEHFNRILLGWLLKTQNAQTRLQP
>Mature_267_residues
MYFEILGKDTPLAPTLVLSAGLGGAGSFWQPQINALGEHFRVVVYDHFGTARSKGSVPDGYSMADMADEVAQLLRSLNVD
CCYFVGHALGGMIGLQLALTHPQLVEKLVVVNGWPTLDSQTRRCFKVRQDLLLNSGVEAYVRAQPLFLFPADWLSQHSAL
LDEELQHQTAHFQGTENLLRRLTALMNTDFRPHLADITTPTLALCSRDDLLVPYHCSHQLAASLPNGELAEMAYGGHAMS
VTDTEHFNRILLGWLLKTQNAQTRLQP

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family

Homologues:

Organism=Escherichia coli, GI1787244, Length=236, Percent_Identity=47.8813559322034, Blast_Score=231, Evalue=4e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTD_SERP5 (A8GCT3)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001478051.1
- ProteinModelPortal:   A8GCT3
- STRING:   A8GCT3
- GeneID:   5605394
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1820
- eggNOG:   COG0596
- HOGENOM:   HBG748003
- OMA:   LADITTP
- ProtClustDB:   CLSK964035
- BioCyc:   SPRO399741:SPRO_1820-MONOMER
- HAMAP:   MF_00832
- InterPro:   IPR000073
- InterPro:   IPR019913
- PRINTS:   PR00111
- TIGRFAMs:   TIGR03611

Pfam domain/function: PF00561 Abhydrolase_1

EC number: NA

Molecular weight: Translated: 29550; Mature: 29550

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFEILGKDTPLAPTLVLSAGLGGAGSFWQPQINALGEHFRVVVYDHFGTARSKGSVPDG
CEEEEECCCCCCCHHHHEECCCCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCCCCCCC
YSMADMADEVAQLLRSLNVDCCYFVGHALGGMIGLQLALTHPQLVEKLVVVNGWPTLDSQ
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEECCHHHHHHHHHCCCCCCCCHH
TRRCFKVRQDLLLNSGVEAYVRAQPLFLFPADWLSQHSALLDEELQHQTAHFQGTENLLR
HHHHHHHHHHHHHCCCCCEEEECCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
RLTALMNTDFRPHLADITTPTLALCSRDDLLVPYHCSHQLAASLPNGELAEMAYGGHAMS
HHHHHHCCCCCCCHHHCCCHHHHHCCCCCEEECCCCCHHHHHCCCCCCHHHHCCCCEEEE
VTDTEHFNRILLGWLLKTQNAQTRLQP
ECCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MYFEILGKDTPLAPTLVLSAGLGGAGSFWQPQINALGEHFRVVVYDHFGTARSKGSVPDG
CEEEEECCCCCCCHHHHEECCCCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCCCCCCC
YSMADMADEVAQLLRSLNVDCCYFVGHALGGMIGLQLALTHPQLVEKLVVVNGWPTLDSQ
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEECCHHHHHHHHHCCCCCCCCHH
TRRCFKVRQDLLLNSGVEAYVRAQPLFLFPADWLSQHSALLDEELQHQTAHFQGTENLLR
HHHHHHHHHHHHHCCCCCEEEECCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
RLTALMNTDFRPHLADITTPTLALCSRDDLLVPYHCSHQLAASLPNGELAEMAYGGHAMS
HHHHHHCCCCCCCHHHCCCHHHHHCCCCCEEECCCCCHHHHHCCCCCCHHHHCCCCEEEE
VTDTEHFNRILLGWLLKTQNAQTRLQP
ECCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA